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Protein

Electrogenic sodium bicarbonate cotransporter 4

Gene

Slc4a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates sodium- and bicarbonate-dependent electrogenic sodium bicarbonate cotransport, with a Na+:HCO3- stoichiometry of 2:1. May have a housekeeping function in regulating the pH of tissues in which it is expressed. May play a role in mediating Na+:HCO3- cotransport in hepatocytes and intrahepatic cholangiocytes. Also may be important in protecting the renal paranchyma from alterations in urine pH.By similarity1 Publication

GO - Molecular functioni

  • anion:anion antiporter activity Source: UniProtKB-KW
  • inorganic anion exchanger activity Source: RGD

GO - Biological processi

  • anion transport Source: RGD
  • bicarbonate transport Source: GO_Central
  • regulation of intracellular pH Source: GO_Central
  • regulation of pH Source: RGD
  • renal system process Source: GO_Central
  • sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Anion exchange, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-RNO-425381. Bicarbonate transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Electrogenic sodium bicarbonate cotransporter 4
Alternative name(s):
Solute carrier family 4 member 5
Gene namesi
Name:Slc4a5Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1303009. Slc4a5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 513CytoplasmicSequence analysisAdd BLAST513
Transmembranei514 – 536HelicalSequence analysisAdd BLAST23
Topological domaini537 – 547ExtracellularSequence analysisAdd BLAST11
Transmembranei548 – 579HelicalSequence analysisAdd BLAST32
Topological domaini580 – 598CytoplasmicSequence analysisAdd BLAST19
Transmembranei599 – 620HelicalSequence analysisAdd BLAST22
Topological domaini621 – 734ExtracellularSequence analysisAdd BLAST114
Transmembranei735 – 753HelicalSequence analysisAdd BLAST19
Topological domaini754 – 772CytoplasmicSequence analysisAdd BLAST19
Transmembranei773 – 792HelicalSequence analysisAdd BLAST20
Topological domaini793 – 820ExtracellularSequence analysisAdd BLAST28
Transmembranei821 – 839HelicalSequence analysisAdd BLAST19
Topological domaini840 – 858CytoplasmicSequence analysisAdd BLAST19
Transmembranei859 – 875HelicalSequence analysisAdd BLAST17
Topological domaini876 – 880ExtracellularSequence analysis5
Transmembranei881 – 900HelicalSequence analysisAdd BLAST20
Topological domaini901 – 920CytoplasmicSequence analysisAdd BLAST20
Transmembranei921 – 940HelicalSequence analysisAdd BLAST20
Topological domaini941 – 945ExtracellularSequence analysis5
Transmembranei946 – 966HelicalSequence analysisAdd BLAST21
Topological domaini967 – 992CytoplasmicSequence analysisAdd BLAST26
Transmembranei993 – 1010HelicalSequence analysisAdd BLAST18
Topological domaini1011 – 1015ExtracellularSequence analysis5
Transmembranei1016 – 1033HelicalSequence analysisAdd BLAST18
Topological domaini1034 – 1112CytoplasmicSequence analysisAdd BLAST79

GO - Cellular componenti

  • basolateral plasma membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: RGD
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289211 – 1112Electrogenic sodium bicarbonate cotransporter 4Add BLAST1112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi671 ↔ 673By similarity
Disulfide bondi717 ↔ 729By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ6RI88.
PRIDEiQ6RI88.

Expressioni

Tissue specificityi

Observed in hepatocytes and in the apical region of bile duct intrahepatic cholangiocytes of liver. Also observed in uroepithelium cells lining the outer pelvic wall of the kidney (at protein level). Highly expressed in colon, distal colon, liver, kidney and testis. Moderate expression in duodenum and stomach and weak expression in heart. In kidney, very weakly expressed in the inner medulla, but abundantly expressed in cortex and outer medulla in the medullary thick ascending and cortical thick ascending limbs of the loop of Henle.2 Publications

Gene expression databases

BgeeiENSRNOG00000010378.
ExpressionAtlasiQ6RI88. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014249.

Structurei

3D structure databases

ProteinModelPortaliQ6RI88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi432 – 465Gly-richSequence analysisAdd BLAST34

Sequence similaritiesi

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
GeneTreeiENSGT00760000119021.
HOGENOMiHOG000280684.
HOVERGENiHBG004326.
InParanoidiQ6RI88.
KOiK13857.
OMAiELIWTGR.
OrthoDBiEOG091G01FT.
PhylomeDBiQ6RI88.
TreeFamiTF313630.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 2 hits.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6RI88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVEEKAGVK KLEPTSYRRR HPEQDFPSIH IGFPVPGYSQ RKSDSKGHLS
60 70 80 90 100
GLQRVQWSLQ PDKSQQDLAG PDGIKASSLG GSVDFTKRIR SPAAEQLQDI
110 120 130 140 150
LGEEDEAPNP TLFTEMDTLQ HDGDQMEWKE SARWIKFEEK VEEGGERWSK
160 170 180 190 200
PHVSTLSLHS LFELRTCLQT GTVLLDLDSG SLPQIIDDVI EKQIEGGLLR
210 220 230 240 250
PELRERVSYV LLRKHRHQTK KPIHRSLADI GKSVSTTNRS SARSPSAGPT
260 270 280 290 300
LHHSTEDLRI RQSTSYGHLC HAQSRSMNDI SHTPNTDQRK NKFMKKIPKD
310 320 330 340 350
SEASNVLVGE VDFLDQPFIA FVRLAQSSML GGVTEVPVPT RFLFILLGPS
360 370 380 390 400
GRAKSYNEIG RAIATLMVDD LFSDVAYKAR NREDLIAGVD EFLDEVIVLP
410 420 430 440 450
PGEWDPNIRI EPPKKVPSAD KRKSVFSLAE LGQMNGSVGR SGASAGGGGS
460 470 480 490 500
GGGAGGSGAG GGGSGNEAEM PAMHEIGEEL IWTGRFFGGL RLDVKRKLPW
510 520 530 540 550
FPSDFYDGFH IQSISAVLFI YLGCITNAIT FGGLLGDATD NYQGVMESFL
560 570 580 590 600
GTAMAGSLFC LFSGQPLIIL SSTGPILIFE KLLFDFSKAN GLDYMEFRLW
610 620 630 640 650
IGLHSAIQCL ILVATDASFI IKYITRFTEE GFSTLISFIF IYDAIKKMIG
660 670 680 690 700
AFKYYPINTD FKPDSITTYK CECVAPDTVN TTTVNDSALL VPNTNMSVYT
710 720 730 740 750
PLNLTALDWS LLSKKECLSY GGRLLGSSCQ FVPDLALMSF ILFFGTYSMT
760 770 780 790 800
LTLKKFKFSR YFPTKVRTLV ADFSIVFSIL LFCGIDACFG LQTPKLHVPN
810 820 830 840 850
VIKPTRPDRG WFVAPFGKNP WWVYPASILP ALLVTILIFM DQQITAVIVN
860 870 880 890 900
RKENKLRKAA GYHLDLFWVG ILMALCSFMG LPWYVAATVI SIAHIDSLKM
910 920 930 940 950
ETETSAPGEQ PQFLGVREQR VTGVMVFILT GISVFLAPIL KYIPMPVLYG
960 970 980 990 1000
VFLYMGVASL NGIQFWDRCK LFLMPAKHQP DHAFLRHVPL RRIHLFTLVQ
1010 1020 1030 1040 1050
ILCLALLWIL KSTMAAIIFP VMILGLIIVR RLLDLIFSQH DLAWIDNILP
1060 1070 1080 1090 1100
EKEKKESDRK KRRKEVHENT DKEPQFLPPS VVKIPMEGIP SDPQNGIHCV
1110
GRKRSSSWSH SL
Length:1,112
Mass (Da):123,944
Last modified:July 5, 2004 - v1
Checksum:iBE5EF90A543DA35C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496959 mRNA. Translation: AAS98674.1.
RefSeqiNP_997677.1. NM_212512.1.
UniGeneiRn.214577.

Genome annotation databases

EnsembliENSRNOT00000014249; ENSRNOP00000014249; ENSRNOG00000010378.
GeneIDi297386.
KEGGirno:297386.
UCSCiRGD:1303009. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496959 mRNA. Translation: AAS98674.1.
RefSeqiNP_997677.1. NM_212512.1.
UniGeneiRn.214577.

3D structure databases

ProteinModelPortaliQ6RI88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014249.

Proteomic databases

PaxDbiQ6RI88.
PRIDEiQ6RI88.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014249; ENSRNOP00000014249; ENSRNOG00000010378.
GeneIDi297386.
KEGGirno:297386.
UCSCiRGD:1303009. rat.

Organism-specific databases

CTDi57835.
RGDi1303009. Slc4a5.

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
GeneTreeiENSGT00760000119021.
HOGENOMiHOG000280684.
HOVERGENiHBG004326.
InParanoidiQ6RI88.
KOiK13857.
OMAiELIWTGR.
OrthoDBiEOG091G01FT.
PhylomeDBiQ6RI88.
TreeFamiTF313630.

Enzyme and pathway databases

ReactomeiR-RNO-425381. Bicarbonate transporters.

Miscellaneous databases

PROiQ6RI88.

Gene expression databases

BgeeiENSRNOG00000010378.
ExpressionAtlasiQ6RI88. baseline and differential.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 2 hits.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS4A5_RAT
AccessioniPrimary (citable) accession number: Q6RI88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.