Reviewed, UniProtKB/Swiss-Prot Q6RI86 (TRPA1_RAT)

Names and origin

Protein names

Recommended name:
    Transient receptor potential cation channel subfamily A member 1
Alternative name(s):
    Ankyrin-like with transmembrane domains protein 1

Gene names

Name:	Trpa1
Synonyms:	Anktm1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Specifically expressed in a subset of nociceptive neurons. Expressed in dorsal root ganglia. Ref.2
Miscellaneous	Inhibited by ruthenium red, a potent blocker of TRPV channels By similarity.
Sequence similarities	Belongs to the transient receptor family. Contains 14 ANK repeats.

Ontologies

Keywords
Biological process	Ion transport Sensory transduction Transport
Cellular component	Membrane
Domain	ANK repeat Repeat Transmembrane
Molecular function	Ionic channel
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1125	1125	Transient receptor potential cation channel subfamily A member 1		PRO_0000215371
Regions
	Topological domain	1 – 719	719	Cytoplasmic Potential
	Transmembrane	720 – 740	21	1 Potential
	Topological domain	741 – 767	27	Extracellular Potential
	Transmembrane	768 – 788	21	2 Potential
	Topological domain	789 – 811	23	Cytoplasmic Potential
	Transmembrane	812 – 832	21	3 Potential
	Topological domain	833 – 834	2	Extracellular Potential
	Transmembrane	835 – 855	21	4 Potential
	Topological domain	856 – 876	21	Cytoplasmic Potential
	Transmembrane	877 – 897	21	5 Potential
	Topological domain	898 – 943	46	Extracellular Potential
	Transmembrane	944 – 964	21	6 Potential
	Topological domain	965 – 1125	161	Cytoplasmic Potential
	Repeat	63 – 94	32	ANK 1
	Repeat	98 – 127	30	ANK 2
	Repeat	131 – 161	31	ANK 3
	Repeat	165 – 194	30	ANK 4
	Repeat	198 – 227	30	ANK 5
	Repeat	239 – 268	30	ANK 6
	Repeat	272 – 301	30	ANK 7
	Repeat	309 – 338	30	ANK 8
	Repeat	342 – 371	30	ANK 9
	Repeat	413 – 442	30	ANK 10
	Repeat	446 – 475	30	ANK 11
	Repeat	482 – 511	30	ANK 12
	Repeat	514 – 543	30	ANK 13
	Repeat	548 – 577	30	ANK 14
Amino acid modifications
	Glycosylation	749	1	N-linked (GlcNAc...) Potential
	Glycosylation	755	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6RI86-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 32A6FB51EDCBB4B5	FASTA	1,125	128,602
	10 20 30 40 50 60 MKRSLRRVLR PEERKEVQGV VYRGVGKDMD CSKESFKVDI EGDMCRLEAF IKNRRKLSKY 70 80 90 100 110 120 EDENLCLLHH AAAEGQVELM QLIINGSSCE ALNVMDDYGN TPLHWAAEKN QVESVKFLLS 130 140 150 160 170 180 QGANPNLRNR NMMAPLHIAV QGMYNEVIKV LTEHKATNIN LEGENGNTAL MSTCAKDNSE 190 200 210 220 230 240 ALQILLEKGA KLCKSNKWGD YPVHQAAFSG AKRCMELILA YGEKTGYSRE AHINFVNHKK 250 260 270 280 290 300 ASPLHLAVQS GDLDMIKMCL DSGAHIDMME NAKCMALHFA ATQGATDIVK LMISSYTGSS 310 320 330 340 350 360 DIVNAVDGNQ ETLLHRASLF DHHDLADYLI SVGADINSTD SEGRSPLILA TASASWNIVN 370 380 390 400 410 420 LLLSKGAKVD IKDHLGRNFL HLTVQQPYGL RNLRPEFLQM QHIKELVMDE DNDGCTPLHY 430 440 450 460 470 480 ACRQGAPVSV NNLLRFNVSV HSKSKDKKSP LHFAASYGRI NTCQRLLQDI SDTRLLNEGD 490 500 510 520 530 540 LHGMTPLHLA AKNGHDKVVQ LLLKKGALFL SDHNGWTALH HASMGGYTQT MKVILDTNLK 550 560 570 580 590 600 CTDRLDEEGN TALHFAAREG HAKAVAMLLS YNADILLNKK QASFLHIALH NKRKEVVLTT 610 620 630 640 650 660 IRSKRWDECL QVFTHDSPSN RCPIMEMVEY LPECMKVLLD FCMIPSTEDK SCQDYHIEYN 670 680 690 700 710 720 FKYLQCPLSM TKKVTPTQDV IYEPLTILNV MVQHNRIELL NHPVCREYLL MKWCAYGFRA 730 740 750 760 770 780 HMMNLGSYCL GLIPMTLLVV KIQPGMAFNS TGIINETIST HEERINTLNS FPLKICMILV 790 800 810 820 830 840 FLSSIFGYCK EVVQIFQQKR NYFLDYNNAL EWVIYTTSMI FVLPLFLDIP AYMQWQCGAI 850 860 870 880 890 900 AIFFYWMNFL LYLQRFENCG IFIVMLEVIF KTLLRSTGVF IFLLLAFGLS FYVLLNFQDA 910 920 930 940 950 960 FSTPLLSLIQ TFSMMLGDIN YRDAFLEPLF RNELAYPVLT FGQLIAFTMF VPIVLMNLLI 970 980 990 1000 1010 1020 GLAVGDIAEV QKHASLKRIA MQVELHTNLE KKLPFWYLRK VDQRSTIVYP NRPRHGRMLR 1030 1040 1050 1060 1070 1080 FFHYFLSMQE TRQEAPNIDT CLEMEILKQK YRLKDLTSLL EKQHELIKLI IQKMEIISET 1090 1100 1110 1120 EDEDNHCSFQ DRFKKERLEQ MHSKWNFVLN AVKTKTHCSI SHPDI

References

[1]	"Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1." Jordt S.-E., Bautista D.M., Chuang H.-H., McKemy D.D., Zygmunt P.M., Hoegestaett E.D., Meng I.D., Julius D. Nature 427:260-265(2004) [PubMed: 14712238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: Sprague-Dawley. Tissue: Trigeminal ganglion.
[2]	"ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures." Story G.M., Peier A.M., Reeve A.J., Eid S.R., Mosbacher J., Hricik T.R., Earley T.J., Hergarden A.C., Anderson D.A., Hwang S.W., McIntyre P., Jegla T., Bevan S., Patapoutian A. Cell 112:819-829(2003) [PubMed: 12654248] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The power behind pain - Issue 82 of May 2007

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY496961 mRNA. Translation: AAS78661.1.
IPI	IPI00411253.
RefSeq	NP_997491.1.
UniGene	Rn.105247
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6RI86.
Genome annotation databases
Ensembl	ENSRNOT00000009874; ENSRNOP00000009874; ENSRNOG00000007354; Rattus norvegicus. [Genome view]
GeneID	312896.
KEGG	rno:312896.
UCSC	NM_207608. rat.
Organism-specific databases
CTD	312896.
RGD	1303284. Trpa1.
Phylogenomic databases
HOVERGEN	Q6RI86.
Gene expression databases
Genevestigator	Q6RI86.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR005821. Ion_trans. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 3 hits.
Pfam	PF00023. Ank. 14 hits. PF00520. Ion_trans. 1 hit. [Graphical view]
SMART	SM00248. ANK. 14 hits. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 9 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	665335.

Entry information

Entry name

TRPA1_RAT

Accession

Primary (citable) accession number: Q6RI86

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	July 5, 2004
Last modified:	November 24, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)