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Protein

Phosphoglycerate kinase 2

Gene

PGK2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.Curated

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391SubstrateBy similarity
Binding sitei123 – 1231SubstrateBy similarity
Binding sitei171 – 1711SubstrateBy similarity
Binding sitei220 – 2201ATPBy similarity
Binding sitei344 – 3441ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi373 – 3764ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. phosphoglycerate kinase activity Source: UniProtKB

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
  2. phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 2 (EC:2.7.2.3)
Alternative name(s):
Phosphoglycerate kinase, testis specific
Gene namesi
Name:PGK2Imported
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Unplaced

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 417416Phosphoglycerate kinase 2PRO_0000145838Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei6 – 61N6-succinyllysineBy similarity
Modified residuei11 – 111N6-acetyllysineBy similarity
Modified residuei75 – 751N6-acetyllysineBy similarity
Modified residuei76 – 761PhosphotyrosineBy similarity
Modified residuei86 – 861N6-acetyllysineBy similarity
Modified residuei91 – 911N6-acetyllysineBy similarity
Modified residuei97 – 971N6-acetyllysineBy similarity
Modified residuei131 – 1311N6-acetyllysineBy similarity
Modified residuei146 – 1461N6-acetyllysineBy similarity
Modified residuei191 – 1911N6-succinyllysineBy similarity
Modified residuei196 – 1961PhosphotyrosineBy similarity
Modified residuei199 – 1991N6-acetyllysineBy similarity
Modified residuei267 – 2671N6-acetyllysineBy similarity
Modified residuei291 – 2911N6-acetyllysineBy similarity
Modified residuei361 – 3611N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ6RI85.

Expressioni

Tissue specificityi

Testis specific.1 Publication

Developmental stagei

Expression is highest in the adult, lower in a 10 month old and very weak in an 8 week old.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6RI85.
SMRiQ6RI85. Positions 2-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 263Substrate bindingBy similarity
Regioni63 – 664Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Sequence Analysis

Phylogenomic databases

HOVERGENiHBG008177.
InParanoidiQ6RI85.
KOiK00927.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6RI85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSKKLTLD KLDVKGKRVI MRVDFNVPMK RNQVTNNQRI KASLPSIRYC
60 70 80 90 100
LDNGARSVVL MSHLGRPDGV AMPDKYSLEP VAAELKSLLG KDVLFLKDCV
110 120 130 140 150
GSEAEQACAN PPAGSVILLE NLRFHVEEEG KGQDPSGNKL KAEPDKVEAF
160 170 180 190 200
RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKASGFLM KKELDYFAKA
210 220 230 240 250
LENPERPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AFTFLKVLNN
260 270 280 290 300
MEIGASLFDK EGATIVKEIM AKAEKNRVNI TFPVDFVIAD KFEENAKVGQ
310 320 330 340 350
ATVASGIPAG WVALDCGPET NKKYAQVVAR AKLIVWNGPL GVFEWDAFAN
360 370 380 390 400
GTKALMDEIV KATSKGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL
410
ELLEGKVLPG VEALSNL
Length:417
Mass (Da):44,895
Last modified:January 23, 2007 - v3
Checksum:i1DB0048021528BA0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti324 – 3241Y → N in AAR89550 (PubMed:15599558).Curated

Polymorphismi

Contains at least 10 polymorphisms. Those that lead to amino acid substitutions may alter protein secondary structures and may influence male fertility.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti102 – 1021S → P in SNP-A; possible loss of CK2 phosphorylation site. 1 Publication
Natural varianti264 – 2641T → K in SNP-B; increased semen volume in ejaculate. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496962 mRNA. Translation: AAR88362.1.
AY500132 Genomic DNA. Translation: AAR89550.1.
RefSeqiNP_998947.1. NM_213782.1.
UniGeneiSsc.58731.

Genome annotation databases

GeneIDi396673.
KEGGissc:396673.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496962 mRNA. Translation: AAR88362.1.
AY500132 Genomic DNA. Translation: AAR89550.1.
RefSeqiNP_998947.1. NM_213782.1.
UniGeneiSsc.58731.

3D structure databases

ProteinModelPortaliQ6RI85.
SMRiQ6RI85. Positions 2-417.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6RI85.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396673.
KEGGissc:396673.

Organism-specific databases

CTDi5232.

Phylogenomic databases

HOVERGENiHBG008177.
InParanoidiQ6RI85.
KOiK00927.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of the porcine testis-specific phosphoglycerate kinase 2 (PGK2) gene and its association with male fertility."
    Chen K., Knorr C., Moser G., Gatphayak K., Brenig B.
    Mamm. Genome 15:996-1006(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, VARIANTS PRO-102 AND LYS-264.

Entry informationi

Entry nameiPGK2_PIG
AccessioniPrimary (citable) accession number: Q6RI85
Secondary accession number(s): Q6RFZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 59 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.