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Q6RI85 (PGK2_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase 2
EC=2.7.2.3
Alternative name(s):
Phosphoglycerate kinase, testis specific
Gene names
Name:PGK2
OrganismSus scrofa (Pig) [Complete proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.

Subunit structure

Monomer By similarity. UniProtKB P00559

Subcellular location

Cytoplasm By similarity UniProtKB P00559.

Tissue specificity

Testis specific. Ref.1

Developmental stage

Expression is highest in the adult, lower in a 10 month old and very weak in an 8 week old. Ref.1

Polymorphism

Contains at least 10 polymorphisms. Those that lead to amino acid substitutions may alter protein secondary structures and may influence male fertility. Ref.1

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from sequence or structural similarity. Source: UniProtKB

phosphoglycerate kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity UniProtKB P00559
Chain2 – 417416Phosphoglycerate kinase 2
PRO_0000145838

Regions

Nucleotide binding373 – 3764ATP By similarity
Region24 – 263Substrate binding By similarity
Region63 – 664Substrate binding By similarity

Sites

Binding site391Substrate By similarity
Binding site1231Substrate By similarity
Binding site1711Substrate By similarity
Binding site2201ATP By similarity UniProtKB P00559
Binding site3441ATP By similarity UniProtKB P00559

Amino acid modifications

Modified residue21N-acetylserine By similarity UniProtKB P00559
Modified residue911N6-acetyllysine By similarity
Modified residue1561N6-acetyllysine By similarity
Modified residue2671N6-acetyllysine By similarity

Natural variations

Natural variant1021S → P in SNP-A; possible loss of CK2 phosphorylation site. Ref.1
Natural variant2641T → K in SNP-B; increased semen volume in ejaculate. Ref.1

Experimental info

Sequence conflict3241Y → N in AAR89550. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6RI85 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 1DB0048021528BA0

FASTA41744,895
        10         20         30         40         50         60 
MSLSKKLTLD KLDVKGKRVI MRVDFNVPMK RNQVTNNQRI KASLPSIRYC LDNGARSVVL 

        70         80         90        100        110        120 
MSHLGRPDGV AMPDKYSLEP VAAELKSLLG KDVLFLKDCV GSEAEQACAN PPAGSVILLE 

       130        140        150        160        170        180 
NLRFHVEEEG KGQDPSGNKL KAEPDKVEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN 

       190        200        210        220        230        240 
LPQKASGFLM KKELDYFAKA LENPERPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM 

       250        260        270        280        290        300 
AFTFLKVLNN MEIGASLFDK EGATIVKEIM AKAEKNRVNI TFPVDFVIAD KFEENAKVGQ 

       310        320        330        340        350        360 
ATVASGIPAG WVALDCGPET NKKYAQVVAR AKLIVWNGPL GVFEWDAFAN GTKALMDEIV 

       370        380        390        400        410 
KATSKGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VEALSNL

« Hide

References

[1]"Molecular characterization of the porcine testis-specific phosphoglycerate kinase 2 (PGK2) gene and its association with male fertility."
Chen K., Knorr C., Moser G., Gatphayak K., Brenig B.
Mamm. Genome 15:996-1006(2004) [PubMed: 15599558] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, VARIANTS PRO-102 AND LYS-264.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY496962 mRNA. Translation: AAR88362.1.
AY500132 Genomic DNA. Translation: AAR89550.1.
RefSeqNP_998947.1. NM_213782.1.
UniGeneSsc.58731.

3D structure databases

ProteinModelPortalQ6RI85.
SMRQ6RI85. Positions 2-417.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396673.
KEGGssc:396673.

Organism-specific databases

CTD5232.

Phylogenomic databases

HOVERGENHBG008177.

Family and domain databases

InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
Gene3DG3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit.
G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
KOK00927.
PANTHERPTHR11406. PGK. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. PGK. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK2_PIG
AccessionPrimary (citable) accession number: Q6RI85
Secondary accession number(s): Q6RFZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: January 23, 2007
Last modified: November 16, 2011
This is version 43 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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