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Q6RI85 (PGK2_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase 2

EC=2.7.2.3
Alternative name(s):
Phosphoglycerate kinase, testis specific
Gene names
Name:PGK2
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.

Subunit structure

Monomer By similarity. UniProtKB P00559

Subcellular location

Cytoplasm By similarity UniProtKB P00559.

Tissue specificity

Testis specific. Ref.1

Developmental stage

Expression is highest in the adult, lower in a 10 month old and very weak in an 8 week old. Ref.1

Polymorphism

Contains at least 10 polymorphisms. Those that lead to amino acid substitutions may alter protein secondary structures and may influence male fertility. Ref.1

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from sequence or structural similarity. Source: UniProtKB

phosphoglycerate kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417Phosphoglycerate kinase 2
PRO_0000145838

Regions

Nucleotide binding373 – 3764ATP By similarity
Region24 – 263Substrate binding By similarity
Region63 – 664Substrate binding By similarity

Sites

Binding site391Substrate By similarity
Binding site1231Substrate By similarity
Binding site1711Substrate By similarity
Binding site2201ATP By similarity
Binding site3441ATP By similarity

Natural variations

Natural variant1021S → P in SNP-A; possible loss of CK2 phosphorylation site.
Natural variant2641T → K in SNP-B; increased semen volume in ejaculate.

Experimental info

Sequence conflict3241Y → N in AAR89550. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6RI85 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 1DB0048021528BA0

FASTA41744,895
        10         20         30         40         50         60 
MSLSKKLTLD KLDVKGKRVI MRVDFNVPMK RNQVTNNQRI KASLPSIRYC LDNGARSVVL 

        70         80         90        100        110        120 
MSHLGRPDGV AMPDKYSLEP VAAELKSLLG KDVLFLKDCV GSEAEQACAN PPAGSVILLE 

       130        140        150        160        170        180 
NLRFHVEEEG KGQDPSGNKL KAEPDKVEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN 

       190        200        210        220        230        240 
LPQKASGFLM KKELDYFAKA LENPERPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM 

       250        260        270        280        290        300 
AFTFLKVLNN MEIGASLFDK EGATIVKEIM AKAEKNRVNI TFPVDFVIAD KFEENAKVGQ 

       310        320        330        340        350        360 
ATVASGIPAG WVALDCGPET NKKYAQVVAR AKLIVWNGPL GVFEWDAFAN GTKALMDEIV 

       370        380        390        400        410 
KATSKGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VEALSNL 

« Hide

References

[1]"Molecular characterization of the porcine testis-specific phosphoglycerate kinase 2 (PGK2) gene and its association with male fertility."
Chen K., Knorr C., Moser G., Gatphayak K., Brenig B.
Mamm. Genome 15:996-1006(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, VARIANTS PRO-102 AND LYS-264.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY496962 mRNA. Translation: AAR88362.1.
AY500132 Genomic DNA. Translation: AAR89550.1.
RefSeqNP_998947.1. NM_213782.1.
UniGeneSsc.58731.

3D structure databases

ProteinModelPortalQ6RI85.
SMRQ6RI85. Positions 2-417.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ6RI85.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396673.
KEGGssc:396673.

Organism-specific databases

CTD5232.

Phylogenomic databases

HOVERGENHBG008177.
KOK00927.

Enzyme and pathway databases

UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK2_PIG
AccessionPrimary (citable) accession number: Q6RI85
Secondary accession number(s): Q6RFZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 54 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways