Q6RHW4 (HYAL1_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronidase-1
Short name=Hyal-1
EC=3.2.1.35

Alternative name(s):
Hyaluronoglucosaminidase-1

Gene names

Name:

HYAL1

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Secreted By similarity. Lysosome By similarity.
Tissue specificity	Highly expressed in spleen, kidney, and lung. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Lysosome Secreted
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cartilage development Inferred from sequence or structural similarity. Source: UniProtKB cellular response to UV-B Inferred from sequence or structural similarity. Source: UniProtKB cellular response to interleukin-1 Inferred from sequence or structural similarity. Source: UniProtKB cellular response to pH Inferred from sequence or structural similarity. Source: UniProtKB cellular response to platelet-derived growth factor stimulus Inferred from sequence or structural similarity. Source: UniProtKB hyaluronan biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB hyaluronan catabolic process Inferred from sequence or structural similarity. Source: UniProtKB inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of S phase of mitotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of angiogenesis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of epithelial cell migration Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of epithelial cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of hyaluranon cable assembly Inferred from sequence or structural similarity. Source: UniProtKB response to antibiotic Inferred from sequence or structural similarity. Source: UniProtKB response to reactive oxygen species Inferred from sequence or structural similarity. Source: UniProtKB response to virus Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasmic vesicle Inferred from sequence or structural similarity. Source: UniProtKB extracellular space Inferred from sequence or structural similarity. Source: UniProtKB hyaluranon cable Inferred from sequence or structural similarity. Source: UniProtKB lysosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	hyaluronan synthase activity Inferred from sequence or structural similarity. Source: UniProtKB hyalurononglucosaminidase activity Inferred from sequence or structural similarity. Source: UniProtKB transcription factor binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 435	414	Hyaluronidase-1		PRO_0000042625
Regions
	Domain	418 – 429	12	EGF-like
Sites
	Active site	131	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	107	1	N-linked (GlcNAc...) Potential
	Glycosylation	121	1	N-linked (GlcNAc...) Potential
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	350	1	N-linked (GlcNAc...) Potential
	Disulfide bond	43 ↔ 333		By similarity
	Disulfide bond	207 ↔ 221		By similarity
	Disulfide bond	358 ↔ 369		By similarity
	Disulfide bond	363 ↔ 418		By similarity
	Disulfide bond	420 ↔ 429		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6RHW4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5C5B7FB408B70B28	FASTA	435	48,507
	10 20 30 40 50 60 MAAHLLPICT LFLNLLSVAQ GSRDPVVLNR PFTTIWNANT QWCLKRHGVD VDVSVFEVVV 70 80 90 100 110 120 NPGQTFRGPN MTIFYSSQLG TYPYYTSAGE PVFGGLPQNA SLDVHLNRTF KDILAAMPES 130 140 150 160 170 180 NFSGLAVIDW EAWRPRWAFN WDAKDIYRQR SRALVQKQHP DWPAPWVEAA AQDQFQEAAQ 190 200 210 220 230 240 TWMAGTLKLG QTLRPHGLWG FYGFPDCYNY DFQSSNYTGQ CPPGVSAQND QLGWLWGQSR 250 260 270 280 290 300 ALYPSIYLPS ALEGTNKTQL YVQHRVNEAF RVAAAAGDPN LPVLPYAQIF HDMTNRLLSR 310 320 330 340 350 360 EELEHSLGES AAQGAAGVVL WVSWENTRTK ESCQSIKEYV DTTLGPFILN VTSGALLCSQ 370 380 390 400 410 420 AVCSGHGRCV RRPSHTEALP ILNPSSFSIK PTPGGGPLTL QGALSLKDRV QMAEEFQCRC 430 YPGWRGTWCE QQGTR

References

[1]	"Molecular characterization of porcine hyaluronidase genes 1, 2, and 3 clustered on SSC13q21." Gatphayak K., Knorr C., Beck J., Brenig B. Cytogenet. Genome Res. 106:98-106(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY497543 mRNA. Translation: AAR91599.1.
RefSeq	NP_999606.1. NM_214441.1.
UniGene	Ssc.26119.
3D structure databases
ProteinModelPortal	Q6RHW4.
SMR	Q6RHW4. Positions 23-432.
ModBase	Search...
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	404698.
KEGG	ssc:404698.
Organism-specific databases
CTD	3373.
Phylogenomic databases
eggNOG	NOG77606.
HOGENOM	HOG000015133.
HOVERGEN	HBG052053.
KO	K01197.
OrthoDB	EOG4VDPZD.
Enzyme and pathway databases
BRENDA	3.2.1.35. 6170.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR000742. EG-like_dom. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
PANTHER	PTHR11769. PTHR11769. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
SMART	SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYAL1_PIG

Accession

Primary (citable) accession number: Q6RHW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	July 5, 2004
Last modified:	March 6, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program