Reviewed, UniProtKB/Swiss-Prot Q6RHW2 (HYAL3_PIG)

Names and origin

Protein names

Recommended name:
    Hyaluronidase-3
      Short name=Hyal-3
    EC=3.2.1.35
Alternative name(s):
    Hyaluronoglucosaminidase-3

Gene names

Name:

HYAL3

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Secreted By similarity. Lysosome By similarity.
Tissue specificity	Highly expressed in bladder, spleen and liver. Expressed at low levels in the kidney. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Lysosome Secreted
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 419	403	Hyaluronidase-3		PRO_0000248202
Regions
	Domain	352 – 407	56	EGF-like
Sites
	Active site	129	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 331		By similarity
	Disulfide bond	205 ↔ 220		By similarity
	Disulfide bond	356 ↔ 367		By similarity
	Disulfide bond	361 ↔ 395		By similarity
	Disulfide bond	397 ↔ 406		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6RHW2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8035CDE3D04324AC	FASTA	419	46,393
	10 20 30 40 50 60 MTMQLGLALV LGVAMCLGCG QPLLRAPERP FCVLWNVPSA RCKARFGVHL PLEALGITAN 70 80 90 100 110 120 HGQRFHGQNI TIFYKSQLGL YPYFGPRGTA HNGGIPQAVS LDHHLARAAY QIHRSLRPGF 130 140 150 160 170 180 TGLAVLDWEE WCPLWAGNWG RRQAYQAASC AWAQRVYPNL DPQEQLCKAR AGFEEAARAL 190 200 210 220 230 240 MEDTLRLGRM LRPHGLWGFY HYPACGNGWH GTASNYTGHC HAAALARNTQ LYWLWAASSA 250 260 270 280 290 300 LFPSIYLPPG LPPAYHQAFV RYRLEEAFRV ALVGHPHPLP VLAYARLTHR NSGRFLSQDE 310 320 330 340 350 360 LVQTIGVSAA LGASGVVLWG DLSFSSSEEE CWHLRGYLVG TLGPYVINVT RAAMACSHQR 370 380 390 400 410 CHGHGRCAWQ DPGQLKVFLH LHPGGSPGAW ESFSCRCYWG WAGPTCQEPR PELGPEEAT

References

[1]	"Molecular characterization of porcine hyaluronidase genes 1, 2, and 3 clustered on SSC13q21." Gatphayak K., Knorr C., Beck J., Brenig B. Cytogenet. Genome Res. 106:98-106(2004) [PubMed: 15218248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Assignment of the porcine hyaluronidase-3 (HYAL3) gene to SSC13-->q21 by FISH and confirmation by hybrid panel analyses." Gatphayak K., Knorr C., Habermann F., Fries R., Brenig B. Cytogenet. Genome Res. 101:178-178(2003) [PubMed: 14619892] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-247.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY497545 mRNA. Translation: AAR91601.1. AY472019 Genomic DNA. Translation: AAR33037.1.
RefSeq	NP_999604.1.
UniGene	Ssc.26118
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Genome annotation databases
GeneID	404696.
KEGG	ssc:404696.
Organism-specific databases
CTD	404696.
Phylogenomic databases
HOVERGEN	Q6RHW2.
Enzyme and pathway databases
BRENDA	3.2.1.35. 249.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR017430. Glyco_hydro_56_Hyaluronidase. IPR001968. Glycoside_hydrolase_family_56. IPR018155. Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
ProDom	PD003549. Glyco_hydro_56. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYAL3_PIG

Accession

Primary (citable) accession number: Q6RHW2
Secondary accession number(s): Q6S3P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	September 1, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)