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Protein

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1

Gene

Magi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role as scaffolding protein at cell-cell junctions. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi103 – 110ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
Alternative name(s):
BAI1-associated protein 1
Short name:
BAP-1
Membrane-associated guanylate kinase inverted 1
Short name:
MAGI-1
Gene namesi
Name:Magi1
Synonyms:Baiap1, Bap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1203522. Magi1.

Subcellular locationi

Isoform 2 :
Isoform 1 :

GO - Cellular componenti

  • adherens junction Source: UniProtKB
  • bicellular tight junction Source: UniProtKB-SubCell
  • cell junction Source: MGI
  • cell projection Source: MGI
  • cytoplasm Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Nucleus, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000945901 – 1471Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1Add BLAST1471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei357PhosphoserineBy similarity1
Modified residuei722PhosphoserineBy similarity1
Modified residuei733PhosphoserineBy similarity1
Modified residuei792PhosphoserineCombined sources1
Modified residuei1051PhosphoserineBy similarity1
Modified residuei1341PhosphoserineBy similarity1
Modified residuei1392PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6RHR9.
PRIDEiQ6RHR9.

PTM databases

iPTMnetiQ6RHR9.
PhosphoSitePlusiQ6RHR9.

Expressioni

Tissue specificityi

Widely expressed, including kidney glomeruli.2 Publications

Gene expression databases

BgeeiENSMUSG00000045095.
CleanExiMM_BAP1.
ExpressionAtlasiQ6RHR9. baseline and differential.
GenevisibleiQ6RHR9. MM.

Interactioni

Subunit structurei

Interacts through its WW 2 domain with SYNPO and through its PDZ 5 domain with ACTN4. Interacts with cytoplasmic domain of BAI1. Interacts via its WW domains with DRPLA (By similarity). Interacts with ESAM, LRP2 and CXADR (PubMed:15383320, PubMed:11274227, PubMed:15304526). Isoform 2 interacts with CTNNB1 (PubMed:10772923). Interacts through its PDZ 1 domain with NET1 (PubMed:11350080). Interacts with ASIC3 and AMOT (By similarity) (PubMed:15317815). Interacts with FCHSD2 (By similarity). Interacts with IGSF5/JAM4 and through its PDZ 2 and 3 domains with NPHS1 forming a tripartite complex (By similarity). Interacts with DDN. May interact (via PDZ domain) with RAPGEF2 (By similarity). Interacts with DLL1 (PubMed:15509766). Interacts with KCNJ10 and possibly with KCNJ10/KCNJ16 heterodimer; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney tubular cells (By similarity).By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Net1Q9Z2066EBI-7440897,EBI-7840997

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200129. 3 interactors.
IntActiQ6RHR9. 1 interactor.
MINTiMINT-150460.
STRINGi10090.ENSMUSP00000086730.

Structurei

Secondary structure

11471
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi463 – 468Combined sources6
Beta strandi473 – 480Combined sources8
Beta strandi488 – 493Combined sources6
Helixi498 – 502Combined sources5
Beta strandi510 – 514Combined sources5
Helixi524 – 532Combined sources9
Beta strandi539 – 545Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I04X-ray2.15A/B463-546[»]
ProteinModelPortaliQ6RHR9.
SMRiQ6RHR9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6RHR9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 105PDZ 1PROSITE-ProRule annotationAdd BLAST89
Domaini96 – 287Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST192
Domaini300 – 333WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini359 – 392WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini464 – 546PDZ 2PROSITE-ProRule annotationAdd BLAST83
Domaini635 – 713PDZ 3PROSITE-ProRule annotationAdd BLAST79
Domaini833 – 915PDZ 4PROSITE-ProRule annotationAdd BLAST83
Domaini990 – 1074PDZ 5PROSITE-ProRule annotationAdd BLAST85
Domaini1132 – 1214PDZ 6PROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni990 – 1074Interaction with FCHSD2By similarityAdd BLAST85

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi237 – 242Poly-Glu6
Compositional biasi402 – 410Poly-Gln9
Compositional biasi970 – 980Poly-GlyAdd BLAST11
Compositional biasi1378 – 1381Poly-Arg4

Sequence similaritiesi

Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 6 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 2 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3209. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00650000092997.
HOGENOMiHOG000113463.
HOVERGENiHBG007091.
InParanoidiQ6RHR9.
KOiK05631.
OMAiIPDYQEQ.
OrthoDBiEOG091G0NSY.
PhylomeDBiQ6RHR9.
TreeFamiTF316816.

Family and domain databases

Gene3Di2.30.42.10. 6 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR030033. MAGI1.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR10316:SF12. PTHR10316:SF12. 3 hits.
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 5 hits.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 6 hits.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 6 hits.
SSF51045. SSF51045. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q6RHR9-1) [UniParc]FASTAAdd to basket
Also known as: MAGI1c alpha beta2 gamma, MAGI-1c

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKVIQKKNH WTGRVHECTV KRGPQGELGV TVLGGAEHGE FPYVGAVAAA
60 70 80 90 100
EAAGLPGGGE GPKLAEGELL LEVQGVRVSG LPRYDVLGVI DSCKEAVTFK
110 120 130 140 150
AVRQGGRLNK DLRHFLNQRF QKGSPDHELQ QTIRDNLYRH AVPCTTRSPR
160 170 180 190 200
EGEVPGVDYS FLTVKEFLDL EQSGTLLEVG TYEGNYYGTP KPPSQPVSGK
210 220 230 240 250
VITTDALHSL QSGSKQSTPK RTKSYNDMQN AGIVHPENEE EEDVPEMNSS
260 270 280 290 300
FTADSGDQDE HTLQEATLPP VNSSILAAPI TDPSQKFPQY LPLSAEDNLG
310 320 330 340 350
PLPENWEMAY TENGEVYFID HNTKTTSWLD PRCLNKQQKP LEECEDDEGV
360 370 380 390 400
HTEELDSELE LPAGWEKIED PVYGVYYVDH INRKTQYENP VLEAKRKKQL
410 420 430 440 450
EQQQQQQQPQ PPQPEEWTED HASVVPPVAP SHPPSNPEPA RETPLQGKPF
460 470 480 490 500
FTRNPSELKG KFIHTKLRKS SRGFGFTVVG GDEPDEFLQI KSLVLDGPAA
510 520 530 540 550
LDGKMETGDV IVSVNDTCVL GHTHAQVVKI FQSIPIGASV DLELCRGYPL
560 570 580 590 600
PFDPDDPNTS LVTSVAILDK EPIIVNGQET YDSPASHSSK TGKVSSMKDA
610 620 630 640 650
RPSSPADVAS NSSHGYPNDT VSLASSIATQ PELITVHIVK GPMGFGFTIA
660 670 680 690 700
DSPGGGGQRV KQIVDSPRCR GLKEGDLIVE VNKKNVQALT HNQVVDMLIE
710 720 730 740 750
CPKGSEVTLL VQRGGLPVPK KSPKSQPLER KDSQNSSQHS VSSHRSLHTA
760 770 780 790 800
SPSHGIQVLP EYLPADAPAP DQTDSSGQKK PDPFKIWAQS RSMYENRPMS
810 820 830 840 850
PSPASGLSKG ERDREINSTN FGECQIPDYQ EQDIFLWRKE TGFGFRILGG
860 870 880 890 900
NEPGEPIYIG HIVPLGAADT DGRLRSGDEL ICVDGTPVIG KSHQLVVQLM
910 920 930 940 950
QQAAKQGHVN LTVRRKVVFA VPKAENEVPS PASSHHSSNQ PASLTEEKRT
960 970 980 990 1000
PQGSQNSLNT VSSGSGSTSG IGSGGGGGSG VVSAVLQPYD VEIRRGENEG
1010 1020 1030 1040 1050
FGFVIVSSVS RPEAGTTFGR IIEGSPADRC GKLKVGDRIL AVNGCSITNK
1060 1070 1080 1090 1100
SHSDIVNLIK EAGNTVTLRI IPGDESSNAT LLTNAEKIAT ITTTHAPSQQ
1110 1120 1130 1140 1150
GTQETRTTTK PKQDSQFEFK GPQAAQEQDF YTVELERGAK GFGFSLRGGR
1160 1170 1180 1190 1200
EYNMDLYVLR LAEDGPAERC GKMRIGDEIL EINGETTKNM KHSRAIELIK
1210 1220 1230 1240 1250
NGGRRVRLFL RRGDGSVPEY DPSSDRNGPS TGAQGVPEVR PGPPDHRPHP
1260 1270 1280 1290 1300
ALESSYPPEL HKSSQHAEKR AHAKDPKGNR EHSKQPNEHH TWNGTSRKQD
1310 1320 1330 1340 1350
SGACRPKDRP PDAWREAQPE RTATNGSKRR SPEKRREGTR SADNTLERRE
1360 1370 1380 1390 1400
KHEKRREISP ERKRERSPTR RKDSSPSRRR RSLERLLDQR RSPERRRGGS
1410 1420 1430 1440 1450
PERRAKSTDR RRARSPERRR ERSLDKRNRD DKVGHREREE AGLKLEAGRS
1460 1470
PRNPPEQRRR PYKECSTDLS I
Length:1,471
Mass (Da):161,974
Last modified:July 5, 2004 - v1
Checksum:i6C780C71CAC37CB1
GO
Isoform 2 (identifier: Q6RHR9-2) [UniParc]FASTAAdd to basket
Also known as: MAGI-1b

The sequence of this isoform differs from the canonical sequence as follows:
     348-359: Missing.
     798-826: PMSPSPASGLSKGERDREINSTNFGECQI → L
     1019-1074: Missing.
     1221-1268: DPSSDRNGPS...ELHKSSQHAE → AMIPPKIAAC...PPPVHKVFRK
     1269-1471: Missing.

Show »
Length:1,172
Mass (Da):127,651
Checksum:i1255479BA421DAA0
GO
Isoform 3 (identifier: Q6RHR9-3) [UniParc]FASTAAdd to basket
Also known as: MAGI-1a

The sequence of this isoform differs from the canonical sequence as follows:
     1221-1236: DPSSDRNGPSTGAQGV → GGSNYENIPSFPGMTP
     1238-1268: Missing.
     1269-1471: Missing.

Show »
Length:1,237
Mass (Da):134,404
Checksum:i4B2D712E64126A55
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti726Missing in AAB91995 (PubMed:9395497).Curated1
Sequence conflicti1342A → D in AAB91997 (PubMed:9395497).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011673348 – 359Missing in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_011674798 – 826PMSPS…GECQI → L in isoform 2. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_0116751019 – 1074Missing in isoform 2. 1 PublicationAdd BLAST56
Alternative sequenceiVSP_0116761221 – 1268DPSSD…SQHAE → AMIPPKIAACMRNEKLGEAC FYLMGHNQTTTPAATGTAPP PVHKVFRK in isoform 2. 1 PublicationAdd BLAST48
Alternative sequenceiVSP_0116781221 – 1236DPSSD…GAQGV → GGSNYENIPSFPGMTP in isoform 3. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_0116791238 – 1268Missing in isoform 3. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_0116771269 – 1471Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST203

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY497557 mRNA. Translation: AAS77818.1.
AF027503 mRNA. Translation: AAB91995.1.
AF027504 mRNA. Translation: AAB91996.1.
AF027505 mRNA. Translation: AAB91997.1.
CCDSiCCDS20377.1. [Q6RHR9-1]
PIRiT42372.
RefSeqiNP_001025021.1. NM_001029850.4. [Q6RHR9-1]
NP_034497.1. NM_010367.3.
UniGeneiMm.217216.

Genome annotation databases

EnsembliENSMUST00000089317; ENSMUSP00000086730; ENSMUSG00000045095. [Q6RHR9-1]
GeneIDi14924.
KEGGimmu:14924.
UCSCiuc009czj.2. mouse. [Q6RHR9-1]
uc009czk.2. mouse. [Q6RHR9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY497557 mRNA. Translation: AAS77818.1.
AF027503 mRNA. Translation: AAB91995.1.
AF027504 mRNA. Translation: AAB91996.1.
AF027505 mRNA. Translation: AAB91997.1.
CCDSiCCDS20377.1. [Q6RHR9-1]
PIRiT42372.
RefSeqiNP_001025021.1. NM_001029850.4. [Q6RHR9-1]
NP_034497.1. NM_010367.3.
UniGeneiMm.217216.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I04X-ray2.15A/B463-546[»]
ProteinModelPortaliQ6RHR9.
SMRiQ6RHR9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200129. 3 interactors.
IntActiQ6RHR9. 1 interactor.
MINTiMINT-150460.
STRINGi10090.ENSMUSP00000086730.

PTM databases

iPTMnetiQ6RHR9.
PhosphoSitePlusiQ6RHR9.

Proteomic databases

PaxDbiQ6RHR9.
PRIDEiQ6RHR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089317; ENSMUSP00000086730; ENSMUSG00000045095. [Q6RHR9-1]
GeneIDi14924.
KEGGimmu:14924.
UCSCiuc009czj.2. mouse. [Q6RHR9-1]
uc009czk.2. mouse. [Q6RHR9-2]

Organism-specific databases

CTDi9223.
MGIiMGI:1203522. Magi1.

Phylogenomic databases

eggNOGiKOG3209. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00650000092997.
HOGENOMiHOG000113463.
HOVERGENiHBG007091.
InParanoidiQ6RHR9.
KOiK05631.
OMAiIPDYQEQ.
OrthoDBiEOG091G0NSY.
PhylomeDBiQ6RHR9.
TreeFamiTF316816.

Miscellaneous databases

ChiTaRSiMagi1. mouse.
EvolutionaryTraceiQ6RHR9.
PROiQ6RHR9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045095.
CleanExiMM_BAP1.
ExpressionAtlasiQ6RHR9. baseline and differential.
GenevisibleiQ6RHR9. MM.

Family and domain databases

Gene3Di2.30.42.10. 6 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR030033. MAGI1.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR10316:SF12. PTHR10316:SF12. 3 hits.
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 5 hits.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 6 hits.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 6 hits.
SSF51045. SSF51045. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAGI1_MOUSE
AccessioniPrimary (citable) accession number: Q6RHR9
Secondary accession number(s): O54893, O54894, O54895
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.