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Q6RFH5 (WDR74_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WD repeat-containing protein 74
Alternative name(s):
NOP seven-associated protein 1
Gene names
Name:WDR74
Synonyms:NSA1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Nucleusnucleolus Ref.4.

Sequence similarities

Contains 6 WD repeats.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
WD repeat
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA metabolic process

Inferred from electronic annotation. Source: Ensembl

blastocyst formation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentnucleolus

Inferred from direct assay Ref.4. Source: UniProtKB

nucleus

Inferred from direct assay. Source: HPA

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6RFH5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6RFH5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     308-326: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385WD repeat-containing protein 74
PRO_0000051428

Regions

Repeat40 – 8041WD 1
Repeat83 – 12240WD 2
Repeat128 – 16841WD 3
Repeat179 – 22042WD 4
Repeat224 – 26643WD 5
Repeat267 – 30640WD 6

Amino acid modifications

Modified residue2141Phosphoserine Ref.6
Modified residue3611Phosphoserine Ref.5 Ref.6

Natural variations

Alternative sequence308 – 32619Missing in isoform 2.
VSP_011957

Experimental info

Sequence conflict331F → L in BAA91610. Ref.2
Sequence conflict2291P → S in BAA91610. Ref.2
Sequence conflict297 – 2982RI → GT in BAA91610. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 8B4FA9730D8D5EC0

FASTA38542,441
        10         20         30         40         50         60 
MAAAAARWNH VWVGTETGIL KGVNLQRKQA ANFTAGGQPR REEAVSALCW GTGGETQMLV 

        70         80         90        100        110        120 
GCADRTVKHF STEDGIFQGQ RHCPGGEGMF RGLAQADGTL ITCVDSGILR VWHDKDKDTS 

       130        140        150        160        170        180 
SDPLLELRVG PGVCRMRQDP AHPHVVATGG KENALKIWDL QGSEEPVFRA KNVRNDWLDL 

       190        200        210        220        230        240 
RVPIWDQDIQ FLPGSQKLVT CTGYHQVRVY DPASPQRRPV LETTYGEYPL TAMTLTPGGN 

       250        260        270        280        290        300 
SVIVGNTHGQ LAEIDLRQGR LLGCLKGLAG SVRGLQCHPS KPLLASCGLD RVLRIHRIQN 

       310        320        330        340        350        360 
PRGLEHKVYL KSQLNCLLLS GRDNWEDEPQ EPQEPNKVPL EDTETDELWA SLEAAAKRKL 

       370        380 
SGLEQPQGAL QTRRRKKKRP GSTSP 

« Hide

Isoform 2 [UniParc].

Checksum: AB8D6910F3FF5C08
Show »

FASTA36640,208

References

« Hide 'large scale' references
[1]Eilbracht J., Hofmann A., Schmidt-Zachmann M.S.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Muscle.
[4]"Functional proteomic analysis of human nucleolus."
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214 AND SER-361, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY500828 mRNA. Translation: AAS48499.1.
AK001301 mRNA. Translation: BAA91610.1.
AK025383 mRNA. Translation: BAB15122.1.
AK292330 mRNA. Translation: BAF85019.1.
BC006351 mRNA. Translation: AAH06351.1.
CCDSCCDS44630.1. [Q6RFH5-1]
RefSeqNP_060563.2. NM_018093.2. [Q6RFH5-1]
XP_005274112.1. XM_005274055.1. [Q6RFH5-1]
UniGeneHs.654620.

3D structure databases

ProteinModelPortalQ6RFH5.
SMRQ6RFH5. Positions 45-72, 99-300.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120092. 13 interactions.
IntActQ6RFH5. 3 interactions.
MINTMINT-1433008.
STRING9606.ENSP00000278856.

PTM databases

PhosphoSiteQ6RFH5.

Polymorphism databases

DMDM55976441.

2D gel databases

SWISS-2DPAGEQ6RFH5.

Proteomic databases

MaxQBQ6RFH5.
PaxDbQ6RFH5.
PRIDEQ6RFH5.

Protocols and materials databases

DNASU54663.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000278856; ENSP00000278856; ENSG00000133316. [Q6RFH5-1]
ENST00000311713; ENSP00000308931; ENSG00000133316. [Q6RFH5-2]
ENST00000525239; ENSP00000432119; ENSG00000133316. [Q6RFH5-1]
ENST00000529106; ENSP00000435726; ENSG00000133316. [Q6RFH5-1]
GeneID54663.
KEGGhsa:54663.
UCSCuc001nvl.2. human. [Q6RFH5-2]
uc001nvm.2. human. [Q6RFH5-1]

Organism-specific databases

CTD54663.
GeneCardsGC11M062600.
HGNCHGNC:25529. WDR74.
HPAHPA037795.
HPA038419.
neXtProtNX_Q6RFH5.
PharmGKBPA142670578.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG329513.
HOGENOMHOG000006658.
HOVERGENHBG052672.
InParanoidQ6RFH5.
KOK14841.
OMAPQEPNKV.
PhylomeDBQ6RFH5.
TreeFamTF314666.

Enzyme and pathway databases

SignaLinkQ6RFH5.

Gene expression databases

ArrayExpressQ6RFH5.
BgeeQ6RFH5.
CleanExHS_WDR74.
GenevestigatorQ6RFH5.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 1 hit.
[Graphical view]
SMARTSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
ProtoNetSearch...

Other

ChiTaRSWDR74. human.
GenomeRNAi54663.
NextBio57206.
PROQ6RFH5.

Entry information

Entry nameWDR74_HUMAN
AccessionPrimary (citable) accession number: Q6RFH5
Secondary accession number(s): A8K8G5 expand/collapse secondary AC list , Q9BRC9, Q9H6X8, Q9NVY2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM