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Q6RFH5

- WDR74_HUMAN

UniProt

Q6RFH5 - WDR74_HUMAN

Protein

WD repeat-containing protein 74

Gene

WDR74

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    GO - Biological processi

    1. blastocyst formation Source: Ensembl
    2. RNA metabolic process Source: Ensembl

    Enzyme and pathway databases

    SignaLinkiQ6RFH5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    WD repeat-containing protein 74
    Alternative name(s):
    NOP seven-associated protein 1
    Gene namesi
    Name:WDR74
    Synonyms:NSA1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:25529. WDR74.

    Subcellular locationi

    Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142670578.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 385385WD repeat-containing protein 74PRO_0000051428Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei214 – 2141Phosphoserine1 Publication
    Modified residuei361 – 3611Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6RFH5.
    PaxDbiQ6RFH5.
    PRIDEiQ6RFH5.

    2D gel databases

    SWISS-2DPAGEQ6RFH5.

    PTM databases

    PhosphoSiteiQ6RFH5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6RFH5.
    BgeeiQ6RFH5.
    CleanExiHS_WDR74.
    GenevestigatoriQ6RFH5.

    Organism-specific databases

    HPAiHPA037795.
    HPA038419.

    Interactioni

    Protein-protein interaction databases

    BioGridi120092. 13 interactions.
    IntActiQ6RFH5. 3 interactions.
    MINTiMINT-1433008.
    STRINGi9606.ENSP00000278856.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6RFH5.
    SMRiQ6RFH5. Positions 45-72, 99-300.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati40 – 8041WD 1Add
    BLAST
    Repeati83 – 12240WD 2Add
    BLAST
    Repeati128 – 16841WD 3Add
    BLAST
    Repeati179 – 22042WD 4Add
    BLAST
    Repeati224 – 26643WD 5Add
    BLAST
    Repeati267 – 30640WD 6Add
    BLAST

    Sequence similaritiesi

    Contains 6 WD repeats.Curated

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiNOG329513.
    HOGENOMiHOG000006658.
    HOVERGENiHBG052672.
    InParanoidiQ6RFH5.
    KOiK14841.
    OMAiPQEPNKV.
    PhylomeDBiQ6RFH5.
    TreeFamiTF314666.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 1 hit.
    [Graphical view]
    SMARTiSM00320. WD40. 3 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6RFH5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAAAARWNH VWVGTETGIL KGVNLQRKQA ANFTAGGQPR REEAVSALCW    50
    GTGGETQMLV GCADRTVKHF STEDGIFQGQ RHCPGGEGMF RGLAQADGTL 100
    ITCVDSGILR VWHDKDKDTS SDPLLELRVG PGVCRMRQDP AHPHVVATGG 150
    KENALKIWDL QGSEEPVFRA KNVRNDWLDL RVPIWDQDIQ FLPGSQKLVT 200
    CTGYHQVRVY DPASPQRRPV LETTYGEYPL TAMTLTPGGN SVIVGNTHGQ 250
    LAEIDLRQGR LLGCLKGLAG SVRGLQCHPS KPLLASCGLD RVLRIHRIQN 300
    PRGLEHKVYL KSQLNCLLLS GRDNWEDEPQ EPQEPNKVPL EDTETDELWA 350
    SLEAAAKRKL SGLEQPQGAL QTRRRKKKRP GSTSP 385
    Length:385
    Mass (Da):42,441
    Last modified:July 5, 2004 - v1
    Checksum:i8B4FA9730D8D5EC0
    GO
    Isoform 2 (identifier: Q6RFH5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         308-326: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:366
    Mass (Da):40,208
    Checksum:iAB8D6910F3FF5C08
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti33 – 331F → L in BAA91610. (PubMed:14702039)Curated
    Sequence conflicti229 – 2291P → S in BAA91610. (PubMed:14702039)Curated
    Sequence conflicti297 – 2982RI → GT in BAA91610. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei308 – 32619Missing in isoform 2. 1 PublicationVSP_011957Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY500828 mRNA. Translation: AAS48499.1.
    AK001301 mRNA. Translation: BAA91610.1.
    AK025383 mRNA. Translation: BAB15122.1.
    AK292330 mRNA. Translation: BAF85019.1.
    BC006351 mRNA. Translation: AAH06351.1.
    CCDSiCCDS44630.1. [Q6RFH5-1]
    RefSeqiNP_060563.2. NM_018093.2. [Q6RFH5-1]
    XP_005274112.1. XM_005274055.1. [Q6RFH5-1]
    UniGeneiHs.654620.

    Genome annotation databases

    EnsembliENST00000278856; ENSP00000278856; ENSG00000133316. [Q6RFH5-1]
    ENST00000311713; ENSP00000308931; ENSG00000133316. [Q6RFH5-2]
    ENST00000525239; ENSP00000432119; ENSG00000133316. [Q6RFH5-1]
    ENST00000529106; ENSP00000435726; ENSG00000133316. [Q6RFH5-1]
    GeneIDi54663.
    KEGGihsa:54663.
    UCSCiuc001nvl.2. human. [Q6RFH5-2]
    uc001nvm.2. human. [Q6RFH5-1]

    Polymorphism databases

    DMDMi55976441.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY500828 mRNA. Translation: AAS48499.1 .
    AK001301 mRNA. Translation: BAA91610.1 .
    AK025383 mRNA. Translation: BAB15122.1 .
    AK292330 mRNA. Translation: BAF85019.1 .
    BC006351 mRNA. Translation: AAH06351.1 .
    CCDSi CCDS44630.1. [Q6RFH5-1 ]
    RefSeqi NP_060563.2. NM_018093.2. [Q6RFH5-1 ]
    XP_005274112.1. XM_005274055.1. [Q6RFH5-1 ]
    UniGenei Hs.654620.

    3D structure databases

    ProteinModelPortali Q6RFH5.
    SMRi Q6RFH5. Positions 45-72, 99-300.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120092. 13 interactions.
    IntActi Q6RFH5. 3 interactions.
    MINTi MINT-1433008.
    STRINGi 9606.ENSP00000278856.

    PTM databases

    PhosphoSitei Q6RFH5.

    Polymorphism databases

    DMDMi 55976441.

    2D gel databases

    SWISS-2DPAGE Q6RFH5.

    Proteomic databases

    MaxQBi Q6RFH5.
    PaxDbi Q6RFH5.
    PRIDEi Q6RFH5.

    Protocols and materials databases

    DNASUi 54663.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000278856 ; ENSP00000278856 ; ENSG00000133316 . [Q6RFH5-1 ]
    ENST00000311713 ; ENSP00000308931 ; ENSG00000133316 . [Q6RFH5-2 ]
    ENST00000525239 ; ENSP00000432119 ; ENSG00000133316 . [Q6RFH5-1 ]
    ENST00000529106 ; ENSP00000435726 ; ENSG00000133316 . [Q6RFH5-1 ]
    GeneIDi 54663.
    KEGGi hsa:54663.
    UCSCi uc001nvl.2. human. [Q6RFH5-2 ]
    uc001nvm.2. human. [Q6RFH5-1 ]

    Organism-specific databases

    CTDi 54663.
    GeneCardsi GC11M062600.
    HGNCi HGNC:25529. WDR74.
    HPAi HPA037795.
    HPA038419.
    neXtProti NX_Q6RFH5.
    PharmGKBi PA142670578.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG329513.
    HOGENOMi HOG000006658.
    HOVERGENi HBG052672.
    InParanoidi Q6RFH5.
    KOi K14841.
    OMAi PQEPNKV.
    PhylomeDBi Q6RFH5.
    TreeFami TF314666.

    Enzyme and pathway databases

    SignaLinki Q6RFH5.

    Miscellaneous databases

    ChiTaRSi WDR74. human.
    GenomeRNAii 54663.
    NextBioi 57206.
    PROi Q6RFH5.

    Gene expression databases

    ArrayExpressi Q6RFH5.
    Bgeei Q6RFH5.
    CleanExi HS_WDR74.
    Genevestigatori Q6RFH5.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 1 hit.
    [Graphical view ]
    SMARTi SM00320. WD40. 3 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Eilbracht J., Hofmann A., Schmidt-Zachmann M.S.
      Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Muscle.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214 AND SER-361, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiWDR74_HUMAN
    AccessioniPrimary (citable) accession number: Q6RFH5
    Secondary accession number(s): A8K8G5
    , Q9BRC9, Q9H6X8, Q9NVY2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3