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Protein

WD repeat-containing protein 74

Gene

WDR74

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  1. blastocyst formation Source: Ensembl
  2. RNA metabolic process Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

SignaLinkiQ6RFH5.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein 74
Alternative name(s):
NOP seven-associated protein 1
Gene namesi
Name:WDR74
Synonyms:NSA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:25529. WDR74.

Subcellular locationi

  1. Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. nucleolus Source: UniProtKB
  2. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670578.

Polymorphism and mutation databases

BioMutaiWDR74.
DMDMi55976441.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385WD repeat-containing protein 74PRO_0000051428Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei214 – 2141Phosphoserine1 Publication
Modified residuei361 – 3611Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6RFH5.
PaxDbiQ6RFH5.
PRIDEiQ6RFH5.

2D gel databases

SWISS-2DPAGEQ6RFH5.

PTM databases

PhosphoSiteiQ6RFH5.

Expressioni

Gene expression databases

BgeeiQ6RFH5.
CleanExiHS_WDR74.
ExpressionAtlasiQ6RFH5. baseline and differential.
GenevestigatoriQ6RFH5.

Organism-specific databases

HPAiHPA037795.
HPA038419.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RCHY1Q96PM53EBI-722366,EBI-947779

Protein-protein interaction databases

BioGridi120092. 16 interactions.
IntActiQ6RFH5. 4 interactions.
MINTiMINT-1433008.
STRINGi9606.ENSP00000278856.

Structurei

3D structure databases

ProteinModelPortaliQ6RFH5.
SMRiQ6RFH5. Positions 99-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati40 – 8041WD 1Add
BLAST
Repeati83 – 12240WD 2Add
BLAST
Repeati128 – 16841WD 3Add
BLAST
Repeati179 – 22042WD 4Add
BLAST
Repeati224 – 26643WD 5Add
BLAST
Repeati267 – 30640WD 6Add
BLAST

Sequence similaritiesi

Contains 6 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiNOG329513.
GeneTreeiENSGT00390000015119.
HOGENOMiHOG000006658.
HOVERGENiHBG052672.
InParanoidiQ6RFH5.
KOiK14841.
OMAiPQEPNKV.
PhylomeDBiQ6RFH5.
TreeFamiTF314666.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6RFH5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAARWNH VWVGTETGIL KGVNLQRKQA ANFTAGGQPR REEAVSALCW
60 70 80 90 100
GTGGETQMLV GCADRTVKHF STEDGIFQGQ RHCPGGEGMF RGLAQADGTL
110 120 130 140 150
ITCVDSGILR VWHDKDKDTS SDPLLELRVG PGVCRMRQDP AHPHVVATGG
160 170 180 190 200
KENALKIWDL QGSEEPVFRA KNVRNDWLDL RVPIWDQDIQ FLPGSQKLVT
210 220 230 240 250
CTGYHQVRVY DPASPQRRPV LETTYGEYPL TAMTLTPGGN SVIVGNTHGQ
260 270 280 290 300
LAEIDLRQGR LLGCLKGLAG SVRGLQCHPS KPLLASCGLD RVLRIHRIQN
310 320 330 340 350
PRGLEHKVYL KSQLNCLLLS GRDNWEDEPQ EPQEPNKVPL EDTETDELWA
360 370 380
SLEAAAKRKL SGLEQPQGAL QTRRRKKKRP GSTSP
Length:385
Mass (Da):42,441
Last modified:July 5, 2004 - v1
Checksum:i8B4FA9730D8D5EC0
GO
Isoform 2 (identifier: Q6RFH5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-326: Missing.

Note: No experimental confirmation available.

Show »
Length:366
Mass (Da):40,208
Checksum:iAB8D6910F3FF5C08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331F → L in BAA91610 (PubMed:14702039).Curated
Sequence conflicti229 – 2291P → S in BAA91610 (PubMed:14702039).Curated
Sequence conflicti297 – 2982RI → GT in BAA91610 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei308 – 32619Missing in isoform 2. 1 PublicationVSP_011957Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY500828 mRNA. Translation: AAS48499.1.
AK001301 mRNA. Translation: BAA91610.1.
AK025383 mRNA. Translation: BAB15122.1.
AK292330 mRNA. Translation: BAF85019.1.
BC006351 mRNA. Translation: AAH06351.1.
CCDSiCCDS44630.1. [Q6RFH5-1]
RefSeqiNP_060563.2. NM_018093.2. [Q6RFH5-1]
XP_005274112.1. XM_005274055.1. [Q6RFH5-1]
UniGeneiHs.654620.

Genome annotation databases

EnsembliENST00000278856; ENSP00000278856; ENSG00000133316. [Q6RFH5-1]
ENST00000311713; ENSP00000308931; ENSG00000133316. [Q6RFH5-2]
ENST00000525239; ENSP00000432119; ENSG00000133316. [Q6RFH5-1]
ENST00000529106; ENSP00000435726; ENSG00000133316. [Q6RFH5-1]
GeneIDi54663.
KEGGihsa:54663.
UCSCiuc001nvl.2. human. [Q6RFH5-2]
uc001nvm.2. human. [Q6RFH5-1]

Polymorphism and mutation databases

BioMutaiWDR74.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY500828 mRNA. Translation: AAS48499.1.
AK001301 mRNA. Translation: BAA91610.1.
AK025383 mRNA. Translation: BAB15122.1.
AK292330 mRNA. Translation: BAF85019.1.
BC006351 mRNA. Translation: AAH06351.1.
CCDSiCCDS44630.1. [Q6RFH5-1]
RefSeqiNP_060563.2. NM_018093.2. [Q6RFH5-1]
XP_005274112.1. XM_005274055.1. [Q6RFH5-1]
UniGeneiHs.654620.

3D structure databases

ProteinModelPortaliQ6RFH5.
SMRiQ6RFH5. Positions 99-300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120092. 16 interactions.
IntActiQ6RFH5. 4 interactions.
MINTiMINT-1433008.
STRINGi9606.ENSP00000278856.

PTM databases

PhosphoSiteiQ6RFH5.

Polymorphism and mutation databases

BioMutaiWDR74.
DMDMi55976441.

2D gel databases

SWISS-2DPAGEQ6RFH5.

Proteomic databases

MaxQBiQ6RFH5.
PaxDbiQ6RFH5.
PRIDEiQ6RFH5.

Protocols and materials databases

DNASUi54663.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000278856; ENSP00000278856; ENSG00000133316. [Q6RFH5-1]
ENST00000311713; ENSP00000308931; ENSG00000133316. [Q6RFH5-2]
ENST00000525239; ENSP00000432119; ENSG00000133316. [Q6RFH5-1]
ENST00000529106; ENSP00000435726; ENSG00000133316. [Q6RFH5-1]
GeneIDi54663.
KEGGihsa:54663.
UCSCiuc001nvl.2. human. [Q6RFH5-2]
uc001nvm.2. human. [Q6RFH5-1]

Organism-specific databases

CTDi54663.
GeneCardsiGC11M062600.
HGNCiHGNC:25529. WDR74.
HPAiHPA037795.
HPA038419.
neXtProtiNX_Q6RFH5.
PharmGKBiPA142670578.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG329513.
GeneTreeiENSGT00390000015119.
HOGENOMiHOG000006658.
HOVERGENiHBG052672.
InParanoidiQ6RFH5.
KOiK14841.
OMAiPQEPNKV.
PhylomeDBiQ6RFH5.
TreeFamiTF314666.

Enzyme and pathway databases

SignaLinkiQ6RFH5.

Miscellaneous databases

ChiTaRSiWDR74. human.
GenomeRNAii54663.
NextBioi57206.
PROiQ6RFH5.

Gene expression databases

BgeeiQ6RFH5.
CleanExiHS_WDR74.
ExpressionAtlasiQ6RFH5. baseline and differential.
GenevestigatoriQ6RFH5.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Eilbracht J., Hofmann A., Schmidt-Zachmann M.S.
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Muscle.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214 AND SER-361, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiWDR74_HUMAN
AccessioniPrimary (citable) accession number: Q6RFH5
Secondary accession number(s): A8K8G5
, Q9BRC9, Q9H6X8, Q9NVY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: July 5, 2004
Last modified: April 29, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.