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Protein

Rho GTPase-activating protein 20

Gene

Arhgap20

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB-KW
  • Rho GTPase binding Source: RGD

GO - Biological processi

  • regulation of Rho protein signal transduction Source: RGD
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-RNO-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 20
Alternative name(s):
RA and RhoGAP domain-containing protein
Short name:
RARhoGAP
Rho-type GTPase-activating protein 20
Gene namesi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1303160. Arhgap20.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002830881 – 1182Rho GTPase-activating protein 20Add BLAST1182

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei46PhosphoserineCombined sources1
Modified residuei704PhosphoserineBy similarity1
Modified residuei730PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6REY9.
PRIDEiQ6REY9.

PTM databases

iPTMnetiQ6REY9.
PhosphoSitePlusiQ6REY9.

Expressioni

Tissue specificityi

Highest expression is found in testis. Ubiquitously expressed in extragonadal tissues.1 Publication

Developmental stagei

Found to be expressed in developing spermatocytes, but not in terminally differentiated spermatozoa.1 Publication

Gene expression databases

BgeeiENSRNOG00000025624.
GenevisibleiQ6REY9. RN.

Interactioni

GO - Molecular functioni

  • Rho GTPase binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034071.

Structurei

3D structure databases

ProteinModelPortaliQ6REY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 187PHAdd BLAST102
Domaini194 – 283Ras-associatingPROSITE-ProRule annotationAdd BLAST90
Domaini365 – 551Rho-GAPPROSITE-ProRule annotationAdd BLAST187

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi910 – 1010Ser-richAdd BLAST101

Sequence similaritiesi

Contains 1 PH domain.Curated
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4724. Eukaryota.
ENOG410YV4E. LUCA.
GeneTreeiENSGT00530000063299.
HOGENOMiHOG000048702.
HOVERGENiHBG061748.
InParanoidiQ6REY9.
KOiK20641.
OMAiEQLPREM.
OrthoDBiEOG091G02C4.
PhylomeDBiQ6REY9.
TreeFamiTF331062.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6REY9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAMSPQQDA LGAQPGRSSS LTGMSRIAGG PGTKKKMKTL AERRRSAPSL
60 70 80 90 100
ILDKALQKRP STRDSHSASI DTCAFLSSFM CSSRTLLIDG PVELKRGLQR
110 120 130 140 150
QERHLFLFND LFVSAKIKYN NNFKIKNKVK LADMWTASCV DEVGEGNTNA
160 170 180 190 200
LKSFVLGWPT VNFVATFSSP EQKDKWLSLL QRYIALEKEK DYPKSIPLKI
210 220 230 240 250
FAKDIGNCAY FKTISVMNSD TASEVINMSL QMLGITGSER DYQLWVNSGK
260 270 280 290 300
EAAPYPLIGH EYPYGIKMSH LRDTALLTQG SKDSASPSQL QEPFLMEQLP
310 320 330 340 350
REMQCQFILK PTRLATAQQL SDSSHKTYKR RRSIINWAFW RGSSTHLDNL
360 370 380 390 400
PVSPTSPMPG QLFGVSLPDI CENDNLPKPI LDMLSFLNQK GPLTKGIFRQ
410 420 430 440 450
SANMKSCREL KEKLNSGIEV HLDCESIFVI ASVLKDFLRN IPESIFSSDL
460 470 480 490 500
YDHWVCVMDQ GNDEEKINTI QRLLDQLPRA NVVFLRYLFG VLHNIEQHSL
510 520 530 540 550
SNQMTAFNLA VCIAPSILWP PASSSPELEN EFTKKVSLLI QFLIENCCRI
560 570 580 590 600
FGEEITSLLG ELSERSDREH TPDTSCFQLN DSSYDSLENE LNEDADAPCS
610 620 630 640 650
DLVKRLGQGS RSMDSVLTLS DYDLEPPEAE GLLTLSNFDL DQSKEEHIRT
660 670 680 690 700
KQPLETKPVS VFVAYRKVSL GEHTRAPDGP GTPSCLPATA SDARKVFRRH
710 720 730 740 750
RRSSEPSIDY LDAKLSYLRE FYQKKLRKSS CDAVLSRKDE DYLKQTQPQK
760 770 780 790 800
KGDQRCFKQS SVTGTDVSKR NTANENIKKK SLSGHEGIQE TPFTKSKPVA
810 820 830 840 850
ISVASYMSSQ DHSWEQPFEA DACRFSPPHI ADAQKSSRAH RRCSEPSIDD
860 870 880 890 900
QNYKLSYLRG VYSKKQSKTS CEAGLLHGEE DYLKRHKSLQ MEGQKLINQS
910 920 930 940 950
LVMGIEVGKS SSSTNQSTEK VLPPRLNLCP RASYSSLSSP GTSPSGSSVS
960 970 980 990 1000
SQDSAFSQIS EHSVFTPTET SSPIDCTFRA QRKQEELSSD CDSPSLVSGM
1010 1020 1030 1040 1050
PGPSTGQASS HLAYLRKGTT EQLPQMHSVT LHPSTWLRSG LVTLKNWSLK
1060 1070 1080 1090 1100
KKTKAARPED RKDCSLKEPL ELPACAAGTP EADSLQESQE DIHLGVDEGA
1110 1120 1130 1140 1150
GQTACGLSSY ACQDSEQHAS SPFCLAGSRL TLGMKLHEGE ESGGQYPCDN
1160 1170 1180
PWEGAPSGLE TSEDAANTGV EPATVCNDGD RH
Length:1,182
Mass (Da):131,138
Last modified:April 3, 2007 - v2
Checksum:i6D3EB56A11EB023E
GO
Isoform 2 (identifier: Q6REY9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Show »
Length:1,146
Mass (Da):127,524
Checksum:iB2261E8D0EC63AE8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti284S → F in AAS77204 (PubMed:15234003).Curated1
Sequence conflicti615S → P in AAS77204 (PubMed:15234003).Curated1
Sequence conflicti637 – 638NF → SL in AAS77204 (PubMed:15234003).Curated2
Sequence conflicti788I → N in AAS77204 (PubMed:15234003).Curated1
Sequence conflicti960S → C in AAS77204 (PubMed:15234003).Curated1
Sequence conflicti1005T → M in AAS77204 (PubMed:15234003).Curated1
Sequence conflicti1099G → E in AAS77204 (PubMed:15234003).Curated1
Sequence conflicti1104A → T in AAS77204 (PubMed:15234003).Curated1
Sequence conflicti1134M → I in AAS77204 (PubMed:15234003).Curated1
Sequence conflicti1156P → T in AAS77204 (PubMed:15234003).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0243021 – 36Missing in isoform 2. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY501475 mRNA. Translation: AAS77204.1.
AABR03065952 Genomic DNA. No translation available.
AABR03062969 Genomic DNA. No translation available.
AABR03064628 Genomic DNA. No translation available.
AABR03062444 Genomic DNA. No translation available.
RefSeqiNP_998794.1. NM_213629.1.
XP_006243109.1. XM_006243047.3. [Q6REY9-1]
UniGeneiRn.20805.

Genome annotation databases

EnsembliENSRNOT00000035989; ENSRNOP00000034071; ENSRNOG00000025624. [Q6REY9-1]
ENSRNOT00000059078; ENSRNOP00000055856; ENSRNOG00000025624. [Q6REY9-2]
GeneIDi367085.
KEGGirno:367085.
UCSCiRGD:1303160. rat. [Q6REY9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY501475 mRNA. Translation: AAS77204.1.
AABR03065952 Genomic DNA. No translation available.
AABR03062969 Genomic DNA. No translation available.
AABR03064628 Genomic DNA. No translation available.
AABR03062444 Genomic DNA. No translation available.
RefSeqiNP_998794.1. NM_213629.1.
XP_006243109.1. XM_006243047.3. [Q6REY9-1]
UniGeneiRn.20805.

3D structure databases

ProteinModelPortaliQ6REY9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034071.

PTM databases

iPTMnetiQ6REY9.
PhosphoSitePlusiQ6REY9.

Proteomic databases

PaxDbiQ6REY9.
PRIDEiQ6REY9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000035989; ENSRNOP00000034071; ENSRNOG00000025624. [Q6REY9-1]
ENSRNOT00000059078; ENSRNOP00000055856; ENSRNOG00000025624. [Q6REY9-2]
GeneIDi367085.
KEGGirno:367085.
UCSCiRGD:1303160. rat. [Q6REY9-1]

Organism-specific databases

CTDi57569.
RGDi1303160. Arhgap20.

Phylogenomic databases

eggNOGiKOG4724. Eukaryota.
ENOG410YV4E. LUCA.
GeneTreeiENSGT00530000063299.
HOGENOMiHOG000048702.
HOVERGENiHBG061748.
InParanoidiQ6REY9.
KOiK20641.
OMAiEQLPREM.
OrthoDBiEOG091G02C4.
PhylomeDBiQ6REY9.
TreeFamiTF331062.

Enzyme and pathway databases

ReactomeiR-RNO-194840. Rho GTPase cycle.

Miscellaneous databases

PROiQ6REY9.

Gene expression databases

BgeeiENSRNOG00000025624.
GenevisibleiQ6REY9. RN.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHG20_RAT
AccessioniPrimary (citable) accession number: Q6REY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: November 2, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.