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Protein

Rho GTPase-activating protein 20

Gene

Arhgap20

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB-KW
  • Rho GTPase binding Source: RGD

GO - Biological processi

  • regulation of Rho protein signal transduction Source: RGD
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-RNO-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 20
Alternative name(s):
RA and RhoGAP domain-containing protein
Short name:
RARhoGAP
Rho-type GTPase-activating protein 20
Gene namesi
Name:Arhgap20
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1303160. Arhgap20.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11821182Rho GTPase-activating protein 20PRO_0000283088Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461PhosphoserineCombined sources
Modified residuei704 – 7041PhosphoserineBy similarity
Modified residuei730 – 7301PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6REY9.
PRIDEiQ6REY9.

PTM databases

iPTMnetiQ6REY9.

Expressioni

Tissue specificityi

Highest expression is found in testis. Ubiquitously expressed in extragonadal tissues.1 Publication

Developmental stagei

Found to be expressed in developing spermatocytes, but not in terminally differentiated spermatozoa.1 Publication

Gene expression databases

GenevisibleiQ6REY9. RN.

Interactioni

GO - Molecular functioni

  • Rho GTPase binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034071.

Structurei

3D structure databases

ProteinModelPortaliQ6REY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 187102PHAdd
BLAST
Domaini194 – 28390Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini365 – 551187Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi910 – 1010101Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.Curated
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4724. Eukaryota.
ENOG410YV4E. LUCA.
GeneTreeiENSGT00530000063299.
HOGENOMiHOG000048702.
HOVERGENiHBG061748.
InParanoidiQ6REY9.
OMAiEQLPREM.
OrthoDBiEOG7NCV2Q.
PhylomeDBiQ6REY9.
TreeFamiTF331062.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6REY9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAMSPQQDA LGAQPGRSSS LTGMSRIAGG PGTKKKMKTL AERRRSAPSL
60 70 80 90 100
ILDKALQKRP STRDSHSASI DTCAFLSSFM CSSRTLLIDG PVELKRGLQR
110 120 130 140 150
QERHLFLFND LFVSAKIKYN NNFKIKNKVK LADMWTASCV DEVGEGNTNA
160 170 180 190 200
LKSFVLGWPT VNFVATFSSP EQKDKWLSLL QRYIALEKEK DYPKSIPLKI
210 220 230 240 250
FAKDIGNCAY FKTISVMNSD TASEVINMSL QMLGITGSER DYQLWVNSGK
260 270 280 290 300
EAAPYPLIGH EYPYGIKMSH LRDTALLTQG SKDSASPSQL QEPFLMEQLP
310 320 330 340 350
REMQCQFILK PTRLATAQQL SDSSHKTYKR RRSIINWAFW RGSSTHLDNL
360 370 380 390 400
PVSPTSPMPG QLFGVSLPDI CENDNLPKPI LDMLSFLNQK GPLTKGIFRQ
410 420 430 440 450
SANMKSCREL KEKLNSGIEV HLDCESIFVI ASVLKDFLRN IPESIFSSDL
460 470 480 490 500
YDHWVCVMDQ GNDEEKINTI QRLLDQLPRA NVVFLRYLFG VLHNIEQHSL
510 520 530 540 550
SNQMTAFNLA VCIAPSILWP PASSSPELEN EFTKKVSLLI QFLIENCCRI
560 570 580 590 600
FGEEITSLLG ELSERSDREH TPDTSCFQLN DSSYDSLENE LNEDADAPCS
610 620 630 640 650
DLVKRLGQGS RSMDSVLTLS DYDLEPPEAE GLLTLSNFDL DQSKEEHIRT
660 670 680 690 700
KQPLETKPVS VFVAYRKVSL GEHTRAPDGP GTPSCLPATA SDARKVFRRH
710 720 730 740 750
RRSSEPSIDY LDAKLSYLRE FYQKKLRKSS CDAVLSRKDE DYLKQTQPQK
760 770 780 790 800
KGDQRCFKQS SVTGTDVSKR NTANENIKKK SLSGHEGIQE TPFTKSKPVA
810 820 830 840 850
ISVASYMSSQ DHSWEQPFEA DACRFSPPHI ADAQKSSRAH RRCSEPSIDD
860 870 880 890 900
QNYKLSYLRG VYSKKQSKTS CEAGLLHGEE DYLKRHKSLQ MEGQKLINQS
910 920 930 940 950
LVMGIEVGKS SSSTNQSTEK VLPPRLNLCP RASYSSLSSP GTSPSGSSVS
960 970 980 990 1000
SQDSAFSQIS EHSVFTPTET SSPIDCTFRA QRKQEELSSD CDSPSLVSGM
1010 1020 1030 1040 1050
PGPSTGQASS HLAYLRKGTT EQLPQMHSVT LHPSTWLRSG LVTLKNWSLK
1060 1070 1080 1090 1100
KKTKAARPED RKDCSLKEPL ELPACAAGTP EADSLQESQE DIHLGVDEGA
1110 1120 1130 1140 1150
GQTACGLSSY ACQDSEQHAS SPFCLAGSRL TLGMKLHEGE ESGGQYPCDN
1160 1170 1180
PWEGAPSGLE TSEDAANTGV EPATVCNDGD RH
Length:1,182
Mass (Da):131,138
Last modified:April 3, 2007 - v2
Checksum:i6D3EB56A11EB023E
GO
Isoform 2 (identifier: Q6REY9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Show »
Length:1,146
Mass (Da):127,524
Checksum:iB2261E8D0EC63AE8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti284 – 2841S → F in AAS77204 (PubMed:15234003).Curated
Sequence conflicti615 – 6151S → P in AAS77204 (PubMed:15234003).Curated
Sequence conflicti637 – 6382NF → SL in AAS77204 (PubMed:15234003).Curated
Sequence conflicti788 – 7881I → N in AAS77204 (PubMed:15234003).Curated
Sequence conflicti960 – 9601S → C in AAS77204 (PubMed:15234003).Curated
Sequence conflicti1005 – 10051T → M in AAS77204 (PubMed:15234003).Curated
Sequence conflicti1099 – 10991G → E in AAS77204 (PubMed:15234003).Curated
Sequence conflicti1104 – 11041A → T in AAS77204 (PubMed:15234003).Curated
Sequence conflicti1134 – 11341M → I in AAS77204 (PubMed:15234003).Curated
Sequence conflicti1156 – 11561P → T in AAS77204 (PubMed:15234003).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3636Missing in isoform 2. 1 PublicationVSP_024302Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY501475 mRNA. Translation: AAS77204.1.
AABR03065952 Genomic DNA. No translation available.
AABR03062969 Genomic DNA. No translation available.
AABR03064628 Genomic DNA. No translation available.
AABR03062444 Genomic DNA. No translation available.
RefSeqiNP_998794.1. NM_213629.1.
XP_006243109.1. XM_006243047.2. [Q6REY9-1]
UniGeneiRn.20805.

Genome annotation databases

EnsembliENSRNOT00000035989; ENSRNOP00000034071; ENSRNOG00000025624. [Q6REY9-1]
ENSRNOT00000059078; ENSRNOP00000055856; ENSRNOG00000025624. [Q6REY9-2]
GeneIDi367085.
KEGGirno:367085.
UCSCiRGD:1303160. rat. [Q6REY9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY501475 mRNA. Translation: AAS77204.1.
AABR03065952 Genomic DNA. No translation available.
AABR03062969 Genomic DNA. No translation available.
AABR03064628 Genomic DNA. No translation available.
AABR03062444 Genomic DNA. No translation available.
RefSeqiNP_998794.1. NM_213629.1.
XP_006243109.1. XM_006243047.2. [Q6REY9-1]
UniGeneiRn.20805.

3D structure databases

ProteinModelPortaliQ6REY9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034071.

PTM databases

iPTMnetiQ6REY9.

Proteomic databases

PaxDbiQ6REY9.
PRIDEiQ6REY9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000035989; ENSRNOP00000034071; ENSRNOG00000025624. [Q6REY9-1]
ENSRNOT00000059078; ENSRNOP00000055856; ENSRNOG00000025624. [Q6REY9-2]
GeneIDi367085.
KEGGirno:367085.
UCSCiRGD:1303160. rat. [Q6REY9-1]

Organism-specific databases

CTDi57569.
RGDi1303160. Arhgap20.

Phylogenomic databases

eggNOGiKOG4724. Eukaryota.
ENOG410YV4E. LUCA.
GeneTreeiENSGT00530000063299.
HOGENOMiHOG000048702.
HOVERGENiHBG061748.
InParanoidiQ6REY9.
OMAiEQLPREM.
OrthoDBiEOG7NCV2Q.
PhylomeDBiQ6REY9.
TreeFamiTF331062.

Enzyme and pathway databases

ReactomeiR-RNO-194840. Rho GTPase cycle.

Miscellaneous databases

PROiQ6REY9.

Gene expression databases

GenevisibleiQ6REY9. RN.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells."
    Curry B.J., Su H., Law E.G., McLaughlin E.A., Nixon B., Aitken R.J.
    Genomics 84:406-418(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Wistar.
    Tissue: Testis.
  2. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRHG20_RAT
AccessioniPrimary (citable) accession number: Q6REY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: June 8, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.