Q6REU8 (TYSY_XENNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	XNC1_4048

Organism

Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6) [Complete proteome] [HAMAP]

Taxonomic identifier

406817 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Xenorhabdus › Xenorhabdus nematophilus

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000141048
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6REU8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2A7C5C0336794B07	FASTA	264	30,352
	10 20 30 40 50 60 MKQYLDLMKK VLEEGTAKDD RTGTGTRSIF GHQMRFNLQE GFPLVTTKRC HLRSIIHELL 70 80 90 100 110 120 WFLNGDTNIK YLHDNGVSIW DEWADENGDL GPVYGKQWRA WGSADGRHID QLTQVLEQLK 130 140 150 160 170 180 GDPDSRRIIV SAWNVGELDK MALAPCHAFF QFYVADGKLS CQLYQRSCDV FLGLPFNIAS 190 200 210 220 230 240 YALLVHMMAQ QCNLDVGDFV WTGGDTHLYN NHLEQTHLQL SREPRALPKL VIKRKPDSLF 250 260 DYRFEDFEIV DYDPYPGIKA PVAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of dTMP synthesis in Xenorhabdus nematophila and evaluation of dT availability in the insect and nematode hosts." Orchard S.S., Goodrich-Blair H. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6.
[2]	"Complete genome sequence of Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6)." Goodrich-Blair H., Barbazuk B., Bode H.B., Darby C., Du Z., Forst S., Gaudriault S., Goldman B.S., Goodner B., Henkhaus J., Latreille P., Medigue C., Miller N., Norton S., Ogier J.C., Rouy Z., Slater S., Suen G. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY502024 Genomic DNA. Translation: AAS80323.1. FN667742 Genomic DNA. Translation: CBJ92073.1.
RefSeq	YP_003714161.1. NC_014228.1.
3D structure databases
ProteinModelPortal	Q6REU8.
SMR	Q6REU8. Positions 1-264.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBJ92073; CBJ92073; XNC1_4048.
GeneID	9431860.
KEGG	xne:XNC1_4048.
PATRIC	42467566. VBIXenNem38452_3714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000257899.
KO	K00560.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_XENNA

Accession

Primary (citable) accession number: Q6REU8
Secondary accession number(s): D3VCN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program