Reviewed, UniProtKB/Swiss-Prot Q6RET7 (CCAMK_MEDTR)

Names and origin

Protein names

Recommended name:
    Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3
    EC=2.7.11.17
Alternative name(s):
    Does not make infections protein 3
    CCaMK DMI3
    MtCCaMK

Gene names

Name:

DMI3

Organism

Medicago truncatula (Barrel medic)

Taxonomic identifier

3880 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Protein kinase that recognizes the calcium spiking induced by Nod factors and translates this signal to components controlling nodulation and mycorrhizal infection responses. May phosphorylate the NSP1 protein.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	High expression in roots, low in flowers. Not detected in leaves or stems. Ref.1
Induction	Small up-regulation in nodules. Ref.1
Post-translational modification	Autophosphorylation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 3 EF-hand domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Nodulation
Cellular component	Membrane
Domain	Coiled coil Repeat Transmembrane
Ligand	ATP-binding Calcium Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	nodulation Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3		PRO_0000085699
Regions
	Transmembrane	231 – 247	17	Potential
	Domain	12 – 306	295	Protein kinase
	Domain	400 – 435	36	EF-hand 1
	Domain	436 – 471	36	EF-hand 2
	Domain	478 – 513	36	EF-hand 3
	Nucleotide binding	18 – 26	9	ATP By similarity
	Calcium binding	413 – 424	12	1 Potential
	Calcium binding	449 – 460	12	2 Potential
	Calcium binding	491 – 502	12	3 Potential
	Region	329 – 342	14	Calmodulin-binding By similarity
	Coiled coil	350 – 384	35	Potential
Sites
	Active site	171	1	Proton acceptor By similarity
	Binding site	47	1	ATP By similarity
Amino acid modifications
	Modified residue	271	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6RET7-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C53517F0A97BE67D	FASTA	523	58,637
	10 20 30 40 50 60 MGYGTRKLSD EYEVSEILGR GGFSVVRKGT KKSSIEEEKS QSQVAIKTLR RLGASNNPSG 70 80 90 100 110 120 LPRKKDIGEK STIGFPTMRQ VSVSDTLLTN EILVMRRIVE NVSPHPNVID LYDVYEDTNG 130 140 150 160 170 180 VHLVLELCSG GELFDRIVAQ DKYSETEAAT VVHQIASGLE AVHRANIVHR DLKPENCLFL 190 200 210 220 230 240 DVRKDSPLKI MDFGLSSVEE FTDPVVGLFG SIDYVSPEAL SQGKITTKSD MWSLGVILYI 250 260 270 280 290 300 LLSGYPPFIA QNNRQKQQMI MNGNFSFYEK TWKGISQPAK NLISSLLTVD PSKRPSALEL 310 320 330 340 350 360 LSDPWVKGEK AKDVQMDPEI VSRLQSFNAR RKLRAAAIAS VWSSTIFLRT KKLKSLVGSY 370 380 390 400 410 420 DLKEEEIENL RMHFKKICAD RDNATLSEFE EVLKAMNMLS LIPFASRIFD LFDNNRDGTV 430 440 450 460 470 480 DMREILCGFS SLKNSKGEDA LRLCFQMYDT DRSGCISKEE VASMLRALPY DCLPTDITEP 490 500 510 520 GKLDEIFDLM DANNDGKVTF DEFKAAMQRD SSLQDVVLSS IRP

References

[1]

"A putative Ca2+ and calmodulin-dependent protein kinase required for bacterial and fungal symbioses." Levy J., Bres C., Geurts R., Chalhoub B., Kulikova O., Duc G., Journet E.-P., Ane J.-M., Lauber E., Bisseling T., Denarie J., Rosenberg C., Debelle F. Science 303:1361-1364(2004) [PubMed: 14963335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Jemalong A17.

[2]

"A Ca2+/calmodulin-dependent protein kinase required for symbiotic nodule development: gene identification by transcript-based cloning." Mitra R.M., Gleason C.A., Edwards A., Hadfield J., Downie J.A., Oldroyd G.E.D., Long S.R. Proc. Natl. Acad. Sci. U.S.A. 101:4701-4705(2004) [PubMed: 15070781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION. Strain: cv. Jemalong A17.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY502066 Genomic DNA. Translation: AAS55541.1. AY496049 mRNA. Translation: AAS75146.1.
3D structure databases
SMR	Q6RET7. Positions 5-336, 260-507, 361-521.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.17. 266820.
Family and domain databases
InterPro	IPR020642. Ca-dep_prot_kinase. IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PANTHER	PTHR22982:SF13. Ca-dep_prot_kinase. 1 hit. PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00054. EFh. 3 hits. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 3 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCAMK_MEDTR

Accession

Primary (citable) accession number: Q6RET7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)