Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q6RET7 (CCAMK_MEDTR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3
EC=2.7.11.17
Alternative name(s):
CCaMK DMI3
Does not make infections protein 3
MtCCaMK
Gene names
Name:DMI3
OrganismMedicago truncatula (Barrel medic) (Medicago tribuloides)
Taxonomic identifier3880 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein kinase that recognizes the calcium spiking induced by Nod factors and translates this signal to components controlling nodulation and mycorrhizal infection responses. May phosphorylate the NSP1 protein.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Tissue specificity

High expression in roots, low in flowers. Not detected in leaves or stems. Ref.1

Induction

Small up-regulation in nodules. Ref.1

Post-translational modification

Autophosphorylation By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Contains 3 EF-hand domains.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 523523Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3
PRO_0000085699

Regions

Transmembrane231 – 24717Helical; Potential
Domain12 – 306295Protein kinase
Domain400 – 43536EF-hand 1
Domain436 – 47136EF-hand 2
Domain478 – 51336EF-hand 3
Nucleotide binding18 – 269ATP By similarity
Calcium binding413 – 424121 Potential
Calcium binding449 – 460122 Potential
Calcium binding491 – 502123 Potential
Region329 – 34214Calmodulin-binding By similarity
Coiled coil350 – 38435 Potential

Sites

Active site1711Proton acceptor By similarity
Binding site471ATP By similarity

Amino acid modifications

Modified residue2711Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6RET7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C53517F0A97BE67D

FASTA52358,637
        10         20         30         40         50         60 
MGYGTRKLSD EYEVSEILGR GGFSVVRKGT KKSSIEEEKS QSQVAIKTLR RLGASNNPSG 

        70         80         90        100        110        120 
LPRKKDIGEK STIGFPTMRQ VSVSDTLLTN EILVMRRIVE NVSPHPNVID LYDVYEDTNG 

       130        140        150        160        170        180 
VHLVLELCSG GELFDRIVAQ DKYSETEAAT VVHQIASGLE AVHRANIVHR DLKPENCLFL 

       190        200        210        220        230        240 
DVRKDSPLKI MDFGLSSVEE FTDPVVGLFG SIDYVSPEAL SQGKITTKSD MWSLGVILYI 

       250        260        270        280        290        300 
LLSGYPPFIA QNNRQKQQMI MNGNFSFYEK TWKGISQPAK NLISSLLTVD PSKRPSALEL 

       310        320        330        340        350        360 
LSDPWVKGEK AKDVQMDPEI VSRLQSFNAR RKLRAAAIAS VWSSTIFLRT KKLKSLVGSY 

       370        380        390        400        410        420 
DLKEEEIENL RMHFKKICAD RDNATLSEFE EVLKAMNMLS LIPFASRIFD LFDNNRDGTV 

       430        440        450        460        470        480 
DMREILCGFS SLKNSKGEDA LRLCFQMYDT DRSGCISKEE VASMLRALPY DCLPTDITEP 

       490        500        510        520 
GKLDEIFDLM DANNDGKVTF DEFKAAMQRD SSLQDVVLSS IRP

« Hide

References

[1]"A putative Ca2+ and calmodulin-dependent protein kinase required for bacterial and fungal symbioses."
Levy J., Bres C., Geurts R., Chalhoub B., Kulikova O., Duc G., Journet E.-P., Ane J.-M., Lauber E., Bisseling T., Denarie J., Rosenberg C., Debelle F.
Science 303:1361-1364(2004) [PubMed: 14963335] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Jemalong A17.
[2]"A Ca2+/calmodulin-dependent protein kinase required for symbiotic nodule development: gene identification by transcript-based cloning."
Mitra R.M., Gleason C.A., Edwards A., Hadfield J., Downie J.A., Oldroyd G.E.D., Long S.R.
Proc. Natl. Acad. Sci. U.S.A. 101:4701-4705(2004) [PubMed: 15070781] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION.
Strain: cv. Jemalong A17.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY502066 Genomic DNA. Translation: AAS55541.1.
AY496049 mRNA. Translation: AAS75146.1.
RefSeqXP_003628124.1. XM_003628076.1.

3D structure databases

ProteinModelPortalQ6RET7.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID11405240.

Family and domain databases

InterProIPR018248. EF-hand.
IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca-bd.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_kinase-like_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR002290. Ser/Thr_kinase_dom.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 3 hits.
PfamPF00036. efhand. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 3 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCCAMK_MEDTR
AccessionPrimary (citable) accession number: Q6RET7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents