Reviewed, UniProtKB/Swiss-Prot Q6RET6 (CCAMK_PEA)

Names and origin

Protein names

Recommended name:
    Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase
    EC=2.7.11.17
Alternative name(s):
    PsCCaMK
    Ps-SYM9

Gene names

Name:

SYM9

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	527 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Protein kinase that recognizes the calcium spiking induced by Nod factors and translates this signal to components controlling nodulation and mycorrhizal infection responses.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Post-translational modification	Autophosphorylation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 3 EF-hand domains. Contains 1 protein kinase domain.
Sequence caution	The sequence AAS55542.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAS55543.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAS55544.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Nodulation
Cellular component	Membrane
Domain	Coiled coil Repeat Transmembrane
Ligand	ATP-binding Calcium Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	nodulation Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›527	›527	Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase		PRO_0000085700
Regions
	Transmembrane	239 – 255	17	Potential
	Domain	12 – 314	303	Protein kinase
	Domain	408 – 443	36	EF-hand 1
	Domain	444 – 479	36	EF-hand 2
	Domain	486 – 521	36	EF-hand 3
	Nucleotide binding	18 – 26	9	ATP By similarity
	Calcium binding	421 – 432	12	1 Potential
	Calcium binding	457 – 468	12	2 Potential
	Calcium binding	499 – 510	12	3 Potential
	Region	337 – 350	14	Calmodulin-binding By similarity
	Coiled coil	358 – 379	22	Potential
Sites
	Active site	179	1	Proton acceptor By similarity
	Binding site	55	1	ATP By similarity
Amino acid modifications
	Modified residue	279	1	Phosphothreonine By similarity
Experimental info
	Mutagenesis	24	1	S → F in DK22; loss of nodulation and mycorrhizal infection. Ref.1
	Mutagenesis	202	1	G → R in DK9; loss of nodulation and mycorrhizal infection. Ref.1
	Non-terminal residue	527	1

Sequences

	Sequence		Length	Mass (Da)
	Q6RET6-1 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 55662E42C62EF462	FASTA	527	59,227
	10 20 30 40 50 60 MEYGTRKLSD VYEVSEILGR GGFSVVRKGT RKSNNDDEKS QSQSKSQSQS QVAIKTLRRL 70 80 90 100 110 120 GTSNNLPRKK DGGENSTETM MKFPTMRQVS VSDALLTNEI LVMRRIVENV SPHPNVIDLY 130 140 150 160 170 180 DVYEDTNGVH LVLELCSGGE LFDRIVAQDK YSETEASTVV HQIVAGLEAI HRANIIHRDL 190 200 210 220 230 240 KPENCLFLDV GKDSSLKIMD FGLSSVEEFT DPVVGLFGSI DYVSPEALSQ GKITTKSDMW 250 260 270 280 290 300 SLGVILYILL SGYPPFIAQN NRQKQQMILN GNFSFYEKTW KGISQSAKNL ISSLLTVDPA 310 320 330 340 350 360 KRPSAQELLS DPWVKGEKAK DDQMDPEIVS RLQRFNARRK LRAAAIASVW SSTIFLRTKK 370 380 390 400 410 420 LKSLVGSYDL KEDEIENLRM HFKKICADRD NATLCEFEEV LKAMKMPSLI PFAARIFDLF 430 440 450 460 470 480 DNNRDGTVDM REILCGFSSL KNSKGEDALR LCFQMYDTDR SGCITKEEVA SMLRALPYDC 490 500 510 520 LPTDITEPGK LDEIFDLMDA NSDGKVTFDE FKAAMQRDSS LQDVVLS

References

[1]

"A putative Ca2+ and calmodulin-dependent protein kinase required for bacterial and fungal symbioses." Levy J., Bres C., Geurts R., Chalhoub B., Kulikova O., Duc G., Journet E.-P., Ane J.-M., Lauber E., Bisseling T., Denarie J., Rosenberg C., Debelle F. Science 303:1361-1364(2004) [PubMed: 14963335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF SER-24 AND GLY-202. Strain: cv. Frisson.

[2]

"A Ca2+/calmodulin-dependent protein kinase required for symbiotic nodule development: gene identification by transcript-based cloning." Mitra R.M., Gleason C.A., Edwards A., Hadfield J., Downie J.A., Oldroyd G.E.D., Long S.R. Proc. Natl. Acad. Sci. U.S.A. 101:4701-4705(2004) [PubMed: 15070781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-465. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY502067 Genomic DNA. Translation: AAS55542.1. Sequence problems. AY502069 Genomic DNA. Translation: AAS55544.1. Sequence problems. AY502068 Genomic DNA. Translation: AAS55543.1. Sequence problems. AJ621916 mRNA. Translation: CAF21911.1.
3D structure databases
HSSP	HSSP built from PDB template 1A06 based on UniProtKB Q63450.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.17. 287.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR018248. EF_hand. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 3 hits. PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000012. EF-hand. 1 hit. PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00054. EFh. 3 hits. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 3 hits. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCAMK_PEA

Accession

Primary (citable) accession number: Q6RET6
Secondary accession number(s): Q6RET4, Q703H2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	October 13, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)