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Protein

Methyl farnesoate epoxidase

Gene

CYP15A1

Organism
Diploptera punctata (Pacific beetle cockroach)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methyl farnesoate to juvenile hormone III acid (methyl (2E,6E)-(10R)-10,11-epoxy-3,7,11-trimethyl-2,6-dodecadienoate) in juvenile hormone biosynthesis.1 Publication

Miscellaneous

The enzyme is present in all insects, except in lepidoptera (moths and butterflies), and is specific for methyl farnesoate. Lepidoptera contain the farnesoate epoxidase, which is specific for farnesoate.Curated

Catalytic activityi

Methyl (2E,6E)-farnesoate + NADPH + O2 = juvenile hormone III + NADP+ + H2O.1 Publication

Cofactori

hemeBy similarity

Enzyme regulationi

Inhibited by substituted imidazole inhibitors TH27, KK96 and KK71.1 Publication

Kineticsi

  1. KM=380 µM for methyl (2E,6E)-farnesoate (with purified enzyme)1 Publication
  2. KM=26 µM for methyl (2E,6E)-farnesoate (with recombinant enzyme expressed in E.coli)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi438Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    GO - Biological processi

    • juvenile hormone biosynthetic process Source: UniProtKB

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    LigandHeme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methyl farnesoate epoxidaseCurated (EC:1.14.13.2021 Publication)
    Alternative name(s):
    Cytochrome P450 CYP15A11 Publication
    Gene namesi
    Name:CYP15A11 Publication
    OrganismiDiploptera punctata (Pacific beetle cockroach)
    Taxonomic identifieri6984 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraPolyneopteraDictyopteraBlattodeaBlaberoideaBlaberidaeDiplopterinaeDiploptera

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 20Sequence analysisAdd BLAST20
    ChainiPRO_000043362021 – 493Methyl farnesoate epoxidaseSequence analysisAdd BLAST473

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi282N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Glycosylationi458N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Specifically expressed in the corpora allata of at the time of maximal juvenile hormone production by the glands.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ6R7M4.
    SMRiQ6R7M4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    KOiK14937.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiView protein in InterPro
    IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR036396. Cyt_P450_sf.
    PfamiView protein in Pfam
    PF00067. p450. 1 hit.
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiView protein in PROSITE
    PS00086. CYTOCHROME_P450. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6R7M4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVIALIVIII FLVCLDVIKP RGYPPGPVWL PVVGSYLWFR REKSRVGYYH
    60 70 80 90 100
    LVWSSLSSRY GPVTGMRLGT DYIVVACGYD AIRDILLRDE FDGRPDGYFF
    110 120 130 140 150
    RLRTFGKRMG VVFTDGPVWQ EQRRFCMQHL RKLGLGSRSM EAHIEEEARD
    160 170 180 190 200
    LVASLHRRSN GGLTAIPMHD VFDICVLNSL WAMLAGHRFD LDDQRLVDLL
    210 220 230 240 250
    DIVHKCFRMI DPSGGLLNQM PPLRFIAPRH SGYTNLMTHL NRIWNFLRET
    260 270 280 290 300
    IDDHRKSFNA DNMRDLIDLF LREMETSKCQ NNSSFEDLQL VSLCLDLFMA
    310 320 330 340 350
    GSETTSNTLG FAVLYMLLYP QVQRRVQDEL DRCVGTDRQP TLQDRRSLRY
    360 370 380 390 400
    LEAVLMEIQR HATIAPSGIP HKALKNTVLM GHTIPKGTTV LVSMWSLHRD
    410 420 430 440 450
    VQHWGDPEVF RPERFISGNG NIKQDDWFMP FGIGKRRCIG ETLAKASLFL
    460 470 480 490
    FFSTLLHNFS ILPSSESPLP SLEGYDGVTL SPKPFSAKLI PRK
    Length:493
    Mass (Da):56,523
    Last modified:July 5, 2004 - v1
    Checksum:i7D6F3D8DD4D171A0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY509244 mRNA. Translation: AAS13464.1.

    Genome annotation databases

    KEGGiag:AAS13464.

    Similar proteinsi

    Entry informationi

    Entry nameiC15A1_DIPPU
    AccessioniPrimary (citable) accession number: Q6R7M4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2015
    Last sequence update: July 5, 2004
    Last modified: October 25, 2017
    This is version 59 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families