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Q6R7H4

- RIR1_OSHVF

UniProt

Q6R7H4 - RIR1_OSHVF

Protein

Ribonucleoside-diphosphate reductase large subunit

Gene
N/A
Organism
Ostreid herpesvirus 1 (isolate France) (OsHV-1) (Pacific oyster herpesvirus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei222 – 2221SubstrateBy similarity
    Sitei238 – 2381Important for hydrogen atom transferBy similarity
    Binding sitei266 – 2661Substrate; via amide nitrogenBy similarity
    Active sitei447 – 4471Proton acceptorBy similarity
    Active sitei449 – 4491Cysteine radical intermediateBy similarity
    Active sitei451 – 4511Proton acceptorBy similarity
    Sitei464 – 4641Important for hydrogen atom transferBy similarity
    Sitei797 – 7971Important for electron transferBy similarity
    Sitei798 – 7981Important for electron transferBy similarity
    Sitei830 – 8301Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei833 – 8331Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
    OrganismiOstreid herpesvirus 1 (isolate France) (OsHV-1) (Pacific oyster herpesvirus)
    Taxonomic identifieri654903 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesMalacoherpesviridaeOstreavirus
    Virus hostiCrassostrea gigas (Pacific oyster) (Crassostrea angulata) [TaxID: 29159]
    Pecten maximus (King scallop) (Pilgrim's clam) [TaxID: 6579]
    ProteomesiUP000007021: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 835835Ribonucleoside-diphosphate reductase large subunitPRO_0000385023Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi238 ↔ 464Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6R7H4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni237 – 2382Substrate bindingBy similarity
    Regioni447 – 4515Substrate bindingBy similarity
    Regioni660 – 6645Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view]
    PfamiPF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    SUPFAMiSSF48168. SSF48168. 1 hit.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6R7H4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MACSMANAVR PGLSDLPEGV DLSNPKEVRS LLIKLTHARK STVYDTKIRR    50
    LHGVIVNSIM ENYNSAEGEL DMAITPLIPS HPDYAFLAGR VSAYMTQINT 100
    PKTFTDAVYG IQMAVGETNG FRSPMKPVKG GLSDEIVDFV NEHGARLDGM 150
    IDDVRDYSHN YMGITGLLNG VAIKVNKKLY ERPQYVFMRV AMAMTDLSSE 200
    TALEDIWQTY MLISGQCLSM ASPVLFNGGT SSSNTASCFL LDMGDNMVSI 250
    TKKVAQTMTL IAKNGGIGIN FSKLRATGSK IGMAGKSSGI GGKLNLFDRV 300
    AQSVDQGENK RPGAIAGYLT DWHADMYDWL YSRYSGSGNE VNKRHVLNMG 350
    LVMSDLFMER VENDKEWSLF SPNDVVGLEE LYGEKFVRAY RAHEANPKIK 400
    RTTVSARKLF SDIANMMWST GEPYILFKDA VNERSNHKHL GTIKNSNLCC 450
    EIVQYCDTNE IAVCNLATIC VSNFVNVETG EIDFEGIADA AGVACKGINN 500
    LIDKQNYDLG NEQLMRLADE RTEEVVEVNG IKFDAKYNLV SYSNLKHRPQ 550
    GIGMQGLHDV FMKLKIPYDS EMAFRLAALI QEAIYYGAVR ESVKIAEEKG 600
    PYPSYYWDNN THREGKLQFD FLEGFDRDRD LTHELFNWRE VLKKFEKFGI 650
    NNSVVTAQPP SASSSQLNDN VESIEAITSN RFTKGIKDGK FIIINKHLQK 700
    DLEDLGMWDA QMVGDIEAND GSIQNIERIP KNIREIYKTA REIDHKAVVK 750
    ICAAIQPFID QTISKNMFVP KNVDNPVQLI MENVILGHKM KLKTGMYYTR 800
    GIPAMKQQNF GKMNFQDKDI GKPVPELVDC EACSA 835
    Length:835
    Mass (Da):93,356
    Last modified:July 5, 2004 - v1
    Checksum:i4C5813635B9E5F47
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY509253 Genomic DNA. Translation: AAS00941.1.
    RefSeqiYP_024594.1. NC_005881.2.

    Genome annotation databases

    GeneIDi2948194.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY509253 Genomic DNA. Translation: AAS00941.1 .
    RefSeqi YP_024594.1. NC_005881.2.

    3D structure databases

    ProteinModelPortali Q6R7H4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2948194.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    InterProi IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view ]
    Pfami PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    SUPFAMi SSF48168. SSF48168. 1 hit.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Tinkering with a viral ribonucleotide reductase."
      Lembo D., Brune W.
      Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiRIR1_OSHVF
    AccessioniPrimary (citable) accession number: Q6R7H4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3