Q6R6M2 (Q6R6M2_BACTK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase HAMAP MF_00091
EC=4.4.1.21 HAMAP MF_00091

Alternative name(s):
AI-2 synthesis protein HAMAP MF_00091
Autoinducer-2 production protein LuxS HAMAP MF_00091

Gene names

Name:	luxS HAMAP MF_00091 EMBL AAR97511.1
ORF Names:	bthur0006_44410 EMBL EEM51190.1

Organism

Bacillus thuringiensis subsp. kurstaki EMBL AAR97511.1

Taxonomic identifier

29339 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. SAAS SAAS003815 HAMAP MF_00091
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. SAAS SAAS003815 HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity. SAAS SAAS003815 HAMAP MF_00091
Subunit structure	Homodimer By similarity. SAAS SAAS003815 HAMAP MF_00091
Sequence similarities	Belongs to the LuxS family. HAMAP MF_00091

Ontologies

Keywords
Biological process	Autoinducer synthesis SAAS SAAS003815 HAMAP MF_00091 Quorum sensing SAAS SAAS003815 HAMAP MF_00091
Ligand	Iron SAAS SAAS003815 HAMAP MF_00091 Metal-binding SAAS SAAS003815 HAMAP MF_00091
Molecular function	Lyase SAAS SAAS003815 HAMAP MF_00091 EMBL EEM51190.1
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	54	1	Iron By similarity HAMAP MF_00091
	Metal binding	58	1	Iron By similarity HAMAP MF_00091
	Metal binding	126	1	Iron By similarity HAMAP MF_00091

Sequences

	Sequence		Length	Mass (Da)
	Q6R6M2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5D2BB8E3B7154793	FASTA	157	17,855
	10 20 30 40 50 60 MPSVESFELD HTIVKAPYVR HCGVHNVGSD GIVNKFDIRF CQPNKQAMKP DVIHTLEHLL 70 80 90 100 110 120 AFNLRKYIDR YPHFDIIDIS PMGCQTGYYL VVSGTPTVRE IIDLLELTLK DAVQITEIPA 130 140 150 ANETQCGQAK LHDLEGAQRL MNFWLSQDKD ELEKVFG

References

[1]

"Annotation of the Bacillus thuringiensis T03a001 genome." Read T.D., Akmal A., Bishop-Lilly K., Chen P.E., Cook C., Kiley M.P., Lentz S., Mateczun A., Nagarajan N., Nolan N., Osborne B.I., Pop M., Sozhamannan S., Stewart A.C., Sulakvelidze A., Thomason B., Willner K., Zwick M.E. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: T03a001 EMBL EEM51190.1.

[2]

"The function of AI-2 in Bacillus thuringiensis." Zhou Y., Sun M., Yu Z., Ye W. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: BMB171 EMBL AAR97510.1, CryB EMBL AAR97512.1 and YBT1520 EMBL AAR97511.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY509886 Genomic DNA. Translation: AAR97510.1. AY509887 Genomic DNA. Translation: AAR97511.1. AY509888 Genomic DNA. Translation: AAR97512.1. ACND01000122 Genomic DNA. Translation: EEM51190.1.
3D structure databases
ProteinModelPortal	Q6R6M2.
SMR	Q6R6M2. Positions 4-157.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000340901; EBBACP00000334119; EBBACG00000340018.
PATRIC	28897637. VBIBacThu4961_1197.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF63411. Metalloenz_metal-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q6R6M2_BACTK

Accession

Primary (citable) accession number: Q6R6M2
Secondary accession number(s): C3ES47

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)