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Protein

Toll-like receptor 13

Gene

Tlr13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of innate and adaptive immunity that recognizes and binds 23S rRNA from bacteria. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Specifically binds the 5'-CGGAAAGACC-3' sequence on bacterial 23S rRNA, a sequence also bound by MLS group antibiotics (including erythromycin). May also recognize vesicular stomatitis virus; however, these data require additional evidences.3 Publications

GO - Molecular functioni

  • double-stranded RNA binding Source: GO_Central
  • protein homodimerization activity Source: MGI
  • rRNA binding Source: UniProtKB

GO - Biological processi

  • defense response to other organism Source: GO_Central
  • inflammatory response Source: UniProtKB-KW
  • innate immune response Source: UniProtKB
  • MyD88-dependent toll-like receptor signaling pathway Source: MGI
  • regulation of MAPK cascade Source: MGI
  • response to virus Source: MGI
  • toll-like receptor 13 signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 13
Gene namesi
Name:Tlr13
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:3045213. Tlr13.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini69 – 783ExtracellularSequence analysisAdd BLAST715
Transmembranei784 – 804HelicalSequence analysisAdd BLAST21
Topological domaini805 – 991CytoplasmicSequence analysisAdd BLAST187

GO - Cellular componenti

  • cytoplasm Source: MGI
  • endosome Source: UniProtKB
  • endosome membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 68Sequence analysisAdd BLAST68
ChainiPRO_000004279569 – 991Toll-like receptor 13Add BLAST923

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi93N-linked (GlcNAc...)Sequence analysis1
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Glycosylationi125N-linked (GlcNAc...)Sequence analysis1
Glycosylationi152N-linked (GlcNAc...)Sequence analysis1
Glycosylationi167N-linked (GlcNAc...)Sequence analysis1
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1
Glycosylationi233N-linked (GlcNAc...)Sequence analysis1
Glycosylationi263N-linked (GlcNAc...)Sequence analysis1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi274N-linked (GlcNAc...)Sequence analysis1
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Glycosylationi357N-linked (GlcNAc...)Sequence analysis1
Glycosylationi388N-linked (GlcNAc...)Sequence analysis1
Glycosylationi413N-linked (GlcNAc...)Sequence analysis1
Glycosylationi421N-linked (GlcNAc...)Sequence analysis1
Glycosylationi644N-linked (GlcNAc...)Sequence analysis1
Glycosylationi663N-linked (GlcNAc...)Sequence analysis1
Glycosylationi711N-linked (GlcNAc...)Sequence analysis1
Glycosylationi742N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ6R5N8.
PaxDbiQ6R5N8.
PeptideAtlasiQ6R5N8.
PRIDEiQ6R5N8.

PTM databases

iPTMnetiQ6R5N8.
PhosphoSitePlusiQ6R5N8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033777.
CleanExiMM_TLR13.
GenevisibleiQ6R5N8. MM.

Interactioni

Subunit structurei

Binds MYD88 via their respective TIR domains (By similarity). Interacts with UNC93B1.By similarity1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

DIPiDIP-61832N.
MINTiMINT-4997493.
STRINGi10090.ENSMUSP00000043101.

Structurei

Secondary structure

1991
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi75 – 77Combined sources3
Beta strandi80 – 85Combined sources6
Helixi94 – 98Combined sources5
Beta strandi107 – 109Combined sources3
Turni120 – 125Combined sources6
Beta strandi131 – 133Combined sources3
Turni144 – 149Combined sources6
Beta strandi155 – 157Combined sources3
Beta strandi178 – 180Combined sources3
Turni191 – 196Combined sources6
Beta strandi202 – 204Combined sources3
Helixi213 – 220Combined sources8
Beta strandi228 – 230Combined sources3
Beta strandi251 – 253Combined sources3
Beta strandi274 – 276Combined sources3
Beta strandi298 – 300Combined sources3
Helixi308 – 310Combined sources3
Helixi313 – 316Combined sources4
Beta strandi321 – 323Combined sources3
Helixi329 – 331Combined sources3
Helixi336 – 343Combined sources8
Beta strandi351 – 353Combined sources3
Helixi361 – 370Combined sources10
Beta strandi374 – 377Combined sources4
Turni391 – 394Combined sources4
Beta strandi399 – 402Combined sources4
Beta strandi424 – 426Combined sources3
Turni437 – 442Combined sources6
Beta strandi448 – 450Combined sources3
Helixi461 – 464Combined sources4
Beta strandi472 – 474Combined sources3
Beta strandi496 – 498Combined sources3
Turni509 – 514Combined sources6
Beta strandi520 – 522Combined sources3
Turni533 – 538Combined sources6
Beta strandi544 – 546Combined sources3
Turni557 – 562Combined sources6
Beta strandi568 – 570Combined sources3
Beta strandi578 – 580Combined sources3
Turni588 – 591Combined sources4
Beta strandi597 – 599Combined sources3
Turni612 – 617Combined sources6
Beta strandi623 – 625Combined sources3
Beta strandi632 – 634Combined sources3
Turni638 – 641Combined sources4
Beta strandi647 – 649Combined sources3
Helixi656 – 659Combined sources4
Beta strandi660 – 662Combined sources3
Turni664 – 669Combined sources6
Beta strandi675 – 677Combined sources3
Beta strandi699 – 701Combined sources3
Turni712 – 717Combined sources6
Beta strandi723 – 725Combined sources3
Helixi735 – 737Combined sources3
Helixi738 – 746Combined sources9
Helixi755 – 757Combined sources3
Helixi769 – 771Combined sources3
Helixi774 – 776Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z0CX-ray2.30A/D69-777[»]
ProteinModelPortaliQ6R5N8.
SMRiQ6R5N8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati104 – 125LRR 1Add BLAST22
Repeati128 – 149LRR 2Add BLAST22
Repeati152 – 174LRR 3Add BLAST23
Repeati175 – 196LRR 4Add BLAST22
Repeati199 – 220LRR 5Add BLAST22
Repeati225 – 246LRR 6Add BLAST22
Repeati248 – 268LRR 7Add BLAST21
Repeati271 – 292LRR 8Add BLAST22
Repeati295 – 315LRR 9Add BLAST21
Repeati318 – 338LRR 10Add BLAST21
Repeati348 – 368LRR 11Add BLAST21
Repeati372 – 394LRR 12Add BLAST23
Repeati397 – 418LRR 13Add BLAST22
Repeati421 – 442LRR 14Add BLAST22
Repeati445 – 466LRR 15Add BLAST22
Repeati469 – 490LRR 16Add BLAST22
Repeati493 – 514LRR 17Add BLAST22
Repeati517 – 538LRR 18Add BLAST22
Repeati541 – 562LRR 19Add BLAST22
Repeati565 – 585LRR 20Add BLAST21
Repeati594 – 617LRR 21Add BLAST24
Repeati620 – 641LRR 22Add BLAST22
Repeati644 – 665LRR 23Add BLAST22
Repeati672 – 693LRR 24Add BLAST22
Repeati696 – 716LRR 25Add BLAST21
Domaini729 – 779LRRCTAdd BLAST51
Domaini832 – 978TIRPROSITE-ProRule annotationAdd BLAST147

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 25 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4641. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000119006.
HOGENOMiHOG000203220.
HOVERGENiHBG094977.
InParanoidiQ6R5N8.
KOiK18808.
OMAiYACPEEC.
OrthoDBiEOG091G018G.
PhylomeDBiQ6R5N8.
TreeFamiTF325595.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 6 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
PF12799. LRR_4. 1 hit.
PF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 18 hits.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 3 hits.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 18 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6R5N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLYRILVQ LEQSPYVKTV PLNMRRDFFF LVVTWMPKTV KMNGSSFVPS
60 70 80 90 100
LQLLLMLVGF SLPPVAETYG FNKCTQYEFD IHHVLCIRKK ITNLTEAISD
110 120 130 140 150
IPRYTTHLNL THNEIQVLPP WSFTNLSALV DLRLEWNSIW KIDEGAFRGL
160 170 180 190 200
ENLTLLNLVE NKIQSVNNSF EGLSSLKTLL LSHNQITHIH KDAFTPLIKL
210 220 230 240 250
KYLSLSRNNI SDFSGILEAV QHLPCLERLD LTNNSIMYLD HSPRSLVSLT
260 270 280 290 300
HLSFEGNKLR ELNFSALSLP NLTNLSASRN GNKVIQNVYL KTLPQLKSLN
310 320 330 340 350
LSGTVIKLEN LSAKHLQNLR AMDLSNWELR HGHLDMKTVC HLLGNLPKLE
360 370 380 390 400
TLVFQKNVTN AEGIKQLAKC TRLLFLDLGQ NSDLIYLNDS EFNALPSLQK
410 420 430 440 450
LNLNKCQLSF INNRTWSSLQ NLTSLDLSHN KFKSFPDFAF SPLKHLEFLS
460 470 480 490 500
LSRNPITELN NLAFSGLFAL KELNLAACWI VTIDRYSFTQ FPNLEVLDLG
510 520 530 540 550
DNNIRTLNHG TFRPLKKLQS LILSHNCLKI LEPNSFSGLT NLRSLDLMYN
560 570 580 590 600
SLSYFHEHLF SGLEKLLILK LGFNKITYET TRTLQYPPFI KLKSLKQLNL
610 620 630 640 650
EGQRHGIQVV PSNFFQGLGS LQELLLGKNP SVFLDHHQFD PLINLTKLDI
660 670 680 690 700
SGTKDGDRSL YLNASLFQNL KRLKILRLEN NNLESLVPDM FSSLQSLQVF
710 720 730 740 750
SLRFNNLKVI NQSHLKNLKS LMFFDVYGNK LQCTCDNLWF KNWSMNTEEV
760 770 780 790 800
HIPFLRSYPC QQPGSQSLLI DFDDAMCNFD LGKVYFLCSF SMVLSTMVFS
810 820 830 840 850
WFSTKMIASL WYGLYICRAW YLTKWHKTEK KFLYDAFVSF SATDEAWVYK
860 870 880 890 900
ELVPALEQGS QTTFKLCLHQ RDFEPGIDIF ENIQNAINTS RKTLCVVSNH
910 920 930 940 950
YLHSEWCRLE VQLASMKMFY EHKDVIILIF LEEIPNYKLS SYHRLRKLIN
960 970 980 990
KQTFITWPDS VHQQPLFWAR IRNALGKETV EKENTHLIVV E
Length:991
Mass (Da):114,443
Last modified:July 5, 2004 - v1
Checksum:i6FB622DC62F37E74
GO

Sequence cautioni

The sequence BAE41528 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY510706 mRNA. Translation: AAS37674.1.
AK170044 mRNA. Translation: BAE41528.1. Different initiation.
AL672288 Genomic DNA. Translation: CAM16892.1.
CH466564 Genomic DNA. Translation: EDL14060.1.
BC117913 mRNA. Translation: AAI17914.1.
BC117914 mRNA. Translation: AAI17915.1.
CCDSiCCDS30338.1.
RefSeqiNP_991389.1. NM_205820.1.
UniGeneiMm.336203.

Genome annotation databases

EnsembliENSMUST00000040065; ENSMUSP00000043101; ENSMUSG00000033777.
GeneIDi279572.
KEGGimmu:279572.
UCSCiuc009ubn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY510706 mRNA. Translation: AAS37674.1.
AK170044 mRNA. Translation: BAE41528.1. Different initiation.
AL672288 Genomic DNA. Translation: CAM16892.1.
CH466564 Genomic DNA. Translation: EDL14060.1.
BC117913 mRNA. Translation: AAI17914.1.
BC117914 mRNA. Translation: AAI17915.1.
CCDSiCCDS30338.1.
RefSeqiNP_991389.1. NM_205820.1.
UniGeneiMm.336203.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z0CX-ray2.30A/D69-777[»]
ProteinModelPortaliQ6R5N8.
SMRiQ6R5N8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61832N.
MINTiMINT-4997493.
STRINGi10090.ENSMUSP00000043101.

PTM databases

iPTMnetiQ6R5N8.
PhosphoSitePlusiQ6R5N8.

Proteomic databases

MaxQBiQ6R5N8.
PaxDbiQ6R5N8.
PeptideAtlasiQ6R5N8.
PRIDEiQ6R5N8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040065; ENSMUSP00000043101; ENSMUSG00000033777.
GeneIDi279572.
KEGGimmu:279572.
UCSCiuc009ubn.1. mouse.

Organism-specific databases

CTDi279572.
MGIiMGI:3045213. Tlr13.

Phylogenomic databases

eggNOGiKOG4641. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000119006.
HOGENOMiHOG000203220.
HOVERGENiHBG094977.
InParanoidiQ6R5N8.
KOiK18808.
OMAiYACPEEC.
OrthoDBiEOG091G018G.
PhylomeDBiQ6R5N8.
TreeFamiTF325595.

Miscellaneous databases

PROiQ6R5N8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033777.
CleanExiMM_TLR13.
GenevisibleiQ6R5N8. MM.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 6 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
PF12799. LRR_4. 1 hit.
PF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 18 hits.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 3 hits.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 18 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLR13_MOUSE
AccessioniPrimary (citable) accession number: Q6R5N8
Secondary accession number(s): Q148Y7, Q3TDS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The sequence 23S rRNA from clinical isolates of erythromycin-resistant S.aureus is methylated and is not recognized by Tlr13 anymore, suggesting a link between antibiotic resistance and evasion from Tlr13 recognition. 23S rRNA modifications generating resistance toward MLS antibiotics preventing recognition of bacteria from Tlr13. These data may also explain why Tlr13 is not conserved in human: human may instead possess a related rRNA-sensing pattern recognition receptor that has evolved to recognize species that can hide from Tlr13 owing to rRNA modifications (PubMed:22821982).1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.