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Protein

E3 ubiquitin-protein ligase RMA1H1

Gene

RMA1H1

Organism
Capsicum annuum (Bell pepper)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase required for aquaporin levels regulation.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri41 – 9050RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • response to cold Source: UniProtKB
  • response to ethylene Source: UniProtKB
  • response to osmotic stress Source: UniProtKB
  • response to water deprivation Source: UniProtKB
  • response to wounding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RMA1H1 (EC:6.3.2.-)
Alternative name(s):
Protein RING membrane-anchor 1 homolog 1
Gene namesi
Name:RMA1H1
OrganismiCapsicum annuum (Bell pepper)
Taxonomic identifieri4072 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeCapsiceaeCapsicum

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Single-pass type IV membrane protein 1 Publication

  • Note: Localization experiments made in a heterologous system.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei232 – 25120Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi58 – 581H → A: Strong reduction of ubiquitin ligase activity. 1 Publication
Mutagenesisi61 – 611C → S: Strong reduction of ubiquitin ligase activity. 1 Publication
Mutagenesisi89 – 891C → S: Strong reduction of ubiquitin ligase activity. 1 Publication
Mutagenesisi115 – 1151K → R: No effect on ubiquitin ligase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252E3 ubiquitin-protein ligase RMA1H1PRO_0000395676Add
BLAST

Expressioni

Inductioni

By drought, cold, high salt, wounding and ethylene. Not induced by abscisic acid.2 Publications

Interactioni

Subunit structurei

Interacts with Arabidopsis PIP2-1.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6R567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-type zinc finger domain is required for E3 ligase activity.

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri41 – 9050RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6R567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQDMALEQL DTTFNKHDTP LGKWKSMNDE VEENISGGFD CNICLDCVHE
60 70 80 90 100
PVITLCGHLY CWPCIYKWIY FQSVSSENSD QQQPQCPVCK AEVSEKTLIP
110 120 130 140 150
LYGRGGQSTK PSEGKAPNLG IVIPQRPPSP RCGGHFLLPT TDSNPSQLLQ
160 170 180 190 200
RRGYQQQSQT RQPAYQGSYM SSPMLSPGGA TANMLQHSMI GEVAYARIFG
210 220 230 240 250
NSSTTMYTYP NSYNLAISSS PRMRRQLSQA DRSLGRICFF LFCCFVTCLI

LF
Length:252
Mass (Da):28,211
Last modified:July 5, 2004 - v1
Checksum:iD05AE7113CEBAE49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY513612 mRNA. Translation: AAR99376.1.
UniGeneiCan.465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY513612 mRNA. Translation: AAR99376.1.
UniGeneiCan.465.

3D structure databases

ProteinModelPortaliQ6R567.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRMA1_CAPAN
AccessioniPrimary (citable) accession number: Q6R567
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.