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Protein

DNA polymerase iota

Gene

Poli

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity).By similarity1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341MagnesiumPROSITE-ProRule annotation
Binding sitei39 – 391dNTPBy similarity
Binding sitei71 – 711dNTPBy similarity
Metal bindingi126 – 1261MagnesiumPROSITE-ProRule annotation
Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  • damaged DNA binding Source: InterPro
  • DNA-directed DNA polymerase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to UV-C Source: MGI
  • DNA replication Source: UniProtKB-KW
  • translesion synthesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Schiff base

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase iota (EC:2.7.7.7)
Alternative name(s):
Rad30 homolog B
Gene namesi
Name:Poli
Synonyms:Rad30b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1347081. Poli.

Subcellular locationi

  • Nucleus By similarity

  • Note: Accumulates at replication forks after DNA damage.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5159.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 717717DNA polymerase iotaPRO_0000173989Add
BLAST

Proteomic databases

EPDiQ6R3M4.
MaxQBiQ6R3M4.
PaxDbiQ6R3M4.
PRIDEiQ6R3M4.

PTM databases

iPTMnetiQ6R3M4.
PhosphoSiteiQ6R3M4.

Expressioni

Tissue specificityi

Detected in testis, and at very low levels in spleen, lung and brain. Detected in round spermatids, but not in prophase spermatocytes.1 Publication

Gene expression databases

BgeeiQ6R3M4.
CleanExiMM_POLI.

Interactioni

Subunit structurei

Interacts with POLH (By similarity). Interacts with REV1.By similarity1 Publication

Protein-protein interaction databases

BioGridi204998. 4 interactions.
IntActiQ6R3M4. 1 interaction.
STRINGi10090.ENSMUSP00000112563.

Chemistry

BindingDBiQ6R3M4.

Structurei

Secondary structure

1
717
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi502 – 5043Combined sources
Turni505 – 5073Combined sources
Helixi510 – 5167Combined sources
Beta strandi682 – 6843Combined sources
Helixi687 – 6904Combined sources
Helixi695 – 7006Combined sources
Helixi701 – 7055Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KWUNMR-A673-717[»]
2KWVNMR-A487-532[»]
3AI4X-ray1.60A668-717[»]
ProteinModelPortaliQ6R3M4.
SMRiQ6R3M4. Positions 27-413, 491-526, 675-710.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 243214UmuCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni300 – 3078DNA bindingBy similarity
Regioni343 – 36018DNA bindingBy similarityAdd
BLAST

Domaini

The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated
Contains 1 umuC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2095. Eukaryota.
COG0389. LUCA.
HOGENOMiHOG000234325.
HOVERGENiHBG053634.
InParanoidiQ6R3M4.
KOiK03510.
OrthoDBiEOG7C8GGJ.
PhylomeDBiQ6R3M4.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6R3M4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPLHAGAAG SSRAVCSQGP PTQISSSRVI VHVDLDCFYA QVEMISNPEL
60 70 80 90 100
KDRPLGVQQK YLVVTCNYEA RKLGVRKLMN VRDAKEKCPQ LVLVNGEDLS
110 120 130 140 150
RYREMSYKVT ELLEEFSPAV ERLGFDENFV DLTEMVEKRL QQLPSEEVPS
160 170 180 190 200
VTVFGHVYNN QSVNLHNIMH RRLVVGSQIA AEMREAMYNQ LGLTGCAGVA
210 220 230 240 250
PNKLLAKLVS GVFKPNQQTV LLPESCQHLI HSLNHIKEIP GIGYKTAKRL
260 270 280 290 300
EVLGINSVHD LQTFPIKTLE KELGIAIAQR IQQLSFGEDK SPVTPSGPPQ
310 320 330 340 350
SFSEEDTFKK CSSEVEAKAK IEELLSSLLT RVCQDGRKPH TVRLVIRRYS
360 370 380 390 400
DKHCNRESRQ CPIPSHVIQK LGTGNHDSMP PLIDILMKLF RNMVNVKMPF
410 420 430 440 450
HLTLMSVCFC NLKALSSAKK GPMDCYLTSL STPAYTDKRA FKVKDTHTED
460 470 480 490 500
SHKEKEANWD CLPSRRIEST GTGESPLDAT CFPKEKDTSD LPLQALPEGV
510 520 530 540 550
DQEVFKQLPA DIQEEILYGK SRENLKGKGS LSCPLHASRG VLSFFSTKQM
560 570 580 590 600
QASRLSPRDT ALPSKRVSAA SPCEPGTSGL SPGSTSHPSC GKDCSYYIDS
610 620 630 640 650
QLKDEQTSQG PTESQGCQFS STNPAVSGFH SFPNLQTEQL FSTHRTVDSH
660 670 680 690 700
KQTATASHQG LESHQGLESR ELDSAEEKLP FPPDIDPQVF YELPEEVQKE
710
LMAEWERAGA ARPSAHR
Length:717
Mass (Da):79,653
Last modified:July 5, 2004 - v1
Checksum:i43E5D2D0ECB3AA85
GO
Isoform 2 (identifier: Q6R3M4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Note: No experimental confirmation available.
Show »
Length:674
Mass (Da):75,156
Checksum:iEFF56FAAD106C994
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti583 – 5831G → R in AAD50424 (PubMed:10458907).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti4 – 41L → S in strain: BALB/c. 2 Publications
Natural varianti7 – 71G → R in strain: BALB/c. 2 Publications
Natural varianti334 – 3341Q → K in strain: BALB/c. 1 Publication
Natural varianti378 – 3781S → A in strain: BALB/c. 1 Publication
Natural varianti518 – 5181Y → S in strain: BALB/c. 3 Publications
Natural varianti524 – 5241N → Y in strain: BALB/c. 1 Publication
Natural varianti569 – 5691A → E in strain: BALB/c. 1 Publication
Natural varianti574 – 5741E → D in strain: BALB/c. 1 Publication
Natural varianti606 – 6061Q → R in strain: BALB/c. 1 Publication
Natural varianti643 – 6431T → I in strain: BALB/c. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4343Missing in isoform 2. 1 PublicationVSP_012800Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151691 mRNA. Translation: AAD50424.1.
AY515316 mRNA. Translation: AAS75834.1.
AY515317 mRNA. Translation: AAS75835.1.
BC082278 mRNA. Translation: AAH82278.1.
CCDSiCCDS50317.1. [Q6R3M4-2]
RefSeqiNP_001129562.1. NM_001136090.2.
NP_001276444.1. NM_001289515.1.
NP_001276445.1. NM_001289516.1.
XP_006526009.1. XM_006525946.2.
UniGeneiMm.244352.

Genome annotation databases

GeneIDi26447.
KEGGimmu:26447.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151691 mRNA. Translation: AAD50424.1.
AY515316 mRNA. Translation: AAS75834.1.
AY515317 mRNA. Translation: AAS75835.1.
BC082278 mRNA. Translation: AAH82278.1.
CCDSiCCDS50317.1. [Q6R3M4-2]
RefSeqiNP_001129562.1. NM_001136090.2.
NP_001276444.1. NM_001289515.1.
NP_001276445.1. NM_001289516.1.
XP_006526009.1. XM_006525946.2.
UniGeneiMm.244352.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KWUNMR-A673-717[»]
2KWVNMR-A487-532[»]
3AI4X-ray1.60A668-717[»]
ProteinModelPortaliQ6R3M4.
SMRiQ6R3M4. Positions 27-413, 491-526, 675-710.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204998. 4 interactions.
IntActiQ6R3M4. 1 interaction.
STRINGi10090.ENSMUSP00000112563.

Chemistry

BindingDBiQ6R3M4.
ChEMBLiCHEMBL5159.

PTM databases

iPTMnetiQ6R3M4.
PhosphoSiteiQ6R3M4.

Proteomic databases

EPDiQ6R3M4.
MaxQBiQ6R3M4.
PaxDbiQ6R3M4.
PRIDEiQ6R3M4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi26447.
KEGGimmu:26447.

Organism-specific databases

CTDi11201.
MGIiMGI:1347081. Poli.

Phylogenomic databases

eggNOGiKOG2095. Eukaryota.
COG0389. LUCA.
HOGENOMiHOG000234325.
HOVERGENiHBG053634.
InParanoidiQ6R3M4.
KOiK03510.
OrthoDBiEOG7C8GGJ.
PhylomeDBiQ6R3M4.

Miscellaneous databases

NextBioi304545.
PROiQ6R3M4.
SOURCEiSearch...

Gene expression databases

BgeeiQ6R3M4.
CleanExiMM_POLI.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta."
    McDonald J.P., Rapic-Otrin V., Epstein J.A., Broughton B.C., Wang X., Lehmann A.R., Wolgemuth D.J., Woodgate R.
    Genomics 60:20-30(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-4; ARG-7 AND SER-518, TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Testis.
  2. "Pol iota is a candidate for the mouse pulmonary adenoma resistance 2 locus, a major modifier of chemically induced lung neoplasia."
    Wang M., Devereux T.R., Vikis H.G., McCulloch S.D., Holliday W., Anna C., Wang Y., Bebenek K., Kunkel T.A., Guan K., You M.
    Cancer Res. 64:1924-1931(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, VARIANTS SER-4; ARG-7; LYS-334; ALA-378; SER-518; TYR-524; GLU-569; ASP-574; ARG-606 AND ILE-643, ALTERNATIVE SPLICING.
    Strain: A/J and BALB/c.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT SER-518.
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. "Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis."
    Guo C., Fischhaber P.L., Luk-Paszyc M.J., Masuda Y., Zhou J., Kamiya K., Kisker C., Friedberg E.C.
    EMBO J. 22:6621-6630(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH REV1.

Entry informationi

Entry nameiPOLI_MOUSE
AccessioniPrimary (citable) accession number: Q6R3M4
Secondary accession number(s): Q641P1, Q6R3M3, Q9R1A6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: July 5, 2004
Last modified: March 16, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.