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Protein

DNA polymerase iota

Gene

Poli

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity).By similarity1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34MagnesiumPROSITE-ProRule annotation1
Binding sitei39dNTPBy similarity1
Binding sitei71dNTPBy similarity1
Metal bindingi126MagnesiumPROSITE-ProRule annotation1
Active sitei127Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • damaged DNA binding Source: InterPro
  • DNA-directed DNA polymerase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to UV-C Source: MGI
  • DNA replication Source: UniProtKB-KW
  • translesion synthesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Schiff base

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase iota (EC:2.7.7.7)
Alternative name(s):
Rad30 homolog B
Gene namesi
Name:Poli
Synonyms:Rad30b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1347081. Poli.

Subcellular locationi

  • Nucleus By similarity

  • Note: Accumulates at replication forks after DNA damage.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5159.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739891 – 717DNA polymerase iotaAdd BLAST717

Proteomic databases

PaxDbiQ6R3M4.
PRIDEiQ6R3M4.

PTM databases

iPTMnetiQ6R3M4.
PhosphoSitePlusiQ6R3M4.

Expressioni

Tissue specificityi

Detected in testis, and at very low levels in spleen, lung and brain. Detected in round spermatids, but not in prophase spermatocytes.1 Publication

Gene expression databases

BgeeiENSMUSG00000038425.
CleanExiMM_POLI.

Interactioni

Subunit structurei

Interacts with POLH (By similarity). Interacts with REV1.By similarity1 Publication

Protein-protein interaction databases

BioGridi204998. 4 interactors.
IntActiQ6R3M4. 1 interactor.
STRINGi10090.ENSMUSP00000112563.

Chemistry databases

BindingDBiQ6R3M4.

Structurei

Secondary structure

1717
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi502 – 504Combined sources3
Turni505 – 507Combined sources3
Helixi510 – 516Combined sources7
Beta strandi682 – 684Combined sources3
Helixi687 – 690Combined sources4
Helixi695 – 700Combined sources6
Helixi701 – 705Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KWUNMR-A673-717[»]
2KWVNMR-A487-532[»]
3AI4X-ray1.60A668-717[»]
ProteinModelPortaliQ6R3M4.
SMRiQ6R3M4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 243UmuCPROSITE-ProRule annotationAdd BLAST214

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni300 – 307DNA bindingBy similarity8
Regioni343 – 360DNA bindingBy similarityAdd BLAST18

Domaini

The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated
Contains 1 umuC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2095. Eukaryota.
COG0389. LUCA.
HOGENOMiHOG000234325.
HOVERGENiHBG053634.
InParanoidiQ6R3M4.
KOiK03510.
PhylomeDBiQ6R3M4.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6R3M4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPLHAGAAG SSRAVCSQGP PTQISSSRVI VHVDLDCFYA QVEMISNPEL
60 70 80 90 100
KDRPLGVQQK YLVVTCNYEA RKLGVRKLMN VRDAKEKCPQ LVLVNGEDLS
110 120 130 140 150
RYREMSYKVT ELLEEFSPAV ERLGFDENFV DLTEMVEKRL QQLPSEEVPS
160 170 180 190 200
VTVFGHVYNN QSVNLHNIMH RRLVVGSQIA AEMREAMYNQ LGLTGCAGVA
210 220 230 240 250
PNKLLAKLVS GVFKPNQQTV LLPESCQHLI HSLNHIKEIP GIGYKTAKRL
260 270 280 290 300
EVLGINSVHD LQTFPIKTLE KELGIAIAQR IQQLSFGEDK SPVTPSGPPQ
310 320 330 340 350
SFSEEDTFKK CSSEVEAKAK IEELLSSLLT RVCQDGRKPH TVRLVIRRYS
360 370 380 390 400
DKHCNRESRQ CPIPSHVIQK LGTGNHDSMP PLIDILMKLF RNMVNVKMPF
410 420 430 440 450
HLTLMSVCFC NLKALSSAKK GPMDCYLTSL STPAYTDKRA FKVKDTHTED
460 470 480 490 500
SHKEKEANWD CLPSRRIEST GTGESPLDAT CFPKEKDTSD LPLQALPEGV
510 520 530 540 550
DQEVFKQLPA DIQEEILYGK SRENLKGKGS LSCPLHASRG VLSFFSTKQM
560 570 580 590 600
QASRLSPRDT ALPSKRVSAA SPCEPGTSGL SPGSTSHPSC GKDCSYYIDS
610 620 630 640 650
QLKDEQTSQG PTESQGCQFS STNPAVSGFH SFPNLQTEQL FSTHRTVDSH
660 670 680 690 700
KQTATASHQG LESHQGLESR ELDSAEEKLP FPPDIDPQVF YELPEEVQKE
710
LMAEWERAGA ARPSAHR
Length:717
Mass (Da):79,653
Last modified:July 5, 2004 - v1
Checksum:i43E5D2D0ECB3AA85
GO
Isoform 2 (identifier: Q6R3M4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Note: No experimental confirmation available.
Show »
Length:674
Mass (Da):75,156
Checksum:iEFF56FAAD106C994
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti583G → R in AAD50424 (PubMed:10458907).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti4L → S in strain: BALB/c. 2 Publications1
Natural varianti7G → R in strain: BALB/c. 2 Publications1
Natural varianti334Q → K in strain: BALB/c. 1 Publication1
Natural varianti378S → A in strain: BALB/c. 1 Publication1
Natural varianti518Y → S in strain: BALB/c. 3 Publications1
Natural varianti524N → Y in strain: BALB/c. 1 Publication1
Natural varianti569A → E in strain: BALB/c. 1 Publication1
Natural varianti574E → D in strain: BALB/c. 1 Publication1
Natural varianti606Q → R in strain: BALB/c. 1 Publication1
Natural varianti643T → I in strain: BALB/c. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0128001 – 43Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151691 mRNA. Translation: AAD50424.1.
AY515316 mRNA. Translation: AAS75834.1.
AY515317 mRNA. Translation: AAS75835.1.
BC082278 mRNA. Translation: AAH82278.1.
CCDSiCCDS50317.1. [Q6R3M4-2]
RefSeqiNP_001129562.1. NM_001136090.2.
NP_001276444.1. NM_001289515.1.
NP_001276445.1. NM_001289516.1.
XP_006526009.1. XM_006525946.3.
UniGeneiMm.244352.

Genome annotation databases

GeneIDi26447.
KEGGimmu:26447.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151691 mRNA. Translation: AAD50424.1.
AY515316 mRNA. Translation: AAS75834.1.
AY515317 mRNA. Translation: AAS75835.1.
BC082278 mRNA. Translation: AAH82278.1.
CCDSiCCDS50317.1. [Q6R3M4-2]
RefSeqiNP_001129562.1. NM_001136090.2.
NP_001276444.1. NM_001289515.1.
NP_001276445.1. NM_001289516.1.
XP_006526009.1. XM_006525946.3.
UniGeneiMm.244352.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KWUNMR-A673-717[»]
2KWVNMR-A487-532[»]
3AI4X-ray1.60A668-717[»]
ProteinModelPortaliQ6R3M4.
SMRiQ6R3M4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204998. 4 interactors.
IntActiQ6R3M4. 1 interactor.
STRINGi10090.ENSMUSP00000112563.

Chemistry databases

BindingDBiQ6R3M4.
ChEMBLiCHEMBL5159.

PTM databases

iPTMnetiQ6R3M4.
PhosphoSitePlusiQ6R3M4.

Proteomic databases

PaxDbiQ6R3M4.
PRIDEiQ6R3M4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi26447.
KEGGimmu:26447.

Organism-specific databases

CTDi11201.
MGIiMGI:1347081. Poli.

Phylogenomic databases

eggNOGiKOG2095. Eukaryota.
COG0389. LUCA.
HOGENOMiHOG000234325.
HOVERGENiHBG053634.
InParanoidiQ6R3M4.
KOiK03510.
PhylomeDBiQ6R3M4.

Miscellaneous databases

PROiQ6R3M4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038425.
CleanExiMM_POLI.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOLI_MOUSE
AccessioniPrimary (citable) accession number: Q6R3M4
Secondary accession number(s): Q641P1, Q6R3M3, Q9R1A6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.