Reviewed,
UniProtKB/Swiss-Prot Q6R327 (RICTR_HUMAN)
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June 16, 2009.
Version 42.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Rapamycin-insensitive companion of mTOR | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1708 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Plays an essential role in embryonic growth and development By similarity. Part of the TORC2 complex which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Ref.1 Ref.6 Ref.2 |
| Subunit structure | Forms part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to FRAP1 and PROTOR1 within the TORC2 complex. May interact with PROTOR2. |
| Sequence similarities | Belongs to the pianissimo family. |
| Sequence caution | The sequence CAH18135.1 differs from that shown. Reason: Frameshift at position 251. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing Polymorphism |
| Molecular function | Developmental protein |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | actin cytoskeleton reorganization Ref.1 Inferred from mutant phenotype. Source: UniProtKB embryonic developmentInferred from sequence or structural similarity. Source: UniProtKB regulation of protein kinase B signaling cascade Ref.6Inferred from direct assay. Source: UniProtKB |
| Cellular component | TORC2 complex Ref.1 Inferred from direct assay. Source: UniProtKB |
| Molecular function | guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| FRAP1 | P42345 | 1 | EBI-1387196,EBI-359260 | |
| ILK | Q13418 | 3 | EBI-1387196,EBI-747644 |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 Ref.1 (identifier: Q6R327-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 Ref.2 (identifier: Q6R327-2) The sequence of this isoform differs from the canonical sequence as follows: 1467-1504: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 3 Ref.2 (identifier: Q6R327-3) The sequence of this isoform differs from the canonical sequence as follows: 1379-1379: R → RIDFKKKHVGGIRSLRPTITNNLFR | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1708 | 1708 | Rapamycin-insensitive companion of mTOR | PRO_0000308179 | |||||
Regions | |||||||||
| Compositional bias | 1016 – 1058 | 43 | Ser-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 21 | 1 | Phosphoserine Ref.7 Ref.10 Ref.11 Ref.12 | ||||||
| Modified residue | 1282 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 1295 | 1 | Phosphothreonine Ref.10 | ||||||
| Modified residue | 1353 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 1386 | 1 | Phosphotyrosine Ref.12 | ||||||
| Modified residue | 1388 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 1396 | 1 | Phosphoserine Ref.11 Ref.12 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1379 | 1 | R → RIDFKKKHVGGIRSLRPTIT NNLFR in isoform 3. Ref.2 | VSP_052581 | |||||
| Alternative sequence | 1467 – 1504 | 38 | Missing in isoform 2. Ref.2 | VSP_052582 | |||||
| Natural variant | 837 | 1 | S → F: dbSNP rs2043112. Ref.2 Ref.4 | VAR_051320 | |||||
Experimental info | |||||||||
| Sequence conflict | 545 | 1 | E → G in CAH18135. Ref.2 | ||||||
| Sequence conflict | 815 | 1 | F → L in CAH18135. Ref.2 | ||||||
| Sequence conflict | 1283 | 1 | N → S in CAH18135. Ref.2 | ||||||
| Sequence conflict | 1699 | 1 | Q → R in AAH51729. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton." Sarbassov D.D., Ali S.M., Kim D.-H., Guertin D.A., Latek R.R., Erdjument-Bromage H., Tempst P., Sabatini D.M. Curr. Biol. 14:1296-1302(2004) [PubMed: 15268862] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, IDENTIFICATION IN TORC2 COMPLEX, INTERACTION WITH FRAP1. |
| [2] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 932-1708 (ISOFORM 3), VARIANT PHE-837. Tissue: Amygdala. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R.  Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PHE-837. Tissue: Brain and Lymph. |
| [5] | "Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method." Ohara O., Nagase T., Mitsui G., Kohga H., Kikuno R., Hiraoka S., Takahashi Y., Kitajima S., Saga Y., Koseki H. DNA Res. 9:47-57(2002) [PubMed: 12056414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 434-1708 (ISOFORM 1). Tissue: Brain. |
| [6] | "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." Sarbassov D.D., Guertin D.A., Ali S.M., Sabatini D.M. Science 307:1098-1101(2005) [PubMed: 15718470] [Abstract] Cited for: FUNCTION. |
| [7] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY. Tissue: Epithelium. |
| [8] | "Identification of Protor as a novel Rictor-binding component of mTOR complex-2." Pearce L.R., Huang X., Boudeau J., Pawlowski R., Wullschleger S., Deak M., Ibrahim A.F.M., Gourlay R., Magnuson M.A., Alessi D.R. Biochem. J. 405:513-522(2007) [PubMed: 17461779] [Abstract] Cited for: IDENTIFICATION IN TORC2 COMPLEX, INTERACTION WITH PROTOR1 AND PROTOR2. |
| [9] | "PRR5, a novel component of mTOR complex 2, regulates platelet-derived growth factor receptor beta expression and signaling." Woo S.-Y., Kim D.-H., Jun C.-B., Kim Y.-M., Haar E.V., Lee S.-I., Hegg J.W., Bandhakavi S., Griffin T.J., Kim D.-H. J. Biol. Chem. 282:25604-25612(2007) [PubMed: 17599906] [Abstract] Cited for: IDENTIFICATION IN TORC2 COMPLEX, INTERACTION WITH PROTOR1. |
| [10] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21 AND THR-1295, MASS SPECTROMETRY. |
| [11] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-1353 AND SER-1396, MASS SPECTROMETRY. |
| [12] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-1282; TYR-1386; SER-1388 AND SER-1396, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AY515854 mRNA. Translation: AAS79796.1. AL834497 mRNA. Translation: CAD39155.1. CR749280 mRNA. Translation: CAH18135.1. Frameshift. CH471119 Genomic DNA. Translation: EAW55980.1. BC051729 mRNA. Translation: AAH51729.1. BC137163 mRNA. Translation: AAI37164.1. BC137164 mRNA. Translation: AAI37165.1. AB082530 mRNA. Translation: BAC02708.1. | |
| IPI | IPI00166528. IPI00455500. IPI00868848. |
| RefSeq | NP_689969.2. |
| UniGene | Hs.407926 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q6R327. 33 interactions. |
PTM databases | |
| PhosphoSite | Q6R327. |
Proteomic databases | |
| PRIDE | Q6R327. |
Genome annotation databases | |
| Ensembl | ENSG00000164327. Homo sapiens. [Contig view] |
| GeneID | 253260. |
| KEGG | hsa:253260. |
Organism-specific databases | |
| GeneCards | GC05M038977. |
| H-InvDB | HIX0021738. |
| MIM | 609022. gene. |
| HUGE | Search... |
Phylogenomic databases | |
| HOGENOM | Q6R327. |
| HOVERGEN | Q6R327. |
| OMA | Q6R327. RFHEMKK. |
Enzyme and pathway databases | |
| Pathway_Interaction_DB | pi3kciaktpathway. Class I PI3K signaling events mediated by Akt. |
Gene expression databases | |
| ArrayExpress | Q6R327. |
| Bgee | Q6R327. |
Family and domain databases | |
| InterPro | IPR000651. RasGef_N. [Graphical view] |
| Pfam | PF00618. RasGEF_N. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 92081. |
| SOURCE | Search... |
Entry information
| Entry name | RICTR_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q6R327 Secondary accession number(s): B2RNX0 Q8NCM6 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |
