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Protein

Endonuclease 8-like 2

Gene

Neil2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).By similarity

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Acetylation of Lys-50 leads to loss of DNA nicking activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNAPROSITE-ProRule annotation1
Active sitei3Proton donorPROSITE-ProRule annotation1
Active sitei50Proton donor; for beta-elimination activityPROSITE-ProRule annotation1
Binding sitei227DNABy similarity1
Active sitei306Proton donor; for delta-elimination activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri280 – 316FPG-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-110329. Cleavage of the damaged pyrimidine.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8-like 2 (EC:3.2.2.-, EC:4.2.99.18)
Alternative name(s):
DNA glycosylase/AP lyase Neil2
DNA-(apurinic or apyrimidinic site) lyase Neil2
Endonuclease VIII-like 2
Nei homolog 2
Short name:
NEH2
Nei-like protein 2
Gene namesi
Name:Neil2
Synonyms:Gm1212
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2686058. Neil2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • microtubule cytoskeleton Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001709092 – 329Endonuclease 8-like 2Add BLAST328

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50N6-acetyllysineBy similarity1
Modified residuei68PhosphoserineBy similarity1
Modified residuei150N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6R2P8.
PaxDbiQ6R2P8.
PRIDEiQ6R2P8.

PTM databases

PhosphoSitePlusiQ6R2P8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035121.
CleanExiMM_NEIL2.

Interactioni

Subunit structurei

Binds EP300.By similarity

GO - Molecular functioni

  • microtubule binding Source: MGI

Protein-protein interaction databases

BioGridi238543. 2 interactors.
STRINGi10090.ENSMUSP00000045200.

Structurei

3D structure databases

ProteinModelPortaliQ6R2P8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The zinc-finger domain is important for DNA binding.

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri280 – 316FPG-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IJ89. Eukaryota.
ENOG4112BNS. LUCA.
GeneTreeiENSGT00730000110955.
HOGENOMiHOG000069913.
HOVERGENiHBG082014.
InParanoidiQ6R2P8.
KOiK10568.
OMAiSPFVGQQ.
OrthoDBiEOG091G0G2K.
TreeFamiTF331502.

Family and domain databases

InterProiView protein in InterPro
IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. FPG_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
PfamiView protein in Pfam
PF06831. H2TH. 1 hit.
SMARTiView protein in SMART
SM01232. H2TH. 1 hit.
SUPFAMiSSF46946. SSF46946. 1 hit.
PROSITEiView protein in PROSITE
PS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6R2P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEGPSVRKF HHLVSPFVGQ KVVKTGGSSK KLHPAAFQSL WLQDAQVHGK
60 70 80 90 100
KLFLRFDPDE EMEPLNSSPQ PIQGMWQKEA VDRELALGPS AQEPSAGPSG
110 120 130 140 150
SGEPVPSRSA ETYNLGKIPS ADAQRWLEVR FGLFGSIWVN DFSRAKKANK
160 170 180 190 200
KGDWRDPVPR LVLHFSGGGF LVFYNCQMSW SPPPVIEPTC DILSEKFHRG
210 220 230 240 250
QALEALSQAQ PVCYTLLDQR YFSGLGNIIK NEALYRARIH PLSLGSCLSS
260 270 280 290 300
SSREALVDHV VEFSKDWLRD KFQGKERHTQ IYQKEQCPSG HQVMKETFGP
310 320
PDGLQRLTWW CPQCQPQLSS KGPQNLPSS
Length:329
Mass (Da):36,834
Last modified:September 18, 2013 - v4
Checksum:i221781934B9EB5A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti220R → K in AAR98807 (Ref. 1) Curated1
Sequence conflicti256L → F in AAR98807 (Ref. 1) Curated1
Sequence conflicti318L → P in AAR98807 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY518221 mRNA. Translation: AAR98807.1.
AC090654 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36068.1.
CCDSiCCDS36949.1.
RefSeqiNP_963904.2. NM_201610.2.
XP_006519262.1. XM_006519199.3.
UniGeneiMm.239490.

Genome annotation databases

EnsembliENSMUST00000038229; ENSMUSP00000045200; ENSMUSG00000035121.
GeneIDi382913.
KEGGimmu:382913.
UCSCiuc007uhk.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNEIL2_MOUSE
AccessioniPrimary (citable) accession number: Q6R2P8
Secondary accession number(s): G3X969
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: September 18, 2013
Last modified: July 5, 2017
This is version 100 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families