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Protein

Endonuclease 8-like 2

Gene

Neil2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).By similarity

Catalytic activityi

Removes damaged bases from DNA, leaving an abasic site.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Acetylation of Lys-50 leads to loss of DNA nicking activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNAPROSITE-ProRule annotation
Active sitei3 – 31Proton donorPROSITE-ProRule annotation
Active sitei50 – 501Proton donor; for beta-elimination activityPROSITE-ProRule annotation
Binding sitei227 – 2271DNABy similarity
Active sitei306 – 3061Proton donor; for delta-elimination activityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri280 – 31637FPG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_342323. Cleavage of the damaged pyrimidine.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8-like 2 (EC:3.2.2.-, EC:4.2.99.18)
Alternative name(s):
DNA glycosylase/AP lyase Neil2
DNA-(apurinic or apyrimidinic site) lyase Neil2
Endonuclease VIII-like 2
Nei homolog 2
Short name:
NEH2
Nei-like protein 2
Gene namesi
Name:Neil2
Synonyms:Gm1212
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2686058. Neil2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • microtubule cytoskeleton Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 329328Endonuclease 8-like 2PRO_0000170909Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501N6-acetyllysineBy similarity
Modified residuei150 – 1501N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ6R2P8.

PTM databases

PhosphoSiteiQ6R2P8.

Expressioni

Gene expression databases

CleanExiMM_NEIL2.
GenevestigatoriQ6R2P8.

Interactioni

Subunit structurei

Binds EP300.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6R2P8.
SMRiQ6R2P8. Positions 125-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The zinc-finger domain is important for DNA binding.

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation
Contains 1 FPG-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri280 – 31637FPG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG73546.
GeneTreeiENSGT00730000110955.
HOGENOMiHOG000069913.
HOVERGENiHBG082014.
InParanoidiQ6R2P8.
KOiK10568.
OMAiSVRKFHH.
OrthoDBiEOG7Z69CR.
TreeFamiTF331502.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6R2P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEGPSVRKF HHLVSPFVGQ KVVKTGGSSK KLHPAAFQSL WLQDAQVHGK
60 70 80 90 100
KLFLRFDPDE EMEPLNSSPQ PIQGMWQKEA VDRELALGPS AQEPSAGPSG
110 120 130 140 150
SGEPVPSRSA ETYNLGKIPS ADAQRWLEVR FGLFGSIWVN DFSRAKKANK
160 170 180 190 200
KGDWRDPVPR LVLHFSGGGF LVFYNCQMSW SPPPVIEPTC DILSEKFHRG
210 220 230 240 250
QALEALSQAQ PVCYTLLDQR YFSGLGNIIK NEALYRARIH PLSLGSCLSS
260 270 280 290 300
SSREALVDHV VEFSKDWLRD KFQGKERHTQ IYQKEQCPSG HQVMKETFGP
310 320
PDGLQRLTWW CPQCQPQLSS KGPQNLPSS
Length:329
Mass (Da):36,834
Last modified:September 18, 2013 - v4
Checksum:i221781934B9EB5A5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2201R → K in AAR98807 (Ref. 1) Curated
Sequence conflicti256 – 2561L → F in AAR98807 (Ref. 1) Curated
Sequence conflicti318 – 3181L → P in AAR98807 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY518221 mRNA. Translation: AAR98807.1.
AC090654 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36068.1.
CCDSiCCDS36949.1.
RefSeqiNP_963904.2. NM_201610.2.
XP_006519262.1. XM_006519199.2.
UniGeneiMm.239490.

Genome annotation databases

EnsembliENSMUST00000038229; ENSMUSP00000045200; ENSMUSG00000035121.
GeneIDi382913.
KEGGimmu:382913.
UCSCiuc007uhk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY518221 mRNA. Translation: AAR98807.1.
AC090654 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36068.1.
CCDSiCCDS36949.1.
RefSeqiNP_963904.2. NM_201610.2.
XP_006519262.1. XM_006519199.2.
UniGeneiMm.239490.

3D structure databases

ProteinModelPortaliQ6R2P8.
SMRiQ6R2P8. Positions 125-315.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ6R2P8.

Proteomic databases

PRIDEiQ6R2P8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038229; ENSMUSP00000045200; ENSMUSG00000035121.
GeneIDi382913.
KEGGimmu:382913.
UCSCiuc007uhk.1. mouse.

Organism-specific databases

CTDi252969.
MGIiMGI:2686058. Neil2.

Phylogenomic databases

eggNOGiNOG73546.
GeneTreeiENSGT00730000110955.
HOGENOMiHOG000069913.
HOVERGENiHBG082014.
InParanoidiQ6R2P8.
KOiK10568.
OMAiSVRKFHH.
OrthoDBiEOG7Z69CR.
TreeFamiTF331502.

Enzyme and pathway databases

ReactomeiREACT_342323. Cleavage of the damaged pyrimidine.

Miscellaneous databases

NextBioi403564.
PROiQ6R2P8.
SOURCEiSearch...

Gene expression databases

CleanExiMM_NEIL2.
GenevestigatoriQ6R2P8.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene identification signature analysis: a novel method for genome annotation."
    Ang C.C., Ng P.W.P., Wei C.L., Ruan Y.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Ola.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiNEIL2_MOUSE
AccessioniPrimary (citable) accession number: Q6R2P8
Secondary accession number(s): G3X969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: September 18, 2013
Last modified: May 27, 2015
This is version 83 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.