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Q6R2P8

- NEIL2_MOUSE

UniProt

Q6R2P8 - NEIL2_MOUSE

Protein

Endonuclease 8-like 2

Gene

Neil2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 4 (18 Sep 2013)
      Previous versions | rss
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    Functioni

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.By similarity

    Catalytic activityi

    Removes damaged bases from DNA, leaving an abasic site.
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

    Enzyme regulationi

    Acetylation of Lys-50 leads to loss of DNA nicking activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNAPROSITE-ProRule annotation
    Active sitei3 – 31Proton donorPROSITE-ProRule annotation
    Active sitei50 – 501Proton donor; for beta-elimination activityPROSITE-ProRule annotation
    Binding sitei227 – 2271DNABy similarity
    Active sitei306 – 3061Proton donor; for delta-elimination activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri280 – 31637FPG-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
    3. hydrolase activity, hydrolyzing N-glycosyl compounds Source: InterPro
    4. microtubule binding Source: MGI
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease 8-like 2 (EC:3.2.2.-, EC:4.2.99.18)
    Alternative name(s):
    DNA glycosylase/AP lyase Neil2
    DNA-(apurinic or apyrimidinic site) lyase Neil2
    Endonuclease VIII-like 2
    Nei homolog 2
    Short name:
    NEH2
    Nei-like protein 2
    Gene namesi
    Name:Neil2
    Synonyms:Gm1212
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:2686058. Neil2.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. microtubule cytoskeleton Source: MGI
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 329328Endonuclease 8-like 2PRO_0000170909Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501N6-acetyllysineBy similarity
    Modified residuei150 – 1501N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ6R2P8.

    PTM databases

    PhosphoSiteiQ6R2P8.

    Expressioni

    Gene expression databases

    CleanExiMM_NEIL2.
    GenevestigatoriQ6R2P8.

    Interactioni

    Subunit structurei

    Binds EP300.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6R2P8.
    SMRiQ6R2P8. Positions 115-315.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The zinc-finger domain is important for DNA binding.

    Sequence similaritiesi

    Belongs to the FPG family.PROSITE-ProRule annotation
    Contains 1 FPG-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri280 – 31637FPG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG73546.
    GeneTreeiENSGT00730000110955.
    HOGENOMiHOG000069913.
    HOVERGENiHBG082014.
    InParanoidiQ6R2P8.
    KOiK10568.
    OMAiSVRKFHH.
    OrthoDBiEOG7Z69CR.
    TreeFamiTF331502.

    Family and domain databases

    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6R2P8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEGPSVRKF HHLVSPFVGQ KVVKTGGSSK KLHPAAFQSL WLQDAQVHGK    50
    KLFLRFDPDE EMEPLNSSPQ PIQGMWQKEA VDRELALGPS AQEPSAGPSG 100
    SGEPVPSRSA ETYNLGKIPS ADAQRWLEVR FGLFGSIWVN DFSRAKKANK 150
    KGDWRDPVPR LVLHFSGGGF LVFYNCQMSW SPPPVIEPTC DILSEKFHRG 200
    QALEALSQAQ PVCYTLLDQR YFSGLGNIIK NEALYRARIH PLSLGSCLSS 250
    SSREALVDHV VEFSKDWLRD KFQGKERHTQ IYQKEQCPSG HQVMKETFGP 300
    PDGLQRLTWW CPQCQPQLSS KGPQNLPSS 329
    Length:329
    Mass (Da):36,834
    Last modified:September 18, 2013 - v4
    Checksum:i221781934B9EB5A5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti220 – 2201R → K in AAR98807. 1 PublicationCurated
    Sequence conflicti256 – 2561L → F in AAR98807. 1 PublicationCurated
    Sequence conflicti318 – 3181L → P in AAR98807. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY518221 mRNA. Translation: AAR98807.1.
    AC090654 Genomic DNA. No translation available.
    CH466535 Genomic DNA. Translation: EDL36068.1.
    CCDSiCCDS36949.1.
    RefSeqiNP_963904.2. NM_201610.2.
    XP_006519262.1. XM_006519199.1.
    UniGeneiMm.239490.

    Genome annotation databases

    EnsembliENSMUST00000038229; ENSMUSP00000045200; ENSMUSG00000035121.
    GeneIDi382913.
    KEGGimmu:382913.
    UCSCiuc007uhk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY518221 mRNA. Translation: AAR98807.1 .
    AC090654 Genomic DNA. No translation available.
    CH466535 Genomic DNA. Translation: EDL36068.1 .
    CCDSi CCDS36949.1.
    RefSeqi NP_963904.2. NM_201610.2.
    XP_006519262.1. XM_006519199.1.
    UniGenei Mm.239490.

    3D structure databases

    ProteinModelPortali Q6R2P8.
    SMRi Q6R2P8. Positions 115-315.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6R2P8.

    Proteomic databases

    PRIDEi Q6R2P8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000038229 ; ENSMUSP00000045200 ; ENSMUSG00000035121 .
    GeneIDi 382913.
    KEGGi mmu:382913.
    UCSCi uc007uhk.1. mouse.

    Organism-specific databases

    CTDi 252969.
    MGIi MGI:2686058. Neil2.

    Phylogenomic databases

    eggNOGi NOG73546.
    GeneTreei ENSGT00730000110955.
    HOGENOMi HOG000069913.
    HOVERGENi HBG082014.
    InParanoidi Q6R2P8.
    KOi K10568.
    OMAi SVRKFHH.
    OrthoDBi EOG7Z69CR.
    TreeFami TF331502.

    Miscellaneous databases

    NextBioi 403564.
    PROi Q6R2P8.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_NEIL2.
    Genevestigatori Q6R2P8.

    Family and domain databases

    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene identification signature analysis: a novel method for genome annotation."
      Ang C.C., Ng P.W.P., Wei C.L., Ruan Y.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129/Ola.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiNEIL2_MOUSE
    AccessioniPrimary (citable) accession number: Q6R2P8
    Secondary accession number(s): G3X969
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: September 18, 2013
    Last modified: October 1, 2014
    This is version 77 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3