Q6R0H6 (ALEX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ALEX

Alternative name(s):
Alternative gene product encoded by XL-exon

Gene names

Name:	Gnas
Synonyms:	Gnas1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May inhibit the adenylyl cyclase-stimulating activity of guanine nucleotide-binding protein G(s) subunit alpha which is produced from the same locus in a different open reading frame By similarity. UniProtKB P84996
Subunit structure	Interacts with the N-terminal region of the XLas isoforms of guanine nucleotide-binding protein G(s) subunit alpha By similarity. UniProtKB Q9Z213
Subcellular location	Cell membrane; Peripheral membrane protein. Cell projection › ruffle By similarity. Note: Predominantly associated with cell membrane ruffles By similarity. UniProtKB Q9Z213
Miscellaneous	This protein is produced by a bicistronic gene which also produces guanine nucleotide-binding protein G(s) subunit alpha from an overlapping reading frame By similarity. UniProtKB Q9Z213
Sequence similarities	Belongs to the ALEX family.
Sequence caution	The sequence BAC26987.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from mutant phenotype. Source: MGI activation of adenylate cyclase activity by G-protein signaling pathway Inferred from direct assay. Source: MGI behavior Inferred from mutant phenotype. Source: MGI cartilage development Inferred from mutant phenotype. Source: MGI embryonic cranial skeleton morphogenesis Inferred from mutant phenotype. Source: MGI embryonic hindlimb morphogenesis Inferred from mutant phenotype. Source: MGI endochondral ossification Inferred from mutant phenotype. Source: MGI energy reserve metabolic process Inferred from mutant phenotype. Source: MGI genetic imprinting Inferred from mutant phenotype. Source: MGI multicellular organism growth Inferred from mutant phenotype. Source: MGI positive regulation of osteoblast differentiation Inferred from mutant phenotype. Source: MGI positive regulation of osteoclast differentiation Inferred from mutant phenotype. Source: MGI post-embryonic body morphogenesis Inferred from mutant phenotype. Source: MGI response to drug Inferred from mutant phenotype. Source: MGI sensory perception of chemical stimulus Inferred from Biological aspect of Ancestor. Source: RefGenome skin development Inferred from mutant phenotype. Source: MGI tissue homeostasis Inferred from mutant phenotype. Source: MGI
Cellular component	heterotrimeric G-protein complex Traceable author statement. Source: MGI membrane fraction Inferred from direct assay. Source: MGI ruffle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein beta/gamma-subunit complex binding Inferred from Biological aspect of Ancestor. Source: RefGenome GTPase activity Traceable author statement. Source: MGI ionotropic glutamate receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome mu-type opioid receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome signal transducer activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Protein ALEX		PRO_0000253965
Regions
	Compositional bias	310 – 567	258	Pro-rich
Experimental info
	Sequence conflict	297	1	Q → E Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q6R0H6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 273F2244509B1573	FASTA	725	79,084
	10 20 30 40 50 60 MSPSPTRLAV RSVDPQKTPN LTSKAPARPS RKSEWVETTA HLRRKPCHSR HNSPAWEISG 70 80 90 100 110 120 PPWSSQDHLG PHQASKPSTQ RFWSPGPPLA RAQAWEPIPP HQKKLCHLSS TSLPRETIAS 130 140 150 160 170 180 LPCKSQTLRQ EVRKHWSPEL FPRSPGTSDL KTLASEKTTA LPLKNLCHFR SVEKNSGAIA 190 200 210 220 230 240 HPQDSRESSH KSALAASSRQ SRSRVRSASL PPRTRLPSGS EAPLTDHSAR LSDLLLTSHA 250 260 270 280 290 300 TAPRWRSPDP CLRLAEPPLG STTTPLSIWT APQSQVMARP SKSREPQIRA SAQRDPQLSE 310 320 330 340 350 360 KQPRWKEALP PPLRWKEKSP LRREGTDLPP SLKQWMPSQP LLPKPSLPDL MLELLRIPRC 370 380 390 400 410 420 SQIARAMPEK TGQPQERLQI SSRILKNSKK PQLSAPILTE GQPQSPQPLL PSPSLKAAEI 430 440 450 460 470 480 QPPSQPPRQS LPPRPSLPPG QPLSPRWSPQ PRQSLPPWRS LPPGQPLSPP RSPLPGQSPL 490 500 510 520 530 540 LEPIRPLEQS LAPQQCQPLL GQLPLGQPMQ VHWSGEPGHS QLLPPLGHPF LPAQQLPPGQ 550 560 570 580 590 600 PLLPAQSLLA GQPLPPPAGP ILDPPAPRSR LLTRLLRGLL RGRLPGLTST SGAEAAAGTR 610 620 630 640 650 660 HRLASARSSP PVMSRKKGPP AASSGFCGET AALACPGATR SGATQSATSS PEPSEAASVY 670 680 690 700 710 720 PSVPDHDPSA PGRPRILWRR GANRCAKKPL RCESRSAQIR NAASSSTSNW RRRRWTTCVH TACCF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"XL alpha-s, the extra-long form of the alpha subunit of the Gs G protein, is significantly longer than suspected, and so is its companion Alex." Abramowitz J., Grenet D., Birnbaumer M., Torres H.N., Birnbaumer L. Proc. Natl. Acad. Sci. U.S.A. 101:8366-8371(2004) [PubMed: 15148396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: FVB/N. Tissue: Brain.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 297-725. Strain: C57BL/6J. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY519502 mRNA. Translation: AAS00602.1. AL593857 Genomic DNA. Translation: CAO78033.1. AK030489 mRNA. Translation: BAC26987.1. Different initiation.
IPI	IPI00471141.
RefSeq	NP_963911.1. NM_201617.1. NP_963912.1. NM_201618.1.
UniGene	Mm.125770.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6R0H6.
2D gel databases
REPRODUCTION-2DPAGE	IPI00471141.
Proteomic databases
PRIDE	Q6R0H6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000109088; ENSMUSP00000104716; ENSMUSG00000027523.
GeneID	14683.
KEGG	mmu:14683.
UCSC	uc008oeu.1. mouse.
Organism-specific databases
CTD	2778.
MGI	MGI:95777. Gnas.
Phylogenomic databases
GeneTree	ENSGT00560000077005.
HOVERGEN	HBG080831.
Gene expression databases
ArrayExpress	Q6R0H6.
Bgee	Q6R0H6.
Genevestigator	Q6R0H6.
Family and domain databases
KO	K04632.
ProtoNet	Search...
Other
NextBio	286600.
SOURCE	Search...

Entry information

Entry name

ALEX_MOUSE

Accession

Primary (citable) accession number: Q6R0H6
Secondary accession number(s): A6PW72, Q8BIR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program