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Protein

NAD(P)H-hydrate epimerase

Gene

NAXE

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.UniRule annotation

Catalytic activityi

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi120 – 1201PotassiumUniRule annotation
Metal bindingi185 – 1851PotassiumUniRule annotation
Binding sitei218 – 2181NAD(P)HXUniRule annotation
Metal bindingi221 – 2211PotassiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-BTA-197264. Nicotinamide salvaging.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-hydrate epimeraseUniRule annotation (EC:5.1.99.6)
Alternative name(s):
Apolipoprotein A-I-binding proteinUniRule annotation
Short name:
AI-BPUniRule annotation
NAD(P)HX epimeraseBy similarity
Gene namesi
Name:NAXEBy similarity
Synonyms:AIBPUniRule annotation, APOA1BPUniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

  • Mitochondrion UniRule annotation
  • Secreted UniRule annotation

  • Note: In sperm, secretion gradually increases during capacitation.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232MitochondrionUniRule annotationAdd
BLAST
Chaini33 – 288256NAD(P)H-hydrate epimerasePRO_0000292422Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineBy similarity
Modified residuei144 – 1441N6-succinyllysineBy similarity

Post-translational modificationi

Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6QRN6.
PeptideAtlasiQ6QRN6.
PRIDEiQ6QRN6.

Interactioni

Subunit structurei

Homodimer. Interacts with APOA1 and APOA2.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000518.

Structurei

3D structure databases

ProteinModelPortaliQ6QRN6.
SMRiQ6QRN6. Positions 56-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 275211YjeF N-terminalUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 1235NAD(P)HXUniRule annotation
Regioni189 – 1957NAD(P)HXUniRule annotation

Sequence similaritiesi

Belongs to the NnrE/AIBP family.UniRule annotation
Contains 1 YjeF N-terminal domain.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiQ6QRN6.
KOiK17759.
OMAiHLGMFGY.
OrthoDBiEOG71K63V.
TreeFamiTF300197.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6QRN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLRALLGL GLLVAGSRLS RVRVQAGSCR AGATWWVPQR LISGGRGDSE
60 70 80 90 100
VMASSAVKYL SQEEAQAVDQ ELFNEYQFSV DQLMELAGLS CATAIAKAYP
110 120 130 140 150
PTSLSRSPPT VLVICGPGNN GGDGLVCARH LKLFGYQPTI YYPKRPNKPL
160 170 180 190 200
FTALVTQCQK MDIPFLGEMP PEPMLIDELY ELVVDAIFGF SFTGEVREPF
210 220 230 240 250
RSILSVLNGL TVPIASIDIP SGWDVEKGSS GGIQPDLLIS LTAPKKSATQ
260 270 280
FTGRYHYLGG RFVPPALEKK YQLNLPPYPD TECVYRLQ
Length:288
Mass (Da):31,363
Last modified:July 5, 2004 - v1
Checksum:iDEA84926DB23D8CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY528250 mRNA. Translation: AAS20598.1.
BC114830 mRNA. Translation: AAI14831.1.
RefSeqiNP_991365.1. NM_205796.3.
XP_005203675.1. XM_005203618.3.
UniGeneiBt.91150.

Genome annotation databases

EnsembliENSBTAT00000000518; ENSBTAP00000000518; ENSBTAG00000000404.
GeneIDi404132.
KEGGibta:404132.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY528250 mRNA. Translation: AAS20598.1.
BC114830 mRNA. Translation: AAI14831.1.
RefSeqiNP_991365.1. NM_205796.3.
XP_005203675.1. XM_005203618.3.
UniGeneiBt.91150.

3D structure databases

ProteinModelPortaliQ6QRN6.
SMRiQ6QRN6. Positions 56-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000518.

Proteomic databases

PaxDbiQ6QRN6.
PeptideAtlasiQ6QRN6.
PRIDEiQ6QRN6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000000518; ENSBTAP00000000518; ENSBTAG00000000404.
GeneIDi404132.
KEGGibta:404132.

Organism-specific databases

CTDi246703.

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiQ6QRN6.
KOiK17759.
OMAiHLGMFGY.
OrthoDBiEOG71K63V.
TreeFamiTF300197.

Enzyme and pathway databases

ReactomeiR-BTA-197264. Nicotinamide salvaging.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of differentially regulated genes in bovine blastocysts using an annealing control primer system."
    Hwang K.-C., Cui X.-S., Park S.-P., Shin M.-R., Park S.-Y., Kim E.-Y., Kim N.-H.
    Mol. Reprod. Dev. 69:43-51(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Blastocyst.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thymus.

Entry informationi

Entry nameiNNRE_BOVIN
AccessioniPrimary (citable) accession number: Q6QRN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.