Q6QNK2 (GP133_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable G-protein coupled receptor 133

Alternative name(s):
G-protein coupled receptor PGR25

Gene names

Name:	GPR133
Synonyms:	PGR25

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Orphan receptor.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 1 GPS domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Traceable author statement Ref.2. Source: GDB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.2. Source: GDB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6QNK2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6QNK2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-314: Missing.
     315-341: QTALNLTKTFLKAVGEILLLPGWIALS → MEKGTELLVSPSQSGPGGDQPLLVKHR

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6QNK2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-481: Missing.
     482-491: LITVHLKHRL → MHRVCFLSFQ

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 874	849	Probable G-protein coupled receptor 133		PRO_0000012905
Regions
	Topological domain	26 – 570	545	Extracellular Potential
	Transmembrane	571 – 591	21	Helical; Name=1; Potential
	Topological domain	592 – 602	11	Cytoplasmic Potential
	Transmembrane	603 – 623	21	Helical; Name=2; Potential
	Topological domain	624 – 633	10	Extracellular Potential
	Transmembrane	634 – 654	21	Helical; Name=3; Potential
	Topological domain	655 – 674	20	Cytoplasmic Potential
	Transmembrane	675 – 695	21	Helical; Name=4; Potential
	Topological domain	696 – 711	16	Extracellular Potential
	Transmembrane	712 – 732	21	Helical; Name=5; Potential
	Topological domain	733 – 760	28	Cytoplasmic Potential
	Transmembrane	761 – 781	21	Helical; Name=6; Potential
	Topological domain	782 – 784	3	Extracellular Potential
	Transmembrane	785 – 805	21	Helical; Name=7; Potential
	Topological domain	806 – 874	69	Cytoplasmic Potential
	Domain	507 – 556	50	GPS
Amino acid modifications
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Glycosylation	282	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	319	1	N-linked (GlcNAc...) Potential
	Glycosylation	394	1	N-linked (GlcNAc...) Potential
	Glycosylation	476	1	N-linked (GlcNAc...) Potential
	Glycosylation	501	1	N-linked (GlcNAc...) Potential
	Glycosylation	533	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 481	481	Missing in isoform 3.		VSP_012787
	Alternative sequence	1 – 314	314	Missing in isoform 2.		VSP_012788
	Alternative sequence	315 – 341	27	QTALN…WIALS → MEKGTELLVSPSQSGPGGDQ PLLVKHR in isoform 2.		VSP_012789
	Alternative sequence	482 – 491	10	LITVHLKHRL → MHRVCFLSFQ in isoform 3.		VSP_012790
	Natural variant	508	1	V → M. Corresponds to variant rs11833801 [ dbSNP | Ensembl ].		VAR_049461
	Natural variant	523	1	S → L. Corresponds to variant rs11061318 [ dbSNP | Ensembl ].		VAR_049462
Experimental info
	Sequence conflict	703	1	N → S in AAP35063. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8FC7C63D50F65E1B	FASTA	874	96,530
	10 20 30 40 50 60 MEKLLRLCCW YSWLLLFYYN FQVRGVYSRS QDHPGFQVLA SASHYWPLEN VDGIHELQDT 70 80 90 100 110 120 TGDIVEGKVN KGIYLKEEKG VTLLYYGRYN SSCISKPEQC GPEGVTFSFF WKTQGEQSRP 130 140 150 160 170 180 IPSAYGGQVI SNGFKVCSSG GRGSVELYTR DNSMTWEASF SPPGPYWTHV LFTWKSKEGL 190 200 210 220 230 240 KVYVNGTLST SDPSGKVSRD YGESNVNLVI GSEQDQAKCY ENGAFDEFII WERALTPDEI 250 260 270 280 290 300 AMYFTAAIGK HALLSSTLPS LFMTSTASPV MPTDAYHPII TNLTEERKTF QSPGVILSYL 310 320 330 340 350 360 QNVSLSLPSK SLSEQTALNL TKTFLKAVGE ILLLPGWIAL SEDSAVVLSL IDTIDTVMGH 370 380 390 400 410 420 VSSNLHGSTP QVTVEGSSAM AEFSVAKILP KTVNSSHYRF PAHGQSFIQI PHEAFHRHAW 430 440 450 460 470 480 STVVGLLYHS MHYYLNNIWP AHTKIAEAMH HQDCLLFATS HLISLEVSPP PTLSQNLSGS 490 500 510 520 530 540 PLITVHLKHR LTRKQHSEAT NSSNRVFVYC AFLDFSSGEG VWSNHGCALT RGNLTYSVCR 550 560 570 580 590 600 CTHLTNFAIL MQVVPLELAR GHQVALSSIS YVGCSLSVLC LVATLVTFAV LSSVSTIRNQ 610 620 630 640 650 660 RYHIHANLSF AVLVAQVLLL ISFRLEPGTT PCQVMAVLLH YFFLSAFAWM LVEGLHLYSM 670 680 690 700 710 720 VIKVFGSEDS KHRYYYGMGW GFPLLICIIS LSFAMDSYGT SNNCWLSLAS GAIWAFVAPA 730 740 750 760 770 780 LFVIVVNIGI LIAVTRVISQ ISADNYKIHG DPSAFKLTAK AVAVLLPILG TSWVFGVLAV 790 800 810 820 830 840 NGCAVVFQYM FATLNSLQGL FIFLFHCLLN SEVRAAFKHK TKVWSLTSSS ARTSNAKPFH 850 860 870 SDLMNGTRPG MASTKLSPWD KSSHSAHRVD LSAV
	Isoform 2 [UniParc]. Checksum: 3191A83261A5078D	FASTA	560	61,360
	10 20 30 40 50 60 MEKGTELLVS PSQSGPGGDQ PLLVKHREDS AVVLSLIDTI DTVMGHVSSN LHGSTPQVTV 70 80 90 100 110 120 EGSSAMAEFS VAKILPKTVN SSHYRFPAHG QSFIQIPHEA FHRHAWSTVV GLLYHSMHYY 130 140 150 160 170 180 LNNIWPAHTK IAEAMHHQDC LLFATSHLIS LEVSPPPTLS QNLSGSPLIT VHLKHRLTRK 190 200 210 220 230 240 QHSEATNSSN RVFVYCAFLD FSSGEGVWSN HGCALTRGNL TYSVCRCTHL TNFAILMQVV 250 260 270 280 290 300 PLELARGHQV ALSSISYVGC SLSVLCLVAT LVTFAVLSSV STIRNQRYHI HANLSFAVLV 310 320 330 340 350 360 AQVLLLISFR LEPGTTPCQV MAVLLHYFFL SAFAWMLVEG LHLYSMVIKV FGSEDSKHRY 370 380 390 400 410 420 YYGMGWGFPL LICIISLSFA MDSYGTSNNC WLSLASGAIW AFVAPALFVI VVNIGILIAV 430 440 450 460 470 480 TRVISQISAD NYKIHGDPSA FKLTAKAVAV LLPILGTSWV FGVLAVNGCA VVFQYMFATL 490 500 510 520 530 540 NSLQGLFIFL FHCLLNSEVR AAFKHKTKVW SLTSSSARTS NAKPFHSDLM NGTRPGMAST 550 560 KLSPWDKSSH SAHRVDLSAV
	Isoform 3 [UniParc]. Checksum: D108A3060EA93E60	FASTA	393	43,210
	10 20 30 40 50 60 MHRVCFLSFQ TRKQHSEATN SSNRVFVYCA FLDFSSGEGV WSNHGCALTR GNLTYSVCRC 70 80 90 100 110 120 THLTNFAILM QVVPLELARG HQVALSSISY VGCSLSVLCL VATLVTFAVL SSVSTIRNQR 130 140 150 160 170 180 YHIHANLSFA VLVAQVLLLI SFRLEPGTTP CQVMAVLLHY FFLSAFAWML VEGLHLYSMV 190 200 210 220 230 240 IKVFGSEDSK HRYYYGMGWG FPLLICIISL SFAMDSYGTS NNCWLSLASG AIWAFVAPAL 250 260 270 280 290 300 FVIVVNIGIL IAVTRVISQI SADNYKIHGD PSAFKLTAKA VAVLLPILGT SWVFGVLAVN 310 320 330 340 350 360 GCAVVFQYMF ATLNSLQGLF IFLFHCLLNS EVRAAFKHKT KVWSLTSSSA RTSNAKPFHS 370 380 390 DLMNGTRPGM ASTKLSPWDK SSHSAHRVDL SAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Bonner T.I., Nagle J.W., Kauffman D. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The human and mouse repertoire of the adhesion family of G-protein-coupled receptors." Bjarnadottir T.K., Fredriksson R., Hoeglund P.J., Gloriam D.E., Lagerstroem M.C., Schioeth H.B. Genomics 84:23-33(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The G protein-coupled receptor repertoires of human and mouse." Vassilatis D.K., Hohmann J.G., Zeng H., Li F., Ranchalis J.E., Mortrud M.T., Brown A., Rodriguez S.S., Weller J.R., Wright A.C., Bergmann J.E., Gaitanaris G.A. Proc. Natl. Acad. Sci. U.S.A. 100:4903-4908(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 355-708.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY532280 mRNA. Translation: AAS37682.1. AY278561 mRNA. Translation: AAP35063.1. AK131538 mRNA. Translation: BAD18674.1. BC112307 mRNA. Translation: AAI12308.1. BC112309 mRNA. Translation: AAI12310.1. AY255587 mRNA. Translation: AAO85099.1.
IPI	IPI00394960. IPI00552352. IPI00978724.
PIR	T47186.
RefSeq	NP_942122.2. NM_198827.3.
UniGene	Hs.719239.
3D structure databases
ProteinModelPortal	Q6QNK2.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000261654.
Protein family/group databases
MEROPS	S63.024.
GPCRDB	Search...
PTM databases
PhosphoSite	Q6QNK2.
Polymorphism databases
DMDM	59797935.
Proteomic databases
PaxDb	Q6QNK2.
PRIDE	Q6QNK2.
Protocols and materials databases
DNASU	283383.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261654; ENSP00000261654; ENSG00000111452. ENST00000376682; ENSP00000365872; ENSG00000111452. ENST00000543617; ENSP00000438021; ENSG00000111452.
GeneID	283383.
KEGG	hsa:283383.
UCSC	uc001uit.4. human. uc001uiv.1. human.
Organism-specific databases
CTD	283383.
GeneCards	GC12P131438.
HGNC	HGNC:19893. GPR133.
HPA	HPA042395.
MIM	613639. gene.
neXtProt	NX_Q6QNK2.
PharmGKB	PA134923933.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG247313.
HOVERGEN	HBG051779.
InParanoid	Q6QNK2.
KO	K08465.
OrthoDB	EOG4WSW91.
PhylomeDB	Q6QNK2.
Gene expression databases
ArrayExpress	Q6QNK2.
Bgee	Q6QNK2.
CleanEx	HS_GPR133.
Genevestigator	Q6QNK2.
GermOnline	ENSG00000111452. Homo sapiens.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR017981. GPCR_2-like. IPR000832. GPCR_2_secretin-like. IPR017983. GPCR_2_secretin-like_CS. IPR000203. GPS_dom. [Graphical view]
Pfam	PF00002. 7tm_2. 1 hit. PF01825. GPS. 1 hit. [Graphical view]
PRINTS	PR00249. GPCRSECRETIN.
SMART	SM00303. GPS. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00649. G_PROTEIN_RECEP_F2_1. False negative. PS00650. G_PROTEIN_RECEP_F2_2. 1 hit. PS50227. G_PROTEIN_RECEP_F2_3. False negative. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. PS50221. GPS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	283383.
NextBio	93855.
SOURCE	Search...

Entry information

Entry name

GP133_HUMAN

Accession

Primary (citable) accession number: Q6QNK2
Secondary accession number(s): Q2M1L3 , Q6ZMQ1, Q7Z7M2, Q86SM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.