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Protein

C-type lectin domain family 7 member A

Gene

Clec7a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.8 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi156Divalent metal cation1
Metal bindingi158Divalent metal cation1
Metal bindingi162Divalent metal cation1
Metal bindingi241Divalent metal cation1

GO - Molecular functioni

GO - Biological processi

  • cellular response to molecule of fungal origin Source: UniProtKB
  • inflammatory response Source: UniProtKB-KW
  • innate immune response Source: UniProtKB
  • leukocyte activation involved in immune response Source: UniProtKB
  • phagocytosis, recognition Source: Ensembl
  • stimulatory C-type lectin receptor signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Keywords - Ligandi

Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
C-type lectin domain family 7 member A
Alternative name(s):
Beta-glucan receptor
C-type lectin superfamily member 12
Dendritic cell-associated C-type lectin 1
Short name:
DC-associated C-type lectin 1
Short name:
Dectin-1
Gene namesi
Name:Clec7a
Synonyms:Bgr, Clecsf12, Dectin1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1861431. Clec7a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 49CytoplasmicSequence analysisAdd BLAST49
Transmembranei50 – 70Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini71 – 244ExtracellularSequence analysisAdd BLAST174

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi221W → A: Loss of glucan binding. Abolishes activation of NF-kappa-B. 1 Publication1
Mutagenesisi223H → A: Loss of glucan binding. Abolishes activation of NF-kappa-B. 1 Publication1
Mutagenesisi232C → A: Abolishes cell surface expression. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2034809.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002694931 – 244C-type lectin domain family 7 member AAdd BLAST244

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15Phosphotyrosine1 Publication1
Disulfide bondi119 ↔ 130PROSITE-ProRule annotation1 Publication
Disulfide bondi147 ↔ 240PROSITE-ProRule annotation1 Publication
Glycosylationi185N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi219 ↔ 232PROSITE-ProRule annotation1 Publication
Glycosylationi233N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication
Phosphorylated on tyrosine residues in response to glucan binding.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ6QLQ4.
PRIDEiQ6QLQ4.

PTM databases

iPTMnetiQ6QLQ4.
PhosphoSitePlusiQ6QLQ4.

Expressioni

Tissue specificityi

Detected in spleen (at protein level). Highly expressed in dendritic cells, spleen and thymus. Detected in epidermal Langerhans cells. Detected in macrophages, liver and lung.3 Publications

Gene expression databases

BgeeiENSMUSG00000079293.
CleanExiMM_CLEC7A.
ExpressionAtlasiQ6QLQ4. baseline and differential.
GenevisibleiQ6QLQ4. MM.

Interactioni

Subunit structurei

Homodimer (Probable). Interacts with SYK; participates in leukocyte activation in presence of fungal pathogens.Curated2 Publications

Structurei

Secondary structure

1244
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi124 – 126Combined sources3
Beta strandi129 – 138Combined sources10
Helixi140 – 149Combined sources10
Helixi160 – 170Combined sources11
Helixi171 – 173Combined sources3
Beta strandi176 – 183Combined sources8
Beta strandi185 – 189Combined sources5
Beta strandi201 – 203Combined sources3
Helixi212 – 214Combined sources3
Beta strandi219 – 223Combined sources5
Beta strandi226 – 230Combined sources5
Beta strandi236 – 242Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BPDX-ray1.50A/B113-244[»]
2BPEX-ray2.25A/B113-244[»]
2BPHX-ray2.20A/B113-244[»]
2CL8X-ray2.80A/B113-244[»]
ProteinModelPortaliQ6QLQ4.
SMRiQ6QLQ4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6QLQ4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 241C-type lectinPROSITE-ProRule annotationAdd BLAST116

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi15 – 18ITAM-like4

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00700000104266.
HOGENOMiHOG000220926.
HOVERGENiHBG105854.
InParanoidiQ6QLQ4.
TreeFamiTF336674.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR016187. CTDL_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6QLQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYHSHIENL DEDGYTQLDF STQDIHKRPR GSEKGSQAPS SPWRPIAVGL
60 70 80 90 100
GILCFVVVVV AAVLGALGEY GHNSGRNPEE KDNFLSRNKE NHKPTESSLD
110 120 130 140 150
EKVAPSKASQ TTGGFSQPCL PNWIMHGKSC YLFSFSGNSW YGSKRHCSQL
160 170 180 190 200
GAHLLKIDNS KEFEFIESQT SSHRINAFWI GLSRNQSEGP WFWEDGSAFF
210 220 230 240
PNSFQVRNTA PQESLLHNCV WIHGSEVYNQ ICNTSSYSIC EKEL
Length:244
Mass (Da):27,420
Last modified:October 3, 2012 - v3
Checksum:iC9435DD135D2F122
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37Q → R in AAF72710 (PubMed:10779524).Curated1
Sequence conflicti37Q → R in AAS37670 (PubMed:11544516).Curated1
Sequence conflicti37Q → R in AAH27742 (PubMed:15489334).Curated1
Sequence conflicti68 – 71GEYG → AFWR in AAF72710 (PubMed:10779524).Curated4
Sequence conflicti68 – 71GEYG → AFWR in AAS37670 (PubMed:11544516).Curated4
Sequence conflicti68 – 71GEYG → AFWR in AAH27742 (PubMed:15489334).Curated4
Sequence conflicti83N → S in AAS37670 (PubMed:11544516).Curated1
Sequence conflicti85L → P in AAH27742 (PubMed:15489334).Curated1
Sequence conflicti118P → S in AAF72710 (PubMed:10779524).Curated1
Sequence conflicti118P → S in AAS37670 (PubMed:11544516).Curated1
Sequence conflicti124I → T in AAH27742 (PubMed:15489334).Curated1
Sequence conflicti174 – 176RIN → HIT in AAH27742 (PubMed:15489334).Curated3
Sequence conflicti209 – 210TA → AV in AAS37670 (PubMed:11544516).Curated2
Sequence conflicti210A → V in AAF72710 (PubMed:10779524).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262985 mRNA. Translation: AAF72710.1.
AY534909 mRNA. Translation: AAS37670.1.
AC138620 Genomic DNA. No translation available.
BC027742 mRNA. Translation: AAH27742.1.
UniGeneiMm.239516.

Genome annotation databases

EnsembliENSMUST00000112076; ENSMUSP00000107707; ENSMUSG00000079293.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Mouse recombinant soluble Dectin-1 CTLD from insect cells origin

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262985 mRNA. Translation: AAF72710.1.
AY534909 mRNA. Translation: AAS37670.1.
AC138620 Genomic DNA. No translation available.
BC027742 mRNA. Translation: AAH27742.1.
UniGeneiMm.239516.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BPDX-ray1.50A/B113-244[»]
2BPEX-ray2.25A/B113-244[»]
2BPHX-ray2.20A/B113-244[»]
2CL8X-ray2.80A/B113-244[»]
ProteinModelPortaliQ6QLQ4.
SMRiQ6QLQ4.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

ChEMBLiCHEMBL2034809.

PTM databases

iPTMnetiQ6QLQ4.
PhosphoSitePlusiQ6QLQ4.

Proteomic databases

MaxQBiQ6QLQ4.
PRIDEiQ6QLQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112076; ENSMUSP00000107707; ENSMUSG00000079293.

Organism-specific databases

MGIiMGI:1861431. Clec7a.

Phylogenomic databases

GeneTreeiENSGT00700000104266.
HOGENOMiHOG000220926.
HOVERGENiHBG105854.
InParanoidiQ6QLQ4.
TreeFamiTF336674.

Enzyme and pathway databases

ReactomeiR-MMU-5607764. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

EvolutionaryTraceiQ6QLQ4.
PROiQ6QLQ4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000079293.
CleanExiMM_CLEC7A.
ExpressionAtlasiQ6QLQ4. baseline and differential.
GenevisibleiQ6QLQ4. MM.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR016187. CTDL_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLC7A_MOUSE
AccessioniPrimary (citable) accession number: Q6QLQ4
Secondary accession number(s): E9QPW2, Q8K1L4, Q9JI50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.