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Q6QLL4 (DHI1_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 1
EC=1.1.1.146
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase 1
Short name=11-DH
Short name=11-beta-HSD1
Gene names
Name:HSD11B1
OrganismCavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol By similarity. Ref.1

Catalytic activity

An 11-beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH.

Subunit structure

Homodimer. Ref.3

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein Ref.1.

Tissue specificity

Widely expressed at low levels. Highest expression in liver. Ref.1

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 300299Corticosteroid 11-beta-dehydrogenase isozyme 1
PRO_0000054618

Regions

Topological domain2 – 76Cytoplasmic Potential
Transmembrane8 – 2417Helical; Signal-anchor for type II membrane protein; Potential
Topological domain25 – 300276Lumenal Potential
Nucleotide binding41 – 6727NADP
Nucleotide binding92 – 932NADP
Nucleotide binding119 – 1235NADP
Nucleotide binding183 – 1875NADP
Nucleotide binding218 – 2225NADP

Sites

Active site1831Proton acceptor By similarity
Binding site1701Substrate By similarity

Amino acid modifications

Glycosylation2071N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict221S → P in AAF01249. Ref.1
Sequence conflict1031V → A in AAF01249. Ref.1

Secondary structure

1 ......................................... 300
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q6QLL4 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8D8A9725F7A6D912

FASTA30033,226
        10         20         30         40         50         60 
MAFLKKYLLT ILMVFLAYYY YSANEKFRPE MLQGKKVIVT GASKGIGREI AYHLAKMGAH 

        70         80         90        100        110        120 
VVVTARSKEA LQKVVARCLE LGAASAHYIA GSMEDMTFAE EFVAEAGNLM GGLDMLILNH 

       130        140        150        160        170        180 
VLYNRLTFFH GEIDNVRKSM EVNFHSFVVL SVAAMPMLMQ SQGSIAVVSS VAGKITYPLI 

       190        200        210        220        230        240 
APYSASKFAL DGFFSTLRSE FLVNKVNVSI TLCILGLIDT ETAIKATSGI YLGPASPKEE 

       250        260        270        280        290        300 
CALEIIKGTA LRQDEMYYVG SRWVPYLLGN PGRKIMEFLS AAEYNWDNVL SNEKLYGRWA

« Hide

References

[1]"Guinea pig 11beta-hydroxysteroid dehydrogenase type 1: primary structure and catalytic properties."
Pu X., Yang K.
Steroids 65:148-156(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: Hartley.
Tissue: Liver.
[2]"Guinea pig 11-beta-hydroxysteroid dehydrogenase type 1: species specific properties."
Odermatt A., Kadereit B.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The crystal structure of guinea pig 11beta-hydroxysteroid dehydrogenase type 1 provides a model for enzyme-lipid bilayer interactions."
Ogg D., Elleby B., Norstroem C., Stefansson K., Abrahmsen L., Oppermann U., Svensson S.
J. Biol. Chem. 280:3789-3794(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 25-297 IN COMPLEX WITH NADP, HOMODIMERIZATION.
[4]"N-(Pyridin-2-yl) arylsulfonamide inhibitors of 11beta-hydroxysteroid dehydrogenase type 1: Discovery of PF-915275."
Siu M., Johnson T.O., Wang Y., Nair S.K., Taylor W.D., Cripps S.J., Matthews J.J., Edwards M.P., Pauly T.A., Ermolieff J., Castro A., Hosea N.A., LaPaglia A., Fanjul A.N., Vogel J.E.
Bioorg. Med. Chem. Lett. 19:3493-3497(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 24-300 IN COMPLEX WITH NADP AND SYNTHETIC INHIBITOR.
[5]"Mutations of key hydrophobic surface residues of 11 beta-hydroxysteroid dehydrogenase type 1 increase solubility and monodispersity in a bacterial expression system."
Lawson A.J., Walker E.A., White S.A., Dafforn T.R., Stewart P.M., Ride J.P.
Protein Sci. 18:1552-1563(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 24-299 IN COMPLEX WITH NADP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF188005 mRNA. Translation: AAF01249.1.
AY535424 mRNA. Translation: AAS47491.1.
RefSeqNP_001166328.1. NM_001172857.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1XSEX-ray2.50A/B24-297[»]
3DWFX-ray2.20A/B/C/D24-299[»]
3G49X-ray2.50A/B/C/D24-300[»]
3LZ6X-ray1.84A/B/C/D26-288[»]
ProteinModelPortalQ6QLL4.
SMRQ6QLL4. Positions 24-297.
ModBaseSearch...

Protein-protein interaction databases

STRING10141.ENSCPOP00000005042.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSCPOT00000005656; ENSCPOP00000005042; ENSCPOG00000005596.
GeneID100135542.

Organism-specific databases

CTD3290.

Phylogenomic databases

eggNOGCOG1028.
GeneTreeENSGT00650000093378.
HOGENOMHOG000010276.
HOVERGENHBG005481.
InParanoidQ6QLL4.
OMASSIRTEY.
OrthoDBEOG405S1K.

Enzyme and pathway databases

BRENDA1.1.1.146. 1225.
SABIO-RKQ6QLL4.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ6QLL4.

Entry information

Entry nameDHI1_CAVPO
AccessionPrimary (citable) accession number: Q6QLL4
Secondary accession number(s): Q9QZE1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 71 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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