Reviewed, UniProtKB/Swiss-Prot Q6QA33 (DH12B_DANRE)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 12-B
    EC=1.1.1.62
Alternative name(s):
    17-beta-hydroxysteroid dehydrogenase 12-B
      Short name=17-beta-HSD 12-B
      Short name=zfHSD17B12B
      Short name=zf3.3

Gene names

Name:	hsd17b12b
ORF Names:	zgc:73289

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Developmental stage	Expressed throughout development. Weakly or not expressed in some adult organs. Ref.1
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Estradiol 17-beta-dehydrogenase 12-B		PRO_0000248372
Regions
	Transmembrane	8 – 28	21	Potential
	Transmembrane	125 – 145	21	Potential
	Transmembrane	269 – 289	21	Potential
	Nucleotide binding	48 – 77	30	NADP By similarity
Sites
	Active site	200	1	Proton acceptor By similarity
	Binding site	187	1	Substrate By similarity
Experimental info
	Sequence conflict	2	1	E → D in AAS58450. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6QA33-1 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: 995F46371555BFB4	FASTA	311	34,279
	10 20 30 40 50 60 MEPFADALFW VGAVTVLWLS VSSLWSLING IRVWILGNGN LMRASSLGKW AVVTGATDGI 70 80 90 100 110 120 GKAYAEELAR RGFAIVLISR TQEKLDEVSK AIESKYKVET KTISADFGSV DIYPKIESGL 130 140 150 160 170 180 AGLEIGVLVN NVGVSYSYPE FFLNIPDVDS FINNMININI MSVCQMTRLV LPRMVDRSKG 190 200 210 220 230 240 VILNVASASG MYPVPLLTLY SSTKAFVDFF SRGLDAEYKS KGIIIQSVLP FYVTTKLSKI 250 260 270 280 290 300 RKPTLDIPTP ERYVKAQLST IGLQTQSNGY LPHAIMGWVT ASLLPAKLLN KYVMGMGLSQ 310 RARYLKKQKQ G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of 17 beta-hydroxysteroid dehydrogenases in the zebrafish, Danio rerio." Mindnich R., Deluca D., Adamski J. Mol. Cell. Endocrinol. 215:19-30(2004) [PubMed: 15026171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY551080 mRNA. Translation: AAS58450.1. BC059617 mRNA. Translation: AAH59617.1.
IPI	IPI00488184.
RefSeq	NP_955907.1.
UniGene	Dr.20571
3D structure databases
SMR	Q6QA33. Positions 47-240, 48-280.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6QA33.
Proteomic databases
PRIDE	Q6QA33.
Genome annotation databases
Ensembl	ENSDART00000098842; ENSDARP00000089612; ENSDARG00000035872; Danio rerio. [Genome view]
GeneID	322626.
KEGG	dre:322626.
Organism-specific databases
CTD	322626.
ZFIN	ZDB-GENE-030131-1346. hsd17b12b.
Phylogenomic databases
eggNOG	fiNOG07308.
HOVERGEN	Q6QA33.
PhylomeDB	Q6QA33.
Enzyme and pathway databases
BRENDA	1.1.1.62. 96826.
Gene expression databases
ArrayExpress	Q6QA33.
Bgee	Q6QA33.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DH12B_DANRE

Accession

Primary (citable) accession number: Q6QA33
Secondary accession number(s): Q6PBR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	January 19, 2010
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project