Q6QA33 (DH12B_DANRE) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 59.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Estradiol 17-beta-dehydrogenase 12-B EC=1.1.1.62 Alternative name(s): 17-beta-hydroxysteroid dehydrogenase 12-B Short name=17-beta-HSD 12-B Short name=zf3.3 Short name=zfHSD17B12B | ||||
| Gene names |
| ||||
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) | ||||
| Taxonomic identifier | 7955 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio |
Protein attributes
| Sequence length | 311 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation By similarity. |
| Catalytic activity | Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. |
| Developmental stage | Expressed throughout development. Weakly or not expressed in some adult organs. Ref.1 |
| Sequence similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 311 | 311 | Estradiol 17-beta-dehydrogenase 12-B | PRO_0000248372 | |||||
Regions | |||||||||
| Transmembrane | 8 – 28 | 21 | Helical; Potential | ||||||
| Transmembrane | 125 – 145 | 21 | Helical; Potential | ||||||
| Transmembrane | 269 – 289 | 21 | Helical; Potential | ||||||
| Nucleotide binding | 48 – 77 | 30 | NADP By similarity | ||||||
Sites | |||||||||
| Active site | 200 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 187 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 2 | 1 | E → D in AAS58450. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification and characterization of 17 beta-hydroxysteroid dehydrogenases in the zebrafish, Danio rerio." Mindnich R., Deluca D., Adamski J. Mol. Cell. Endocrinol. 215:19-30(2004) [PubMed: 15026171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. |
| [2] | NIH - Zebrafish Gene Collection (ZGC) project Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY551080 mRNA. Translation: AAS58450.1. BC059617 mRNA. Translation: AAH59617.1. |
| IPI | IPI00488184. |
| RefSeq | NP_955907.1. NM_199613.1. |
| UniGene | Dr.20571. |
3D structure databases | |
| ProteinModelPortal | Q6QA33. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q6QA33. |
Proteomic databases | |
| PRIDE | Q6QA33. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSDART00000098842; ENSDARP00000089612; ENSDARG00000035872. |
| GeneID | 322626. |
| KEGG | dre:322626. |
Organism-specific databases | |
| CTD | 322626. |
| ZFIN | ZDB-GENE-030131-1346. hsd17b12b. |
Phylogenomic databases | |
| eggNOG | fiNOG07308. |
| GeneTree | ENSGT00390000010069. |
| HOVERGEN | HBG005478. |
| PhylomeDB | Q6QA33. |
Gene expression databases | |
| ArrayExpress | Q6QA33. |
| Bgee | Q6QA33. |
Family and domain databases | |
| InterPro | IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K00044. K10251. |
| Pfam | PF00106. adh_short. 1 hit. [Graphical view] |
| PRINTS | PR00081. GDHRDH. PR00080. SDRFAMILY. |
| PROSITE | PS00061. ADH_SHORT. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DH12B_DANRE | ||||||||
| Accession | Primary (citable) accession number: Q6QA33 Secondary accession number(s): Q6PBR0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with