Reviewed, UniProtKB/Swiss-Prot Q6Q8A5 (HXK2_TOBAC)

Names and origin

Protein names

Recommended name:
    Hexokinase-2, chloroplastic
    EC=2.7.1.1
Alternative name(s):
    NtHxK2

Gene names

Name:

HXK2

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. Ref.1
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Plastid › chloroplast stroma Probable.
Tissue specificity	Expressed in vascular starch sheath, xylem parenchyma, guard cells and root tips. Ref.1
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Chloroplast Potential
	Chain	31 – 499	469	Hexokinase-2, chloroplastic		PRO_0000247574

Sequences

	Sequence		Length	Mass (Da)
	Q6Q8A5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: FE40C67B74EA4812	FASTA	499	53,997
	10 20 30 40 50 60 MSVTVSSPAG RSFHISRSPY KKISKPRVII AAVRSGVSLA VAPILTKLQK DCATPLPVLR 70 80 90 100 110 120 HVADAMAVDM RAGLAVDGGS DLKMILSYID TLPTGNEKGL FYALDLGGTN FRVLRVQLGG 130 140 150 160 170 180 KEERVIATEF EQVSIPQELM FATSEELFDF IASELGKFSQ SEGGKFEMQQ GRTREIGFTF 190 200 210 220 230 240 SFPVKQTSVK SGILIKWTKG FAVSGTAGKD VVACLNEAME RQGLGMQVSA LVNDTVATLA 250 260 270 280 290 300 GARYWDNDVM VAVILGTGTN ACYVERVDAI PKLPQRMSNS PETIVNTEWG AFSNGLPLTE 310 320 330 340 350 360 FDREMDAESI NPGEQIFEKT ISGMYLGEIV RRVLVKMAKV GGLFGGGYVP EKLVTPFVLR 370 380 390 400 410 420 TPDICAMQQD TSRDLEAVES VLYDIAGVKS DLSARKTVVD ICDTIANRGG RLAGAGIVGI 430 440 450 460 470 480 LQKMEEDSKG VIFGKRTVVA MDGGLYEHYP QYREYLQEAV TELLGSEISK NVVIEHSKDG 490 SGIGAALLAA ANSKYEHDY

References

[1]

"Isolation and functional characterization of a novel plastidic hexokinase from Nicotiana tabacum." Giese J.-O., Herbers K., Hoffmann M., Kloesgen R.B., Sonnewald U. FEBS Lett. 579:827-831(2005) [PubMed: 15670855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY553215 mRNA. Translation: AAS60193.1.
3D structure databases
HSSP	HSSP built from PDB template 1BDG based on UniProtKB Q26609.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-12872.
BRENDA	2.7.1.1. 298.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProDom	PD001109. Hexokinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00378. HEXOKINASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK2_TOBAC

Accession

Primary (citable) accession number: Q6Q8A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)