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Protein

H/ACA ribonucleoprotein complex subunit 3

Gene

NOP10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs. Essential for growth.1 Publication

GO - Molecular functioni

  • RNA binding Source: SGD
  • snoRNA binding Source: InterPro

GO - Biological processi

  • cleavage involved in rRNA processing Source: SGD
  • rRNA pseudouridine synthesis Source: SGD
  • snRNA pseudouridine synthesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-31263-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit 3
Alternative name(s):
Nucleolar protein 10
Nucleolar protein family A member 3
snoRNP protein NOP10
Gene namesi
Name:NOP10
Ordered Locus Names:YHR072W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VIII

Organism-specific databases

CYGDiYHR072w-a.
EuPathDBiFungiDB:YHR072W-A.
SGDiS000007455. NOP10.

Subcellular locationi

GO - Cellular componenti

  • box H/ACA snoRNP complex Source: SGD
  • small nucleolar ribonucleoprotein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5858H/ACA ribonucleoprotein complex subunit 3PRO_0000149013Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6Q547.
PaxDbiQ6Q547.

Interactioni

Subunit structurei

Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs). The protein component of the H/ACA snoRNP contains CBF5, GAR1, NHP2 and NOP10. The complex contains a stable core composed of CBF5 and NOP10, to which GAR1 and NHP2 subsequently bind.1 Publication

Protein-protein interaction databases

BioGridi36506. 26 interactions.
DIPiDIP-5845N.
IntActiQ6Q547. 10 interactions.
MINTiMINT-2786302.
STRINGi4932.YHR072W-A.

Structurei

Secondary structure

1
58
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74Combined sources
Beta strandi8 – 125Combined sources
Beta strandi13 – 175Combined sources
Beta strandi28 – 314Combined sources
Beta strandi32 – 354Combined sources
Beta strandi37 – 393Combined sources
Helixi42 – 476Combined sources
Beta strandi49 – 513Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y2YNMR-A1-58[»]
2AQANMR-A2-58[»]
3U28X-ray1.90B1-58[»]
3UAIX-ray3.06B1-58[»]
DisProtiDP00475.
ProteinModelPortaliQ6Q547.
SMRiQ6Q547. Positions 1-48.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6Q547.

Family & Domainsi

Sequence similaritiesi

Belongs to the NOP10 family.Curated

Phylogenomic databases

eggNOGiCOG2260.
GeneTreeiENSGT00390000012563.
HOGENOMiHOG000159681.
InParanoidiQ6Q547.
KOiK11130.
OMAiKYSRERI.
OrthoDBiEOG7XSTTB.

Family and domain databases

InterProiIPR007264. H/ACA_rnp_Nop10.
[Graphical view]
PANTHERiPTHR13305. PTHR13305. 1 hit.
PfamiPF04135. Nop10p. 1 hit.
[Graphical view]
SUPFAMiSSF144210. SSF144210. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6Q547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLMYTLGPD GKRIYTLKKV TESGEITKSA HPARFSPDDK YSRQRVTLKK

RFGLVPGQ
Length:58
Mass (Da):6,636
Last modified:July 5, 2004 - v1
Checksum:i4718D35AF7911FBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. No translation available.
AY558548 Genomic DNA. Translation: AAS56874.1.
BK006934 Genomic DNA. Translation: DAA06766.1.
RefSeqiNP_058135.1. NM_001184438.1.

Genome annotation databases

EnsemblFungiiYHR072W-A; YHR072W-A; YHR072W-A.
GeneIDi856471.
KEGGisce:YHR072W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. No translation available.
AY558548 Genomic DNA. Translation: AAS56874.1.
BK006934 Genomic DNA. Translation: DAA06766.1.
RefSeqiNP_058135.1. NM_001184438.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y2YNMR-A1-58[»]
2AQANMR-A2-58[»]
3U28X-ray1.90B1-58[»]
3UAIX-ray3.06B1-58[»]
DisProtiDP00475.
ProteinModelPortaliQ6Q547.
SMRiQ6Q547. Positions 1-48.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36506. 26 interactions.
DIPiDIP-5845N.
IntActiQ6Q547. 10 interactions.
MINTiMINT-2786302.
STRINGi4932.YHR072W-A.

Proteomic databases

MaxQBiQ6Q547.
PaxDbiQ6Q547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR072W-A; YHR072W-A; YHR072W-A.
GeneIDi856471.
KEGGisce:YHR072W-A.

Organism-specific databases

CYGDiYHR072w-a.
EuPathDBiFungiDB:YHR072W-A.
SGDiS000007455. NOP10.

Phylogenomic databases

eggNOGiCOG2260.
GeneTreeiENSGT00390000012563.
HOGENOMiHOG000159681.
InParanoidiQ6Q547.
KOiK11130.
OMAiKYSRERI.
OrthoDBiEOG7XSTTB.

Enzyme and pathway databases

BioCyciYEAST:G3O-31263-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ6Q547.
NextBioi982137.
PROiQ6Q547.

Family and domain databases

InterProiIPR007264. H/ACA_rnp_Nop10.
[Graphical view]
PANTHERiPTHR13305. PTHR13305. 1 hit.
PfamiPF04135. Nop10p. 1 hit.
[Graphical view]
SUPFAMiSSF144210. SSF144210. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN H/ACA SNORNP COMPLEXES.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs."
    Henras A.K., Capeyrou R., Henry Y., Caizergues-Ferrer M.
    RNA 10:1704-1712(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE H/ACA SNORNP COMPLEX.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNOP10_YEAST
AccessioniPrimary (citable) accession number: Q6Q547
Secondary accession number(s): D3DL22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.