H/ACA ribonucleoprotein complex subunit 3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q6Q547 (NOP10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
H/ACA ribonucleoprotein complex subunit 3

Alternative name(s):
Nucleolar protein 10
Nucleolar protein family A member 3
snoRNP protein NOP10

Gene names

Name:	NOP10
Ordered Locus Names:	YHR072W-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	58 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs. Essential for growth. Ref.4
Subunit structure	Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs). The protein component of the H/ACA snoRNP contains CBF5, GAR1, NHP2 and NOP10. The complex contains a stable core composed of CBF5 and NOP10, to which GAR1 and NHP2 subsequently bind. Ref.4
Subcellular location	Nucleus › nucleolus Ref.4 Ref.5.
Sequence similarities	Belongs to the NOP10 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cleavage involved in rRNA processing Inferred from mutant phenotype Ref.4. Source: SGD rRNA pseudouridine synthesis Inferred from mutant phenotype Ref.4. Source: SGD snRNA pseudouridine synthesis Inferred from direct assay PubMed 15962000. Source: SGD
Cellular_component	box H/ACA snoRNP complex Inferred from physical interaction Ref.4. Source: SGD
Molecular_function	RNA binding Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
DBP2	P24783	1	EBI-2052475,EBI-5602
DBP3	P20447	1	EBI-2052475,EBI-5608
GAR1	P28007	1	EBI-2052475,EBI-7321
PRP43	P53131	1	EBI-2052475,EBI-505
PXR1	P53335	1	EBI-2052475,EBI-23652
RPA34	P47006	1	EBI-2052475,EBI-26109

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 58

H/ACA ribonucleoprotein complex subunit 3

PRO_0000149013

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q6Q547 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 4718D35AF7911FBA
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FASTA

6,636

        10         20         30         40         50 
MHLMYTLGPD GKRIYTLKKV TESGEITKSA HPARFSPDDK YSRQRVTLKK RFGLVPGQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs." Henras A., Henry Y., Bousquet-Antonelli C., Noaillac-Depeyre J., Gelugne J.-P., Caizergues-Ferrer M. EMBO J. 17:7078-7090(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN H/ACA SNORNP COMPLEXES.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs." Henras A.K., Capeyrou R., Henry Y., Caizergues-Ferrer M. RNA 10:1704-1712(2004) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF THE H/ACA SNORNP COMPLEX.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U10556 Genomic DNA. No translation available.
AY558548 Genomic DNA. Translation: AAS56874.1.
BK006934 Genomic DNA. Translation: DAA06766.1.

RefSeq

NP_058135.1. NM_001184438.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Y2Y	NMR	-	A	1-58	[»]
2AQA	NMR	-	A	2-58	[»]
3U28	X-ray	1.90	B	1-58	[»]
3UAI	X-ray	3.06	B	1-58	[»]

DisProt

DP00475.

ProteinModelPortal

Q6Q547.

SMR

Q6Q547. Positions 1-48.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5845N.

IntAct

Q6Q547. 8 interactions.

MINT

MINT-2786302.

STRING

4932.YHR072W-A.

Proteomic databases

PaxDb

Q6Q547.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YHR072W-A; YHR072W-A; YHR072W-A.

GeneID

856471.

KEGG

sce:YHR072W-A.

Organism-specific databases

CYGD

YHR072w-a.

SGD

S000007455. NOP10.

Phylogenomic databases

eggNOG

COG2260.

GeneTree

ENSGT00390000012563.

HOGENOM

HOG000159681.

K11130.

OMA

VYTLKKV.

OrthoDB

EOG4ZW8MX.

Gene expression databases

Genevestigator

Q6Q547.

GermOnline

YHR072W-A. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR007264. H/ACA_rnp_Nop10.
[Graphical view]

PANTHER

PTHR13305. PTHR13305. 1 hit.

Pfam

PF04135. Nop10p. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q6Q547.

NextBio

982137.

Entry information

Entry name

NOP10_YEAST

Accession

Primary (citable) accession number: Q6Q547
Secondary accession number(s): D3DL22

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents