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Q6Q547

- NOP10_YEAST

UniProt

Q6Q547 - NOP10_YEAST

Protein

H/ACA ribonucleoprotein complex subunit 3

Gene

NOP10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs. Essential for growth.1 Publication

    GO - Molecular functioni

    1. RNA binding Source: SGD
    2. snoRNA binding Source: InterPro

    GO - Biological processi

    1. cleavage involved in rRNA processing Source: SGD
    2. rRNA pseudouridine synthesis Source: SGD
    3. snRNA pseudouridine synthesis Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31263-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    H/ACA ribonucleoprotein complex subunit 3
    Alternative name(s):
    Nucleolar protein 10
    Nucleolar protein family A member 3
    snoRNP protein NOP10
    Gene namesi
    Name:NOP10
    Ordered Locus Names:YHR072W-A
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR072w-a.
    SGDiS000007455. NOP10.

    Subcellular locationi

    Nucleusnucleolus 2 Publications

    GO - Cellular componenti

    1. box H/ACA snoRNP complex Source: SGD
    2. small nucleolar ribonucleoprotein complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5858H/ACA ribonucleoprotein complex subunit 3PRO_0000149013Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6Q547.
    PaxDbiQ6Q547.

    Expressioni

    Gene expression databases

    GenevestigatoriQ6Q547.

    Interactioni

    Subunit structurei

    Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs). The protein component of the H/ACA snoRNP contains CBF5, GAR1, NHP2 and NOP10. The complex contains a stable core composed of CBF5 and NOP10, to which GAR1 and NHP2 subsequently bind.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DBP2P247831EBI-2052475,EBI-5602
    DBP3P204471EBI-2052475,EBI-5608
    GAR1P280071EBI-2052475,EBI-7321
    PRP43P531311EBI-2052475,EBI-505
    PXR1P533351EBI-2052475,EBI-23652
    RPA34P470061EBI-2052475,EBI-26109

    Protein-protein interaction databases

    BioGridi36506. 26 interactions.
    DIPiDIP-5845N.
    IntActiQ6Q547. 10 interactions.
    MINTiMINT-2786302.
    STRINGi4932.YHR072W-A.

    Structurei

    Secondary structure

    1
    58
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Beta strandi8 – 125
    Beta strandi13 – 175
    Beta strandi28 – 314
    Beta strandi32 – 354
    Beta strandi37 – 393
    Helixi42 – 476
    Beta strandi49 – 513

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Y2YNMR-A1-58[»]
    2AQANMR-A2-58[»]
    3U28X-ray1.90B1-58[»]
    3UAIX-ray3.06B1-58[»]
    DisProtiDP00475.
    ProteinModelPortaliQ6Q547.
    SMRiQ6Q547. Positions 1-48.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6Q547.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NOP10 family.Curated

    Phylogenomic databases

    eggNOGiCOG2260.
    GeneTreeiENSGT00390000012563.
    HOGENOMiHOG000159681.
    KOiK11130.
    OMAiERCKIRE.
    OrthoDBiEOG7XSTTB.

    Family and domain databases

    InterProiIPR007264. H/ACA_rnp_Nop10.
    [Graphical view]
    PANTHERiPTHR13305. PTHR13305. 1 hit.
    PfamiPF04135. Nop10p. 1 hit.
    [Graphical view]
    SUPFAMiSSF144210. SSF144210. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6Q547-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHLMYTLGPD GKRIYTLKKV TESGEITKSA HPARFSPDDK YSRQRVTLKK   50
    RFGLVPGQ 58
    Length:58
    Mass (Da):6,636
    Last modified:July 5, 2004 - v1
    Checksum:i4718D35AF7911FBA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10556 Genomic DNA. No translation available.
    AY558548 Genomic DNA. Translation: AAS56874.1.
    BK006934 Genomic DNA. Translation: DAA06766.1.
    RefSeqiNP_058135.1. NM_001184438.1.

    Genome annotation databases

    EnsemblFungiiYHR072W-A; YHR072W-A; YHR072W-A.
    GeneIDi856471.
    KEGGisce:YHR072W-A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10556 Genomic DNA. No translation available.
    AY558548 Genomic DNA. Translation: AAS56874.1 .
    BK006934 Genomic DNA. Translation: DAA06766.1 .
    RefSeqi NP_058135.1. NM_001184438.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Y2Y NMR - A 1-58 [» ]
    2AQA NMR - A 2-58 [» ]
    3U28 X-ray 1.90 B 1-58 [» ]
    3UAI X-ray 3.06 B 1-58 [» ]
    DisProti DP00475.
    ProteinModelPortali Q6Q547.
    SMRi Q6Q547. Positions 1-48.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36506. 26 interactions.
    DIPi DIP-5845N.
    IntActi Q6Q547. 10 interactions.
    MINTi MINT-2786302.
    STRINGi 4932.YHR072W-A.

    Proteomic databases

    MaxQBi Q6Q547.
    PaxDbi Q6Q547.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR072W-A ; YHR072W-A ; YHR072W-A .
    GeneIDi 856471.
    KEGGi sce:YHR072W-A.

    Organism-specific databases

    CYGDi YHR072w-a.
    SGDi S000007455. NOP10.

    Phylogenomic databases

    eggNOGi COG2260.
    GeneTreei ENSGT00390000012563.
    HOGENOMi HOG000159681.
    KOi K11130.
    OMAi ERCKIRE.
    OrthoDBi EOG7XSTTB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31263-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q6Q547.
    NextBioi 982137.
    PROi Q6Q547.

    Gene expression databases

    Genevestigatori Q6Q547.

    Family and domain databases

    InterProi IPR007264. H/ACA_rnp_Nop10.
    [Graphical view ]
    PANTHERi PTHR13305. PTHR13305. 1 hit.
    Pfami PF04135. Nop10p. 1 hit.
    [Graphical view ]
    SUPFAMi SSF144210. SSF144210. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN H/ACA SNORNP COMPLEXES.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. "Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs."
      Henras A.K., Capeyrou R., Henry Y., Caizergues-Ferrer M.
      RNA 10:1704-1712(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE H/ACA SNORNP COMPLEX.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNOP10_YEAST
    AccessioniPrimary (citable) accession number: Q6Q547
    Secondary accession number(s): D3DL22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3