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Protein

Aminopeptidase Q

Gene

LVRN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloprotease which may be important for placentation by regulating biological activity of key peptides at the embryo-maternal interface. On synthetic substrates it shows a marked preference for Leu-4-methylcoumaryl-7-amide (Leu-MCA) over Met-MCA, Arg-LCA and Lys-LCA. Cleaves the N-terminal amino acid of several peptides such as angiotensin-3, kisspeptin-10 and endokinin C.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by bestatin.1 Publication

Kineticsi

  1. KM=78.2 µM for Leu-4-methylcoumaryl-7-amide1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei240SubstrateBy similarity1
    Metal bindingi415Zinc; catalyticPROSITE-ProRule annotation1
    Active sitei416Proton acceptorPROSITE-ProRule annotation1
    Metal bindingi419Zinc; catalyticPROSITE-ProRule annotation1
    Metal bindingi438Zinc; catalyticPROSITE-ProRule annotation1
    Active sitei503Proton donorPROSITE-ProRule annotation1
    Sitei503Transition state stabilizerBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKQ6Q4G3.

    Protein family/group databases

    MEROPSiM01.026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase QCurated (EC:3.4.11.-1 Publication)
    Short name:
    AP-QCurated
    Short name:
    APQ1 Publication
    Alternative name(s):
    CHL2 antigen1 Publication
    Laeverin1 Publication
    Gene namesi
    Name:LVRNImported
    Synonyms:AQPEPImported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:26904. LVRN.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini2 – 13Cytoplasmic1 PublicationAdd BLAST12
    Transmembranei14 – 34Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini35 – 990Lumenal1 PublicationAdd BLAST956

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi206338.
    OpenTargetsiENSG00000172901.

    Polymorphism and mutation databases

    BioMutaiAQPEP.
    DMDMi296439457.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00002470682 – 990Aminopeptidase QAdd BLAST989

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi132N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi168N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi261N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi288N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi319N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi346N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi607N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi653N-linked (GlcNAc...)Sequence analysis1

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    EPDiQ6Q4G3.
    PaxDbiQ6Q4G3.
    PeptideAtlasiQ6Q4G3.
    PRIDEiQ6Q4G3.

    PTM databases

    iPTMnetiQ6Q4G3.
    PhosphoSitePlusiQ6Q4G3.

    Expressioni

    Tissue specificityi

    Specifically expressed in placenta and not in other tissues. Mainly found at the cell surface region of the extravillous trophoblasts. Detected on extravillous trophoblasts in the outer layer of the chorion laeve in the fetal membrane Not detected on either fetal amnionic epithelial cells or maternal decidual cells. Also detected in the migrating extravillous trophoblasts in the maternal decidual tissues (at protein level).1 Publication

    Gene expression databases

    BgeeiENSG00000172901.
    ExpressionAtlasiQ6Q4G3. baseline and differential.
    GenevisibleiQ6Q4G3. HS.

    Organism-specific databases

    HPAiHPA053254.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi128502. 1 interactor.
    STRINGi9606.ENSP00000350541.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6Q4G3.
    SMRiQ6Q4G3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni379 – 383Substrate bindingBy similarity5

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1046. Eukaryota.
    COG0308. LUCA.
    GeneTreeiENSGT00760000119082.
    InParanoidiQ6Q4G3.
    KOiK13724.
    OMAiPRNEIFF.
    OrthoDBiEOG091G01GH.
    PhylomeDBiQ6Q4G3.

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 2 hits.
    PfamiPF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q6Q4G3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MGPPSSSGFY VSRAVALLLA GLVAALLLAL AVLAALYGHC ERVPPSELPG
    60 70 80 90 100
    LRDLEAESSP PLRQKPTPTP KPSSARELAV TTTPSNWRPP GPWDQLRLPP
    110 120 130 140 150
    WLVPLHYDLE LWPQLRPDEL PAGSLPFTGR VNITVRCTVA TSRLLLHSLF
    160 170 180 190 200
    QDCERAEVRG PLSPGTGNAT VGRVPVDDVW FALDTEYMVL ELSEPLKPGS
    210 220 230 240 250
    SYELQLSFSG LVKEDLREGL FLNVYTDQGE RRALLASQLE PTFARYVFPC
    260 270 280 290 300
    FDEPALKATF NITMIHHPSY VALSNMPKLG QSEKEDVNGS KWTVTTFSTT
    310 320 330 340 350
    PHMPTYLVAF VICDYDHVNR TERGKEIRIW ARKDAIANGS ADFALNITGP
    360 370 380 390 400
    IFSFLEDLFN ISYSLPKTDI IALPSFDNHA MENWGLMIFD ESGLLLEPKD
    410 420 430 440 450
    QLTEKKTLIS YVVSHEIGHQ WFGNLVTMNW WNNIWLNEGF ASYFEFEVIN
    460 470 480 490 500
    YFNPKLPRNE IFFSNILHNI LREDHALVTR AVAMKVENFK TSEIQELFDI
    510 520 530 540 550
    FTYSKGASMA RMLSCFLNEH LFVSALKSYL KTFSYSNAEQ DDLWRHFQMA
    560 570 580 590 600
    IDDQSTVILP ATIKNIMDSW THQSGFPVIT LNVSTGVMKQ EPFYLENIKN
    610 620 630 640 650
    RTLLTSNDTW IVPILWIKNG TTQPLVWLDQ SSKVFPEMQV SDSDHDWVIL
    660 670 680 690 700
    NLNMTGYYRV NYDKLGWKKL NQQLEKDPKA IPVIHRLQLI DDAFSLSKNN
    710 720 730 740 750
    YIEIETALEL TKYLAEEDEI IVWHTVLVNL VTRDLVSEVN IYDIYSLLKR
    760 770 780 790 800
    YLLKRLNLIW NIYSTIIREN VLALQDDYLA LISLEKLFVT ACWLGLEDCL
    810 820 830 840 850
    QLSKELFAKW VDHPENEIPY PIKDVVLCYG IALGSDKEWD ILLNTYTNTT
    860 870 880 890 900
    NKEEKIQLAY AMSCSKDPWI LNRYMEYAIS TSPFTSNETN IIEVVASSEV
    910 920 930 940 950
    GRYVAKDFLV NNWQAVSKRY GTQSLINLIY TIGRTVTTDL QIVELQQFFS
    960 970 980 990
    NMLEEHQRIR VHANLQTIKN ENLKNKKLSA RIAAWLRRNT
    Length:990
    Mass (Da):113,283
    Last modified:May 18, 2010 - v4
    Checksum:i570A7072E66D0DCA
    GO
    Isoform 2 (identifier: Q6Q4G3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         698-701: KNNY → KGTY
         702-990: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:701
    Mass (Da):79,566
    Checksum:iD63CE25BF86F3C71
    GO
    Isoform 3 (identifier: Q6Q4G3-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-483: Missing.
         698-709: KNNYIEIETALE → NLQDFGHLKVPN
         710-990: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
    Show »
    Length:226
    Mass (Da):26,244
    Checksum:i500DAA17E0E09FD1
    GO
    Isoform 4 (identifier: Q6Q4G3-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-483: Missing.
         680-681: AI → MR
         682-990: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
    Show »
    Length:198
    Mass (Da):23,178
    Checksum:i97F6C600E390BD5B
    GO

    Sequence cautioni

    The sequence BAC11422 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti13R → H in AAS66719 (PubMed:14706636).Curated1
    Sequence conflicti54L → S in AAS66719 (PubMed:14706636).Curated1
    Sequence conflicti54L → S in BAF84344 (PubMed:14702039).Curated1
    Sequence conflicti54L → S in EAW48946 (Ref. 4) Curated1
    Sequence conflicti54L → S in AAH68560 (PubMed:15489334).Curated1
    Sequence conflicti54L → S in AAI09023 (PubMed:15489334).Curated1
    Sequence conflicti54L → S in AAI09024 (PubMed:15489334).Curated1
    Sequence conflicti374P → S in AAS66719 (PubMed:14706636).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_027059640V → F.Corresponds to variant rs17138632dbSNPEnsembl.1
    Natural variantiVAR_027060689L → F.3 PublicationsCorresponds to variant rs10078759dbSNPEnsembl.1
    Natural variantiVAR_027061936V → I.Corresponds to variant rs17138681dbSNPEnsembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0199151 – 483Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST483
    Alternative sequenceiVSP_019916680 – 681AI → MR in isoform 4. 1 Publication2
    Alternative sequenceiVSP_019917682 – 990Missing in isoform 4. 1 PublicationAdd BLAST309
    Alternative sequenceiVSP_019918698 – 709KNNYI…ETALE → NLQDFGHLKVPN in isoform 3. 1 PublicationAdd BLAST12
    Alternative sequenceiVSP_019919698 – 701KNNY → KGTY in isoform 2. 1 Publication4
    Alternative sequenceiVSP_019920702 – 990Missing in isoform 2. 1 PublicationAdd BLAST289
    Alternative sequenceiVSP_019921710 – 990Missing in isoform 3. 1 PublicationAdd BLAST281

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY560010 mRNA. Translation: AAS66719.1.
    AK075131 mRNA. Translation: BAC11422.1. Different initiation.
    AK291655 mRNA. Translation: BAF84344.1.
    AC010282 Genomic DNA. No translation available.
    AC034236 Genomic DNA. No translation available.
    CH471086 Genomic DNA. Translation: EAW48946.1.
    BC036440 mRNA. No translation available.
    BC045809 mRNA. No translation available.
    BC068560 mRNA. Translation: AAH68560.1.
    BC070028 mRNA. No translation available.
    BC094716 mRNA. No translation available.
    BC109022 mRNA. Translation: AAI09023.1.
    BC109023 mRNA. Translation: AAI09024.1.
    CCDSiCCDS4124.1. [Q6Q4G3-1]
    RefSeqiNP_776161.3. NM_173800.4. [Q6Q4G3-1]
    UniGeneiHs.556880.
    Hs.98288.

    Genome annotation databases

    EnsembliENST00000357872; ENSP00000350541; ENSG00000172901. [Q6Q4G3-1]
    ENST00000503329; ENSP00000427418; ENSG00000172901. [Q6Q4G3-4]
    ENST00000504467; ENSP00000423604; ENSG00000172901. [Q6Q4G3-2]
    ENST00000512314; ENSP00000427500; ENSG00000172901. [Q6Q4G3-4]
    ENST00000514509; ENSP00000427574; ENSG00000172901. [Q6Q4G3-3]
    GeneIDi206338.
    KEGGihsa:206338.
    UCSCiuc003kro.4. human. [Q6Q4G3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY560010 mRNA. Translation: AAS66719.1.
    AK075131 mRNA. Translation: BAC11422.1. Different initiation.
    AK291655 mRNA. Translation: BAF84344.1.
    AC010282 Genomic DNA. No translation available.
    AC034236 Genomic DNA. No translation available.
    CH471086 Genomic DNA. Translation: EAW48946.1.
    BC036440 mRNA. No translation available.
    BC045809 mRNA. No translation available.
    BC068560 mRNA. Translation: AAH68560.1.
    BC070028 mRNA. No translation available.
    BC094716 mRNA. No translation available.
    BC109022 mRNA. Translation: AAI09023.1.
    BC109023 mRNA. Translation: AAI09024.1.
    CCDSiCCDS4124.1. [Q6Q4G3-1]
    RefSeqiNP_776161.3. NM_173800.4. [Q6Q4G3-1]
    UniGeneiHs.556880.
    Hs.98288.

    3D structure databases

    ProteinModelPortaliQ6Q4G3.
    SMRiQ6Q4G3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi128502. 1 interactor.
    STRINGi9606.ENSP00000350541.

    Protein family/group databases

    MEROPSiM01.026.

    PTM databases

    iPTMnetiQ6Q4G3.
    PhosphoSitePlusiQ6Q4G3.

    Polymorphism and mutation databases

    BioMutaiAQPEP.
    DMDMi296439457.

    Proteomic databases

    EPDiQ6Q4G3.
    PaxDbiQ6Q4G3.
    PeptideAtlasiQ6Q4G3.
    PRIDEiQ6Q4G3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000357872; ENSP00000350541; ENSG00000172901. [Q6Q4G3-1]
    ENST00000503329; ENSP00000427418; ENSG00000172901. [Q6Q4G3-4]
    ENST00000504467; ENSP00000423604; ENSG00000172901. [Q6Q4G3-2]
    ENST00000512314; ENSP00000427500; ENSG00000172901. [Q6Q4G3-4]
    ENST00000514509; ENSP00000427574; ENSG00000172901. [Q6Q4G3-3]
    GeneIDi206338.
    KEGGihsa:206338.
    UCSCiuc003kro.4. human. [Q6Q4G3-1]

    Organism-specific databases

    CTDi206338.
    DisGeNETi206338.
    GeneCardsiLVRN.
    H-InvDBHIX0005107.
    HIX0164328.
    HGNCiHGNC:26904. LVRN.
    HPAiHPA053254.
    MIMi610046. gene.
    neXtProtiNX_Q6Q4G3.
    OpenTargetsiENSG00000172901.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG1046. Eukaryota.
    COG0308. LUCA.
    GeneTreeiENSGT00760000119082.
    InParanoidiQ6Q4G3.
    KOiK13724.
    OMAiPRNEIFF.
    OrthoDBiEOG091G01GH.
    PhylomeDBiQ6Q4G3.

    Enzyme and pathway databases

    SABIO-RKQ6Q4G3.

    Miscellaneous databases

    ChiTaRSiAQPEP. human.
    GenomeRNAii206338.
    PROiQ6Q4G3.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000172901.
    ExpressionAtlasiQ6Q4G3. baseline and differential.
    GenevisibleiQ6Q4G3. HS.

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 2 hits.
    PfamiPF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAMPQ_HUMAN
    AccessioniPrimary (citable) accession number: Q6Q4G3
    Secondary accession number(s): A8K6J0
    , C9JGD2, Q32MR1, Q4G0I9, Q4G0V2, Q86XA3, Q8NBZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: May 18, 2010
    Last modified: November 2, 2016
    This is version 105 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.