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Protein

Aminopeptidase Q

Gene

LVRN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Metalloprotease which may be important for placentation by regulating biological activity of key peptides at the embryo-maternal interface. On synthetic substrates it shows a marked preference for Leu-4-methylcoumaryl-7-amide (Leu-MCA) over Met-MCA, Arg-LCA and Lys-LCA. Cleaves the N-terminal amino acid of several peptides such as angiotensin-3, kisspeptin-10 and endokinin C.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by bestatin.1 Publication

Kineticsi

  1. KM=78.2 µM for Leu-4-methylcoumaryl-7-amide1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei240SubstrateBy similarity1
    Metal bindingi415Zinc; catalyticPROSITE-ProRule annotation1
    Active sitei416Proton acceptorPROSITE-ProRule annotation1
    Metal bindingi419Zinc; catalyticPROSITE-ProRule annotation1
    Metal bindingi438Zinc; catalyticPROSITE-ProRule annotation1
    Active sitei503Proton donorPROSITE-ProRule annotation1
    Sitei503Transition state stabilizerBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase, Metalloprotease, Protease
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKiQ6Q4G3

    Protein family/group databases

    MEROPSiM01.026

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase QCurated (EC:3.4.11.-1 Publication)
    Short name:
    AP-QCurated
    Short name:
    APQ1 Publication
    Alternative name(s):
    CHL2 antigen1 Publication
    Laeverin1 Publication
    Gene namesi
    Name:LVRNImported
    Synonyms:AQPEPImported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 5

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000172901.19
    HGNCiHGNC:26904 LVRN
    MIMi610046 gene
    neXtProtiNX_Q6Q4G3

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini2 – 13Cytoplasmic1 PublicationAdd BLAST12
    Transmembranei14 – 34Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini35 – 990Lumenal1 PublicationAdd BLAST956

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi206338
    OpenTargetsiENSG00000172901

    Chemistry databases

    ChEMBLiCHEMBL3831223

    Polymorphism and mutation databases

    BioMutaiAQPEP
    DMDMi296439457

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00002470682 – 990Aminopeptidase QAdd BLAST989

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi132N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi168N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi261N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi288N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi319N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi346N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi607N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi653N-linked (GlcNAc...) asparagineSequence analysis1

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ6Q4G3
    PeptideAtlasiQ6Q4G3
    PRIDEiQ6Q4G3
    ProteomicsDBi67267
    67268 [Q6Q4G3-2]
    67269 [Q6Q4G3-3]
    67270 [Q6Q4G3-4]

    PTM databases

    iPTMnetiQ6Q4G3
    PhosphoSitePlusiQ6Q4G3

    Expressioni

    Tissue specificityi

    Specifically expressed in placenta and not in other tissues. Mainly found at the cell surface region of the extravillous trophoblasts. Detected on extravillous trophoblasts in the outer layer of the chorion laeve in the fetal membrane Not detected on either fetal amnionic epithelial cells or maternal decidual cells. Also detected in the migrating extravillous trophoblasts in the maternal decidual tissues (at protein level).1 Publication

    Gene expression databases

    BgeeiENSG00000172901
    ExpressionAtlasiQ6Q4G3 baseline and differential
    GenevisibleiQ6Q4G3 HS

    Organism-specific databases

    HPAiHPA053254

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi128502, 2 interactors
    STRINGi9606.ENSP00000350541

    Structurei

    3D structure databases

    ProteinModelPortaliQ6Q4G3
    SMRiQ6Q4G3
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni379 – 383Substrate bindingBy similarity5

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1046 Eukaryota
    COG0308 LUCA
    GeneTreeiENSGT00760000119082
    InParanoidiQ6Q4G3
    KOiK13724
    OMAiNMTGYYR
    OrthoDBiEOG091G01GH
    PhylomeDBiQ6Q4G3

    Family and domain databases

    CDDicd09601 M1_APN_2, 1 hit
    InterProiView protein in InterPro
    IPR024571 ERAP1-like_C_dom
    IPR034016 M1_APN-typ
    IPR001930 Peptidase_M1
    IPR014782 Peptidase_M1_N
    PANTHERiPTHR11533 PTHR11533, 1 hit
    PfamiView protein in Pfam
    PF11838 ERAP1_C, 1 hit
    PF01433 Peptidase_M1, 1 hit
    PRINTSiPR00756 ALADIPTASE
    PROSITEiView protein in PROSITE
    PS00142 ZINC_PROTEASE, 1 hit

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q6Q4G3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MGPPSSSGFY VSRAVALLLA GLVAALLLAL AVLAALYGHC ERVPPSELPG
    60 70 80 90 100
    LRDLEAESSP PLRQKPTPTP KPSSARELAV TTTPSNWRPP GPWDQLRLPP
    110 120 130 140 150
    WLVPLHYDLE LWPQLRPDEL PAGSLPFTGR VNITVRCTVA TSRLLLHSLF
    160 170 180 190 200
    QDCERAEVRG PLSPGTGNAT VGRVPVDDVW FALDTEYMVL ELSEPLKPGS
    210 220 230 240 250
    SYELQLSFSG LVKEDLREGL FLNVYTDQGE RRALLASQLE PTFARYVFPC
    260 270 280 290 300
    FDEPALKATF NITMIHHPSY VALSNMPKLG QSEKEDVNGS KWTVTTFSTT
    310 320 330 340 350
    PHMPTYLVAF VICDYDHVNR TERGKEIRIW ARKDAIANGS ADFALNITGP
    360 370 380 390 400
    IFSFLEDLFN ISYSLPKTDI IALPSFDNHA MENWGLMIFD ESGLLLEPKD
    410 420 430 440 450
    QLTEKKTLIS YVVSHEIGHQ WFGNLVTMNW WNNIWLNEGF ASYFEFEVIN
    460 470 480 490 500
    YFNPKLPRNE IFFSNILHNI LREDHALVTR AVAMKVENFK TSEIQELFDI
    510 520 530 540 550
    FTYSKGASMA RMLSCFLNEH LFVSALKSYL KTFSYSNAEQ DDLWRHFQMA
    560 570 580 590 600
    IDDQSTVILP ATIKNIMDSW THQSGFPVIT LNVSTGVMKQ EPFYLENIKN
    610 620 630 640 650
    RTLLTSNDTW IVPILWIKNG TTQPLVWLDQ SSKVFPEMQV SDSDHDWVIL
    660 670 680 690 700
    NLNMTGYYRV NYDKLGWKKL NQQLEKDPKA IPVIHRLQLI DDAFSLSKNN
    710 720 730 740 750
    YIEIETALEL TKYLAEEDEI IVWHTVLVNL VTRDLVSEVN IYDIYSLLKR
    760 770 780 790 800
    YLLKRLNLIW NIYSTIIREN VLALQDDYLA LISLEKLFVT ACWLGLEDCL
    810 820 830 840 850
    QLSKELFAKW VDHPENEIPY PIKDVVLCYG IALGSDKEWD ILLNTYTNTT
    860 870 880 890 900
    NKEEKIQLAY AMSCSKDPWI LNRYMEYAIS TSPFTSNETN IIEVVASSEV
    910 920 930 940 950
    GRYVAKDFLV NNWQAVSKRY GTQSLINLIY TIGRTVTTDL QIVELQQFFS
    960 970 980 990
    NMLEEHQRIR VHANLQTIKN ENLKNKKLSA RIAAWLRRNT
    Length:990
    Mass (Da):113,283
    Last modified:May 18, 2010 - v4
    Checksum:i570A7072E66D0DCA
    GO
    Isoform 2 (identifier: Q6Q4G3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         698-701: KNNY → KGTY
         702-990: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:701
    Mass (Da):79,566
    Checksum:iD63CE25BF86F3C71
    GO
    Isoform 3 (identifier: Q6Q4G3-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-483: Missing.
         698-709: KNNYIEIETALE → NLQDFGHLKVPN
         710-990: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
    Show »
    Length:226
    Mass (Da):26,244
    Checksum:i500DAA17E0E09FD1
    GO
    Isoform 4 (identifier: Q6Q4G3-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-483: Missing.
         680-681: AI → MR
         682-990: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
    Show »
    Length:198
    Mass (Da):23,178
    Checksum:i97F6C600E390BD5B
    GO

    Sequence cautioni

    The sequence BAC11422 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti13R → H in AAS66719 (PubMed:14706636).Curated1
    Sequence conflicti54L → S in AAS66719 (PubMed:14706636).Curated1
    Sequence conflicti54L → S in BAF84344 (PubMed:14702039).Curated1
    Sequence conflicti54L → S in EAW48946 (Ref. 4) Curated1
    Sequence conflicti54L → S in AAH68560 (PubMed:15489334).Curated1
    Sequence conflicti54L → S in AAI09023 (PubMed:15489334).Curated1
    Sequence conflicti54L → S in AAI09024 (PubMed:15489334).Curated1
    Sequence conflicti374P → S in AAS66719 (PubMed:14706636).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_027059640V → F. Corresponds to variant dbSNP:rs17138632Ensembl.1
    Natural variantiVAR_027060689L → F3 PublicationsCorresponds to variant dbSNP:rs10078759Ensembl.1
    Natural variantiVAR_027061936V → I. Corresponds to variant dbSNP:rs17138681Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0199151 – 483Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST483
    Alternative sequenceiVSP_019916680 – 681AI → MR in isoform 4. 1 Publication2
    Alternative sequenceiVSP_019917682 – 990Missing in isoform 4. 1 PublicationAdd BLAST309
    Alternative sequenceiVSP_019918698 – 709KNNYI…ETALE → NLQDFGHLKVPN in isoform 3. 1 PublicationAdd BLAST12
    Alternative sequenceiVSP_019919698 – 701KNNY → KGTY in isoform 2. 1 Publication4
    Alternative sequenceiVSP_019920702 – 990Missing in isoform 2. 1 PublicationAdd BLAST289
    Alternative sequenceiVSP_019921710 – 990Missing in isoform 3. 1 PublicationAdd BLAST281

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY560010 mRNA Translation: AAS66719.1
    AK075131 mRNA Translation: BAC11422.1 Different initiation.
    AK291655 mRNA Translation: BAF84344.1
    AC010282 Genomic DNA No translation available.
    AC034236 Genomic DNA No translation available.
    CH471086 Genomic DNA Translation: EAW48946.1
    BC036440 mRNA No translation available.
    BC045809 mRNA No translation available.
    BC068560 mRNA Translation: AAH68560.1
    BC070028 mRNA No translation available.
    BC094716 mRNA No translation available.
    BC109022 mRNA Translation: AAI09023.1
    BC109023 mRNA Translation: AAI09024.1
    CCDSiCCDS4124.1 [Q6Q4G3-1]
    RefSeqiNP_776161.3, NM_173800.4 [Q6Q4G3-1]
    UniGeneiHs.556880
    Hs.98288

    Genome annotation databases

    EnsembliENST00000357872; ENSP00000350541; ENSG00000172901 [Q6Q4G3-1]
    ENST00000503329; ENSP00000427418; ENSG00000172901 [Q6Q4G3-4]
    ENST00000504467; ENSP00000423604; ENSG00000172901 [Q6Q4G3-2]
    ENST00000512314; ENSP00000427500; ENSG00000172901 [Q6Q4G3-4]
    ENST00000514509; ENSP00000427574; ENSG00000172901 [Q6Q4G3-3]
    GeneIDi206338
    KEGGihsa:206338
    UCSCiuc003kro.4 human [Q6Q4G3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiAMPQ_HUMAN
    AccessioniPrimary (citable) accession number: Q6Q4G3
    Secondary accession number(s): A8K6J0
    , C9JGD2, Q32MR1, Q4G0I9, Q4G0V2, Q86XA3, Q8NBZ2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: May 18, 2010
    Last modified: June 20, 2018
    This is version 117 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

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