Reviewed, UniProtKB/Swiss-Prot Q6Q4G3 (AMPQ_HUMAN)

Names and origin

Protein names

Recommended name:
    Aminopeptidase Q
      Short name=AP-Q
    EC=3.4.11.-
Alternative name(s):
    Laeverin
    CHL2 antigen

Gene names

Name:	AQPEP
Synonyms:	LVRN

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	990 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metalloprotease which may be important for placentation by regulating biological activity of key peptides at the embryo-maternal interface. On synthetic substrates it shows a marked preference for Leu-4-methylcoumaryl-7-amide (Leu-MCA) over Met-MCA, Arg-LCA and Lys-LCA. Cleaves the N-terminal amino acid of several peptides such as angiotensin-3, kisspeptin-10 and endokinin C. Ref.1 Ref.5
Cofactor	Binds 1 zinc ion per subunit By similarity.
Enzyme regulation	Inhibited by bestatin. Ref.5
Subunit structure	Homodimer. Ref.5
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Specifically expressed in placenta and not in other tissues. Mainly found at the cell surface region of the extravillous trophoblasts. Detected on extravillous trophoblasts in the outer layer of the chorion laeve in the fetal membrane Not detected on either fetal amnionic epithelial cells or maternal decidual cells. Also detected in the migrating extravillous trophoblasts in the maternal decidual tissues (at protein level). Ref.1
Post-translational modification	N-glycosylated. Ref.5
Sequence similarities	Belongs to the peptidase M1 family.
Biophysicochemical properties	Kinetic parameters: KM=78.2 µM for Leu-4-methylcoumaryl-7-amide pH dependence: Optimum pH is 7.0.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6Q4G3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6Q4G3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     698-701: KNNY → KGTY
     702-990: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6Q4G3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-483: Missing.
     698-709: KNNYIEIETALE → NLQDFGHLKVPN
     710-990: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 4 (identifier: Q6Q4G3-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-483: Missing.
     680-681: AI → MR
     682-990: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 990	989	Aminopeptidase Q		PRO_0000247068
Regions
	Topological domain	2 – 13	12	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Signal-anchor for type II membrane protein Potential
	Topological domain	35 – 990	956	Lumenal Potential
Sites
	Active site	416	1	By similarity
	Active site	503	1	Proton donor Potential
	Metal binding	415	1	Zinc; catalytic By similarity
	Metal binding	419	1	Zinc; catalytic By similarity
	Metal binding	438	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	132	1	N-linked (GlcNAc...) Potential
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	261	1	N-linked (GlcNAc...) Potential
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
	Glycosylation	319	1	N-linked (GlcNAc...) Potential
	Glycosylation	346	1	N-linked (GlcNAc...) Potential
	Glycosylation	607	1	N-linked (GlcNAc...) Potential
	Glycosylation	653	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 483	483	Missing in isoform 3 and isoform 4.		VSP_019915
	Alternative sequence	680 – 681	2	AI → MR in isoform 4.		VSP_019916
	Alternative sequence	682 – 990	309	Missing in isoform 4.		VSP_019917
	Alternative sequence	698 – 709	12	KNNYI…ETALE → NLQDFGHLKVPN in isoform 3.		VSP_019918
	Alternative sequence	698 – 701	4	KNNY → KGTY in isoform 2.		VSP_019919
	Alternative sequence	702 – 990	289	Missing in isoform 2.		VSP_019920
	Alternative sequence	710 – 990	281	Missing in isoform 3.		VSP_019921
	Natural variant	640	1	V → F: dbSNP rs17138632.		VAR_027059
	Natural variant	689	1	F → L: dbSNP rs10078759. Ref.4		VAR_027060
	Natural variant	936	1	V → I: dbSNP rs17138681.		VAR_027061
Experimental info
	Sequence conflict	13	1	R → H in AAS66719. Ref.1
	Sequence conflict	374	1	P → S in AAS66719. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C2F140FDA16FAE0B	FASTA	990	113,291
	10 20 30 40 50 60 MGPPSSSGFY VSRAVALLLA GLVAALLLAL AVLAALYGHC ERVPPSELPG LRDSEAESSP 70 80 90 100 110 120 PLRQKPTPTP KPSSARELAV TTTPSNWRPP GPWDQLRLPP WLVPLHYDLE LWPQLRPDEL 130 140 150 160 170 180 PAGSLPFTGR VNITVRCTVA TSRLLLHSLF QDCERAEVRG PLSPGTGNAT VGRVPVDDVW 190 200 210 220 230 240 FALDTEYMVL ELSEPLKPGS SYELQLSFSG LVKEDLREGL FLNVYTDQGE RRALLASQLE 250 260 270 280 290 300 PTFARYVFPC FDEPALKATF NITMIHHPSY VALSNMPKLG QSEKEDVNGS KWTVTTFSTT 310 320 330 340 350 360 PHMPTYLVAF VICDYDHVNR TERGKEIRIW ARKDAIANGS ADFALNITGP IFSFLEDLFN 370 380 390 400 410 420 ISYSLPKTDI IALPSFDNHA MENWGLMIFD ESGLLLEPKD QLTEKKTLIS YVVSHEIGHQ 430 440 450 460 470 480 WFGNLVTMNW WNNIWLNEGF ASYFEFEVIN YFNPKLPRNE IFFSNILHNI LREDHALVTR 490 500 510 520 530 540 AVAMKVENFK TSEIQELFDI FTYSKGASMA RMLSCFLNEH LFVSALKSYL KTFSYSNAEQ 550 560 570 580 590 600 DDLWRHFQMA IDDQSTVILP ATIKNIMDSW THQSGFPVIT LNVSTGVMKQ EPFYLENIKN 610 620 630 640 650 660 RTLLTSNDTW IVPILWIKNG TTQPLVWLDQ SSKVFPEMQV SDSDHDWVIL NLNMTGYYRV 670 680 690 700 710 720 NYDKLGWKKL NQQLEKDPKA IPVIHRLQFI DDAFSLSKNN YIEIETALEL TKYLAEEDEI 730 740 750 760 770 780 IVWHTVLVNL VTRDLVSEVN IYDIYSLLKR YLLKRLNLIW NIYSTIIREN VLALQDDYLA 790 800 810 820 830 840 LISLEKLFVT ACWLGLEDCL QLSKELFAKW VDHPENEIPY PIKDVVLCYG IALGSDKEWD 850 860 870 880 890 900 ILLNTYTNTT NKEEKIQLAY AMSCSKDPWI LNRYMEYAIS TSPFTSNETN IIEVVASSEV 910 920 930 940 950 960 GRYVAKDFLV NNWQAVSKRY GTQSLINLIY TIGRTVTTDL QIVELQQFFS NMLEEHQRIR 970 980 990 VHANLQTIKN ENLKNKKLSA RIAAWLRRNT
	Isoform 2. Checksum: E5518CC2F3C9633B	FASTA	701	79,574
	10 20 30 40 50 60 MGPPSSSGFY VSRAVALLLA GLVAALLLAL AVLAALYGHC ERVPPSELPG LRDSEAESSP 70 80 90 100 110 120 PLRQKPTPTP KPSSARELAV TTTPSNWRPP GPWDQLRLPP WLVPLHYDLE LWPQLRPDEL 130 140 150 160 170 180 PAGSLPFTGR VNITVRCTVA TSRLLLHSLF QDCERAEVRG PLSPGTGNAT VGRVPVDDVW 190 200 210 220 230 240 FALDTEYMVL ELSEPLKPGS SYELQLSFSG LVKEDLREGL FLNVYTDQGE RRALLASQLE 250 260 270 280 290 300 PTFARYVFPC FDEPALKATF NITMIHHPSY VALSNMPKLG QSEKEDVNGS KWTVTTFSTT 310 320 330 340 350 360 PHMPTYLVAF VICDYDHVNR TERGKEIRIW ARKDAIANGS ADFALNITGP IFSFLEDLFN 370 380 390 400 410 420 ISYSLPKTDI IALPSFDNHA MENWGLMIFD ESGLLLEPKD QLTEKKTLIS YVVSHEIGHQ 430 440 450 460 470 480 WFGNLVTMNW WNNIWLNEGF ASYFEFEVIN YFNPKLPRNE IFFSNILHNI LREDHALVTR 490 500 510 520 530 540 AVAMKVENFK TSEIQELFDI FTYSKGASMA RMLSCFLNEH LFVSALKSYL KTFSYSNAEQ 550 560 570 580 590 600 DDLWRHFQMA IDDQSTVILP ATIKNIMDSW THQSGFPVIT LNVSTGVMKQ EPFYLENIKN 610 620 630 640 650 660 RTLLTSNDTW IVPILWIKNG TTQPLVWLDQ SSKVFPEMQV SDSDHDWVIL NLNMTGYYRV 670 680 690 700 NYDKLGWKKL NQQLEKDPKA IPVIHRLQFI DDAFSLSKGT Y
	Isoform 3. Checksum: 500DAA17ED8DFFD1	FASTA	226	26,278
	10 20 30 40 50 60 MKVENFKTSE IQELFDIFTY SKGASMARML SCFLNEHLFV SALKSYLKTF SYSNAEQDDL 70 80 90 100 110 120 WRHFQMAIDD QSTVILPATI KNIMDSWTHQ SGFPVITLNV STGVMKQEPF YLENIKNRTL 130 140 150 160 170 180 LTSNDTWIVP ILWIKNGTTQ PLVWLDQSSK VFPEMQVSDS DHDWVILNLN MTGYYRVNYD 190 200 210 220 KLGWKKLNQQ LEKDPKAIPV IHRLQFIDDA FSLSNLQDFG HLKVPN
	Isoform 4. Checksum: 97F6C600E390BD5B	FASTA	198	23,178
	10 20 30 40 50 60 MKVENFKTSE IQELFDIFTY SKGASMARML SCFLNEHLFV SALKSYLKTF SYSNAEQDDL 70 80 90 100 110 120 WRHFQMAIDD QSTVILPATI KNIMDSWTHQ SGFPVITLNV STGVMKQEPF YLENIKNRTL 130 140 150 160 170 180 LTSNDTWIVP ILWIKNGTTQ PLVWLDQSSK VFPEMQVSDS DHDWVILNLN MTGYYRVNYD 190 KLGWKKLNQQ LEKDPKMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human extravillous trophoblasts express laeverin, a novel protein that belongs to membrane-bound gluzincin metallopeptidases." Fujiwara H., Higuchi T., Yamada S., Hirano T., Sato Y., Nishioka Y., Yoshioka S., Tatsumi K., Ueda M., Maeda M., Fujii S. Biochem. Biophys. Res. Commun. 313:962-968(2004) [PubMed: 14706636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 2-12; 269-277; 590-599; 811-820 AND 838-847, POSSIBLE FUNCTION, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 435-990. Tissue: Placenta.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4), VARIANT LEU-689. Tissue: Placenta and Testis.
[5]	"Laeverin/aminopeptidase Q, a novel bestatin-sensitive leucine aminopeptidase belonging to the M1 family of aminopeptidases." Maruyama M., Hattori A., Goto Y., Ueda M., Maeda M., Fujiwara H., Tsujimoto M. J. Biol. Chem. 282:20088-20096(2007) [PubMed: 17525158] [Abstract] Cited for: PROTEIN SEQUENCE OF 65-74, FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, GLYCOSYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY560010 mRNA. Translation: AAS66719.1. AK075131 mRNA. Translation: BAC11422.1. Different initiation. AK291655 mRNA. Translation: BAF84344.1. CH471086 Genomic DNA. Translation: EAW48946.1. BC036440 mRNA. No translation available. BC045809 mRNA. No translation available. BC068560 mRNA. Translation: AAH68560.1. BC070028 mRNA. No translation available. BC094716 mRNA. No translation available. BC109022 mRNA. Translation: AAI09023.1. BC109023 mRNA. Translation: AAI09024.1.
IPI	IPI00419976. IPI00719130. IPI00783775. IPI00884099.
RefSeq	NP_776161.3.
UniGene	Hs.98288
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6Q4G3.
Protein family/group databases
MEROPS	M01.026.
Proteomic databases
PRIDE	Q6Q4G3.
Genome annotation databases
Ensembl	ENST00000357872; ENSP00000350541; ENSG00000172901; Homo sapiens. [Genome view]
GeneID	206338.
KEGG	hsa:206338.
Organism-specific databases
CTD	206338.
GeneCards	GC05P115327.
MIM	610046. gene.
Phylogenomic databases
HOGENOM	Q6Q4G3.
HOVERGEN	Q6Q4G3.
Gene expression databases
ArrayExpress	Q6Q4G3.
Bgee	Q6Q4G3.
Genevestigator	Q6Q4G3.
GermOnline	ENSG00000172901. Homo sapiens.
Family and domain databases
InterPro	IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

AMPQ_HUMAN

Accession

Primary (citable) accession number: Q6Q4G3
Secondary accession number(s): A8K6J0 , Q32MR1, Q4G0I9, Q4G0V2, Q86XA3, Q8NBZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 46 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.