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Protein

Plasma membrane calcium-transporting ATPase 4

Gene

Atp2b4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell (By similarity). By regulating sperm cell calcium homeostasis, may play a role in sperm motility (PubMed:15078889).By similarity1 Publication

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).By similarity

Enzyme regulationi

Activated by calcium/calmodulin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4664-aspartylphosphate intermediateBy similarity1
Metal bindingi786MagnesiumBy similarity1
Metal bindingi790MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-418359. Reduction of cytosolic Ca++ levels.
R-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 4Curated (EC:3.6.3.8By similarity)
Short name:
PMCA41 Publication
Gene namesi
Name:Atp2b4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88111. Atp2b4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 100CytoplasmicCuratedAdd BLAST100
Transmembranei101 – 121HelicalSequence analysisAdd BLAST21
Topological domaini122 – 147ExtracellularCuratedAdd BLAST26
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 369CytoplasmicCuratedAdd BLAST201
Transmembranei370 – 390HelicalSequence analysisAdd BLAST21
Topological domaini391 – 409ExtracellularCuratedAdd BLAST19
Transmembranei410 – 430HelicalSequence analysisAdd BLAST21
Topological domaini431 – 844CytoplasmicCuratedAdd BLAST414
Transmembranei845 – 865HelicalSequence analysisAdd BLAST21
Topological domaini866 – 872ExtracellularCurated7
Transmembranei873 – 893HelicalSequence analysisAdd BLAST21
Topological domaini894 – 919CytoplasmicCuratedAdd BLAST26
Transmembranei920 – 942HelicalSequence analysisAdd BLAST23
Topological domaini943 – 956ExtracellularCuratedAdd BLAST14
Transmembranei957 – 979HelicalSequence analysisAdd BLAST23
Topological domaini980 – 995CytoplasmicCuratedAdd BLAST16
Transmembranei996 – 1016HelicalSequence analysisAdd BLAST21
Topological domaini1017 – 1029ExtracellularCuratedAdd BLAST13
Transmembranei1030 – 1050HelicalSequence analysisAdd BLAST21
Topological domaini1051 – 1205CytoplasmicCuratedAdd BLAST155

GO - Cellular componenti

  • basolateral plasma membrane Source: Ensembl
  • integral component of plasma membrane Source: MGI
  • intracellular membrane-bounded organelle Source: GO_Central
  • membrane Source: MGI
  • neuron projection Source: Ensembl
  • plasma membrane Source: MGI
  • sarcolemma Source: BHF-UCL
  • sperm flagellum Source: UniProtKB
  • sperm principal piece Source: Ensembl
  • T-tubule Source: BHF-UCL
  • Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

Male mice lacking Atp2b4 are infertile with severe reduction of sperm motility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004351241 – 1205Plasma membrane calcium-transporting ATPase 4Add BLAST1205

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei329PhosphoserineCombined sources1
Modified residuei335PhosphoserineBy similarity1
Modified residuei1065PhosphoserineBy similarity1
Modified residuei1071PhosphoserineBy similarity1
Modified residuei1072Omega-N-methylarginineCombined sources1
Modified residuei1103Phosphothreonine; by PKCBy similarity1
Modified residuei1145PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PRIDEiQ6Q477.

PTM databases

iPTMnetiQ6Q477.
PhosphoSitePlusiQ6Q477.

Expressioni

Tissue specificityi

Specifically expressed by sperm in testis (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000026463.
ExpressionAtlasiQ6Q477. baseline and differential.

Interactioni

Subunit structurei

Interacts with PDZD11. Interacts with SLC35G1 and STIM1. Interacts with calmodulin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-46143N.
IntActiQ6Q477. 1 interactor.
STRINGi10090.ENSMUSP00000119242.

Structurei

3D structure databases

ProteinModelPortaliQ6Q477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1087 – 1104Calmodulin-binding subdomain ABy similarityAdd BLAST18
Regioni1105 – 1114Calmodulin-binding subdomain BBy similarity10

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
KOiK05850.
OMAiIGDTHYH.
OrthoDBiEOG091G057D.
PhylomeDBiQ6Q477.
TreeFamiTF300330.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR030319. ATP2B1/4.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF276. PTHR24093:SF276. 2 hits.
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6Q477-1) [UniParc]FASTAAdd to basket
Also known as: PMCA4b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNPPGQSVS ANTVAESHEG EFGCTLMDLR KLMELRGADA VAQISAHYGG
60 70 80 90 100
VQEICTRLKT SPIEGLSGNP ADLEKRRLVF GKNVIPPKRP KTFLELVWEA
110 120 130 140 150
LQDVTLIILE IAAIISLVLS FYRPPGGDNE ICGHIASSPE EEEEGETGWI
160 170 180 190 200
EGAAILASVI IVVLVTAFND WSKEKQFRGL QSRIELEQKF SIIRNGQLIQ
210 220 230 240 250
LPVAEIVVGD IAQIKYGDLL PADGILIQGN DLKIDESSLT GESDHVKKTL
260 270 280 290 300
DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIIFTLLGAS EEEDDDDKKK
310 320 330 340 350
KGKKQGAPEN RNKAKTQDGV ALEIQPLNSQ EGLDSEDKEK KIARIPKKEK
360 370 380 390 400
SVLQGKLTRL AVQIGKAGLI MSVLTVVILI LYFVVDNFVI QRREWLPECT
410 420 430 440 450
PVYIQYFVKF FIIGVTVLVV AVPEGLPLAV TISLAYSVKK MMKDNNLVRH
460 470 480 490 500
LDACETMGNA TAICSDKTGT LTMNRMTVVQ AYIGGTHYRQ IPQPDVFPPK
510 520 530 540 550
VLELIVNGIS INCAYTSKIQ PPEKEGGLPR QVGNKTECGL LGFVTDLKQD
560 570 580 590 600
YQAVRNEVPE EKLFKVYTFN SVRKSMSTVI RKPEGGFRMF SKGASEIMLR
610 620 630 640 650
RCDRILNKEG EIKSFRSKDR DNMVRNVIEP MASEGLRTIC LAYRDFDGTE
660 670 680 690 700
PSWDIEGEIL TSLICIAVVG IEDPVRPEVP DAIAKCKRAG ITVRMVTGDN
710 720 730 740 750
VNTARAIATK CGILTPKDDF LCLEGKEFNS LIRNEKGEVE QEKLDKIWPK
760 770 780 790 800
LRVLARSSPT DKHTLVKGII DSTAGEQRQV VAVTGDGTND GPALKKADVG
810 820 830 840 850
FAMGIAGTDV AKEASDIILT DDNFTSIVKA VMWGRNVYDS ISKFLQFQLT
860 870 880 890 900
VNVVAVIVAF TGACITQDSP LKAVQMLWVN LIMDTFASLA LATEPPTESL
910 920 930 940 950
LRRRPYGRNK PLISRTMMKN ILGHAVYQLL IVFLLVFAGD TLFDIDSGRK
960 970 980 990 1000
APLNSPPSQH YTIVFNTFVL MQLFNEINAR KIHGEKNVFA GVYRNIIFCT
1010 1020 1030 1040 1050
VVLGTFFCQI MIVELGGKPF SCTSLTMEQW MWCLFIGIGE LLWGQVISAI
1060 1070 1080 1090 1100
PTKSLKFLKE AGHGSDKEDI SRDTEGMDEI DLAEMELRRG QILWVRGLNR
1110 1120 1130 1140 1150
IQTQIRVVKL FHNNHEVAHK PKNRSSIHTF MTQPEYPADD ELSQSFLDIQ
1160 1170 1180 1190 1200
EGNPELVSKA GTSVLLLDGE AASHDNINNN AVDCHQVQIV ASHSDSPLPS

LETPV
Length:1,205
Mass (Da):133,069
Last modified:July 5, 2004 - v1
Checksum:i75E39DB859951DF0
GO
Isoform 2 (identifier: Q6Q477-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1104-1107: QIRV → QEGN
     1108-1205: Missing.

Show »
Length:1,107
Mass (Da):122,295
Checksum:iB43E6ED7DDF7EC1D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0580181104 – 1107QIRV → QEGN in isoform 2. 4
Alternative sequenceiVSP_0580191108 – 1205Missing in isoform 2. Add BLAST98

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY560895 mRNA. Translation: AAT01506.1.
AC124338 Genomic DNA. No translation available.
AC157924 Genomic DNA. No translation available.
BC109172 mRNA. Translation: AAI09173.1.
BC109173 mRNA. Translation: AAI09174.1.
CCDSiCCDS15298.1. [Q6Q477-1]
RefSeqiNP_001161421.1. NM_001167949.2.
NP_998781.1. NM_213616.4. [Q6Q477-1]
XP_006529786.1. XM_006529723.2. [Q6Q477-1]
XP_006529787.1. XM_006529724.3. [Q6Q477-1]
XP_006529788.1. XM_006529725.2. [Q6Q477-1]
UniGeneiMm.188617.

Genome annotation databases

EnsembliENSMUST00000112264; ENSMUSP00000107883; ENSMUSG00000026463. [Q6Q477-2]
ENSMUST00000143567; ENSMUSP00000119242; ENSMUSG00000026463. [Q6Q477-1]
ENSMUST00000165602; ENSMUSP00000133187; ENSMUSG00000026463. [Q6Q477-1]
GeneIDi381290.
KEGGimmu:381290.
UCSCiuc007cqv.3. mouse. [Q6Q477-1]
uc007cqw.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY560895 mRNA. Translation: AAT01506.1.
AC124338 Genomic DNA. No translation available.
AC157924 Genomic DNA. No translation available.
BC109172 mRNA. Translation: AAI09173.1.
BC109173 mRNA. Translation: AAI09174.1.
CCDSiCCDS15298.1. [Q6Q477-1]
RefSeqiNP_001161421.1. NM_001167949.2.
NP_998781.1. NM_213616.4. [Q6Q477-1]
XP_006529786.1. XM_006529723.2. [Q6Q477-1]
XP_006529787.1. XM_006529724.3. [Q6Q477-1]
XP_006529788.1. XM_006529725.2. [Q6Q477-1]
UniGeneiMm.188617.

3D structure databases

ProteinModelPortaliQ6Q477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46143N.
IntActiQ6Q477. 1 interactor.
STRINGi10090.ENSMUSP00000119242.

PTM databases

iPTMnetiQ6Q477.
PhosphoSitePlusiQ6Q477.

Proteomic databases

PRIDEiQ6Q477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112264; ENSMUSP00000107883; ENSMUSG00000026463. [Q6Q477-2]
ENSMUST00000143567; ENSMUSP00000119242; ENSMUSG00000026463. [Q6Q477-1]
ENSMUST00000165602; ENSMUSP00000133187; ENSMUSG00000026463. [Q6Q477-1]
GeneIDi381290.
KEGGimmu:381290.
UCSCiuc007cqv.3. mouse. [Q6Q477-1]
uc007cqw.1. mouse.

Organism-specific databases

CTDi493.
MGIiMGI:88111. Atp2b4.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
KOiK05850.
OMAiIGDTHYH.
OrthoDBiEOG091G057D.
PhylomeDBiQ6Q477.
TreeFamiTF300330.

Enzyme and pathway databases

ReactomeiR-MMU-418359. Reduction of cytosolic Ca++ levels.
R-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ6Q477.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026463.
ExpressionAtlasiQ6Q477. baseline and differential.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR030319. ATP2B1/4.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF276. PTHR24093:SF276. 2 hits.
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT2B4_MOUSE
AccessioniPrimary (citable) accession number: Q6Q477
Secondary accession number(s): Q32ME1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 9, 2015
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.