Q6Q2Z6 (ACOT5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-coenzyme A thioesterase 5
Short name=Acyl-CoA thioesterase 5
EC=3.1.2.2

Alternative name(s):
Peroxisomal acyl-coenzyme A thioester hydrolase Ic
Short name=Peroxisomal acyl-CoA thioesterase Ic

Gene names

Name:

Acot5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Seems to be involved in intraperoxisomal regulation of acyl-CoA levels, but not CoASH levels. Mainly active on medium-chain acyl-CoAs. May have a function in termination of beta-oxydation of fatty acids. Ref.1
Catalytic activity	Palmitoyl-CoA + H2O = CoA + palmitate.
Subcellular location	Peroxisome.
Tissue specificity	Highly expressed in spleen, brain, testis and proximal and distal intestine; expressed at low level in the liver. Ref.1
Induction	In the liver and kidney, by peroxisome proliferator, via the peroxisome proliferator-activated receptors (PPARs) and by fasting.
Sequence similarities	Belongs to the C/M/P thioester hydrolase family.

Ontologies

Keywords
Cellular component	Peroxisome
Molecular function	Hydrolase Serine esterase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	acyl-CoA metabolic process Inferred from direct assay. Source: HGNC long-chain fatty acid metabolic process Inferred from direct assay. Source: HGNC saturated monocarboxylic acid metabolic process Inferred from direct assay. Source: HGNC unsaturated monocarboxylic acid metabolic process Inferred from direct assay. Source: HGNC very long chain fatty acid metabolic process Inferred from direct assay. Source: HGNC
Cellular component	cytosol Inferred from direct assay Ref.1. Source: MGI peroxisome Traceable author statement. Source: HGNC
Molecular function	carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW palmitoyl-CoA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Acyl-coenzyme A thioesterase 5		PRO_0000202151
Regions
	Motif	419 – 421	3	Microbody targeting signal Potential
Sites
	Active site	232	1	Charge relay system By similarity
	Active site	326	1	Charge relay system By similarity
	Active site	360	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	56	1	Phosphoserine Ref.3
Experimental info
	Sequence conflict	127	1	R → C in AAS75457. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6Q2Z6 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 3156BA777FFBEE94	FASTA	421	46,573
	10 20 30 40 50 60 MVPTVSLEPT GHSCWDEPLS IAVRGLAPEQ PVTLRTALRD EKGALFRAHA RYRADSHGEL 70 80 90 100 110 120 DLARTPALGG SFSGLEPMGL LWAMEPDRPF WRLIKRDVQT PFVVELEVLD GHEPDGGRLL 130 140 150 160 170 180 ARAVHERHFM APGVRRVPVR EGRVRATLFL PPGTGPFPGI IDLFGVGGGL LEYRASLLAG 190 200 210 220 230 240 KGFAVMALAY YKYDDLPKVI DILHLEYFEE AVTYLLSHPQ VKGPGVGLLG ISKGAELSLS 250 260 270 280 290 300 MASFLKGITA AVVINGATVN VISTLYYKEE SLPGLGMHLE RIKVTKDGFK DIIDILNVPL 310 320 330 340 350 360 EAPDQKSLIP LERSDTAFLF LVGQDDHNWK SEFYAREASK RLQAHGKEKP QIVCYPKTGH 370 380 390 400 410 420 HIEPPYIPWS IAAPHSYFDK PILLGGEPRA HAMAQVDAWQ RLQTFFHKHL SGDKRPSPAK L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of two mouse peroxisome proliferator-activated receptor alpha (PPARalpha)-regulated peroxisomal acyl-CoA thioesterases." Westin M.A.K., Alexson S.E.H., Hunt M.C. J. Biol. Chem. 279:21841-21848(2004) [PubMed: 15007068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: 129/Sv.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	"Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS." Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y. Mol. Cell. Proteomics 6:669-676(2007) [PubMed: 17208939] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY563099 mRNA. Translation: AAS75457.1. AC125071 Genomic DNA. No translation available.
IPI	IPI00130369.
RefSeq	NP_663419.3. NM_145444.3.
UniGene	Mm.484019.
3D structure databases
ProteinModelPortal	Q6Q2Z6.
SMR	Q6Q2Z6. Positions 1-410.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6Q2Z6.
Protein family/group databases
TCDB	4.C.3.1.1. acyl-CoA thioesterase (AcoT) family.
PTM databases
PhosphoSite	Q6Q2Z6.
Proteomic databases
PRIDE	Q6Q2Z6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000046422; ENSMUSP00000042019; ENSMUSG00000042540.
GeneID	217698.
KEGG	mmu:217698.
UCSC	uc007oeh.1. mouse.
Organism-specific databases
CTD	217698.
MGI	MGI:2384969. Acot5.
Phylogenomic databases
eggNOG	roNOG10401.
GeneTree	ENSGT00390000001046.
HOGENOM	HBG713807.
HOVERGEN	HBG000331.
InParanoid	Q6Q2Z6.
OrthoDB	EOG4QC15F.
Gene expression databases
ArrayExpress	Q6Q2Z6.
Bgee	Q6Q2Z6.
CleanEx	MM_ACOT5.
Genevestigator	Q6Q2Z6.
GermOnline	ENSMUSG00000042540. Mus musculus.
Family and domain databases
InterPro	IPR016662. Acyl-CoA_thioEstase_long-chain. IPR014940. BAAT_C. IPR006862. Thio_Ohase/aa_AcTrfase. [Graphical view]
KO	K01068.
Pfam	PF08840. BAAT_C. 1 hit. PF04775. Bile_Hydr_Trans. 1 hit. [Graphical view]
PIRSF	PIRSF016521. Acyl-CoA_hydro. 1 hit.
ProtoNet	Search...
Other
NextBio	375968.
SOURCE	Search...

Entry information

Entry name

ACOT5_MOUSE

Accession

Primary (citable) accession number: Q6Q2Z6
Secondary accession number(s): E9QKJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program