Reviewed, UniProtKB/Swiss-Prot Q6Q252 (PA11_POLDO)

Names and origin

Protein names	Recommended name:     Venom phospholipase A1 1     EC=3.1.1.32 Alternative name(s):     Allergen=Pol d 1.01
Organism	Polistes dominula (European paper wasp) (Vespa dominula)
Taxonomic identifier	34728 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Polistinae › Polistini › Polistes

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolysis of phosphatidylcholine with phospholipase A1 (EC 3.1.1.32) activity. Has weak hemolytic activity By similarity.
Catalytic activity	Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Contains six disulfide bonds By similarity.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis Lipid degradation
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis by symbiont of host erythrocytes Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phospholipase A1 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 337	316	Potential		PRO_5000093090
	Chain	36 – 337	302	Venom phospholipase A1 1		PRO_5000093091
Sites
	Active site	174	1	Nucleophile By similarity
	Active site	202	1	Charge relay system By similarity
	Active site	263	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6Q252-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C0A7E0E5EBDE448C	FASTA	337	37,559
	10 20 30 40 50 60 MNFKYSILFI CFVKVLDNCY AADDLTTLRN GTLDRGITPD CTFNEKDIEL HVYSRDKRNG 70 80 90 100 110 120 IILKKEILKN YDLFQKSQIS HQIAILIHGF LSTGNNENFD AMAKALIEID NFLVISVDWK 130 140 150 160 170 180 KGACNAFAST NDVLGYSQAV GNTRHVGKYV ADFTKLLVEQ YKVPMSNIRL IGHSLGAHTS 190 200 210 220 230 240 GFAGKEVQRL KLGKYKEIIG LDPAGPSFLT NKCPNRLCET DAEYVQAIHT SAILGVYYNV 250 260 270 280 290 300 GSVDFYVNYG KSQPGCSEPS CSHTKAVKYL TECIKRECCL IGTPWKSYFS TPKPISQCKR 310 320 330 DTCVCVGLNA QSYPAKGSFY VPVDKDAPYC HNEGIKL

References

[1]	Moawad T.I.S., Hoffman D.R. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY566645 mRNA. Translation: AAS67041.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.32. 288870.
Family and domain databases
InterPro	IPR002334. Dol/Ves_allerg. IPR000734. Lipase. IPR013818. Lipase_N. IPR008262. Lipase_Ser_AS. [Graphical view]
PANTHER	PTHR11610. Lipase. 1 hit.
Pfam	PF00151. Lipase. 1 hit. [Graphical view]
PRINTS	PR00825. DOLALLERGEN.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA11_POLDO

Accession

Primary (citable) accession number: Q6Q252

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	September 22, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)