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Protein

Cytosolic non-specific dipeptidase

Gene

Cndp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Catalyzes the production of N-lactoyl-amino acids from lactate and amino acids by reverse proteolysis.By similarity

Catalytic activityi

Hydrolysis of dipeptides, preferentially hydrophobic dipeptides including prolyl amino acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Manganese 2By similarity1
Active sitei101By similarity1
Metal bindingi132Manganese 1By similarity1
Metal bindingi132Manganese 2By similarity1
Active sitei166Proton acceptorBy similarity1
Metal bindingi167Manganese 1By similarity1
Metal bindingi195Manganese 2By similarity1
Binding sitei195Substrate; via carbonyl oxygenBy similarity1
Binding sitei228Substrate; shared with homodimeric partnerBy similarity1
Sitei228Important for catalytic activityBy similarity1
Binding sitei330Substrate; shared with homodimeric partnerBy similarity1
Binding sitei343SubstrateBy similarity1
Binding sitei417Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Metal bindingi445Manganese 1By similarity1
Binding sitei445SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-174403. Glutathione synthesis and recycling.

Protein family/group databases

MEROPSiM20.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic non-specific dipeptidase (EC:3.4.13.18)
Alternative name(s):
CNDP dipeptidase 2
Gene namesi
Name:Cndp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi1305827. Cndp2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002884961 – 475Cytosolic non-specific dipeptidaseAdd BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineCombined sources1
Modified residuei299PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6Q0N1.
PRIDEiQ6Q0N1.

PTM databases

iPTMnetiQ6Q0N1.
PhosphoSitePlusiQ6Q0N1.

Expressioni

Tissue specificityi

Detected in neuronal cells in the dorsal part of the tuberomammilary nucleus of the posterior hypothalamus (at protein level). Expressed in the iris, ciliary body, trabecular meshwork, Schlemm's canal, sclera, retina, brain, kidney and lung.2 Publications

Gene expression databases

BgeeiENSRNOG00000015591.
GenevisibleiQ6Q0N1. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021400.

Structurei

3D structure databases

ProteinModelPortaliQ6Q0N1.
SMRiQ6Q0N1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 167Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Phylogenomic databases

eggNOGiKOG2276. Eukaryota.
COG0624. LUCA.
GeneTreeiENSGT00390000009682.
HOGENOMiHOG000216709.
HOVERGENiHBG051103.
InParanoidiQ6Q0N1.
KOiK08660.
OMAiHITIPGF.
OrthoDBiEOG091G05RU.
PhylomeDBiQ6Q0N1.
TreeFamiTF300633.

Family and domain databases

CDDicd05676. M20_dipept_like_CNDP. 1 hit.
InterProiView protein in InterPro
IPR001261. ArgE/DapE_CS.
IPR017153. CNDP/DUG1.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
PfamiView protein in Pfam
PF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
PIRSFiPIRSF037242. CNDP_dipeptidase. 1 hit.
PROSITEiView protein in PROSITE
PS00759. ARGE_DAPE_CPG2_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6Q0N1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALKAVFQY IDENQDRFVK KLAEWVAIQS VSAWPEKRGE IRRMTEAAAA
60 70 80 90 100
DVQRLGGSVE LVDIGKQKLP DGSEIPLPPI LLGKLGSDPQ KKTVCIYGHL
110 120 130 140 150
DVQPAALEDG WDSEPFTLVE REGKLYGRGS TDDKGPVAGW MNALEAYQKT
160 170 180 190 200
GQEIPVNLRF CLEGMEESGS EGLDELIFAQ KDKFFKDVDY VCISDNYWLG
210 220 230 240 250
KNKPCITYGL RGICYFFIEV ECSDKDLHSG VYGGSVHEAM TDLISLMGCL
260 270 280 290 300
IDKKGKILIP GINDAVAPVT DEEHELYDHI DFDMEEFAKD VGAGTLLHSC
310 320 330 340 350
KKDILMHRWR YPSLSLHGIE GAFSGSGAKT VIPRKVVGKF SIRLVPDMIP
360 370 380 390 400
EVVSEQVSSY LSKKFAELQS PNKFKVYMGH GGKPWVSDFN HPHYQAGRRA
410 420 430 440 450
LKTVFGVEPD LTREGGSIPV TLTFQEATGK NVMLLPVGSA DDGAHSQNEK
460 470
LNRLNYIEGT KMLAAYLYEV SQLKN
Length:475
Mass (Da):52,693
Last modified:July 5, 2004 - v1
Checksum:i5B591868ABC02C55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY569013 mRNA. Translation: AAS75316.1.
BC095904 mRNA. Translation: AAH95904.1.
RefSeqiNP_001010920.1. NM_001010920.1.
XP_006255061.1. XM_006254999.3.
UniGeneiRn.98790.

Genome annotation databases

EnsembliENSRNOT00000021400; ENSRNOP00000021400; ENSRNOG00000015591.
GeneIDi291394.
KEGGirno:291394.

Similar proteinsi

Entry informationi

Entry nameiCNDP2_RAT
AccessioniPrimary (citable) accession number: Q6Q0N1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 5, 2004
Last modified: November 22, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families