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Protein

Cytosolic non-specific dipeptidase

Gene

Cndp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Catalyzes the production of N-lactoyl-amino acids from lactate and amino acids by reverse proteolysis.By similarity

Catalytic activityi

Hydrolysis of dipeptides, preferentially hydrophobic dipeptides including prolyl amino acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Manganese 2By similarity1
Active sitei101By similarity1
Metal bindingi132Manganese 1By similarity1
Metal bindingi132Manganese 2By similarity1
Active sitei166Proton acceptorBy similarity1
Metal bindingi167Manganese 1By similarity1
Metal bindingi195Manganese 2By similarity1
Binding sitei195Substrate; via carbonyl oxygenBy similarity1
Binding sitei228Substrate; shared with homodimeric partnerBy similarity1
Sitei228Important for catalytic activityBy similarity1
Binding sitei330Substrate; shared with homodimeric partnerBy similarity1
Binding sitei343SubstrateBy similarity1
Binding sitei417Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Metal bindingi445Manganese 1By similarity1
Binding sitei445SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1614635. Sulfur amino acid metabolism.
R-RNO-174403. Glutathione synthesis and recycling.

Protein family/group databases

MEROPSiM20.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic non-specific dipeptidase (EC:3.4.13.18)
Alternative name(s):
CNDP dipeptidase 2
Gene namesi
Name:Cndp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi1305827. Cndp2.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002884962 – 475Cytosolic non-specific dipeptidaseAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineCombined sources1
Modified residuei299PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6Q0N1.
PRIDEiQ6Q0N1.

PTM databases

iPTMnetiQ6Q0N1.
PhosphoSitePlusiQ6Q0N1.

Expressioni

Tissue specificityi

Detected in neuronal cells in the dorsal part of the tuberomammilary nucleus of the posterior hypothalamus (at protein level). Expressed in the iris, ciliary body, trabecular meshwork, Schlemm's canal, sclera, retina, brain, kidney and lung.2 Publications

Gene expression databases

BgeeiENSRNOG00000015591.
GenevisibleiQ6Q0N1. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021400.

Structurei

3D structure databases

ProteinModelPortaliQ6Q0N1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 167Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Phylogenomic databases

eggNOGiKOG2276. Eukaryota.
COG0624. LUCA.
GeneTreeiENSGT00390000009682.
HOGENOMiHOG000216709.
HOVERGENiHBG051103.
InParanoidiQ6Q0N1.
KOiK08660.
OMAiWSRPTCE.
OrthoDBiEOG091G05RU.
PhylomeDBiQ6Q0N1.
TreeFamiTF300633.

Family and domain databases

CDDicd05676. M20_dipept_like_CNDP. 1 hit.
Gene3Di3.30.70.360. 1 hit.
InterProiIPR001261. ArgE/DapE_CS.
IPR017153. CNDP/DUG1.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF037242. CNDP_dipeptidase. 1 hit.
PROSITEiPS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6Q0N1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALKAVFQY IDENQDRFVK KLAEWVAIQS VSAWPEKRGE IRRMTEAAAA
60 70 80 90 100
DVQRLGGSVE LVDIGKQKLP DGSEIPLPPI LLGKLGSDPQ KKTVCIYGHL
110 120 130 140 150
DVQPAALEDG WDSEPFTLVE REGKLYGRGS TDDKGPVAGW MNALEAYQKT
160 170 180 190 200
GQEIPVNLRF CLEGMEESGS EGLDELIFAQ KDKFFKDVDY VCISDNYWLG
210 220 230 240 250
KNKPCITYGL RGICYFFIEV ECSDKDLHSG VYGGSVHEAM TDLISLMGCL
260 270 280 290 300
IDKKGKILIP GINDAVAPVT DEEHELYDHI DFDMEEFAKD VGAGTLLHSC
310 320 330 340 350
KKDILMHRWR YPSLSLHGIE GAFSGSGAKT VIPRKVVGKF SIRLVPDMIP
360 370 380 390 400
EVVSEQVSSY LSKKFAELQS PNKFKVYMGH GGKPWVSDFN HPHYQAGRRA
410 420 430 440 450
LKTVFGVEPD LTREGGSIPV TLTFQEATGK NVMLLPVGSA DDGAHSQNEK
460 470
LNRLNYIEGT KMLAAYLYEV SQLKN
Length:475
Mass (Da):52,693
Last modified:July 5, 2004 - v1
Checksum:i5B591868ABC02C55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY569013 mRNA. Translation: AAS75316.1.
BC095904 mRNA. Translation: AAH95904.1.
RefSeqiNP_001010920.1. NM_001010920.1.
XP_006255061.1. XM_006254999.3.
UniGeneiRn.98790.

Genome annotation databases

EnsembliENSRNOT00000021400; ENSRNOP00000021400; ENSRNOG00000015591.
GeneIDi291394.
KEGGirno:291394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY569013 mRNA. Translation: AAS75316.1.
BC095904 mRNA. Translation: AAH95904.1.
RefSeqiNP_001010920.1. NM_001010920.1.
XP_006255061.1. XM_006254999.3.
UniGeneiRn.98790.

3D structure databases

ProteinModelPortaliQ6Q0N1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021400.

Protein family/group databases

MEROPSiM20.005.

PTM databases

iPTMnetiQ6Q0N1.
PhosphoSitePlusiQ6Q0N1.

Proteomic databases

PaxDbiQ6Q0N1.
PRIDEiQ6Q0N1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021400; ENSRNOP00000021400; ENSRNOG00000015591.
GeneIDi291394.
KEGGirno:291394.

Organism-specific databases

CTDi55748.
RGDi1305827. Cndp2.

Phylogenomic databases

eggNOGiKOG2276. Eukaryota.
COG0624. LUCA.
GeneTreeiENSGT00390000009682.
HOGENOMiHOG000216709.
HOVERGENiHBG051103.
InParanoidiQ6Q0N1.
KOiK08660.
OMAiWSRPTCE.
OrthoDBiEOG091G05RU.
PhylomeDBiQ6Q0N1.
TreeFamiTF300633.

Enzyme and pathway databases

ReactomeiR-RNO-1614635. Sulfur amino acid metabolism.
R-RNO-174403. Glutathione synthesis and recycling.

Miscellaneous databases

PROiQ6Q0N1.

Gene expression databases

BgeeiENSRNOG00000015591.
GenevisibleiQ6Q0N1. RN.

Family and domain databases

CDDicd05676. M20_dipept_like_CNDP. 1 hit.
Gene3Di3.30.70.360. 1 hit.
InterProiIPR001261. ArgE/DapE_CS.
IPR017153. CNDP/DUG1.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF037242. CNDP_dipeptidase. 1 hit.
PROSITEiPS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNDP2_RAT
AccessioniPrimary (citable) accession number: Q6Q0N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.