Reviewed, UniProtKB/Swiss-Prot Q6Q0C0 (TRAF7_HUMAN)

Names and origin

Protein names

Recommended name:
    E3 ubiquitin-protein ligase TRAF7
    EC=6.3.2.-
Alternative name(s):
    TNF receptor-associated factor 7
    RING finger and WD repeat-containing protein 1
    RING finger protein 119

Gene names

Name:	TRAF7
Synonyms:	RFWD1, RNF119

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	670 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	E3 ubiquitin ligase capable of auto-ubiquitination, following phosphorylation by MAP3K3. Potentiates MEKK3-mediated activation of the NF-kappa-B, JUN/AP1 and DDIT3 transcriptional regulators. Induces apoptosis when overexpressed. Ref.1 Ref.4
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Homodimer. Interacts with MAP3K3 and promotes the kinase activity of this enzyme. Ref.1 Ref.4
Subcellular location	Cytoplasmic vesicle. Note: Colocalizes with MAP3K3 to vesicle-like structures throughout the cytoplasm. Ref.4
Tissue specificity	Ubiquitously expressed with high levels in skeletal muscle, heart, colon, spleen, kidney, liver and placenta. Ref.1
Post-translational modification	Phosphorylated by MAP3K3. Ref.4 Ref.5 Ubiquitinates itself upon phosphorylation.
Sequence similarities	Contains 1 RING-type zinc finger. Contains 1 TRAF-type zinc finger. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Apoptosis Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Cytoplasmic vesicle
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	activation of MAPKKK activity Ref.4 Inferred from direct assay. Source: UniProtKB apoptosis Ref.1 Inferred from direct assay. Source: MGI modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of MAPKKK cascade Ref.1 Inferred from direct assay. Source: MGI protein ubiquitination Ref.4 Inferred from direct assay. Source: UniProtKB regulation of apoptosis Ref.1 Inferred from mutant phenotype. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell ubiquitin ligase complex Ref.4 Inferred from direct assay. Source: UniProtKB
Molecular function	identical protein binding Ref.4 Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Ref.4 Inferred from direct assay. Source: UniProtKB zinc ion binding Ref.4 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-307556,EBI-307556
MAP3K3	Q99759	2	EBI-307556,EBI-307281

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q6Q0C0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: Q6Q0C0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 670	670	E3 ubiquitin-protein ligase TRAF7		PRO_0000051296
Regions
	Repeat	394 – 433	40	WD 1
	Repeat	437 – 474	38	WD 2
	Repeat	477 – 513	37	WD 3
	Repeat	515 – 554	40	WD 4
	Repeat	557 – 594	38	WD 5
	Repeat	597 – 638	42	WD 6
	Repeat	641 – 669	29	WD 7
	Zinc finger	131 – 165	35	RING-type
	Zinc finger	222 – 276	55	TRAF-type
Amino acid modifications
	Modified residue	88	1	Phosphoserine Ref.5
	Modified residue	91	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	1 – 76	76	Missing in isoform 2. Ref.1		VSP_051607

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5624045D674849C6	FASTA	670	74,609
	10 20 30 40 50 60 MSSGKSARYN RFSGGPSNLP TPDVTTGTRM ETTFGPAFSA VTTITKADGT STYKQHCRTP 70 80 90 100 110 120 SSSSTLAYSP RDEEDSMPPI STPRRSDSAI SVRSLHSESS MSLRSTFSLP EEEEEPEPLV 130 140 150 160 170 180 FAEQPSVKLC CQLCCSVFKD PVITTCGHTF CRRCALKSEK CPVDNVKLTV VVNNIAVAEQ 190 200 210 220 230 240 IGELFIHCRH GCRVAGSGKP PIFEVDPRGC PFTIKLSARK DHEGSCDYRP VRCPNNPSCP 250 260 270 280 290 300 PLLRMNLEAH LKECEHIKCP HSKYGCTFIG NQDTYETHLE TCRFEGLKEF LQQTDDRFHE 310 320 330 340 350 360 MHVALAQKDQ EIAFLRSMLG KLSEKIDQLE KSLELKFDVL DENQSKLSED LMEFRRDASM 370 380 390 400 410 420 LNDELSHINA RLNMGILGSY DPQQIFKCKG TFVGHQGPVW CLCVYSMGDL LFSGSSDKTI 430 440 450 460 470 480 KVWDTCTTYK CQKTLEGHDG IVLALCIQGC KLYSGSADCT IIVWDIQNLQ KVNTIRAHDN 490 500 510 520 530 540 PVCTLVSSHN VLFSGSLKAI KVWDIVGTEL KLKKELTGLN HWVRALVAAQ SYLYSGSYQT 550 560 570 580 590 600 IKIWDIRTLD CIHVLQTSGG SVYSIAVTNH HIVCGTYENL IHVWDIESKE QVRTLTGHVG 610 620 630 640 650 660 TVYALAVIST PDQTKVFSAS YDRSLRVWSM DNMICTQTLL RHQGSVTALA VSRGRLFSGA 670 VDSTVKVWTC
	Isoform 2. Checksum: 25F8742FBD322058	FASTA	594	66,541
	10 20 30 40 50 60 MPPISTPRRS DSAISVRSLH SESSMSLRST FSLPEEEEEP EPLVFAEQPS VKLCCQLCCS 70 80 90 100 110 120 VFKDPVITTC GHTFCRRCAL KSEKCPVDNV KLTVVVNNIA VAEQIGELFI HCRHGCRVAG 130 140 150 160 170 180 SGKPPIFEVD PRGCPFTIKL SARKDHEGSC DYRPVRCPNN PSCPPLLRMN LEAHLKECEH 190 200 210 220 230 240 IKCPHSKYGC TFIGNQDTYE THLETCRFEG LKEFLQQTDD RFHEMHVALA QKDQEIAFLR 250 260 270 280 290 300 SMLGKLSEKI DQLEKSLELK FDVLDENQSK LSEDLMEFRR DASMLNDELS HINARLNMGI 310 320 330 340 350 360 LGSYDPQQIF KCKGTFVGHQ GPVWCLCVYS MGDLLFSGSS DKTIKVWDTC TTYKCQKTLE 370 380 390 400 410 420 GHDGIVLALC IQGCKLYSGS ADCTIIVWDI QNLQKVNTIR AHDNPVCTLV SSHNVLFSGS 430 440 450 460 470 480 LKAIKVWDIV GTELKLKKEL TGLNHWVRAL VAAQSYLYSG SYQTIKIWDI RTLDCIHVLQ 490 500 510 520 530 540 TSGGSVYSIA VTNHHIVCGT YENLIHVWDI ESKEQVRTLT GHVGTVYALA VISTPDQTKV 550 560 570 580 590 FSASYDRSLR VWSMDNMICT QTLLRHQGSV TALAVSRGRL FSGAVDSTVK VWTC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TRAF7 potentiates MEKK3-induced AP1 and CHOP activation and induces apoptosis." Xu L.-G., Li L.-Y., Shu H.-B. J. Biol. Chem. 279:17278-17282(2004) [PubMed: 15001576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING, INTERACTION WITH MAP3K3.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Liver.
[4]	"A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway." Bouwmeester T., Bauch A., Ruffner H., Angrand P.-O., Bergamini G., Croughton K., Cruciat C., Eberhard D., Gagneur J., Ghidelli S., Hopf C., Huhse B., Mangano R., Michon A.-M., Schirle M., Schlegl J., Schwab M., Stein M.A. , Bauer A., Casari G., Drewes G., Gavin A.-C., Jackson D.B., Joberty G., Neubauer G., Rick J., Kuster B., Superti-Furga G. Nat. Cell Biol. 6:97-105(2004) [PubMed: 14743216] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, HOMODIMERIZATION, INTERACTION WITH MAP3K3.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-88 AND SER-91, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY569455 mRNA. Translation: AAS68363.1. AL136921 mRNA. Translation: CAB66855.1. BC024267 mRNA. Translation: AAH24267.1.
IPI	IPI00395631. IPI00470778.
RefSeq	NP_115647.2.
UniGene	Hs.713632
3D structure databases
HSSP	HSSP built from PDB template 1ERJ based on UniProtKB P16649.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6Q0C0. 2 interactions.
PTM databases
PhosphoSite	Q6Q0C0.
Proteomic databases
PRIDE	Q6Q0C0.
Genome annotation databases
Ensembl	ENSG00000131653. Homo sapiens. [Contig view]
GeneID	84231.
KEGG	hsa:84231.
Organism-specific databases
GeneCards	GC16P002146.
H-InvDB	HIX0012714.
HGNC	HGNC:20456. TRAF7.
MIM	606692. gene.
PharmGKB	PA134917323.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q6Q0C0.
HOVERGEN	Q6Q0C0.
OMA	Q6Q0C0. MNLEAHL.
Gene expression databases
Bgee	Q6Q0C0.
CleanEx	HS_TRAF7.
GermOnline	ENSG00000131653. Homo sapiens.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like. IPR001680. WD40_repeat. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_region. IPR019781. WD40_repeat_sg. IPR018957. Znf_C3HC4_RING-type. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. IPR001293. Znf_TRAF. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 7 hits. PF00097. zf-C3HC4. 1 hit. PF02176. zf-TRAF. 1 hit. [Graphical view]
ProDom	PD000018. WD40. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00184. RING. 1 hit. SM00320. WD40. 7 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. PS50145. ZF_TRAF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	73692.
SOURCE	Search...

Entry information

Entry name

TRAF7_HUMAN

Accession

Primary (citable) accession number: Q6Q0C0
Secondary accession number(s): Q9H073

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)