Reviewed, UniProtKB/Swiss-Prot Q6PY58 (LEU3_CANMI)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
      Short name=3-IPM-DH
      Short name=IMDH
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase

Gene names

Name:

LEU2

Organism

Candida milleri (Sour dough yeast)

Taxonomic identifier

51915 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	3-isopropylmalate dehydrogenase		PRO_0000083605
Regions
	Nucleotide binding	79 – 90	12	NAD By similarity
	Nucleotide binding	289 – 300	12	NAD By similarity
Sites
	Metal binding	225	1	Magnesium or manganese By similarity
	Metal binding	250	1	Magnesium or manganese By similarity
	Metal binding	254	1	Magnesium or manganese By similarity
	Binding site	97	1	Substrate By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	225	1	Substrate By similarity
	Site	143	1	Important for catalysis By similarity
	Site	192	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6PY58-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 69C4AA375FE444DE	FASTA	363	39,017
	10 20 30 40 50 60 MSTTKTIVVL PGDHVGTEIT EEATKVLNAI AEKRPNIKFD FQHHLIGGAA IDATGVPLPD 70 80 90 100 110 120 EALEASKKAD AVLLGAVGGP KWGTGDVRPE QGLLKIRKEL GLYANLRPCN FAFDSLLDMS 130 140 150 160 170 180 PLKPEYARGT DFTVVRELVC GIYFGKRKED TGDGVTWDSE QYSVPEVQRI TRMAASLALQ 190 200 210 220 230 240 HNPPLPIWSL DKANVLASSR LWRKTVEETI KNEFPTLSVQ HQLIDSAAMI LVKNPTKLNG 250 260 270 280 290 300 LVITSNMFGD IISDEASVIP GSLGLLPSAS LASPPDTNKA FGLYEPCHGS APDLPKGKVN 310 320 330 340 350 360 PVATILSVAM MLKLSLDMVE EGIAVEKAVR KVIDNGIRTA DLRGTNSTTE VGDAIAAAVK EFL

References

[1]	"Cloning, sequencing and use as a chromosomal probe of the LEU2 gene from the sour dough yeast Candida milleri." Turakainen H., Korhola M. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CBS 8195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY571337 Genomic DNA. Translation: AAS77418.1.
3D structure databases
SMR	Q6PY58. Positions 6-359.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.85. 288940.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004429. Isopropylmalate_DH. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_CANMI

Accession

Primary (citable) accession number: Q6PY58

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)