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Protein

Complexin-2

Gene

CPLX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in synaptic vesicle exocytosis. Also involved in mast cell exocytosis (By similarity).By similarity

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. mast cell degranulation Source: UniProtKB-KW
  3. nervous system development Source: UniProtKB-KW
  4. positive regulation of synaptic plasticity Source: UniProtKB
  5. regulation of exocytosis Source: ParkinsonsUK-UCL
  6. synaptic vesicle exocytosis Source: Ensembl
  7. vesicle docking involved in exocytosis Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Differentiation, Exocytosis, Mast cell degranulation, Neurogenesis, Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Complexin-2
Alternative name(s):
Complexin II
Short name:
CPX II
Synaphin-1
Gene namesi
Name:CPLX2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:2310. CPLX2.

Subcellular locationi

  1. Cytoplasmcytosol

  2. Note: Enriched at synaptic-releasing sites in mature neurons.By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. dendrite Source: Ensembl
  3. mast cell granule Source: GOC
  4. neuronal cell body Source: Ensembl
  5. synapse Source: Ensembl
  6. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: Ensembl
  7. synaptobrevin 2-SNAP-25-syntaxin-3-complexin complex Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26827.

Polymorphism and mutation databases

BioMutaiCPLX2.
DMDMi51316998.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 134134Complexin-2PRO_0000144875Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei93 – 931PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PUV4.
PaxDbiQ6PUV4.
PeptideAtlasiQ6PUV4.
PRIDEiQ6PUV4.

PTM databases

PhosphoSiteiQ6PUV4.

Expressioni

Tissue specificityi

Nervous system. In hippocampus and cerebellum, expressed mainly by excitatory neurons. Down-regulated in brain cortex from patients suffering from Huntington disease, bipolar disorder or major depression. Down-regulated in cerebellum from patients with schizophrenia.5 Publications

Gene expression databases

BgeeiQ6PUV4.
CleanExiHS_CPLX2.
ExpressionAtlasiQ6PUV4. baseline and differential.
GenevestigatoriQ6PUV4.

Interactioni

Subunit structurei

Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.By similarity

Protein-protein interaction databases

BioGridi116027. 2 interactions.
IntActiQ6PUV4. 2 interactions.
STRINGi9606.ENSP00000352544.

Structurei

3D structure databases

ProteinModelPortaliQ6PUV4.
SMRiQ6PUV4. Positions 32-72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 9757Interaction with the SNARE complexBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili28 – 8457Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the complexin/synaphin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG85303.
GeneTreeiENSGT00490000043416.
HOVERGENiHBG062792.
InParanoidiQ6PUV4.
KOiK15294.
OMAiAMEQPCE.
OrthoDBiEOG71G9XG.
PhylomeDBiQ6PUV4.
TreeFamiTF315172.

Family and domain databases

InterProiIPR008849. Synaphin.
[Graphical view]
PANTHERiPTHR16705. PTHR16705. 1 hit.
PfamiPF05835. Synaphin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PUV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFVMKQALG GATKDMGKML GGEEEKDPDA QKKEEERQEA LRQQEEERKA
60 70 80 90 100
KHARMEAERE KVRQQIRDKY GLKKKEEKEA EEKAALEQPC EGSLTRPKKA
110 120 130
IPAGCGDEEE EEEESILDTV LKYLPGPLQD MFKK
Length:134
Mass (Da):15,394
Last modified:August 16, 2004 - v2
Checksum:i7791812FD4194AC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35100 mRNA. Translation: AAC50229.1.
AK314177 mRNA. Translation: BAG36859.1.
BC093706 mRNA. Translation: AAH93706.1.
BC112287 mRNA. Translation: AAI12288.1.
AY576870 mRNA. Translation: AAS93622.1.
BN000499 mRNA. Translation: CAG26663.1.
BN000500 mRNA. Translation: CAG26664.1.
CCDSiCCDS4396.1.
PIRiE57233.
RefSeqiNP_001008221.1. NM_001008220.1.
NP_006641.1. NM_006650.3.
XP_005265855.1. XM_005265798.1.
XP_005265856.1. XM_005265799.1.
UniGeneiHs.193235.

Genome annotation databases

EnsembliENST00000359546; ENSP00000352544; ENSG00000145920.
ENST00000393745; ENSP00000377346; ENSG00000145920.
ENST00000515094; ENSP00000421825; ENSG00000145920.
GeneIDi10814.
KEGGihsa:10814.
UCSCiuc003mde.1. human.

Polymorphism and mutation databases

BioMutaiCPLX2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35100 mRNA. Translation: AAC50229.1.
AK314177 mRNA. Translation: BAG36859.1.
BC093706 mRNA. Translation: AAH93706.1.
BC112287 mRNA. Translation: AAI12288.1.
AY576870 mRNA. Translation: AAS93622.1.
BN000499 mRNA. Translation: CAG26663.1.
BN000500 mRNA. Translation: CAG26664.1.
CCDSiCCDS4396.1.
PIRiE57233.
RefSeqiNP_001008221.1. NM_001008220.1.
NP_006641.1. NM_006650.3.
XP_005265855.1. XM_005265798.1.
XP_005265856.1. XM_005265799.1.
UniGeneiHs.193235.

3D structure databases

ProteinModelPortaliQ6PUV4.
SMRiQ6PUV4. Positions 32-72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116027. 2 interactions.
IntActiQ6PUV4. 2 interactions.
STRINGi9606.ENSP00000352544.

PTM databases

PhosphoSiteiQ6PUV4.

Polymorphism and mutation databases

BioMutaiCPLX2.
DMDMi51316998.

Proteomic databases

MaxQBiQ6PUV4.
PaxDbiQ6PUV4.
PeptideAtlasiQ6PUV4.
PRIDEiQ6PUV4.

Protocols and materials databases

DNASUi10814.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359546; ENSP00000352544; ENSG00000145920.
ENST00000393745; ENSP00000377346; ENSG00000145920.
ENST00000515094; ENSP00000421825; ENSG00000145920.
GeneIDi10814.
KEGGihsa:10814.
UCSCiuc003mde.1. human.

Organism-specific databases

CTDi10814.
GeneCardsiGC05P175156.
HGNCiHGNC:2310. CPLX2.
MIMi605033. gene.
neXtProtiNX_Q6PUV4.
PharmGKBiPA26827.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG85303.
GeneTreeiENSGT00490000043416.
HOVERGENiHBG062792.
InParanoidiQ6PUV4.
KOiK15294.
OMAiAMEQPCE.
OrthoDBiEOG71G9XG.
PhylomeDBiQ6PUV4.
TreeFamiTF315172.

Miscellaneous databases

ChiTaRSiCPLX2. human.
GeneWikiiCPLX2.
GenomeRNAii10814.
NextBioi20805633.
PROiQ6PUV4.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PUV4.
CleanExiHS_CPLX2.
ExpressionAtlasiQ6PUV4. baseline and differential.
GenevestigatoriQ6PUV4.

Family and domain databases

InterProiIPR008849. Synaphin.
[Graphical view]
PANTHERiPTHR16705. PTHR16705. 1 hit.
PfamiPF05835. Synaphin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complexins: cytosolic proteins that regulate SNAP receptor function."
    McMahon H.T., Missler M., Li C., Suedhof T.C.
    Cell 83:111-119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cerebellum.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Structural organization of the human complexin 2 gene (CPLX2) and aspects of its functional activity."
    Raevskaya N.M., Dergunova L.V., Vladychenskaya I.P., Stavchansky V.V., Oborina M.V., Poltaraus A.B., Limborska S.A.
    Gene 359:127-137(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-114, TISSUE SPECIFICITY.
    Tissue: Cerebellum.
  5. "Preferential involvement of excitatory neurons in medial temporal lobe in schizophrenia."
    Harrison P.J., Eastwood S.L.
    Lancet 352:1669-1673(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Synaptic pathology in the anterior cingulate cortex in schizophrenia and mood disorders. A review and a Western blot study of synaptophysin, GAP-43 and the complexins."
    Eastwood S.L., Harrison P.J.
    Brain Res. Bull. 55:569-578(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Cerebellar synaptic protein expression in schizophrenia."
    Eastwood S.L., Cotter D., Harrison P.J.
    Neuroscience 105:219-229(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Early changes in Huntington's disease patient brains involve alterations in cytoskeletal and synaptic elements."
    DiProspero N.A., Chen E.-Y., Charles V., Plomann M., Kordower J.H., Tagle D.A.
    J. Neurocytol. 33:517-533(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCPLX2_HUMAN
AccessioniPrimary (citable) accession number: Q6PUV4
Secondary accession number(s): B2RAG2
, O09056, Q13329, Q28184, Q52M15, Q64386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: April 29, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.