Reviewed, UniProtKB/Swiss-Prot Q6PSU2 (CONG7_ARAHY)

Names and origin

Protein names	Recommended name:     Conglutin-7 Alternative name(s):     Seed storage protein SSP1     Seed storage protein SSP2     2S protein 1     Allergen=Ara h 2.02
Organism	Arachis hypogaea (Peanut)
Taxonomic identifier	3818 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Dalbergieae › Arachis

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Weak inhibitor of trypsin. Ref.12
Tissue specificity	Expressed in seeds, not expressed in leaves, roots and pegs. Ref.8
Developmental stage	Expressed at very low levels in immature seeds and at high levels from 40-75 days after pollination. Expression decreases after 75 days after pollination. Ref.8
Induction	Repressed by water stress. Ref.10
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. Ref.11
Miscellaneous	Resistant to proteolysis. Ref.11
Sequence similarities	Belongs to the 2S seed storage albumins family.
Biophysicochemical properties	Temperature dependence: Thermostable. Ref.11
Mass spectrometry	Molecular mass is 18050 Da from positions 22 - 172. Determined by MALDI. Ref.1 Molecular mass is 16670 Da from positions 22 - 172. Determined by MALDI. Variant 76-Gln--Ser-87 Del. Ref.1

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Disease	Allergen
Domain	Signal
Ligand	IgE-binding protein
Molecular function	Protease inhibitor Seed storage protein Serine protease inhibitor Storage protein
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	IgE binding Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.1 Ref.10
	Chain	22 – 172	151	Conglutin-7 Ref.1 Ref.10		PRO_0000370687
Amino acid modifications
	Disulfide bond	33 ↔ 116		By similarity UniProtKB Q647G9
	Disulfide bond	45 ↔ 103		By similarity UniProtKB Q647G9
	Disulfide bond	104 ↔ 152		By similarity UniProtKB Q647G9
	Disulfide bond	118 ↔ 160		By similarity UniProtKB Q647G9
Natural variations
	Natural variant	76 – 87	12	Missing in short form. Ref.2 Ref.4 Ref.7
Experimental info
	Sequence conflict	10	1	L → P in AAM78596. Ref.9
	Sequence conflict	27	1	L → F in AAT00599. Ref.4
	Sequence conflict	61	1	G → E in AAU21494. Ref.2
	Sequence conflict	61	1	G → E in AAT00599. Ref.4
	Sequence conflict	61	1	G → E in AAK96887. Ref.7
	Sequence conflict	65 – 78	14	Missing in ABL14268. Ref.5
	Sequence conflict	163	1	E → D in AAU21494. Ref.2
	Sequence conflict	163	1	E → D in AAT00599. Ref.4
	Sequence conflict	163	1	E → D in AAK96887. Ref.7
	Sequence conflict	163	1	E → D AA sequence Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q6PSU2-1 [UniParc]. Last modified May 15, 2007. Version 2. Checksum: B8BB91C8D8C143AB	FASTA	172	20,114
	10 20 30 40 50 60 MAKLTILVAL ALFLLAAHAS ARQQWELQGD RRCQSQLERA NLRPCEQHLM QKIQRDEDSY 70 80 90 100 110 120 GRDPYSPSQD PYSPSQDPDR RDPYSPSPYD RRGAGSSQHQ ERCCNELNEF ENNQRCMCEA 130 140 150 160 170 LQQIMENQSD RLQGRQQEQQ FKRELRNLPQ QCGLRAPQRC DLEVESGGRD RY

References

[1]	"Isolation and characterization of two complete Ara h 2 isoforms cDNA." Chatel J.-M., Bernard H., Orson F.M. Int. Arch. Allergy Immunol. 131:14-18(2003) [PubMed: 12759484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-31, MASS SPECTROMETRY.
[2]	"Isolation of peanut genes encoding arachins and conglutins by expressed sequence tags." Yan Y.-S., Lin X.-D., Zhang Y.-S., Wang L., Wu K., Huang S.-Z. Plant Sci. 169:439-445(2005) [Agricola: IND43739496] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT 76-GLN--SER-87 DEL. Strain: cv. Shanyou 523. Tissue: Cotyledon.
[3]	"Chromosomal and phylogenetic context for conglutin genes in Arachis based on genomic sequence." Ramos M.L., Fleming G., Chu Y., Akiyama Y., Gallo M., Ozias-Akins P. Mol. Genet. Genomics 275:578-592(2006) [PubMed: 16614814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. F78-1339.
[4]	"cDNA cloning of peanut seed storage protein." Yan Y.-S., Wang L., Liao B., Li H., Lin X.-D., Huang S.-Z. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT 76-GLN--SER-87 DEL. Strain: cv. Shanyou 523.
[5]	"Isolation of peanut genes encoding seed storage proteins and stress proteins from developing cotyledons by expressed sequence tags." Fu G., Yan Y.-S., Wang L., Zhong Y., Huang S.-Z. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Shanyou 523.
[6]	"Cloning and characterization of four genes encoding peanut seed oleosins." Li C., Fu G., Zhong Y., Yan Y., Wang L., Huang S. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Shanyou 523.
[7]	"Isolation and molecular characterization of the first genomic clone of a major peanut allergen, Ara h 2." Viquez O.M., Summer C.G., Dodo H.W. J. Allergy Clin. Immunol. 107:713-717(2001) [PubMed: 11295663] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-168, VARIANT 76-GLN--SER-87 DEL. Strain: cv. F78-1339. Tissue: Seed.
[8]	"Seed-specific, developmentally regulated genes of peanut." Paik-Ro O.G., Seib J.C., Smith R.L. Theor. Appl. Genet. 104:236-240(2002) [PubMed: 12582692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-168, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. FL435. Tissue: Seed.
[9]	"Re-investigation of the major peanut allergen Arah2 on the molecular level." Becker W.-M., Suhr M., Lindner B., Wicklein D., Lepp U. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-172.
[10]	"Suppression of seed storage proteins upon water stress in Arachis hypogea var. M-13 seeds." Katam R., Vasanthaiah H.K.N., Basha S.M., McClung S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 22-33; 117-131; 147-155 AND 160-169, REPRESSION BY WATER STRESS. Strain: cv. M13. Tissue: Seed.
[11]	"Structure and stability of 2S albumin-type peanut allergens: implications for the severity of peanut allergic reactions." Lehmann K., Schweimer K., Reese G., Randow S., Suhr M., Becker W.-M., Vieths S., Roesch P. Biochem. J. 395:463-472(2006) [PubMed: 16372900] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-31 AND 93-99, ALLERGENICITY, RESISTANCE TO HEAT AND PROTEOLYSIS.
[12]	"The major peanut allergen, Ara h 2, functions as a trypsin inhibitor, and roasting enhances this function." Maleki S.J., Viquez O.M., Jacks T., Dodo H.W., Champagne E.T., Chung S.-Y., Landry S.J. J. Allergy Clin. Immunol. 112:190-195(2003) [PubMed: 12847498] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY158467 mRNA. Translation: AAN77576.1. AY581853 mRNA. Translation: AAT00598.1. Different initiation. AY722689 mRNA. Translation: AAU21494.1. Different initiation. EF609644 Genomic DNA. Translation: ABQ96215.1. AY581854 mRNA. Translation: AAT00599.1. Different initiation. EF080817 mRNA. Translation: ABL14268.1. EF695402 Genomic DNA. Translation: ABS28872.1. AY007229 Genomic DNA. Translation: AAK96887.1. AF366560 mRNA. Translation: AAO61750.1. AY117434 mRNA. Translation: AAM78596.1.
3D structure databases
HSSP	HSSP built from PDB template 1W2Q based on UniProtKB Q647G9.
SMR	Q6PSU2. Positions 59-182.
ModBase	Search...
Family and domain databases
InterPro	IPR016140. Bifunc_inhib/LTP/seed_store. IPR003612. LTP/seed_store/tryp_amyl_inhib. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Gene3D	G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
SMART	SM00499. AAI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CONG7_ARAHY

Accession

Primary (citable) accession number: Q6PSU2
Secondary accession number(s): A1DZE8 , A5Z1R1, Q647H0, Q6PSU1, Q7Y1C0, Q84TU1, Q8GV20, Q941R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	November 24, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)