Conglutin-7 precursor - Arachis hypogaea (Peanut)

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Q6PSU2 (CONG7_ARAHY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 8, 2011. Version 34. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names	Recommended name: Conglutin-7 Alternative name(s): 2S protein 1 Seed storage protein SSP1 Seed storage protein SSP2 Allergen=Ara h 2
Organism	Arachis hypogaea (Peanut)
Taxonomic identifier	3818 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Dalbergieae › Arachis

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Weak inhibitor of trypsin. Ref.14
Tissue specificity	Expressed in seeds, not expressed in leaves, roots and pegs. Ref.9
Developmental stage	Expressed at very low levels in immature seeds and at high levels from 40-75 days after pollination. Expression decreases after 75 days after pollination. Ref.9
Induction	Repressed by water stress. Ref.10
Post-translational modification	The hydroxyproline modifications determined by mass spectrometry (Ref.11) are probably 4-hydroxyproline as determined for other extracellular plant proteins.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. Ref.13
Miscellaneous	Resistant to proteolysis. Ref.13
Sequence similarities	Belongs to the 2S seed storage albumins family.
Biophysicochemical properties	Temperature dependence: Thermostable. Ref.13
Mass spectrometry	Molecular mass is 18050 Da from positions 22 - 172. Determined by MALDI. Isoform 1. Ref.1 Molecular mass is 16670 Da from positions 22 - 172. Determined by MALDI. Isoform 3. Ref.1
Sequence caution	The sequence AAT00598.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAT00599.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAU21494.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Disease	Allergen
Domain	Signal
Ligand	IgE-binding protein
Molecular function	Protease inhibitor Seed storage protein Serine protease inhibitor Storage protein
PTM	Disulfide bond Hydroxylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	IgE binding Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6PSU2-1) Also known as: P1; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6PSU2-2) Also known as: P2; The sequence of this isoform differs from the canonical sequence as follows: 76-87: Missing.

Isoform 3 (identifier: Q6PSU2-3) Also known as: P3; The sequence of this isoform differs from the canonical sequence as follows: 170-172: DRY → D

Isoform 4 (identifier: Q6PSU2-4) Also known as: P4; The sequence of this isoform differs from the canonical sequence as follows: 76-87: Missing. 170-172: DRY → D

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

21

Ref.1 Ref.10 Ref.11 Ref.12

Chain

151

Conglutin-7 Ref.1 Ref.10

PRO_0000370687

Amino acid modifications

Modified residue

1

4-hydroxyproline Ref.11

Modified residue

1

4-hydroxyproline Ref.11

Modified residue

1

4-hydroxyproline Ref.11

Disulfide bond

Ref.11 UniProtKB Q647G9

Disulfide bond

Or C-45 with C-104 Ref.11 UniProtKB Q647G9

Disulfide bond

Or C-103 with C-152 Ref.11 UniProtKB Q647G9

Disulfide bond

Ref.11 UniProtKB Q647G9

Natural variations

Alternative sequence

12

Missing in isoform 2 and isoform 4.

VSP_038916

Alternative sequence

3

DRY → D in isoform 3 and isoform 4.

VSP_038917

Experimental info

Sequence conflict

2

Missing in ACN62248. Ref.7

Sequence conflict

1

L → P in AAM78596. Ref.10

Sequence conflict

1

L → F in AAT00599. Ref.4

Sequence conflict

1

G → E in AAU21494. Ref.2

Sequence conflict

1

G → E in AAT00599. Ref.4

Sequence conflict

1

G → E in ACN62248. Ref.7

Sequence conflict

1

G → E in AAK96887. Ref.8

Sequence conflict

14

Missing in ABL14268. Ref.5

Sequence conflict

1

E → D in AAU21494. Ref.2

Sequence conflict

1

E → D in AAT00599. Ref.4

Sequence conflict

1

E → D in ACN62248. Ref.7

Sequence conflict

1

E → D in AAK96887. Ref.8

Sequences

	Sequence		Length	Mass (Da)	Tools
	Isoform 1 (P1) [UniParc]. Last modified May 15, 2007. Version 2. Checksum: B8BB91C8D8C143AB Show »	FASTA	172	20,114
	10 20 30 40 50 60 MAKLTILVAL ALFLLAAHAS ARQQWELQGD RRCQSQLERA NLRPCEQHLM QKIQRDEDSY 70 80 90 100 110 120 GRDPYSPSQD PYSPSQDPDR RDPYSPSPYD RRGAGSSQHQ ERCCNELNEF ENNQRCMCEA 130 140 150 160 170 LQQIMENQSD RLQGRQQEQQ FKRELRNLPQ QCGLRAPQRC DLEVESGGRD RY « Hide
	Isoform 2 (P2) [UniParc]. Checksum: B6C7DDB9E32E3E07 Show »	FASTA	160	18,700
	10 20 30 40 50 60 MAKLTILVAL ALFLLAAHAS ARQQWELQGD RRCQSQLERA NLRPCEQHLM QKIQRDEDSY 70 80 90 100 110 120 GRDPYSPSQD PYSPSPYDRR GAGSSQHQER CCNELNEFEN NQRCMCEALQ QIMENQSDRL 130 140 150 160 QGRQQEQQFK RELRNLPQQC GLRAPQRCDL EVESGGRDRY « Hide
	Isoform 3 (P3) [UniParc]. Checksum: 01C8D8C143AB90BD Show »	FASTA	170	19,795
	10 20 30 40 50 60 MAKLTILVAL ALFLLAAHAS ARQQWELQGD RRCQSQLERA NLRPCEQHLM QKIQRDEDSY 70 80 90 100 110 120 GRDPYSPSQD PYSPSQDPDR RDPYSPSPYD RRGAGSSQHQ ERCCNELNEF ENNQRCMCEA 130 140 150 160 170 LQQIMENQSD RLQGRQQEQQ FKRELRNLPQ QCGLRAPQRC DLEVESGGRD « Hide
	Isoform 4 (P4) [UniParc]. Checksum: EDB9E32E3E079A4B Show »	FASTA	158	18,380
	10 20 30 40 50 60 MAKLTILVAL ALFLLAAHAS ARQQWELQGD RRCQSQLERA NLRPCEQHLM QKIQRDEDSY 70 80 90 100 110 120 GRDPYSPSQD PYSPSPYDRR GAGSSQHQER CCNELNEFEN NQRCMCEALQ QIMENQSDRL 130 140 150 QGRQQEQQFK RELRNLPQQC GLRAPQRCDL EVESGGRD « Hide

References

[1]	"Isolation and characterization of two complete Ara h 2 isoforms cDNA." Chatel J.-M., Bernard H., Orson F.M. Int. Arch. Allergy Immunol. 131:14-18(2003) [PubMed: 12759484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 22-31, MASS SPECTROMETRY.
[2]	"Isolation of peanut genes encoding arachins and conglutins by expressed sequence tags." Yan Y.-S., Lin X.-D., Zhang Y.-S., Wang L., Wu K., Huang S.-Z. Plant Sci. 169:439-445(2005) [Agricola: IND43739496] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: cv. Shanyou 523. Tissue: Cotyledon.
[3]	"Chromosomal and phylogenetic context for conglutin genes in Arachis based on genomic sequence." Ramos M.L., Fleming G., Chu Y., Akiyama Y., Gallo M., Ozias-Akins P. Mol. Genet. Genomics 275:578-592(2006) [PubMed: 16614814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. F78-1339.
[4]	"cDNA cloning of peanut seed storage protein." Yan Y.-S., Wang L., Liao B., Li H., Lin X.-D., Huang S.-Z. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: cv. Shanyou 523.
[5]	"Isolation of peanut genes encoding seed storage proteins and stress proteins from developing cotyledons by expressed sequence tags." Fu G., Yan Y.-S., Wang L., Zhong Y., Huang S.-Z. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Shanyou 523.
[6]	"Cloning and characterization of four genes encoding peanut seed oleosins." Li C., Fu G., Zhong Y., Yan Y., Wang L., Huang S. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Shanyou 523.
[7]	"Proteolytical processing of Ara h 2 into mature form." Radosavljevic J., Dobrijevic D., Blanusa M., Jadranin M., Cirkovic Velickovic T. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]	"Isolation and molecular characterization of the first genomic clone of a major peanut allergen, Ara h 2." Viquez O.M., Summer C.G., Dodo H.W. J. Allergy Clin. Immunol. 107:713-717(2001) [PubMed: 11295663] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-168. Strain: cv. F78-1339. Tissue: Seed.
[9]	"Seed-specific, developmentally regulated genes of peanut." Paik-Ro O.G., Seib J.C., Smith R.L. Theor. Appl. Genet. 104:236-240(2002) [PubMed: 12582692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-168 (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. FL435. Tissue: Seed.
[10]	"Re-investigation of the major peanut allergen Arah2 on the molecular level." Becker W.-M., Suhr M., Lindner B., Wicklein D., Lepp U. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-172 (ISOFORM 1).
[11]	"Primary sequence and site-selective hydroxylation of prolines in isoforms of a major peanut allergen protein Ara h 2." Li J., Shefcheck K., Callahan J., Fenselau C. Protein Sci. 19:174-182(2010) [PubMed: 19937656] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-172 (ISOFORMS 1; 2; 3 AND 4), MASS SPECTROMETRY, HYDROXYLATION AT PRO-67; PRO-74 AND PRO-86, DISULFIDE BONDS.
[12]	"Suppression of seed storage proteins upon water stress in Arachis hypogea var. M-13 seeds." Katam R., Vasanthaiah H.K.N., Basha S.M., McClung S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 22-33; 117-131; 147-155 AND 160-169, REPRESSION BY WATER STRESS. Strain: cv. M13. Tissue: Seed.
[13]	"Structure and stability of 2S albumin-type peanut allergens: implications for the severity of peanut allergic reactions." Lehmann K., Schweimer K., Reese G., Randow S., Suhr M., Becker W.-M., Vieths S., Roesch P. Biochem. J. 395:463-472(2006) [PubMed: 16372900] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-31 AND 93-99, ALLERGEN, RESISTANCE TO HEAT AND PROTEOLYSIS.
[14]	"The major peanut allergen, Ara h 2, functions as a trypsin inhibitor, and roasting enhances this function." Maleki S.J., Viquez O.M., Jacks T., Dodo H.W., Champagne E.T., Chung S.-Y., Landry S.J. J. Allergy Clin. Immunol. 112:190-195(2003) [PubMed: 12847498] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY158467 mRNA. Translation: AAN77576.1. AY581853 mRNA. Translation: AAT00598.1. Different initiation. AY722689 mRNA. Translation: AAU21494.1. Different initiation. EF609644 Genomic DNA. Translation: ABQ96215.1. AY581854 mRNA. Translation: AAT00599.1. Different initiation. EF080817 mRNA. Translation: ABL14268.1. EF695402 Genomic DNA. Translation: ABS28872.1. FJ713110 Genomic DNA. Translation: ACN62248.1. AY007229 Genomic DNA. Translation: AAK96887.1. AF366560 mRNA. Translation: AAO61750.1. AY117434 mRNA. Translation: AAM78596.1.
3D structure databases
HSSP	HSSP built from PDB template 1W2Q based on UniProtKB Q647G9.
ProteinModelPortal	Q6PSU2.
SMR	Q6PSU2. Positions 30-169.
ModBase	Search...
Protein family/group databases
Allergome	1081. Ara h 2.0101. 1082. Ara h 2.0201. 51. Ara h 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016140. Bifunc_inhib/LTP/seed_store. IPR003612. LTP/seed_store/tryp_amyl_inhib. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Gene3D	G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CONG7_ARAHY

Accession

Primary (citable) accession number: Q6PSU2
Secondary accession number(s): A1DZE8

expand/collapse secondary AC list

Q941R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	February 8, 2011
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Nomenclature of allergens and list of entries

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents