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Protein

Atlastin-1

Gene

Atl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate Golgi biogenesis. May regulate axonal development.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 818GTPBy similarity
Nucleotide bindingi118 – 1203GTPBy similarity
Nucleotide bindingi217 – 2182GTPBy similarity
Nucleotide bindingi276 – 2794GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • identical protein binding Source: UniProtKB

GO - Biological processi

  • axonogenesis Source: UniProtKB
  • endoplasmic reticulum organization Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Atlastin-1 (EC:3.6.5.-)
Alternative name(s):
Spastic paraplegia 3A homolog
Gene namesi
Name:Atl1
Synonyms:Spg3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi1359232. Atl1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 449449CytoplasmicBy similarityAdd
BLAST
Transmembranei450 – 47021HelicalSequence analysisAdd
BLAST
Topological domaini471 – 4711LumenalSequence analysis
Transmembranei472 – 49221HelicalSequence analysisAdd
BLAST
Topological domaini493 – 55866CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • axon Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi cis cisterna Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi217 – 2171R → Q: Loss of GTPase activity. No effect on oligomerization. 1 Publication
Mutagenesisi239 – 2391R → C: Loss of GTPase activity. No effect on oligomerization. 1 Publication
Mutagenesisi258 – 2581H → R: Loss of GTPase activity. No effect on oligomerization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Atlastin-1PRO_0000384814Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineCombined sources
Modified residuei19 – 191PhosphoserineBy similarity
Modified residuei22 – 221PhosphoserineCombined sources
Modified residuei23 – 231PhosphoserineCombined sources
Modified residuei395 – 3951N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6PST4.
PRIDEiQ6PST4.

PTM databases

iPTMnetiQ6PST4.
SwissPalmiQ6PST4.

Expressioni

Tissue specificityi

Detected in brain where it is abundant in lamina V of the cerebral cortex. Also expressed within the hippocampus, mainly in pyramidal neurons in CA1 and CA3. Weakly expressed in the striatum and more robustly in amygdala and several thalamic nuclei. Also detected in several mesopontine nuclei (at protein level).1 Publication

Developmental stagei

Expression increases gradually from E18 through adulthood.1 Publication

Gene expression databases

GenevisibleiQ6PST4. RN.

Interactioni

Subunit structurei

Monomer as apoprotein and in the GDP-bound form. Homodimer in the GTP-bound form. Homooligomer. Interacts (via N-terminal region) with MAP4K4 (via CNH regulatory domain). Interacts with SPAST; interaction is direct (By similarity). Interacts with REEP5, RTN3 and RTN4 (via the transmembrane region) (PubMed:19665976). Interacts with REEP1 (By similarity). Interacts with CPT1C (By similarity). Interacts with ARL6IP1 (PubMed:24262037).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rtn4Q9JK11-16EBI-2410213,EBI-919989

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ6PST4. 1 interaction.
STRINGi10116.ENSRNOP00000041993.

Structurei

3D structure databases

ProteinModelPortaliQ6PST4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 309246GB1/RHD3-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 447447Redistribution to soluble cell fractionBy similarityAdd
BLAST
Regioni448 – 558111Sufficient for membrane associationBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili412 – 43928Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2037. Eukaryota.
ENOG410XR6Z. LUCA.
GeneTreeiENSGT00390000008959.
HOGENOMiHOG000234332.
InParanoidiQ6PST4.
KOiK17339.
OMAiCMEKGIQ.
OrthoDBiEOG7H4DTH.
PhylomeDBiQ6PST4.
TreeFamiTF105251.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030386. G_GB1_RHD3_dom.
IPR003191. Guanylate-bd_C.
IPR015894. Guanylate-bd_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02263. GBP. 1 hit.
[Graphical view]
SUPFAMiSSF48340. SSF48340. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51715. G_GB1_RHD3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PST4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSRRDRNS WGGFSEKSSD WSSEEEEPVR KAGPVQVLIV KDDHSFELDE
60 70 80 90 100
AALNRILLSE AVRDKEVVAV SVAGAFRKGK SFLMDFMLRY MYNQESVDWV
110 120 130 140 150
GDYNEPLTGF SWRGGSERET TGIQIWSEVF LINKLDGKKV AVLLMDTQGT
160 170 180 190 200
FDSQSTLRDS ATVFALSTMI SSIQVYNLSQ NVQEDDLQHL QLFTEYGRLA
210 220 230 240 250
MEETFLKPFQ SLIFLVRDWS FPYEFSYGAD GGAKFLEKRL KVSGNQHEEL
260 270 280 290 300
QNVRKHIHSC FTNISCFLLP HPGLKVATNP NFDGKLKEID DEFIKNLKIL
310 320 330 340 350
IPWLLSPESL DIKEINGNKI TCRGLLEYFK AYIKIYQGEE LPHPKSMLQA
360 370 380 390 400
TAEANNLAAV ATAKDTYNKK MEEICGGDKP FLAPNDLQTK HLQLKEDSVK
410 420 430 440 450
LFRGVKKMGG EEFSRRYLQQ LESEIDELYI QYIKHNDSKN IFHAARTPAT
460 470 480 490 500
LFVVIFITYV IAGVTGFIGL DIIASLCNMI MGLTLITLCT WAYIRYSGEY
510 520 530 540 550
RELGAVIDQV AAALWDQGST NEALYKLYSA AATHRHLYQQ AFPAPKSEPT

EQPEKKKI
Length:558
Mass (Da):63,375
Last modified:July 5, 2004 - v1
Checksum:i4FFDE569007E171F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY581896 mRNA. Translation: AAS89975.1.
BC092645 mRNA. Translation: AAH92645.1.
RefSeqiNP_001009831.1. NM_001009831.2.
UniGeneiRn.135117.

Genome annotation databases

EnsembliENSRNOT00000046085; ENSRNOP00000041993; ENSRNOG00000005063.
GeneIDi362750.
KEGGirno:362750.
UCSCiRGD:1359232. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY581896 mRNA. Translation: AAS89975.1.
BC092645 mRNA. Translation: AAH92645.1.
RefSeqiNP_001009831.1. NM_001009831.2.
UniGeneiRn.135117.

3D structure databases

ProteinModelPortaliQ6PST4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6PST4. 1 interaction.
STRINGi10116.ENSRNOP00000041993.

PTM databases

iPTMnetiQ6PST4.
SwissPalmiQ6PST4.

Proteomic databases

PaxDbiQ6PST4.
PRIDEiQ6PST4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000046085; ENSRNOP00000041993; ENSRNOG00000005063.
GeneIDi362750.
KEGGirno:362750.
UCSCiRGD:1359232. rat.

Organism-specific databases

CTDi51062.
RGDi1359232. Atl1.

Phylogenomic databases

eggNOGiKOG2037. Eukaryota.
ENOG410XR6Z. LUCA.
GeneTreeiENSGT00390000008959.
HOGENOMiHOG000234332.
InParanoidiQ6PST4.
KOiK17339.
OMAiCMEKGIQ.
OrthoDBiEOG7H4DTH.
PhylomeDBiQ6PST4.
TreeFamiTF105251.

Miscellaneous databases

PROiQ6PST4.

Gene expression databases

GenevisibleiQ6PST4. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030386. G_GB1_RHD3_dom.
IPR003191. Guanylate-bd_C.
IPR015894. Guanylate-bd_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02263. GBP. 1 hit.
[Graphical view]
SUPFAMiSSF48340. SSF48340. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51715. G_GB1_RHD3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cellular localization, oligomerization, and membrane association of the hereditary spastic paraplegia 3A (SPG3A) protein atlastin."
    Zhu P.-P., Patterson A., Lavoie B., Stadler J., Shoeb M., Patel R., Blackstone C.
    J. Biol. Chem. 278:49063-49071(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "SPG3A protein atlastin-1 is enriched in growth cones and promotes axon elongation during neuronal development."
    Zhu P.-P., Soderblom C., Tao-Cheng J.-H., Stadler J., Blackstone C.
    Hum. Mol. Genet. 15:1343-1353(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-217; ARG-239 AND HIS-258, DEVELOPMENTAL STAGE.
  4. "A class of dynamin-like GTPases involved in the generation of the tubular ER network."
    Hu J., Shibata Y., Zhu P.-P., Voss C., Rismanchi N., Prinz W.A., Rapoport T.A., Blackstone C.
    Cell 138:549-561(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH REEP5; RTN3 AND RTN4.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-22 AND SER-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Arl6IP1 has the ability to shape the mammalian ER membrane in a reticulon-like fashion."
    Yamamoto Y., Yoshida A., Miyazaki N., Iwasaki K., Sakisaka T.
    Biochem. J. 458:69-79(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARL6IP1.

Entry informationi

Entry nameiATLA1_RAT
AccessioniPrimary (citable) accession number: Q6PST4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.