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Reviewed, UniProtKB/Swiss-Prot Q6PKG0 (LARP1_HUMAN)

Last modified July 7, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    La-related protein 1
Alternative name(s):
    La ribonucleoprotein domain family member 1
Gene names
Name: LARP1
Synonyms: KIAA0731, LARP
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1096 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.4 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17

Sequence similarities

Belongs to the LARP family.

Contains 1 HTH La-type RNA-binding domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandRNA-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Molecular functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6PKG0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6PKG0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: MATQVEPLLP...RRDFVEAPPP → AADRVVCLAR...EEGVAGALLA
Note: Incomplete sequence. No experimental confirmation available.
Isoform 3 (identifier: Q6PKG0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.
     78-144: PLQLPGAEGP...VLTTVNGQSP → MLWRVLLSKR...PFPVLAPFSN

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 10961095La-related protein 1
PRO_0000207609

Regions

Domain397 – 48791HTH La-type RNA-binding
Compositional bias1044 – 10507Poly-Gly

Amino acid modifications

Modified residue21N-acetylalanine Ref.4
Modified residue751Phosphoserine Ref.15
Modified residue901Phosphoserine Ref.7 Ref.10 Ref.15 Ref.17
Modified residue1431Phosphoserine Ref.17
Modified residue1651Phosphoserine Ref.17
Modified residue3241Phosphoserine By similarity
Modified residue3271Phosphoserine By similarity
Modified residue5171Phosphoserine Ref.7 Ref.11 Ref.17
Modified residue5211Phosphoserine Ref.7 Ref.11 Ref.14 Ref.17
Modified residue5261Phosphothreonine Ref.4 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.17
Modified residue5481Phosphoserine Ref.6 Ref.16
Modified residue6221Phosphothreonine Ref.11
Modified residue6241Phosphothreonine Ref.11
Modified residue6271Phosphoserine Ref.6 Ref.7 Ref.11 Ref.17
Modified residue6311Phosphoserine Ref.7 Ref.17
Modified residue6491Phosphothreonine Ref.9 Ref.17
Modified residue7241Phosphothreonine Ref.17
Modified residue7661Phosphoserine Ref.7 Ref.15 Ref.17
Modified residue7741Phosphoserine Ref.4 Ref.6 Ref.7 Ref.13 Ref.15 Ref.17
Modified residue7851Phosphothreonine Ref.8 Ref.11
Modified residue7881Phosphothreonine Ref.8 Ref.11
Modified residue8231Phosphoserine Ref.15
Modified residue8241Phosphoserine Ref.7 Ref.15 Ref.17
Modified residue8451Phosphothreonine Ref.17
Modified residue8511Phosphoserine Ref.17
Modified residue8531Phosphoserine Ref.17
Modified residue10561Phosphoserine Ref.12
Modified residue10711Phosphothreonine Ref.7 Ref.12

Natural variations

Alternative sequence1 – 122122MATQV…EAPPP → AADRVVCLARRASPPSVHIA AAPEPGSPGRPGGGGEGGTG LEACRARGGGVLRGTGGGGR TPRRPGLQSGGPSSPPAPLV GLGFTAAASNWEGAAPRVNP DPSLQGLGPSAPEEGVAGAL LA in isoform 2.
VSP_015113
Alternative sequence1 – 7777Missing in isoform 3.
VSP_015114
Alternative sequence78 – 14467PLQLP…NGQSP → MLWRVLLSKRPPFPHPELDF QEAPIPSCPGRLPGRKNSVA LAAAPRKEPTGDREKPLPFP VLAPFSN in isoform 3.
VSP_015115

Experimental info

Sequence conflict778 – 7803RNT → TRP in AAH33856. Ref.1
Sequence conflict9791V → L Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 16, 2005. Version 2.
Checksum: CA3E9D30BBC101B7

FASTA1,096123,510
        10         20         30         40         50         60 
MATQVEPLLP GGATLLQAEE HGGLVRKKPP PAPEGKGEPG PNDVRGGEPD GSARRPRPPC 

        70         80         90        100        110        120 
AKPHKEGTGQ QERESPRPLQ LPGAEGPAIS DGEEGGGEPG AGGGAAGAAG AGRRDFVEAP 

       130        140        150        160        170        180 
PPKVNPWTKN ALPPVLTTVN GQSPPEHSAP AKVVRAAVPK QRKGSKVGDF GDAINWPTPG 

       190        200        210        220        230        240 
EIAHKSVQPQ SHKPQPTRKL PPKKDMKEQE KGEGSDSKES PKTKSDESGE EKNGDEDCQR 

       250        260        270        280        290        300 
GGQKKKGNKH KWVPLQIDMK PEVPREKLAS RPTRPPEPRH IPANRGEIKG SESATYVPVA 

       310        320        330        340        350        360 
PPTPAWQPEI KPEPAWHDQD ETSSVKSDGA GGARASFRGR GRGRGRGRGR GRGGTRTHFD 

       370        380        390        400        410        420 
YQFGYRKFDG VEGPRTPKYM NNITYYFDNV SSTELYSVDQ ELLKDYIKRQ IEYYFSVDNL 

       430        440        450        460        470        480 
ERDFFLRRKM DADGFLPITL IASFHRVQAL TTDISLIFAA LKDSKVVEIV DEKVRRREEP 

       490        500        510        520        530        540 
EKWPLPPIVD YSQTDFSQLL NCPEFVPRQH YQKETESAPG SPRAVTPVPT KTEEVSNLKT 

       550        560        570        580        590        600 
LPKGLSASLP DLDSENWIEV KKRPRPSPAR PKKSEESRFS HLTSLPQQLP SQQLMSKDQD 

       610        620        630        640        650        660 
EQEELDFLFD EEMEQMDGRK NTFTAWSDEE SDYEIDDRDV NKILIVTQTP HYMRRHPGGD 

       670        680        690        700        710        720 
RTGNHTSRAK MSAELAKVIN DGLFYYEQDL WAEKFEPEYS QIKQEVENFK KVNMISREQF 

       730        740        750        760        770        780 
DTLTPEPPVD PNQEVPPGPP RFQQVPTDAL ANKLFGAPEP STIARSLPTT VPESPNYRNT 

       790        800        810        820        830        840 
RTPRTPRTPQ LKDSSQTSRF YPVVKEGRTL DAKMPRKRKT RHSSNPPLES HVGWVMDSRE 

       850        860        870        880        890        900 
HRPRTASISS SPSEGTPTVG SYGCTPQSLP KFQHPSHELL KENGFTQHVY HKYRRRCLNE 

       910        920        930        940        950        960 
RKRLGIGQSQ EMNTLFRFWS FFLRDHFNKK MYEEFKQLAL EDAKEGYRYG LECLFRYYSY 

       970        980        990       1000       1010       1020 
GLEKKFRLDI FKDFQEETVK DYEAGQLYGL EKFWAFLKYS KAKNLDIDPK LQEYLGKFRR 

      1030       1040       1050       1060       1070       1080 
LEDFRVDPPM GEEGNHKRHS VVAGGGGGEG RKRCPSQSSS RPAAMISQPP TPPTGQPVRE 

      1090 
DAKWTSQHSN TQTLGK 

« Hide

Isoform 2.

Checksum: 685C2CC4BF401507
Show »

FASTA1,096122,940
Isoform 3.

Checksum: 64982CA22171B0E6
Show »

FASTA1,019116,465

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Cervix and Placenta.
[2]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-816 (ISOFORM 1).
Tissue: Brain.
[4]Bienvenut W.V., Calvo F., Matallanas D., Cooper W.N., Kolch W., Heiserich L., Boulahbel H., Gottlieb E.
Submitted (FEB-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-26; 115-123; 167-185; 252-265; 357-366; 410-422; 429-473; 524-539; 579-597; 643-654; 695-703; 718-778; 904-924; 937-944; 949-964 AND 1004-1017, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, PHOSPHORYLATION AT THR-526 AND SER-774, MASS SPECTROMETRY.
Tissue: Cervix carcinoma, Colon carcinoma and Mammary carcinoma.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 538-1096.
Tissue: Mammary cancer.
[6]"Large-scale characterization of HeLa cell nuclear phosphoproteins."
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-526; SER-548; SER-627 AND SER-774, MASS SPECTROMETRY.
Tissue: Epithelium.
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90; SER-517; SER-521; THR-526; SER-627; SER-631; SER-766; SER-774; SER-824 AND THR-1071, MASS SPECTROMETRY.
Tissue: Epithelium.
[8]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-526; THR-785 AND THR-788, MASS SPECTROMETRY.
Tissue: Epithelium.
[9]"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-526 AND THR-649, MASS SPECTROMETRY.
Tissue: Epithelium.
[10]"Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction."
Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R.
Mol. Cell. Proteomics 6:1952-1967(2007) [PubMed: 17693683] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90 AND THR-526, MASS SPECTROMETRY.
[11]"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry."
Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.
Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517; SER-521; THR-526; THR-622; THR-624; SER-627; THR-785 AND THR-788, MASS SPECTROMETRY.
[12]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1056 AND THR-1071, MASS SPECTROMETRY.
[13]"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.
Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-526 AND SER-774, MASS SPECTROMETRY.
[14]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-521, MASS SPECTROMETRY.
Tissue: Platelet.
[15]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75; SER-90; SER-766; SER-774; SER-823 AND SER-824, MASS SPECTROMETRY.
[16]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-548, MASS SPECTROMETRY.
[17]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90; SER-143; SER-165; SER-517; SER-521; THR-526; SER-627; SER-631; THR-649; THR-724; SER-766; SER-774; SER-824; THR-845; SER-851 AND SER-853, MASS SPECTROMETRY.
[18]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

BC001460 mRNA. Translation: AAH01460.2.
BC033856 mRNA. Translation: AAH33856.1. Different initiation.
AB018274 mRNA. Translation: BAA34451.1.
AK091465 mRNA. Translation: BAC03668.1.
AL133034 mRNA. Translation: CAB61364.1.
IPIIPI00185919.
IPI00411690.
IPI00643027.
PIRT42646.
RefSeqNP_056130.2.
NP_291029.2.
UniGeneHs.292078

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ6PKG0. 6 interactions.

PTM databases

PhosphoSiteQ6PKG0.

Proteomic databases

PRIDEQ6PKG0.

Genome annotation databases

EnsemblENSG00000155506. Homo sapiens. [Contig view]
GeneID23367.
KEGGhsa:23367.
UCSCuc003lvo.1. human.
uc003lvp.1. human.

Organism-specific databases

GeneCardsGC05P154073.
H-InvDBHIX0005343.
HGNCHGNC:29531. LARP1.
HPACAB015222.
MIM612059. gene.
PharmGKBPA142671564.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ6PKG0.
HOVERGENQ6PKG0.

Gene expression databases

ArrayExpressQ6PKG0.
BgeeQ6PKG0.
CleanExHS_LARP1.
GermOnlineENSG00000155506. Homo sapiens.

Family and domain databases

InterProIPR006607. DM15.
IPR006630. Lupus_La_RNA_bd.
IPR011991. Wing_hlx_DNA_bd.
[Graphical view]
Gene3DG3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PfamPF05383. La. 1 hit.
[Graphical view]
SMARTSM00684. DM15. 3 hits.
SM00715. LA. 1 hit.
[Graphical view]
PROSITEPS50961. HTH_LA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio45430.
PMAP-CutDBQ6PKG0.
SOURCESearch...

Entry information

Entry nameLARP1_HUMAN
AccessionPrimary (citable) accession number: Q6PKG0
Secondary accession number(s): O94836 expand/collapse secondary AC list , Q8N4M2, Q8NB73, Q9UFD7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: July 7, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents