Q6PKC3 (TXD11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thioredoxin domain-containing protein 11

Alternative name(s):
EF-hand-binding protein 1

Gene names

Name:	TXNDC11
Synonyms:	EFP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	985 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as a redox regulator involved in DUOX proteins folding. The interaction with DUOX1 and DUOX2 suggest that it belongs to a multiprotein complex constituting the thyroid H2O2 generating system. It is however not sufficient to assist DUOX1 and DUOX2 in H2O2 generation.
Subunit structure	Interacts with the cytoplasmic part of DUOX1 and DUOX2. Interacts with TPO and CYBA. Ref.5
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Tissue specificity	Widely expressed at low level. Expressed at higher level in thyroid and prostate. Ref.5
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.
Sequence caution	The sequence AAD20043.1 differs from that shown. Reason: Frameshift at position 618. The sequence AAH13727.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB55129.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC11044.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Redox-active center Repeat Transmembrane Transmembrane helix
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6PKC3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6PKC3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     265-291: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6PKC3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     265-278: ALESTSSPRALVSF → DGVSPCRPGWSAVA
     279-985: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 985	985	Thioredoxin domain-containing protein 11		PRO_0000120173
Regions
	Transmembrane	65 – 85	21	Helical; Potential
	Domain	92 – 214	123	Thioredoxin 1
	Domain	649 – 799	151	Thioredoxin 2
	Coiled coil	821 – 919	99	Potential
Amino acid modifications
	Modified residue	828	1	Phosphoserine Ref.6
	Disulfide bond	469 ↔ 472		Redox-active By similarity
	Disulfide bond	719 ↔ 722		Redox-active By similarity
Natural variations
	Alternative sequence	265 – 291	27	Missing in isoform 2.		VSP_014335
	Alternative sequence	265 – 278	14	ALEST…ALVSF → DGVSPCRPGWSAVA in isoform 3.		VSP_014336
	Alternative sequence	279 – 985	707	Missing in isoform 3.		VSP_014337
	Natural variant	783	1	V → L. Ref.2 Corresponds to variant rs3190321 [ dbSNP | Ensembl ].		VAR_022767

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2005. Version 2. Checksum: 4A8C852F8E81B8BC	FASTA	985	110,529
	10 20 30 40 50 60 MSECGGRGGG SSSSEDAEDE GGGGGGPAGS DCLSSSPTLA TASSAGRLRR GLRGAFLMAR 70 80 90 100 110 120 QRPELLCGAV ALGCALLLAL KFTCSRAKDV IIPAKPPVSF FSLRSPVLDL FQGQLDYAEY 130 140 150 160 170 180 VRRDSEVVLL FFYAPWCGQS IAARAEIEQA ASRLSDQVLF VAINCWWNQG KCRKQKHFFY 190 200 210 220 230 240 FPVIYLYHRS FGPIEYKGPM SAVYIEKFVR RVMKPLLYIP SQSELLDFLS NYEPGVLGYF 250 260 270 280 290 300 EFSGSPQPPG YLTFFTSALH SLKKALESTS SPRALVSFTG EWHLETKIYV LDYLGTVRFG 310 320 330 340 350 360 VITNKHLAKL VSLVHSGSVY LHRHFNTSLV FPREVLNYTA ENICKWALEN QETLFRWLRP 370 380 390 400 410 420 HGGKSLLLNN ELKKGPALFL FIPFNPLAES HPLIDEITEV ALEYNNCHGD QVVERLLQHL 430 440 450 460 470 480 RRVDAPVLES LALEVPAQLP DPPTITASPC CNTVVLPQWH SFSRTHNVCE LCVNQTSGGM 490 500 510 520 530 540 KPSSVSVPQC SFFEMAAALD SFYLKEQTFY HVASDSIECS NFLTSYSPFS YYTACCRTIS 550 560 570 580 590 600 RGVSGFIDSE QGVFEAPTVA FSSLEKKCEV DAPSSVPHIE ENRYLFPEVD MTSTNFTGLS 610 620 630 640 650 660 CRTNKTLNIY LLDSNLFWLY AERLGAPSST QVKEFAAIVD VKEESHYILD PKQALMKLTL 670 680 690 700 710 720 ESFIQNFSVL YSPLKRHLIG SGSAQFPSQH LITEVTTDTF WEVVLQKQDV LLLYYAPWCG 730 740 750 760 770 780 FCPSLNHIFI QLARNLPMDT FTVARIDVSQ NDLPWEFMVD RLPTVLFFPC NRKDLSVKYP 790 800 810 820 830 840 EDVPITLPNL LRFILHHSDP ASSPQNVANS PTKECLQSEA VLQRGHISHL EREIQKLRAE 850 860 870 880 890 900 ISSLQRAQVQ VESQLSSARR DEHRLRQQQR ALEEQHSLLH AHSEQLQALY EQKTRELQEL 910 920 930 940 950 960 ARKLQELADA SENLLTENTW LKILVATMER KLEGRDGAES LAAQREVHPK QPEPSATPQL 970 980 PGSSPPPANV SATLVSERNK ENRTD
	Isoform 2 [UniParc]. Checksum: 7E051E7BFA61B4F8	FASTA	958	107,512
	10 20 30 40 50 60 MSECGGRGGG SSSSEDAEDE GGGGGGPAGS DCLSSSPTLA TASSAGRLRR GLRGAFLMAR 70 80 90 100 110 120 QRPELLCGAV ALGCALLLAL KFTCSRAKDV IIPAKPPVSF FSLRSPVLDL FQGQLDYAEY 130 140 150 160 170 180 VRRDSEVVLL FFYAPWCGQS IAARAEIEQA ASRLSDQVLF VAINCWWNQG KCRKQKHFFY 190 200 210 220 230 240 FPVIYLYHRS FGPIEYKGPM SAVYIEKFVR RVMKPLLYIP SQSELLDFLS NYEPGVLGYF 250 260 270 280 290 300 EFSGSPQPPG YLTFFTSALH SLKKDYLGTV RFGVITNKHL AKLVSLVHSG SVYLHRHFNT 310 320 330 340 350 360 SLVFPREVLN YTAENICKWA LENQETLFRW LRPHGGKSLL LNNELKKGPA LFLFIPFNPL 370 380 390 400 410 420 AESHPLIDEI TEVALEYNNC HGDQVVERLL QHLRRVDAPV LESLALEVPA QLPDPPTITA 430 440 450 460 470 480 SPCCNTVVLP QWHSFSRTHN VCELCVNQTS GGMKPSSVSV PQCSFFEMAA ALDSFYLKEQ 490 500 510 520 530 540 TFYHVASDSI ECSNFLTSYS PFSYYTACCR TISRGVSGFI DSEQGVFEAP TVAFSSLEKK 550 560 570 580 590 600 CEVDAPSSVP HIEENRYLFP EVDMTSTNFT GLSCRTNKTL NIYLLDSNLF WLYAERLGAP 610 620 630 640 650 660 SSTQVKEFAA IVDVKEESHY ILDPKQALMK LTLESFIQNF SVLYSPLKRH LIGSGSAQFP 670 680 690 700 710 720 SQHLITEVTT DTFWEVVLQK QDVLLLYYAP WCGFCPSLNH IFIQLARNLP MDTFTVARID 730 740 750 760 770 780 VSQNDLPWEF MVDRLPTVLF FPCNRKDLSV KYPEDVPITL PNLLRFILHH SDPASSPQNV 790 800 810 820 830 840 ANSPTKECLQ SEAVLQRGHI SHLEREIQKL RAEISSLQRA QVQVESQLSS ARRDEHRLRQ 850 860 870 880 890 900 QQRALEEQHS LLHAHSEQLQ ALYEQKTREL QELARKLQEL ADASENLLTE NTWLKILVAT 910 920 930 940 950 MERKLEGRDG AESLAAQREV HPKQPEPSAT PQLPGSSPPP ANVSATLVSE RNKENRTD
	Isoform 3 [UniParc]. Checksum: C89C635153ADE9F9	FASTA	278	30,375
	10 20 30 40 50 60 MSECGGRGGG SSSSEDAEDE GGGGGGPAGS DCLSSSPTLA TASSAGRLRR GLRGAFLMAR 70 80 90 100 110 120 QRPELLCGAV ALGCALLLAL KFTCSRAKDV IIPAKPPVSF FSLRSPVLDL FQGQLDYAEY 130 140 150 160 170 180 VRRDSEVVLL FFYAPWCGQS IAARAEIEQA ASRLSDQVLF VAINCWWNQG KCRKQKHFFY 190 200 210 220 230 240 FPVIYLYHRS FGPIEYKGPM SAVYIEKFVR RVMKPLLYIP SQSELLDFLS NYEPGVLGYF 250 260 270 EFSGSPQPPG YLTFFTSALH SLKKDGVSPC RPGWSAVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Embryo and Teratocarcinoma.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT LEU-783. Tissue: Placenta.
[3]	Mei G., Yu W., Gibbs R.A. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 523-985. Tissue: Brain.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 656-985.
[5]	"Identification of a novel partner of duox: EFP1, a thioredoxin-related protein." Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont J.E., Miot F. J. Biol. Chem. 280:3096-3103(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH DUOX1; DUOX2; TPO AND CYBA.
[6]	"Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction." Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R. Mol. Cell. Proteomics 6:1952-1967(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-828, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK027464 mRNA. Translation: BAB55129.1. Different initiation. AK027646 mRNA. Translation: BAB55262.1. AK074534 mRNA. Translation: BAC11044.1. Sequence problems. BC002856 mRNA. Translation: AAH02856.1. BC013727 mRNA. Translation: AAH13727.1. Different initiation. BC018635 mRNA. Translation: AAH18635.1. AF131780 mRNA. Translation: AAD20043.1. Frameshift. CR457152 mRNA. Translation: CAG33433.1.
IPI	IPI00074799. IPI00384063. IPI00478749.
RefSeq	NP_056998.4. NM_015914.5.
UniGene	Hs.313847.
3D structure databases
ProteinModelPortal	Q6PKC3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6PKC3. 41 interactions.
MINT	MINT-2872884.
STRING	9606.ENSP00000283033.
PTM databases
PhosphoSite	Q6PKC3.
Polymorphism databases
DMDM	68566185.
Proteomic databases
PaxDb	Q6PKC3.
PRIDE	Q6PKC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000283033; ENSP00000283033; ENSG00000153066. ENST00000356957; ENSP00000349439; ENSG00000153066.
GeneID	51061.
KEGG	hsa:51061.
UCSC	uc002dbg.1. human. uc010buu.1. human.
Organism-specific databases
CTD	51061.
GeneCards	GC16M011772.
H-InvDB	HIX0017349.
HGNC	HGNC:28030. TXNDC11.
HPA	HPA041174. HPA041390.
neXtProt	NX_Q6PKC3.
PharmGKB	PA134915251.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0526.
HOGENOM	HOG000132983.
HOVERGEN	HBG082866.
InParanoid	Q6PKC3.
OMA	NFSVLYS.
PhylomeDB	Q6PKC3.
Gene expression databases
ArrayExpress	Q6PKC3.
Bgee	Q6PKC3.
CleanEx	HS_TXNDC11.
Genevestigator	Q6PKC3.
GermOnline	ENSG00000153066. Homo sapiens.
Family and domain databases
Gene3D	3.40.30.10. 2 hits.
InterPro	IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 2 hits.
PROSITE	PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	51061.
NextBio	53645.

Entry information

Entry name

TXD11_HUMAN

Accession

Primary (citable) accession number: Q6PKC3
Secondary accession number(s): O95887 , Q6PJA6, Q8N2Q4, Q96K45, Q96K53

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.