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Protein

Leucine-rich repeat and fibronectin type-III domain-containing protein 4

Gene

LRFN4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes neurite outgrowth in hippocampal neurons. May play a role in redistributing DLG4 to the cell periphery (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-8849932. SALM protein interactions at the synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat and fibronectin type-III domain-containing protein 4
Gene namesi
Name:LRFN4
Synonyms:SALM3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:28456. LRFN4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 518ExtracellularSequence analysisAdd BLAST502
Transmembranei519 – 539HelicalSequence analysisAdd BLAST21
Topological domaini540 – 635CytoplasmicSequence analysisAdd BLAST96

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi633 – 635Missing : Loss of DLG1-, DLG3- and DLG4-binding. 1 Publication3

Organism-specific databases

OpenTargetsiENSG00000173621.
PharmGKBiPA134922153.

Polymorphism and mutation databases

BioMutaiLRFN4.
DMDMi62286924.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000001484317 – 635Leucine-rich repeat and fibronectin type-III domain-containing protein 4Add BLAST619

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...)Sequence analysis1
Glycosylationi70N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi302 ↔ 351PROSITE-ProRule annotation
Glycosylationi324N-linked (GlcNAc...)Sequence analysis1
Glycosylationi333N-linked (GlcNAc...)Sequence analysis1
Glycosylationi376N-linked (GlcNAc...)Sequence analysis1
Glycosylationi440N-linked (GlcNAc...)Sequence analysis1
Modified residuei585PhosphoserineCombined sources1
Modified residuei626PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ6PJG9.
PaxDbiQ6PJG9.
PeptideAtlasiQ6PJG9.
PRIDEiQ6PJG9.

PTM databases

iPTMnetiQ6PJG9.
PhosphoSitePlusiQ6PJG9.

Expressioni

Gene expression databases

BgeeiENSG00000173621.
CleanExiHS_LRFN4.
ExpressionAtlasiQ6PJG9. baseline and differential.
GenevisibleiQ6PJG9. HS.

Interactioni

Subunit structurei

Can form heteromeric complexes with LRFN1, LRFN2, LRFN3 and LRFN5. Unable to form homophilic interactions across cell junctions (By similarity). Interacts with DLG1, DLG2, DLG3 and DLG4.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NOTCH2NLQ7Z3S95EBI-7910762,EBI-945833

Protein-protein interaction databases

BioGridi122470. 41 interactors.
IntActiQ6PJG9. 29 interactors.
MINTiMINT-8379462.
STRINGi9606.ENSP00000312535.

Structurei

3D structure databases

ProteinModelPortaliQ6PJG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 48LRRNTAdd BLAST32
Repeati49 – 70LRR 1Add BLAST22
Repeati73 – 94LRR 2Add BLAST22
Repeati97 – 118LRR 3Add BLAST22
Repeati121 – 142LRR 4Add BLAST22
Repeati146 – 161LRR 5Add BLAST16
Repeati170 – 191LRR 6Add BLAST22
Repeati194 – 215LRR 7Add BLAST22
Domaini234 – 280LRRCTAdd BLAST47
Domaini281 – 367Ig-likeAdd BLAST87
Domaini405 – 502Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi632 – 635PDZ-binding4

Domaini

The PDZ-binding motif is required for neurite outgrowth promotion (By similarity). This motif is also involved in DLG1-, DLG3- and DLG4-binding.By similarity

Sequence similaritiesi

Belongs to the LRFN family.Curated
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation
Contains 7 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000118831.
HOGENOMiHOG000237343.
HOVERGENiHBG052352.
InParanoidiQ6PJG9.
KOiK16357.
OMAiPHGGNGS.
OrthoDBiEOG091G04B7.
PhylomeDBiQ6PJG9.
TreeFamiTF350185.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 2 hits.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF07679. I-set. 1 hit.
PF13855. LRR_8. 2 hits.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
SM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 6 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6PJG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPLLLLLL ASGAAACPLP CVCQNLSESL STLCAHRGLL FVPPNVDRRT
60 70 80 90 100
VELRLADNFI QALGPPDFRN MTGLVDLTLS RNAITRIGAR AFGDLESLRS
110 120 130 140 150
LHLDGNRLVE LGTGSLRGPV NLQHLILSGN QLGRIAPGAF DDFLESLEDL
160 170 180 190 200
DLSYNNLRQV PWAGIGAMPA LHTLNLDHNL IDALPPGAFA QLGQLSRLDL
210 220 230 240 250
TSNRLATLAP DPLFSRGRDA EASPAPLVLS FSGNPLHCNC ELLWLRRLAR
260 270 280 290 300
PDDLETCASP PGLAGRYFWA VPEGEFSCEP PLIARHTQRL WVLEGQRATL
310 320 330 340 350
RCRALGDPAP TMHWVGPDDR LVGNSSRARA FPNGTLEIGV TGAGDAGGYT
360 370 380 390 400
CIATNPAGEA TARVELRVLA LPHGGNSSAE GGRPGPSDIA ASARTAAEGE
410 420 430 440 450
GTLESEPAVQ VTEVTATSGL VSWGPGRPAD PVWMFQIQYN SSEDETLIYR
460 470 480 490 500
IVPASSHHFL LKHLVPGADY DLCLLALSPA AGPSDLTATR LLGCAHFSTL
510 520 530 540 550
PASPLCHALQ AHVLGGTLTV AVGGVLVAAL LVFTVALLVR GRGAGNGRLP
560 570 580 590 600
LKLSHVQSQT NGGPSPTPKA HPPRSPPPRP QRSCSLDLGD AGCYGYARRL
610 620 630
GGAWARRSHS VHGGLLGAGC RGVGGSAERL EESVV
Length:635
Mass (Da):66,860
Last modified:July 5, 2004 - v1
Checksum:i13E66645A17A92EE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_024499340V → A.2 PublicationsCorresponds to variant rs3741194dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC000207 mRNA. Translation: AAH00207.2.
BC015581 mRNA. Translation: AAH15581.2.
BC027475 mRNA. Translation: AAH27475.2.
BC094813 mRNA. Translation: AAH94813.1.
AB209005 mRNA. Translation: BAD92242.1.
CCDSiCCDS8153.1.
RefSeqiNP_076941.2. NM_024036.4.
XP_005274296.1. XM_005274239.3.
UniGeneiHs.209979.
Hs.714189.

Genome annotation databases

EnsembliENST00000309602; ENSP00000312535; ENSG00000173621.
GeneIDi78999.
KEGGihsa:78999.
UCSCiuc001ojr.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC000207 mRNA. Translation: AAH00207.2.
BC015581 mRNA. Translation: AAH15581.2.
BC027475 mRNA. Translation: AAH27475.2.
BC094813 mRNA. Translation: AAH94813.1.
AB209005 mRNA. Translation: BAD92242.1.
CCDSiCCDS8153.1.
RefSeqiNP_076941.2. NM_024036.4.
XP_005274296.1. XM_005274239.3.
UniGeneiHs.209979.
Hs.714189.

3D structure databases

ProteinModelPortaliQ6PJG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122470. 41 interactors.
IntActiQ6PJG9. 29 interactors.
MINTiMINT-8379462.
STRINGi9606.ENSP00000312535.

PTM databases

iPTMnetiQ6PJG9.
PhosphoSitePlusiQ6PJG9.

Polymorphism and mutation databases

BioMutaiLRFN4.
DMDMi62286924.

Proteomic databases

MaxQBiQ6PJG9.
PaxDbiQ6PJG9.
PeptideAtlasiQ6PJG9.
PRIDEiQ6PJG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309602; ENSP00000312535; ENSG00000173621.
GeneIDi78999.
KEGGihsa:78999.
UCSCiuc001ojr.4. human.

Organism-specific databases

CTDi78999.
GeneCardsiLRFN4.
HGNCiHGNC:28456. LRFN4.
MIMi612810. gene.
neXtProtiNX_Q6PJG9.
OpenTargetsiENSG00000173621.
PharmGKBiPA134922153.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000118831.
HOGENOMiHOG000237343.
HOVERGENiHBG052352.
InParanoidiQ6PJG9.
KOiK16357.
OMAiPHGGNGS.
OrthoDBiEOG091G04B7.
PhylomeDBiQ6PJG9.
TreeFamiTF350185.

Enzyme and pathway databases

ReactomeiR-HSA-8849932. SALM protein interactions at the synapses.

Miscellaneous databases

GenomeRNAii78999.
PROiQ6PJG9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000173621.
CleanExiHS_LRFN4.
ExpressionAtlasiQ6PJG9. baseline and differential.
GenevisibleiQ6PJG9. HS.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 2 hits.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF07679. I-set. 1 hit.
PF13855. LRR_8. 2 hits.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
SM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 6 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLRFN4_HUMAN
AccessioniPrimary (citable) accession number: Q6PJG9
Secondary accession number(s): Q4VBZ3
, Q59GV4, Q8N644, Q9BWJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.