T-cell receptor-associated transmembrane adapter 1

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Q6PIZ9 (TRAT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 70. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
T-cell receptor-associated transmembrane adapter 1

Alternative name(s):
T-cell receptor-interacting molecule
Short name=TRIM
pp29/30

Gene names

Name:	TRAT1
Synonyms:	TCRIM
ORF Names:	HSPC062

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stabilizes the TCR (T-cell antigen receptor)/CD3 complex at the surface of T-cells. Ref.4
Subunit structure	Homodimer; disulfide-linked. Interacts with CD3Z. When phosphorylated, interacts with PIK3R1. Ref.1 Ref.4
Subcellular location	Cell membrane; Single-pass type III membrane protein Ref.1.
Tissue specificity	Strongly expressed in thymus, and to a lesser extent in spleen, lymph node and peripheral blood lymphocytes. Present in T-cells and NK cells, but not B-cells (at protein level). Ref.1 Ref.5
Developmental stage	Strongly expressed in fetal thymus at weeks 17-24 of gestation. Undetectable in bone marrow and fetal liver. Ref.1
Post-translational modification	Phosphorylated on tyrosines by LCK or FYN upon TCR activation. Ref.1
Sequence caution	The sequence AAF29034.1 differs from that shown. Reason: Frameshift at position 166.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	T cell receptor signaling pathway Traceable author statement. Source: Reactome cellular defense response Traceable author statement. Source: ProtInc negative regulation of receptor recycling Inferred from direct assay Ref.4. Source: MGI negative regulation of transport Inferred from direct assay Ref.4. Source: MGI positive regulation of T cell receptor signaling pathway Inferred from direct assay Ref.4. Source: MGI positive regulation of calcium-mediated signaling Inferred from direct assay Ref.4. Source: MGI
Cellular component	T cell receptor complex Inferred from direct assay Ref.4. Source: MGI integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	phosphatidylinositol-4,5-bisphosphate 3-kinase activity Traceable author statement. Source: Reactome transmembrane receptor protein tyrosine kinase adaptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 186

186

T-cell receptor-associated transmembrane adapter 1

PRO_0000083344

Regions

Topological domain

1 – 7

Extracellular Potential

Transmembrane

8 – 28

Helical; Signal-anchor for type III membrane protein; Potential

Topological domain

29 – 186

158

Cytoplasmic Potential

Region

79 – 82

Interaction with PIK3R1

Amino acid modifications

Modified residue

Phosphotyrosine Probable

Disulfide bond

Interchain Probable

Natural variations

Natural variant

137

D → G.
Corresponds to variant rs57744779 [ dbSNP | Ensembl ].

VAR_062195

Natural variant

148

S → P.
Corresponds to variant rs61585973 [ dbSNP | Ensembl ].

VAR_062196

Experimental info

Mutagenesis

Y → F: Abolishes interaction with PIK3R1. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q6PIZ9 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 236A4BEFF427EA3E
Show »

FASTA

186

21,211

        10         20         30         40         50         60 
MSGISGCPFF LWGLLALLGL ALVISLIFNI SHYVEKQRQD KMYSYSSDHT RVDEYYIEDT 

        70         80         90        100        110        120 
PIYGNLDDMI SEPMDENCYE QMKARPEKSV NKMQEATPSA QATNETQMCY ASLDHSVKGK 

       130        140        150        160        170        180 
RRKPRKQNTH FSDKDGDEQL HAIDASVSKT TLVDSFSPES QAVEENIHDD PIRLFGLIRA 


KREPIN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"T cell receptor (TCR) interacting molecule (TRIM), a novel disulfide-linked dimer associated with the TCR-CD3-zeta complex, recruits intracellular signaling proteins to the plasma membrane." Bruyns E., Marie-Cardine A., Kirchgessner H., Sagolla K., Shevchenko A., Mann M., Autschbach F., Bensussan A., Meuer S., Schraven B. J. Exp. Med. 188:561-575(1998) [PubMed: 9687533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 152-167, IDENTIFICATION BY MASS SPECTROMETRY, DIMERIZATION, INTERACTION WITH THE TCR COMPLEX AND PIK3R1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-79.
[2]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[4]	"The transmembrane adaptor protein TRIM regulates T-cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain." Kirchgessner H., Dietrich J., Scherer J., Isomaeki P., Korinek V., Hilgert I., Bruyns E., Leo A., Cope A.P., Schraven B. J. Exp. Med. 193:1269-1284(2001) [PubMed: 11390434] [Abstract] Cited for: FUNCTION, INTERACTION WITH CD3Z.
[5]	"Transmembrane adaptor molecules: a new category of lymphoid-cell markers." Tedoldi S., Paterson J.C., Hansmann M.-L., Natkunam Y., Rudiger T., Angelisova P., Du M.Q., Roberton H., Roncador G., Sanchez L., Pozzobon M., Masir N., Barry R., Pileri S., Mason D.Y., Marafioti T., Horejsi V. Blood 107:213-221(2006) [PubMed: 16160011] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF161547 mRNA. Translation: AAF29034.1. Frameshift. BC025713 mRNA. Translation: AAH25713.1.
IPI	IPI00008993.
RefSeq	NP_057472.2. NM_016388.2.
UniGene	Hs.138701.
3D structure databases
ProteinModelPortal	Q6PIZ9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6PIZ9.
PTM databases
PhosphoSite	Q6PIZ9.
Polymorphism databases
DMDM	74749223.
Proteomic databases
PRIDE	Q6PIZ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000295756; ENSP00000295756; ENSG00000163519.
GeneID	50852.
KEGG	hsa:50852.
UCSC	uc003dxi.1. human.
Organism-specific databases
CTD	50852.
GeneCards	GC03P108541.
H-InvDB	HIX0003532.
HGNC	HGNC:30698. TRAT1.
HPA	CAB026130. HPA002356.
MIM	604962. gene.
neXtProt	NX_Q6PIZ9.
PharmGKB	PA128394655.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14171.
GeneTree	ENSGT00390000004910.
HOGENOM	HBG282516.
HOVERGEN	HBG083932.
InParanoid	Q6PIZ9.
OMA	NISHYVE.
OrthoDB	EOG41ZFC3.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q6PIZ9.
Bgee	Q6PIZ9.
CleanEx	HS_TRAT1.
Genevestigator	Q6PIZ9.
GermOnline	ENSG00000163519. Homo sapiens.
Family and domain databases
InterPro	IPR020399. T-cell_rcpt-assoc_TM_adapter-1. [Graphical view]
PANTHER	PTHR15951. PTHR15951. 1 hit.
ProDom	PD306446. T-cell_rcpt-assoc_TM_adapter-1. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	53323.
SOURCE	Search...

Entry information

Entry name

TRAT1_HUMAN

Accession

Primary (citable) accession number: Q6PIZ9
Secondary accession number(s): Q9NZX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents