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Q6PIY7

- GLD2_HUMAN

UniProt

Q6PIY7 - GLD2_HUMAN

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Protein

Poly(A) RNA polymerase GLD2

Gene

PAPD4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation.

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi213 – 2131Magnesium or manganese; catalyticBy similarity
Metal bindingi215 – 2151Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB

GO - Biological processi

  1. hematopoietic progenitor cell differentiation Source: Ensembl
  2. histone mRNA catabolic process Source: UniProtKB
  3. mRNA processing Source: UniProtKB-KW
  4. RNA polyadenylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase GLD2 (EC:2.7.7.19)
Short name:
hGLD-2
Alternative name(s):
PAP-associated domain-containing protein 4
Terminal uridylyltransferase 2
Short name:
TUTase 2
Gene namesi
Name:PAPD4
Synonyms:GLD2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:26776. PAPD4.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nuclear RNA-directed RNA polymerase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134918975.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Poly(A) RNA polymerase GLD2PRO_0000341549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621Phosphoserine1 Publication
Modified residuei69 – 691Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PIY7.
PaxDbiQ6PIY7.
PRIDEiQ6PIY7.

PTM databases

PhosphoSiteiQ6PIY7.

Expressioni

Tissue specificityi

Expressed in brain. Within brain, it is expressed in cerebellum, hippocampus and medulla.1 Publication

Gene expression databases

BgeeiQ6PIY7.
CleanExiHS_PAPD4.
ExpressionAtlasiQ6PIY7. baseline and differential.
GenevestigatoriQ6PIY7.

Interactioni

Subunit structurei

Interacts with CPEB1, CPEB2, CPSF1 and PABPC1.By similarity

Protein-protein interaction databases

BioGridi127943. 3 interactions.
IntActiQ6PIY7. 3 interactions.
STRINGi9606.ENSP00000296783.

Structurei

3D structure databases

ProteinModelPortaliQ6PIY7.
SMRiQ6PIY7. Positions 162-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini386 – 44055PAP-associatedAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi76 – 9217Nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 PAP-associated domain.Curated

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
HOGENOMiHOG000088606.
HOVERGENiHBG097533.
InParanoidiQ6PIY7.
KOiK14079.
OMAiCVEFDLN.
OrthoDBiEOG7DRJ2S.
PhylomeDBiQ6PIY7.
TreeFamiTF315661.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6PIY7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFPNSILGRP PFTPNHQQHN NFFTLSPTVY SHQQLIDAQF NFQNADLSRA
60 70 80 90 100
VSLQQLTYGN VSPIQTSASP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP
110 120 130 140 150
TVVNQIVPLS GERRYSMPPL FHTHYVPDIV RCVPPFREIA FLEPREITLP
160 170 180 190 200
EAKDKLSQQI LELFETCQQQ ISDLKKKELC RTQLQREIQL LFPQSRLFLV
210 220 230 240 250
GSSLNGFGTR SSDGDLCLVV KEEPCFFQVN QKTEARHILT LVHKHFCTRL
260 270 280 290 300
SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNIVGIRN TFLLRTYAYL
310 320 330 340 350
ENRVRPLVLV IKKWASHHQI NDASRGTLSS YSLVLMVLHY LQTLPEPILP
360 370 380 390 400
SLQKIYPESF SPAIQLHLVH QAPCNVPPYL SKNESNLGDL LLGFLKYYAT
410 420 430 440 450
EFDWNSQMIS VREAKAIPRP DGIEWRNKYI CVEEPFDGTN TARAVHEKQK
460 470 480
FDMIKDQFLK SWHRLKNKRD LNSILPVRAA VLKR
Length:484
Mass (Da):56,028
Last modified:July 5, 2004 - v1
Checksum:i26167D53ABDA979D
GO
Isoform 2 (identifier: Q6PIY7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-228: Missing.

Note: No experimental confirmation available.

Show »
Length:480
Mass (Da):55,502
Checksum:i5E765A67D255F232
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91R → H in BAC04629. (PubMed:14702039)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei225 – 2284Missing in isoform 2. 1 PublicationVSP_034324

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095818 mRNA. Translation: BAC04629.1.
CH471084 Genomic DNA. Translation: EAW95837.1.
BC026061 mRNA. Translation: AAH26061.1.
BC047581 mRNA. Translation: AAH47581.1.
CCDSiCCDS4048.1. [Q6PIY7-1]
CCDS75266.1. [Q6PIY7-2]
RefSeqiNP_001107865.1. NM_001114393.1. [Q6PIY7-1]
NP_001107866.1. NM_001114394.1. [Q6PIY7-1]
NP_001284673.1. NM_001297744.1. [Q6PIY7-2]
NP_001284674.1. NM_001297745.1.
NP_776158.2. NM_173797.3. [Q6PIY7-1]
XP_006714624.1. XM_006714561.1. [Q6PIY7-1]
UniGeneiHs.418198.

Genome annotation databases

EnsembliENST00000296783; ENSP00000296783; ENSG00000164329. [Q6PIY7-1]
ENST00000423041; ENSP00000393412; ENSG00000164329. [Q6PIY7-2]
ENST00000428308; ENSP00000396861; ENSG00000164329. [Q6PIY7-1]
ENST00000453514; ENSP00000397563; ENSG00000164329. [Q6PIY7-1]
GeneIDi167153.
KEGGihsa:167153.
UCSCiuc003kga.2. human. [Q6PIY7-2]
uc003kgb.2. human. [Q6PIY7-1]

Polymorphism databases

DMDMi74737798.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095818 mRNA. Translation: BAC04629.1 .
CH471084 Genomic DNA. Translation: EAW95837.1 .
BC026061 mRNA. Translation: AAH26061.1 .
BC047581 mRNA. Translation: AAH47581.1 .
CCDSi CCDS4048.1. [Q6PIY7-1 ]
CCDS75266.1. [Q6PIY7-2 ]
RefSeqi NP_001107865.1. NM_001114393.1. [Q6PIY7-1 ]
NP_001107866.1. NM_001114394.1. [Q6PIY7-1 ]
NP_001284673.1. NM_001297744.1. [Q6PIY7-2 ]
NP_001284674.1. NM_001297745.1.
NP_776158.2. NM_173797.3. [Q6PIY7-1 ]
XP_006714624.1. XM_006714561.1. [Q6PIY7-1 ]
UniGenei Hs.418198.

3D structure databases

ProteinModelPortali Q6PIY7.
SMRi Q6PIY7. Positions 162-469.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 127943. 3 interactions.
IntActi Q6PIY7. 3 interactions.
STRINGi 9606.ENSP00000296783.

PTM databases

PhosphoSitei Q6PIY7.

Polymorphism databases

DMDMi 74737798.

Proteomic databases

MaxQBi Q6PIY7.
PaxDbi Q6PIY7.
PRIDEi Q6PIY7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000296783 ; ENSP00000296783 ; ENSG00000164329 . [Q6PIY7-1 ]
ENST00000423041 ; ENSP00000393412 ; ENSG00000164329 . [Q6PIY7-2 ]
ENST00000428308 ; ENSP00000396861 ; ENSG00000164329 . [Q6PIY7-1 ]
ENST00000453514 ; ENSP00000397563 ; ENSG00000164329 . [Q6PIY7-1 ]
GeneIDi 167153.
KEGGi hsa:167153.
UCSCi uc003kga.2. human. [Q6PIY7-2 ]
uc003kgb.2. human. [Q6PIY7-1 ]

Organism-specific databases

CTDi 167153.
GeneCardsi GC05P078908.
HGNCi HGNC:26776. PAPD4.
MIMi 614121. gene.
neXtProti NX_Q6PIY7.
PharmGKBi PA134918975.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5260.
GeneTreei ENSGT00550000074490.
HOGENOMi HOG000088606.
HOVERGENi HBG097533.
InParanoidi Q6PIY7.
KOi K14079.
OMAi CVEFDLN.
OrthoDBi EOG7DRJ2S.
PhylomeDBi Q6PIY7.
TreeFami TF315661.

Miscellaneous databases

ChiTaRSi PAPD4. human.
GenomeRNAii 167153.
NextBioi 88659.
PROi Q6PIY7.
SOURCEi Search...

Gene expression databases

Bgeei Q6PIY7.
CleanExi HS_PAPD4.
ExpressionAtlasi Q6PIY7. baseline and differential.
Genevestigatori Q6PIY7.

Family and domain databases

InterProi IPR002058. PAP_assoc.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Smooth muscle.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Pancreas.
  4. Cited for: ENZYME ACTIVITY.
  5. "Vertebrate GLD2 poly(A) polymerases in the germline and the brain."
    Rouhana L., Wang L., Buter N., Kwak J.E., Schiltz C.A., Gonzalez T., Kelley A.E., Landry C.F., Wickens M.
    RNA 11:1117-1130(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'."
    Mullen T.E., Marzluff W.F.
    Genes Dev. 22:50-65(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: ABSENCE OF FUNCTION IN HISTONE MRNA DEGRADATION ACTIVITY.
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-69, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiGLD2_HUMAN
AccessioniPrimary (citable) accession number: Q6PIY7
Secondary accession number(s): Q86WZ2, Q8N927
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3