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Q6PIY7

- GLD2_HUMAN

UniProt

Q6PIY7 - GLD2_HUMAN

Protein

Poly(A) RNA polymerase GLD2

Gene

PAPD4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation.

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi213 – 2131Magnesium or manganese; catalyticBy similarity
    Metal bindingi215 – 2151Magnesium or manganese; catalyticBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. histone mRNA catabolic process Source: UniProtKB
    2. mRNA processing Source: UniProtKB-KW
    3. RNA polyadenylation Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) RNA polymerase GLD2 (EC:2.7.7.19)
    Short name:
    hGLD-2
    Alternative name(s):
    PAP-associated domain-containing protein 4
    Terminal uridylyltransferase 2
    Short name:
    TUTase 2
    Gene namesi
    Name:PAPD4
    Synonyms:GLD2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:26776. PAPD4.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear RNA-directed RNA polymerase complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134918975.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 484484Poly(A) RNA polymerase GLD2PRO_0000341549Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei62 – 621Phosphoserine1 Publication
    Modified residuei69 – 691Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6PIY7.
    PaxDbiQ6PIY7.
    PRIDEiQ6PIY7.

    PTM databases

    PhosphoSiteiQ6PIY7.

    Expressioni

    Tissue specificityi

    Expressed in brain. Within brain, it is expressed in cerebellum, hippocampus and medulla.1 Publication

    Gene expression databases

    ArrayExpressiQ6PIY7.
    BgeeiQ6PIY7.
    CleanExiHS_PAPD4.
    GenevestigatoriQ6PIY7.

    Interactioni

    Subunit structurei

    Interacts with CPEB1, CPEB2, CPSF1 and PABPC1.By similarity

    Protein-protein interaction databases

    BioGridi127943. 2 interactions.
    IntActiQ6PIY7. 3 interactions.
    STRINGi9606.ENSP00000296783.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6PIY7.
    SMRiQ6PIY7. Positions 162-469.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini386 – 44055PAP-associatedAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi76 – 9217Nuclear localization signalSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PAP-associated domain.Curated

    Phylogenomic databases

    eggNOGiCOG5260.
    HOGENOMiHOG000088606.
    HOVERGENiHBG097533.
    InParanoidiQ6PIY7.
    KOiK14079.
    OMAiCVEFDLN.
    OrthoDBiEOG7DRJ2S.
    PhylomeDBiQ6PIY7.
    TreeFamiTF315661.

    Family and domain databases

    InterProiIPR002058. PAP_assoc.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6PIY7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFPNSILGRP PFTPNHQQHN NFFTLSPTVY SHQQLIDAQF NFQNADLSRA    50
    VSLQQLTYGN VSPIQTSASP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP 100
    TVVNQIVPLS GERRYSMPPL FHTHYVPDIV RCVPPFREIA FLEPREITLP 150
    EAKDKLSQQI LELFETCQQQ ISDLKKKELC RTQLQREIQL LFPQSRLFLV 200
    GSSLNGFGTR SSDGDLCLVV KEEPCFFQVN QKTEARHILT LVHKHFCTRL 250
    SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNIVGIRN TFLLRTYAYL 300
    ENRVRPLVLV IKKWASHHQI NDASRGTLSS YSLVLMVLHY LQTLPEPILP 350
    SLQKIYPESF SPAIQLHLVH QAPCNVPPYL SKNESNLGDL LLGFLKYYAT 400
    EFDWNSQMIS VREAKAIPRP DGIEWRNKYI CVEEPFDGTN TARAVHEKQK 450
    FDMIKDQFLK SWHRLKNKRD LNSILPVRAA VLKR 484
    Length:484
    Mass (Da):56,028
    Last modified:July 5, 2004 - v1
    Checksum:i26167D53ABDA979D
    GO
    Isoform 2 (identifier: Q6PIY7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         225-228: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:480
    Mass (Da):55,502
    Checksum:i5E765A67D255F232
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91R → H in BAC04629. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei225 – 2284Missing in isoform 2. 1 PublicationVSP_034324

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK095818 mRNA. Translation: BAC04629.1.
    CH471084 Genomic DNA. Translation: EAW95837.1.
    BC026061 mRNA. Translation: AAH26061.1.
    BC047581 mRNA. Translation: AAH47581.1.
    CCDSiCCDS4048.1. [Q6PIY7-1]
    RefSeqiNP_001107865.1. NM_001114393.1. [Q6PIY7-1]
    NP_001107866.1. NM_001114394.1. [Q6PIY7-1]
    NP_776158.2. NM_173797.3. [Q6PIY7-1]
    XP_005248508.1. XM_005248451.1. [Q6PIY7-2]
    XP_006714624.1. XM_006714561.1. [Q6PIY7-1]
    UniGeneiHs.418198.

    Genome annotation databases

    EnsembliENST00000296783; ENSP00000296783; ENSG00000164329. [Q6PIY7-1]
    ENST00000423041; ENSP00000393412; ENSG00000164329. [Q6PIY7-2]
    ENST00000428308; ENSP00000396861; ENSG00000164329. [Q6PIY7-1]
    ENST00000453514; ENSP00000397563; ENSG00000164329. [Q6PIY7-1]
    GeneIDi167153.
    KEGGihsa:167153.
    UCSCiuc003kga.2. human. [Q6PIY7-2]
    uc003kgb.2. human. [Q6PIY7-1]

    Polymorphism databases

    DMDMi74737798.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK095818 mRNA. Translation: BAC04629.1 .
    CH471084 Genomic DNA. Translation: EAW95837.1 .
    BC026061 mRNA. Translation: AAH26061.1 .
    BC047581 mRNA. Translation: AAH47581.1 .
    CCDSi CCDS4048.1. [Q6PIY7-1 ]
    RefSeqi NP_001107865.1. NM_001114393.1. [Q6PIY7-1 ]
    NP_001107866.1. NM_001114394.1. [Q6PIY7-1 ]
    NP_776158.2. NM_173797.3. [Q6PIY7-1 ]
    XP_005248508.1. XM_005248451.1. [Q6PIY7-2 ]
    XP_006714624.1. XM_006714561.1. [Q6PIY7-1 ]
    UniGenei Hs.418198.

    3D structure databases

    ProteinModelPortali Q6PIY7.
    SMRi Q6PIY7. Positions 162-469.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127943. 2 interactions.
    IntActi Q6PIY7. 3 interactions.
    STRINGi 9606.ENSP00000296783.

    PTM databases

    PhosphoSitei Q6PIY7.

    Polymorphism databases

    DMDMi 74737798.

    Proteomic databases

    MaxQBi Q6PIY7.
    PaxDbi Q6PIY7.
    PRIDEi Q6PIY7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000296783 ; ENSP00000296783 ; ENSG00000164329 . [Q6PIY7-1 ]
    ENST00000423041 ; ENSP00000393412 ; ENSG00000164329 . [Q6PIY7-2 ]
    ENST00000428308 ; ENSP00000396861 ; ENSG00000164329 . [Q6PIY7-1 ]
    ENST00000453514 ; ENSP00000397563 ; ENSG00000164329 . [Q6PIY7-1 ]
    GeneIDi 167153.
    KEGGi hsa:167153.
    UCSCi uc003kga.2. human. [Q6PIY7-2 ]
    uc003kgb.2. human. [Q6PIY7-1 ]

    Organism-specific databases

    CTDi 167153.
    GeneCardsi GC05P078944.
    HGNCi HGNC:26776. PAPD4.
    MIMi 614121. gene.
    neXtProti NX_Q6PIY7.
    PharmGKBi PA134918975.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5260.
    HOGENOMi HOG000088606.
    HOVERGENi HBG097533.
    InParanoidi Q6PIY7.
    KOi K14079.
    OMAi CVEFDLN.
    OrthoDBi EOG7DRJ2S.
    PhylomeDBi Q6PIY7.
    TreeFami TF315661.

    Miscellaneous databases

    ChiTaRSi PAPD4. human.
    GenomeRNAii 167153.
    NextBioi 88659.
    PROi Q6PIY7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6PIY7.
    Bgeei Q6PIY7.
    CleanExi HS_PAPD4.
    Genevestigatori Q6PIY7.

    Family and domain databases

    InterProi IPR002058. PAP_assoc.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Smooth muscle.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Pancreas.
    4. Cited for: ENZYME ACTIVITY.
    5. "Vertebrate GLD2 poly(A) polymerases in the germline and the brain."
      Rouhana L., Wang L., Buter N., Kwak J.E., Schiltz C.A., Gonzalez T., Kelley A.E., Landry C.F., Wickens M.
      RNA 11:1117-1130(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'."
      Mullen T.E., Marzluff W.F.
      Genes Dev. 22:50-65(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ABSENCE OF FUNCTION IN HISTONE MRNA DEGRADATION ACTIVITY.
    7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-69, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiGLD2_HUMAN
    AccessioniPrimary (citable) accession number: Q6PIY7
    Secondary accession number(s): Q86WZ2, Q8N927
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3