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Protein

Poly(A) RNA polymerase GLD2

Gene

PAPD4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation.

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi213Magnesium or manganese; catalyticBy similarity1
Metal bindingi215Magnesium or manganese; catalyticBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • polynucleotide adenylyltransferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processmRNA processing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.19 2681

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase GLD2 (EC:2.7.7.19)
Short name:
hGLD-2
Alternative name(s):
PAP-associated domain-containing protein 4
Terminal uridylyltransferase 2
Short name:
TUTase 2
Gene namesi
Name:PAPD4
Synonyms:GLD2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000164329.13
HGNCiHGNC:26776 PAPD4
MIMi614121 gene
neXtProtiNX_Q6PIY7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000164329
PharmGKBiPA134918975

Polymorphism and mutation databases

BioMutaiPAPD4
DMDMi74737798

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003415491 – 484Poly(A) RNA polymerase GLD2Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6PIY7
MaxQBiQ6PIY7
PaxDbiQ6PIY7
PeptideAtlasiQ6PIY7
PRIDEiQ6PIY7
ProteomicsDBi67187
67188 [Q6PIY7-2]

PTM databases

iPTMnetiQ6PIY7
PhosphoSitePlusiQ6PIY7

Expressioni

Tissue specificityi

Expressed in brain. Within brain, it is expressed in cerebellum, hippocampus and medulla.1 Publication

Gene expression databases

BgeeiENSG00000164329
CleanExiHS_PAPD4
ExpressionAtlasiQ6PIY7 baseline and differential
GenevisibleiQ6PIY7 HS

Organism-specific databases

HPAiHPA054468

Interactioni

Subunit structurei

Interacts with CPEB1, CPEB2, CPSF1 and PABPC1.By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi127943, 6 interactors
IntActiQ6PIY7, 7 interactors
STRINGi9606.ENSP00000296783

Structurei

3D structure databases

ProteinModelPortaliQ6PIY7
SMRiQ6PIY7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini386 – 440PAP-associatedAdd BLAST55

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 92Nuclear localization signalSequence analysisAdd BLAST17

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2277 Eukaryota
COG5260 LUCA
GeneTreeiENSGT00550000074490
HOGENOMiHOG000088606
HOVERGENiHBG097533
InParanoidiQ6PIY7
KOiK14079
OMAiWASHHEI
OrthoDBiEOG091G0BUD
PhylomeDBiQ6PIY7
TreeFamiTF315661

Family and domain databases

InterProiView protein in InterPro
IPR002058 PAP_assoc
PfamiView protein in Pfam
PF03828 PAP_assoc, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PIY7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFPNSILGRP PFTPNHQQHN NFFTLSPTVY SHQQLIDAQF NFQNADLSRA
60 70 80 90 100
VSLQQLTYGN VSPIQTSASP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP
110 120 130 140 150
TVVNQIVPLS GERRYSMPPL FHTHYVPDIV RCVPPFREIA FLEPREITLP
160 170 180 190 200
EAKDKLSQQI LELFETCQQQ ISDLKKKELC RTQLQREIQL LFPQSRLFLV
210 220 230 240 250
GSSLNGFGTR SSDGDLCLVV KEEPCFFQVN QKTEARHILT LVHKHFCTRL
260 270 280 290 300
SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNIVGIRN TFLLRTYAYL
310 320 330 340 350
ENRVRPLVLV IKKWASHHQI NDASRGTLSS YSLVLMVLHY LQTLPEPILP
360 370 380 390 400
SLQKIYPESF SPAIQLHLVH QAPCNVPPYL SKNESNLGDL LLGFLKYYAT
410 420 430 440 450
EFDWNSQMIS VREAKAIPRP DGIEWRNKYI CVEEPFDGTN TARAVHEKQK
460 470 480
FDMIKDQFLK SWHRLKNKRD LNSILPVRAA VLKR
Length:484
Mass (Da):56,028
Last modified:July 5, 2004 - v1
Checksum:i26167D53ABDA979D
GO
Isoform 2 (identifier: Q6PIY7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-228: Missing.

Note: No experimental confirmation available.
Show »
Length:480
Mass (Da):55,502
Checksum:i5E765A67D255F232
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9R → H in BAC04629 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034324225 – 228Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095818 mRNA Translation: BAC04629.1
CH471084 Genomic DNA Translation: EAW95837.1
BC026061 mRNA Translation: AAH26061.1
BC047581 mRNA Translation: AAH47581.1
CCDSiCCDS4048.1 [Q6PIY7-1]
CCDS75266.1 [Q6PIY7-2]
RefSeqiNP_001107865.1, NM_001114393.1 [Q6PIY7-1]
NP_001107866.1, NM_001114394.1 [Q6PIY7-1]
NP_001284673.1, NM_001297744.1 [Q6PIY7-2]
NP_776158.2, NM_173797.3 [Q6PIY7-1]
XP_016864642.1, XM_017009153.1
XP_016864643.1, XM_017009154.1
XP_016864644.1, XM_017009155.1 [Q6PIY7-1]
XP_016864645.1, XM_017009156.1 [Q6PIY7-1]
XP_016864646.1, XM_017009157.1 [Q6PIY7-2]
XP_016864647.1, XM_017009158.1
XP_016864648.1, XM_017009159.1 [Q6PIY7-2]
XP_016864649.1, XM_017009160.1 [Q6PIY7-2]
UniGeneiHs.418198
Hs.720183

Genome annotation databases

EnsembliENST00000296783; ENSP00000296783; ENSG00000164329 [Q6PIY7-1]
ENST00000423041; ENSP00000393412; ENSG00000164329 [Q6PIY7-2]
ENST00000428308; ENSP00000396861; ENSG00000164329 [Q6PIY7-1]
ENST00000453514; ENSP00000397563; ENSG00000164329 [Q6PIY7-1]
GeneIDi167153
KEGGihsa:167153
UCSCiuc003kga.3 human [Q6PIY7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLD2_HUMAN
AccessioniPrimary (citable) accession number: Q6PIY7
Secondary accession number(s): Q86WZ2, Q8N927
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 5, 2004
Last modified: June 20, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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