Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inactive rhomboid protein 1

Gene

Rhbdf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rhomboid protease-like protein which has no protease activity but regulates the secretion of several ligands of the epidermal growth factor receptor. Indirectly activates the epidermal growth factor receptor signaling pathway and may thereby regulate sleep, cell survival, proliferation and migration (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Protein family/group databases

MEROPSiS54.952.

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive rhomboid protein 1
Short name:
iRhom1
Alternative name(s):
Epidermal growth factor receptor-related protein
Rhomboid family member 1
Gene namesi
Name:Rhbdf1
Synonyms:Dist1, Irhom1, Kiaa4242
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:104328. Rhbdf1.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
  • Golgi apparatus membrane By similarity; Multi-pass membrane protein Sequence analysis

  • Note: Predominantly localized in the endoplasmic reticulum membrane.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 412412CytoplasmicSequence analysisAdd
BLAST
Transmembranei413 – 43321HelicalSequence analysisAdd
BLAST
Topological domaini434 – 656223LumenalSequence analysisAdd
BLAST
Transmembranei657 – 67721HelicalSequence analysisAdd
BLAST
Topological domaini678 – 69215CytoplasmicSequence analysisAdd
BLAST
Transmembranei693 – 71321HelicalSequence analysisAdd
BLAST
Topological domaini714 – 7152LumenalSequence analysis
Transmembranei716 – 73621HelicalSequence analysisAdd
BLAST
Topological domaini737 – 74711CytoplasmicSequence analysisAdd
BLAST
Transmembranei748 – 76821HelicalSequence analysisAdd
BLAST
Topological domaini769 – 7735LumenalSequence analysis
Transmembranei774 – 79421HelicalSequence analysisAdd
BLAST
Topological domaini795 – 80410CytoplasmicSequence analysis
Transmembranei805 – 82521HelicalSequence analysisAdd
BLAST
Topological domaini826 – 85631LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 856856Inactive rhomboid protein 1PRO_0000340108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei176 – 1761PhosphoserineCombined sources
Modified residuei180 – 1801PhosphothreonineCombined sources
Modified residuei183 – 1831PhosphothreonineCombined sources
Glycosylationi584 – 5841N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ6PIX5.
PaxDbiQ6PIX5.
PRIDEiQ6PIX5.

PTM databases

iPTMnetiQ6PIX5.
PhosphoSiteiQ6PIX5.

Expressioni

Tissue specificityi

Expressed in the duodenum, as well as in fetal liver and head.1 Publication

Gene expression databases

BgeeiQ6PIX5.
ExpressionAtlasiQ6PIX5. baseline and differential.
GenevisibleiQ6PIX5. MM.

Interactioni

Subunit structurei

Homodimer, or homooligomer. Interacts with TGFA and HBEGF (By similarity). Interacts with EGF; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020524.

Structurei

3D structure databases

ProteinModelPortaliQ6PIX5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2290. Eukaryota.
COG0705. LUCA.
GeneTreeiENSGT00390000012532.
HOGENOMiHOG000154118.
HOVERGENiHBG059673.
InParanoidiQ6PIX5.
OMAiHMDCAIT.
OrthoDBiEOG7288QH.
PhylomeDBiQ6PIX5.
TreeFamiTF312988.

Family and domain databases

Gene3Di1.20.1540.10. 2 hits.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
IPR022241. Rhomboid_SP.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 3 hits.
PfamiPF01694. Rhomboid. 1 hit.
PF12595. Rhomboid_SP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PIX5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEARRDSTS SLQRKKPPWL KLDIPAAVPP AAEEPSFLQP LRRQAFLRSV
60 70 80 90 100
SMPAETARVP SPHHEPRRLV LQRQTSITQT IRRGTADWFG VSKDSDSTQK
110 120 130 140 150
WQRKSIRHCS QRYGKLKPQV IRELDLPSQD NVSLTSTETP PPLYVGPCQL
160 170 180 190 200
GMQKIIDPLA RGRAFRMADD TADGLSAPHT PVTPGAASLC SFSSSRSGFN
210 220 230 240 250
RLPRRRKRES VAKMSFRAAA ALVKGRSIRD GTLRRGQRRS FTPASFLEED
260 270 280 290 300
MVDFPDELDT SFFAREGVLH EEMSTYPDEV FESPSEAALK DWEKAPDQAD
310 320 330 340 350
LTGGALDRSE LERSHLMLPL ERGWRKQKEG GPLAPQPKVR LRQEVVSAAG
360 370 380 390 400
PRRGQRIAVP VRKLFAREKR PYGLGMVGRL TNRTYRKRID SYVKRQIEDM
410 420 430 440 450
DDHRPFFTYW LTFVHSLVTI LAVCIYGIAP VGFSQHETVD SVLRKRGVYE
460 470 480 490 500
NVKYVQQENF WIGPSSEALI HLGAKFSPCM RQDPQVHSFI LAAREREKHS
510 520 530 540 550
ACCVRNDRSG CVQTSKEECS STLAVWVKWP VHPSAPDLAG NKRQFGSVCH
560 570 580 590 600
QDPRVCDEPS SEDPHEWPED ITKWPICTKS SAGNHTNHPH MDCVITGRPC
610 620 630 640 650
CIGTKGRCEI TSREYCDFMR GYFHEEATLC SQVHCMDDVC GLLPFLNPEV
660 670 680 690 700
PDQFYRLWLS LFLHAGILHC LVSVCFQMTV LRDLEKLAGW HRIAIIYLLS
710 720 730 740 750
GITGNLASAI FLPYRAEVGP AGSQFGILAC LFVELFQSWQ ILARPWRAFF
760 770 780 790 800
KLLAVVLFLF AFGLLPWIDN FAHISGFVSG LFLSFAFLPY ISFGKFDLYR
810 820 830 840 850
KRCQIIIFQV VFLGLLAGLV VLFYFYPVRC EWCEFLTCIP FTDKFCEKYE

LDAQLH
Length:856
Mass (Da):97,292
Last modified:June 10, 2008 - v2
Checksum:iBE8A3B32058793A5
GO
Isoform 2 (identifier: Q6PIX5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     266-282: EGVLHEEMSTYPDEVFE → VRRAWGVAPSPLSLDPP
     283-856: Missing.

Note: No experimental confirmation available.
Show »
Length:282
Mass (Da):31,555
Checksum:iDF73B1A9C8E5385E
GO

Sequence cautioni

The sequence AAA02574.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAL32367.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAD90543.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271A → V in AAH27346 (PubMed:15489334).Curated
Sequence conflicti41 – 411L → V in AAH27346 (PubMed:15489334).Curated
Sequence conflicti664 – 6663HAG → LAA in AAA02574 (PubMed:8318735).Curated
Sequence conflicti674 – 6741V → I in AAH27346 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei266 – 28217EGVLH…DEVFE → VRRAWGVAPSPLSLDPP in isoform 2. 1 PublicationVSP_034190Add
BLAST
Alternative sequencei283 – 856574Missing in isoform 2. 1 PublicationVSP_034191Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220566 mRNA. Translation: BAD90543.1. Different initiation.
AY016021 Genomic DNA. Translation: AAL32367.1. Sequence problems.
AL929446 Genomic DNA. Translation: CAM16542.1.
BC023469 mRNA. Translation: AAH23469.1.
BC027346 mRNA. Translation: AAH27346.1.
M99623 mRNA. Translation: AAA02574.1. Different initiation.
CCDSiCCDS24519.1. [Q6PIX5-1]
RefSeqiNP_034247.2. NM_010117.2. [Q6PIX5-1]
UniGeneiMm.11545.

Genome annotation databases

EnsembliENSMUST00000020524; ENSMUSP00000020524; ENSMUSG00000020282. [Q6PIX5-1]
GeneIDi13650.
KEGGimmu:13650.
UCSCiuc007ijb.2. mouse. [Q6PIX5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220566 mRNA. Translation: BAD90543.1. Different initiation.
AY016021 Genomic DNA. Translation: AAL32367.1. Sequence problems.
AL929446 Genomic DNA. Translation: CAM16542.1.
BC023469 mRNA. Translation: AAH23469.1.
BC027346 mRNA. Translation: AAH27346.1.
M99623 mRNA. Translation: AAA02574.1. Different initiation.
CCDSiCCDS24519.1. [Q6PIX5-1]
RefSeqiNP_034247.2. NM_010117.2. [Q6PIX5-1]
UniGeneiMm.11545.

3D structure databases

ProteinModelPortaliQ6PIX5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020524.

Protein family/group databases

MEROPSiS54.952.

PTM databases

iPTMnetiQ6PIX5.
PhosphoSiteiQ6PIX5.

Proteomic databases

MaxQBiQ6PIX5.
PaxDbiQ6PIX5.
PRIDEiQ6PIX5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020524; ENSMUSP00000020524; ENSMUSG00000020282. [Q6PIX5-1]
GeneIDi13650.
KEGGimmu:13650.
UCSCiuc007ijb.2. mouse. [Q6PIX5-1]

Organism-specific databases

CTDi64285.
MGIiMGI:104328. Rhbdf1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2290. Eukaryota.
COG0705. LUCA.
GeneTreeiENSGT00390000012532.
HOGENOMiHOG000154118.
HOVERGENiHBG059673.
InParanoidiQ6PIX5.
OMAiHMDCAIT.
OrthoDBiEOG7288QH.
PhylomeDBiQ6PIX5.
TreeFamiTF312988.

Miscellaneous databases

ChiTaRSiRhbdf1. mouse.
PROiQ6PIX5.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PIX5.
ExpressionAtlasiQ6PIX5. baseline and differential.
GenevisibleiQ6PIX5. MM.

Family and domain databases

Gene3Di1.20.1540.10. 2 hits.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
IPR022241. Rhomboid_SP.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 3 hits.
PfamiPF01694. Rhomboid. 1 hit.
PF12595. Rhomboid_SP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Thymus.
  2. "Comparative genome analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster."
    Flint J., Tufarelli C., Peden J., Clark K., Daniels R.J., Hardison R., Miller W., Philipsen S., Tan-Un K.C., McMorrow T., Frampton J., Alter B.P., Frischauf A.-M., Higgs D.R.
    Hum. Mol. Genet. 10:371-382(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  5. "Homology of a 130-kb region enclosing the alpha-globin gene cluster, the alpha-locus controlling region, and two non-globin genes in human and mouse."
    Kielman M.F., Smits R., Devi T.S., Fodde R., Bernini L.F.
    Mamm. Genome 4:314-323(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 511-670, TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Fetal liver.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176; THR-180 AND THR-183, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver and Pancreas.
  7. "Rhomboid family pseudoproteases use the ER quality control machinery to regulate intercellular signaling."
    Zettl M., Adrain C., Strisovsky K., Lastun V., Freeman M.
    Cell 145:79-91(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EGF.

Entry informationi

Entry nameiRHDF1_MOUSE
AccessioniPrimary (citable) accession number: Q6PIX5
Secondary accession number(s): A2AVE2
, Q04843, Q5DTF4, Q6PJ49, Q8VIK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: June 8, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.