Q6PIL6 (KCIP4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kv channel-interacting protein 4
Short name=KChIP4

Alternative name(s):
A-type potassium channel modulatory protein 4
Calsenilin-like protein
Potassium channel-interacting protein 4

Gene names

Name:	KCNIP4
Synonyms:	CALP, KCHIP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents By similarity. Ref.1
Subunit structure	Component of heteromultimeric potassium channels By similarity. Interacts with KCND2. Interacts with KCND3, and the C-terminus of PSEN2 and probably PSEN1. Ref.1
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Tissue specificity	Predominantly expressed in brain. Ref.1
Domain	The KIS (K-channel inactivation suppressor) domain is required for converting A-type Kv4 current to a slowly inactivating delayed rectifier potassium current By similarity.
Sequence similarities	Belongs to the recoverin family. Contains 4 EF-hand domains.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calcium Potassium
Molecular function	Ionic channel Potassium channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro potassium channel activity Inferred from electronic annotation. Source: UniProtKB-KW voltage-gated ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6PIL6-1)

Also known as: KChIP4.1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6PIL6-2)

Also known as: KChIP4.2;

The sequence of this isoform differs from the canonical sequence as follows:
     21-55: GFLYAQNSTKRSIKERLMKLLPCSAAKTSSPAIQN → D

Isoform 3 (identifier: Q6PIL6-3)

Also known as: KChIP4bs; KCHIP4.2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.

Isoform 4 (identifier: Q6PIL6-4)

Also known as: KChIP4.4;

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: MNVRRVESIS...AKTSSPAIQN → MNLEGLEMIAVLIVIVLFVKLLEQFGLIEAGLED

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Kv channel-interacting protein 4		PRO_0000073825
Regions
	Domain	61 – 117	57	EF-hand 1; degenerate
	Domain	120 – 155	36	EF-hand 2
	Domain	156 – 191	36	EF-hand 3
	Domain	204 – 239	36	EF-hand 4
	Calcium binding	133 – 144	12	1 Potential
	Calcium binding	169 – 180	12	2 By similarity
	Calcium binding	217 – 228	12	3 By similarity
	Region	2 – 44	43	KIS By similarity
	Region	237 – 250	14	Interaction with KCND2 By similarity
Natural variations
	Alternative sequence	1 – 62	62	Missing in isoform 3.		VSP_015066
	Alternative sequence	1 – 55	55	MNVRR…PAIQN → MNLEGLEMIAVLIVIVLFVK LLEQFGLIEAGLED in isoform 4.		VSP_015067
	Alternative sequence	21 – 55	35	GFLYA…PAIQN → D in isoform 2.		VSP_015068
Experimental info
	Sequence conflict	29	1	T → I in AAG36974. Ref.1
	Sequence conflict	29	1	T → I in AAK53713. Ref.3
	Sequence conflict	32	1	S → N in AAG36974. Ref.1
	Sequence conflict	32	1	S → N in AAK53713. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (KChIP4.1) [UniParc]. Last modified July 5, 2004. Version 1. Checksum: DC6C758D73468092	FASTA	250	28,729
	10 20 30 40 50 60 MNVRRVESIS AQLEEASSTG GFLYAQNSTK RSIKERLMKL LPCSAAKTSS PAIQNSVEDE 70 80 90 100 110 120 LEMATVRHRP EALELLEAQS KFTKKELQIL YRGFKNECPS GVVNEETFKE IYSQFFPQGD 130 140 150 160 170 180 STTYAHFLFN AFDTDHNGAV SFEDFIKGLS ILLRGTVQEK LNWAFNLYDI NKDGYITKEE 190 200 210 220 230 240 MLDIMKAIYD MMGKCTYPVL KEDAPRQHVE TFFQKMDKNK DGVVTIDEFI ESCQKDENIM 250 RSMQLFENVI
	Isoform 2 (KChIP4.2) [UniParc]. Checksum: 17C4AFD3B4CFF23C	FASTA	216	25,008
	10 20 30 40 50 60 MNVRRVESIS AQLEEASSTG DSVEDELEMA TVRHRPEALE LLEAQSKFTK KELQILYRGF 70 80 90 100 110 120 KNECPSGVVN EETFKEIYSQ FFPQGDSTTY AHFLFNAFDT DHNGAVSFED FIKGLSILLR 130 140 150 160 170 180 GTVQEKLNWA FNLYDINKDG YITKEEMLDI MKAIYDMMGK CTYPVLKEDA PRQHVETFFQ 190 200 210 KMDKNKDGVV TIDEFIESCQ KDENIMRSMQ LFENVI
	Isoform 3 (KChIP4bs) (KCHIP4.2) [UniParc]. Checksum: 9F46CA3A8A308410	FASTA	188	21,945
	10 20 30 40 50 60 MATVRHRPEA LELLEAQSKF TKKELQILYR GFKNECPSGV VNEETFKEIY SQFFPQGDST 70 80 90 100 110 120 TYAHFLFNAF DTDHNGAVSF EDFIKGLSIL LRGTVQEKLN WAFNLYDINK DGYITKEEML 130 140 150 160 170 180 DIMKAIYDMM GKCTYPVLKE DAPRQHVETF FQKMDKNKDG VVTIDEFIES CQKDENIMRS MQLFENVI
	Isoform 4 (KChIP4.4) [UniParc]. Checksum: 08DF864F1A838BE8	FASTA	229	26,502
	10 20 30 40 50 60 MNLEGLEMIA VLIVIVLFVK LLEQFGLIEA GLEDSVEDEL EMATVRHRPE ALELLEAQSK 70 80 90 100 110 120 FTKKELQILY RGFKNECPSG VVNEETFKEI YSQFFPQGDS TTYAHFLFNA FDTDHNGAVS 130 140 150 160 170 180 FEDFIKGLSI LLRGTVQEKL NWAFNLYDIN KDGYITKEEM LDIMKAIYDM MGKCTYPVLK 190 200 210 220 EDAPRQHVET FFQKMDKNKD GVVTIDEFIE SCQKDENIMR SMQLFENVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of CALP/KChIP4, a novel EF-hand protein interacting with presenilin 2 and voltage-gated potassium channel subunit Kv4." Morohashi Y., Hatano N., Ohya S., Takikawa R., Watabiki T., Takasugi N., Imaizumi Y., Tomita T., Iwatsubo T. J. Biol. Chem. 277:14965-14975(2002) [PubMed: 11847232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH KCND2; PSEN1 AND PSEN2.
[2]	"Elimination of fast inactivation in Kv4 A-type potassium channels by an auxiliary subunit domain." Holmqvist M.H., Cao J., Hernandez-Pineda R., Jacobson M.D., Carroll K.I., Sung M.A., Betty M., Ge P., Gilbride K.J., Brown M.E., Jurman M.E., Lawson D., Silos-Santiago I., Xie Y., Covarrubias M., Rhodes K.J., Distefano P.S., An W.F. Proc. Natl. Acad. Sci. U.S.A. 99:1035-1040(2002) [PubMed: 11805342] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	Isbrandt D., Pongs O. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF302044 mRNA. Translation: AAG36974.1. AF305072 mRNA. Translation: AAG36977.1. AF453246 mRNA. Translation: AAL86769.1. AF367023 mRNA. Translation: AAK53712.1. AF367024 mRNA. Translation: AAK53713.1. AY029176 mRNA. Translation: AAK31594.1. AY118170 mRNA. Translation: AAM81578.1. AC104065 Genomic DNA. Translation: AAY40911.1. AC109636 Genomic DNA. No translation available. BC032520 mRNA. Translation: AAH32520.1.
IPI	IPI00022716. IPI00160317. IPI00168749. IPI00419434.
RefSeq	NP_001030176.1. NM_001035004.1. NP_079497.2. NM_025221.5. NP_671710.1. NM_147181.3. NP_671711.1. NM_147182.3. NP_671712.1. NM_147183.3.
UniGene	Hs.655705.
3D structure databases
ProteinModelPortal	Q6PIL6.
SMR	Q6PIL6. Positions 53-250.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6PIL6.
PTM databases
PhosphoSite	Q6PIL6.
Polymorphism databases
DMDM	73621129.
Proteomic databases
PRIDE	Q6PIL6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000049743; ENSRNOP00000042016; ENSRNOG00000032350. ENST00000413487; ENSP00000398943; ENSG00000185774.
GeneID	80333.
KEGG	hsa:80333.
UCSC	uc003gqd.2. human. uc010iel.1. human.
Organism-specific databases
CTD	80333.
GeneCards	GC04M020814.
HGNC	HGNC:30083. KCNIP4.
HPA	HPA022862.
MIM	608182. gene.
neXtProt	NX_Q6PIL6.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00560000076973.
HOVERGEN	HBG108179.
OrthoDB	EOG4K6G52.
PhylomeDB	Q6PIL6.
Gene expression databases
ArrayExpress	Q6PIL6.
Bgee	Q6PIL6.
CleanEx	HS_KCNIP4.
Genevestigator	Q6PIL6.
GermOnline	ENSG00000185774. Homo sapiens.
Family and domain databases
InterPro	IPR018248. EF-hand. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. IPR001125. Recoverin. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 1 hit. [Graphical view]
PRINTS	PR00450. RECOVERIN.
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	70886.
SOURCE	Search...

Entry information

Entry name

KCIP4_HUMAN

Accession

Primary (citable) accession number: Q6PIL6
Secondary accession number(s): Q4W5G8 , Q8NEU0, Q9BWT2, Q9H294, Q9H2A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.