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Protein

Sodium/potassium-transporting ATPase subunit alpha-2

Gene

Atp1a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity).By similarity

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei374 – 37414-aspartylphosphate intermediateBy similarity
Metal bindingi714 – 7141MagnesiumBy similarity
Metal bindingi718 – 7181MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. chaperone binding Source: MGI
  3. metal ion binding Source: UniProtKB-KW
  4. sodium:potassium-exchanging ATPase activity Source: UniProtKB
  5. steroid hormone binding Source: MGI

GO - Biological processi

  1. adult locomotory behavior Source: MGI
  2. ATP biosynthetic process Source: InterPro
  3. ATP hydrolysis coupled proton transport Source: Ensembl
  4. cellular potassium ion homeostasis Source: MGI
  5. cellular response to mechanical stimulus Source: Ensembl
  6. cellular response to steroid hormone stimulus Source: BHF-UCL
  7. cellular sodium ion homeostasis Source: MGI
  8. locomotion Source: MGI
  9. negative regulation of cytosolic calcium ion concentration Source: MGI
  10. negative regulation of heart contraction Source: MGI
  11. negative regulation of striated muscle contraction Source: MGI
  12. neurotransmitter uptake Source: MGI
  13. potassium ion import Source: MGI
  14. regulation of blood pressure Source: MGI
  15. regulation of cardiac muscle cell contraction Source: Ensembl
  16. regulation of muscle contraction Source: MGI
  17. regulation of respiratory gaseous exchange by neurological system process Source: MGI
  18. regulation of smooth muscle contraction Source: MGI
  19. regulation of striated muscle contraction Source: MGI
  20. regulation of the force of heart contraction Source: MGI
  21. regulation of vasoconstriction Source: MGI
  22. response to glycoside Source: BHF-UCL
  23. response to nicotine Source: Ensembl
  24. sodium ion export from cell Source: MGI
  25. visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

ReactomeiREACT_250224. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-2 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-2 subunit
Alternative name(s):
Na(+)/K(+) ATPase alpha(+) subunit
Sodium pump subunit alpha-2
Gene namesi
Name:Atp1a2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:88106. Atp1a2.

Subcellular locationi

Membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini6 – 8580CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei86 – 10621HelicalSequence AnalysisAdd
BLAST
Topological domaini107 – 12923ExtracellularSequence AnalysisAdd
BLAST
Transmembranei130 – 15021HelicalSequence AnalysisAdd
BLAST
Topological domaini151 – 286136CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei287 – 30620HelicalSequence AnalysisAdd
BLAST
Topological domaini307 – 31812ExtracellularSequence AnalysisAdd
BLAST
Transmembranei319 – 33618HelicalSequence AnalysisAdd
BLAST
Topological domaini337 – 769433CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei770 – 78920HelicalSequence AnalysisAdd
BLAST
Topological domaini790 – 79910ExtracellularSequence Analysis
Transmembranei800 – 82021HelicalSequence AnalysisAdd
BLAST
Topological domaini821 – 84020CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei841 – 86323HelicalSequence AnalysisAdd
BLAST
Topological domaini864 – 91552ExtracellularSequence AnalysisAdd
BLAST
Transmembranei916 – 93520HelicalSequence AnalysisAdd
BLAST
Topological domaini936 – 94813CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei949 – 96719HelicalSequence AnalysisAdd
BLAST
Topological domaini968 – 98215ExtracellularSequence AnalysisAdd
BLAST
Transmembranei983 – 100321HelicalSequence AnalysisAdd
BLAST
Topological domaini1004 – 102017CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. caveola Source: Ensembl
  2. cytoplasm Source: UniProtKB
  3. dendritic spine Source: Ensembl
  4. endosome Source: Ensembl
  5. myelin sheath Source: UniProtKB
  6. plasma membrane Source: UniProtKB
  7. sarcolemma Source: BHF-UCL
  8. sodium:potassium-exchanging ATPase complex Source: MGI
  9. synapse Source: Ensembl
  10. T-tubule Source: BHF-UCL
  11. vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 55By similarityPRO_0000002505
Chaini6 – 10201015Sodium/potassium-transporting ATPase subunit alpha-2PRO_0000002506Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei570 – 5701PhosphothreonineBy similarity
Modified residuei587 – 5871PhosphoserineBy similarity
Modified residuei940 – 9401Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PIE5.
PaxDbiQ6PIE5.
PRIDEiQ6PIE5.

PTM databases

PhosphoSiteiQ6PIE5.

Expressioni

Gene expression databases

BgeeiQ6PIE5.
CleanExiMM_ATP1A2.
ExpressionAtlasiQ6PIE5. baseline and differential.
GenevestigatoriQ6PIE5.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.By similarity

Protein-protein interaction databases

BioGridi221101. 1 interaction.
DIPiDIP-48355N.
IntActiQ6PIE5. 3 interactions.
MINTiMINT-7543414.
STRINGi10090.ENSMUSP00000083077.

Structurei

3D structure databases

ProteinModelPortaliQ6PIE5.
SMRiQ6PIE5. Positions 28-1020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 823Interaction with phosphoinositide-3 kinaseBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00760000119003.
HOVERGENiHBG004298.
InParanoidiQ6PIE5.
KOiK01539.
OMAiFIIINIP.
OrthoDBiEOG7327N0.
PhylomeDBiQ6PIE5.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030333. ATP1A2.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF229. PTHR24093:SF229. 1 hit.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6PIE5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG
60 70 80 90 100
RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI
110 120 130 140 150
LLWIGALLCF LAYGILAAME DEPSNDNLYL GIVLAAVVIV TGCFSYYQEA
160 170 180 190 200
KSSKIMDSFK NMVPQQALVI REGEKMQINA EEVVVGDLVE VKGGDRVPAD
210 220 230 240 250
LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG
260 270 280 290 300
TARGIVIATG DRTVMGRIAT LASGLEVGQT PIAMEIEHFI QLITGVAVFL
310 320 330 340 350
GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA
360 370 380 390 400
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT
410 420 430 440 450
TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS
460 470 480 490 500
ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDSPQSH
510 520 530 540 550
VLVMKGAPER ILDRCSTILV QGKEIPLDKE MQDAFQNAYM ELGGLGERVL
560 570 580 590 600
GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV
610 620 630 640 650
GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS
660 670 680 690 700
QVNPREAKAC VVHGSDLKDM TSEQLDEILR DHTEIVFART SPQQKLIIVE
710 720 730 740 750
GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGISGSDVSK QAADMILLDD
760 770 780 790 800
NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG
810 820 830 840 850
TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNSQTDK LVNERLISMA
860 870 880 890 900
YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR TTNDLEDSYG
910 920 930 940 950
QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI
960 970 980 990 1000
LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE
1010 1020
VRKLILRRYP GGWVEKETYY
Length:1,020
Mass (Da):112,217
Last modified:July 5, 2004 - v1
Checksum:i5436E795BD5B4CFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC036127 mRNA. Translation: AAH36127.1.
BC041774 mRNA. Translation: AAH41774.1.
CCDSiCCDS35782.1.
RefSeqiNP_848492.1. NM_178405.3.
UniGeneiMm.207432.

Genome annotation databases

EnsembliENSMUST00000085913; ENSMUSP00000083077; ENSMUSG00000007097.
GeneIDi98660.
KEGGimmu:98660.
UCSCiuc007dqc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC036127 mRNA. Translation: AAH36127.1.
BC041774 mRNA. Translation: AAH41774.1.
CCDSiCCDS35782.1.
RefSeqiNP_848492.1. NM_178405.3.
UniGeneiMm.207432.

3D structure databases

ProteinModelPortaliQ6PIE5.
SMRiQ6PIE5. Positions 28-1020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221101. 1 interaction.
DIPiDIP-48355N.
IntActiQ6PIE5. 3 interactions.
MINTiMINT-7543414.
STRINGi10090.ENSMUSP00000083077.

PTM databases

PhosphoSiteiQ6PIE5.

Proteomic databases

MaxQBiQ6PIE5.
PaxDbiQ6PIE5.
PRIDEiQ6PIE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085913; ENSMUSP00000083077; ENSMUSG00000007097.
GeneIDi98660.
KEGGimmu:98660.
UCSCiuc007dqc.1. mouse.

Organism-specific databases

CTDi477.
MGIiMGI:88106. Atp1a2.

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00760000119003.
HOVERGENiHBG004298.
InParanoidiQ6PIE5.
KOiK01539.
OMAiFIIINIP.
OrthoDBiEOG7327N0.
PhylomeDBiQ6PIE5.
TreeFamiTF312838.

Enzyme and pathway databases

ReactomeiREACT_250224. Ion transport by P-type ATPases.

Miscellaneous databases

NextBioi353565.
PROiQ6PIE5.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PIE5.
CleanExiMM_ATP1A2.
ExpressionAtlasiQ6PIE5. baseline and differential.
GenevestigatoriQ6PIE5.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030333. ATP1A2.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF229. PTHR24093:SF229. 1 hit.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  2. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry nameiAT1A2_MOUSE
AccessioniPrimary (citable) accession number: Q6PIE5
Secondary accession number(s): Q80UZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: July 5, 2004
Last modified: February 4, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.