Calcium/calmodulin-dependent protein kinase type II subunit delta

Names and origin

Protein names

Recommended name:
    Calcium/calmodulin-dependent protein kinase type II subunit delta
      Short name=CaM kinase II subunit delta
      Short name=CaMK-II subunit delta
    EC=2.7.11.17

Gene names

Name:	Camk2d
Synonyms:	Kiaa4163

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Autophosphorylation of CAMK2 plays an important role in the regulation of the kinase activity By similarity.
Subunit structure	CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with RRAD By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

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Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	G1/S transition of mitotic cell cycle Inferred from mutant phenotype. Source: MGI calcium ion transport Inferred from direct assay. Source: MGI cardiac muscle contraction Inferred from direct assay. Source: MGI protein amino acid autophosphorylation Inferred from mutant phenotype. Source: MGI
Cellular component	T-tubule Inferred from direct assay. Source: MGI calcium- and calmodulin-dependent protein kinase complex Traceable author statement. Source: UniProtKB cytoplasm Inferred from direct assay. Source: MGI neuromuscular junction Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6PHZ2-1)

Also known as: Delta 2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6PHZ2-2)

Also known as: Delta 6;

The sequence of this isoform differs from the canonical sequence as follows:
478-478: K → N
479-499: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sequence conflict

478

1

N → K in BAE25062. Ref.1

Isoform 3 (identifier: Q6PHZ2-4)

Also known as: Delta 10;

The sequence of this isoform differs from the canonical sequence as follows:
     329-329: E → EPQTTVIHNPDGNKE
     478-478: K → N
     479-499: Missing.

Isoform 4 (identifier: Q6PHZ2-5)

Also known as: Delta 5;

The sequence of this isoform differs from the canonical sequence as follows:
     328-328: K → KINNKANVVTSPKENIPTPALEPQTTVIHNPDGNK
     478-478: K → N
     479-499: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed By similarity

Chain

2 – 499

498

Calcium/calmodulin-dependent protein kinase type II subunit delta

PRO_0000277817

Regions

Domain

14 – 272

259

Protein kinase

Nucleotide binding

20 – 28

9

ATP By similarity

Sites

Active site

136

1

Proton acceptor By similarity

Binding site

43

1

ATP By similarity

Amino acid modifications

Modified residue

2

1

N-acetylalanine By similarity

Modified residue

3

1

Phosphoserine By similarity

Modified residue

231

1

Phosphotyrosine Ref.5

Modified residue

287

1

Phosphothreonine Ref.4

Modified residue

306

1

Phosphothreonine By similarity

Modified residue

307

1

Phosphothreonine By similarity

Modified residue

315

1

Phosphoserine By similarity

Modified residue

319

1

Phosphoserine By similarity

Modified residue

330

1

Phosphoserine Ref.7

Modified residue

331

1

Phosphothreonine By similarity

Modified residue

333

1

Phosphoserine By similarity

Modified residue

334

1

Phosphoserine By similarity

Modified residue

336

1

Phosphothreonine By similarity

Modified residue

337

1

Phosphothreonine Ref.7 Ref.6

Modified residue

404

1

Phosphoserine By similarity

Modified residue

441

1

Phosphoserine By similarity

Modified residue

470

1

Phosphoserine By similarity

Modified residue

472

1

Phosphoserine By similarity

Modified residue

490

1

Phosphoserine By similarity

Natural variations

Alternative sequence

328

1

K → KINNKANVVTSPKENIPTPA LEPQTTVIHNPDGNK in isoform 4.

VSP_023100

Alternative sequence

329

1

E → EPQTTVIHNPDGNKE in isoform 3.

VSP_023101

Alternative sequence

478

1

K → N in isoform 2, isoform 3 and isoform 4.

VSP_023103

Alternative sequence

479 – 499

21

Missing in isoform 2, isoform 3 and isoform 4.

VSP_023102

Experimental info

Sequence conflict

123 – 125

3

SVN → AVL in BAC30232. Ref.1

Sequence conflict

129 – 132

4

LNGI → QMGV in BAC30232. Ref.1

Sequence conflict

199

1

A → V in BAC30232. Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 (Delta 2) [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 62999FBAB98120CE Show »	FASTA	499	56,369
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKES TESSNTTIED EDVKARKQEI IKVTEQLIEA 370 380 390 400 410 420 INNGDFEAYT KICDPGLTAF EPEALGNLVE GMDFHRFYFE NALSKSNKPI HTIILNPHVH 430 440 450 460 470 480 LVGDDAACIA YIRLTQYMDG SGMPKTMQSE ETRVWHRRDG KWQNVHFHRS GSPTVPIKPP 490 CIPNGKENFS GGTSLWQNI « Hide
Isoform 2 (Delta 6). Checksum: 506DA27C914E4F7E Show »	FASTA	478	54,114
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKES TESSNTTIED EDVKARKQEI IKVTEQLIEA 370 380 390 400 410 420 INNGDFEAYT KICDPGLTAF EPEALGNLVE GMDFHRFYFE NALSKSNKPI HTIILNPHVH 430 440 450 460 470 LVGDDAACIA YIRLTQYMDG SGMPKTMQSE ETRVWHRRDG KWQNVHFHRS GSPTVPIN « Hide
Isoform 3 (Delta 10). Checksum: E0FD0E736590F908 Show »	FASTA	492	55,645
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKEP QTTVIHNPDG NKESTESSNT TIEDEDVKAR 370 380 390 400 410 420 KQEIIKVTEQ LIEAINNGDF EAYTKICDPG LTAFEPEALG NLVEGMDFHR FYFENALSKS 430 440 450 460 470 480 NKPIHTIILN PHVHLVGDDA ACIAYIRLTQ YMDGSGMPKT MQSEETRVWH RRDGKWQNVH 490 FHRSGSPTVP IN « Hide
Isoform 4 (Delta 5). Checksum: 7DA5645FB35B3EAD Show »	FASTA	512	57,748
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKIN NKANVVTSPK ENIPTPALEP QTTVIHNPDG 370 380 390 400 410 420 NKESTESSNT TIEDEDVKAR KQEIIKVTEQ LIEAINNGDF EAYTKICDPG LTAFEPEALG 430 440 450 460 470 480 NLVEGMDFHR FYFENALSKS NKPIHTIILN PHVHLVGDDA ACIAYIRLTQ YMDGSGMPKT 490 500 510 MQSEETRVWH RRDGKWQNVH FHRSGSPTVP IN « Hide

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References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4). Strain: C57BL/6J. Tissue: Hypothalamus, Stomach and Sympathetic ganglion.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Fetal brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C3H/He. Tissue: Osteoblast.
[4]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-287, MASS SPECTROMETRY. Tissue: Brain.
[5]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-231, MASS SPECTROMETRY. Tissue: Brain.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-337, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330 AND THR-337, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AK039076 mRNA. Translation: BAC30232.1. AK012702 mRNA. Translation: BAB28422.1. AK142435 mRNA. Translation: BAE25062.1. AK148840 mRNA. Translation: BAE28674.1. AK220516 mRNA. Translation: BAD90304.1. Different initiation. BC052894 mRNA. Translation: AAH52894.1.
IPI	IPI00112584. IPI00406790. IPI00475044. IPI00828919.
RefSeq	NP_001020609.1. NP_001020610.1. NP_076302.1.
UniGene	Mm.255822
3D structure databases
HSSP	HSSP built from PDB template 2BDW based on UniProtKB Q9NG91.
SMR	Q6PHZ2. Positions 11-309, 336-474.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6PHZ2.
PTM databases
PhosphoSite	Q6PHZ2.
Proteomic databases
PRIDE	Q6PHZ2.
Genome annotation databases
Ensembl	ENSMUST00000066466; ENSMUSP00000063359; ENSMUSG00000053819; Mus musculus. [Genome view] ENSMUST00000106396; ENSMUSP00000102004; ENSMUSG00000053819; Mus musculus. [Genome view]
GeneID	108058.
KEGG	mmu:108058.
UCSC	uc008rgk.1. mouse.
Organism-specific databases
CTD	108058.
MGI	MGI:1341265. Camk2d.
Rouge	Search...
Phylogenomic databases
HOVERGEN	Q6PHZ2.
OrthoDB	EOG9T1M5P.
PhylomeDB	Q6PHZ2.
Enzyme and pathway databases
BRENDA	2.7.11.17. 244.
Gene expression databases
ArrayExpress	Q6PHZ2.
Bgee	Q6PHZ2.
Genevestigator	Q6PHZ2.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR015742. Ca/CaM-dep_prot_kinase_2. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit. PTHR22982:SF64. CaMKII. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	359953.
SOURCE	Search...

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Entry information

Entry name

KCC2D_MOUSE

Accession

Primary (citable) accession number: Q6PHZ2
Secondary accession number(s): Q3UF87

Q9CZE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents