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Q6PHG4

- LIAS_DANRE

UniProt

Q6PHG4 - LIAS_DANRE

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Protein

Lipoyl synthase, mitochondrial

Gene

lias

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi128 – 1281Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi133 – 1331Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi139 – 1391Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi159 – 1591Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi163 – 1631Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi166 – 1661Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:lias
ORF Names:zgc:66080
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 1

Organism-specific databases

ZFINiZDB-GENE-040426-1528. lias.

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1414MitochondrionUniRule annotationAdd
BLAST
Chaini15 – 399385Lipoyl synthase, mitochondrialPRO_0000398211Add
BLAST

Expressioni

Gene expression databases

BgeeiQ6PHG4.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000035286.

Structurei

3D structure databases

ProteinModelPortaliQ6PHG4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
GeneTreeiENSGT00390000006234.
HOGENOMiHOG000235998.
HOVERGENiHBG023328.
InParanoidiQ6PHG4.
KOiK03644.
OrthoDBiEOG7P2XS7.
PhylomeDBiQ6PHG4.
TreeFamiTF300817.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6PHG4 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALISRSCGA ASRYSSSHLF LPSKGAEAAN VYCNRLSTAA STSSSSSPSP
60 70 80 90 100
STHNDRKKDL REDGLNLQDF ISGELSEKSK WEEYRGNLKR EKGERLRLPP
110 120 130 140 150
WLKTEIPIGK NYNKLKNTLR ELNLHTVCEE ARCPNIGECW GGGEYATATA
160 170 180 190 200
TIMLMGDTCT RGCRFCSVKT ARRPPPLDPD EPYNTAKAIA AWGLDYVVLT
210 220 230 240 250
SVDRDDIPDG GAEHFAKTVS NIKERNSKIL VECLTPDFRG DLAAVEKIAL
260 270 280 290 300
SGLDVYAHNV ETVRELQRHV RDPRANFDQS LSVLRHAKKV KSSVLTKTSI
310 320 330 340 350
MLGLGETDAQ IQATLTELRD SGVDCLTLGQ YMQPTKRHLK VEEYVTPEKF
360 370 380 390
AFWEKVGQEM GFIYTASGPL VRSSYKAGEF FLKNLLEKRK TEETTATAE
Length:399
Mass (Da):44,394
Last modified:July 5, 2004 - v1
Checksum:iEAC52976CBE8D63D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 512Missing in AAI54782. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC154781 mRNA. Translation: AAI54782.1.
BC056561 mRNA. Translation: AAH56561.1.
RefSeqiNP_001103871.1. NM_001110401.1.
UniGeneiDr.16722.

Genome annotation databases

EnsembliENSDART00000036448; ENSDARP00000035286; ENSDARG00000022845.
GeneIDi393528.
KEGGidre:393528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC154781 mRNA. Translation: AAI54782.1 .
BC056561 mRNA. Translation: AAH56561.1 .
RefSeqi NP_001103871.1. NM_001110401.1.
UniGenei Dr.16722.

3D structure databases

ProteinModelPortali Q6PHG4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000035286.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000036448 ; ENSDARP00000035286 ; ENSDARG00000022845 .
GeneIDi 393528.
KEGGi dre:393528.

Organism-specific databases

CTDi 11019.
ZFINi ZDB-GENE-040426-1528. lias.

Phylogenomic databases

eggNOGi COG0320.
GeneTreei ENSGT00390000006234.
HOGENOMi HOG000235998.
HOVERGENi HBG023328.
InParanoidi Q6PHG4.
KOi K03644.
OrthoDBi EOG7P2XS7.
PhylomeDBi Q6PHG4.
TreeFami TF300817.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Miscellaneous databases

NextBioi 20814551.
PROi Q6PHG4.

Gene expression databases

Bgeei Q6PHG4.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo and Kidney.

Entry informationi

Entry nameiLIAS_DANRE
AccessioniPrimary (citable) accession number: Q6PHG4
Secondary accession number(s): A8WGN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3