Q6PHG4 (LIAS_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase, mitochondrial
EC=2.8.1.8

Alternative name(s):
Lipoate synthase
Short name=LS
Short name=Lip-syn
Lipoic acid synthase

Gene names

Name:	lias
ORF Names:	zgc:66080

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03123
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03123
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03123
Subcellular location	Mitochondrion Potential HAMAP-Rule MF_03123.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 14	14	Mitochondrion Potential
	Chain	15 – 399	385	Lipoyl synthase, mitochondrial HAMAP-Rule MF_03123		PRO_0000398211
Sites
	Metal binding	128	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	133	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	139	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	159	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	163	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	166	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Experimental info
	Sequence conflict	50 – 51	2	Missing in AAI54782. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6PHG4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EAC52976CBE8D63D	FASTA	399	44,394
	10 20 30 40 50 60 MALISRSCGA ASRYSSSHLF LPSKGAEAAN VYCNRLSTAA STSSSSSPSP STHNDRKKDL 70 80 90 100 110 120 REDGLNLQDF ISGELSEKSK WEEYRGNLKR EKGERLRLPP WLKTEIPIGK NYNKLKNTLR 130 140 150 160 170 180 ELNLHTVCEE ARCPNIGECW GGGEYATATA TIMLMGDTCT RGCRFCSVKT ARRPPPLDPD 190 200 210 220 230 240 EPYNTAKAIA AWGLDYVVLT SVDRDDIPDG GAEHFAKTVS NIKERNSKIL VECLTPDFRG 250 260 270 280 290 300 DLAAVEKIAL SGLDVYAHNV ETVRELQRHV RDPRANFDQS LSVLRHAKKV KSSVLTKTSI 310 320 330 340 350 360 MLGLGETDAQ IQATLTELRD SGVDCLTLGQ YMQPTKRHLK VEEYVTPEKF AFWEKVGQEM 370 380 390 GFIYTASGPL VRSSYKAGEF FLKNLLEKRK TEETTATAE

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo and Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC154781 mRNA. Translation: AAI54782.1. BC056561 mRNA. Translation: AAH56561.1.
IPI	IPI00492038.
RefSeq	NP_001103871.1. NM_001110401.1.
UniGene	Dr.16722.
3D structure databases
ProteinModelPortal	Q6PHG4.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000035286.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000036448; ENSDARP00000035286; ENSDARG00000022845.
GeneID	393528.
KEGG	dre:393528.
Organism-specific databases
CTD	11019.
ZFIN	ZDB-GENE-040426-1528. lias.
Phylogenomic databases
eggNOG	COG0320.
GeneTree	ENSGT00390000006234.
HOGENOM	HOG000235998.
HOVERGEN	HBG023328.
InParanoid	Q6PHG4.
KO	K03644.
OrthoDB	EOG4T1HMX.
Enzyme and pathway databases
UniPathway	UPA00538; UER00593.
Gene expression databases
Bgee	Q6PHG4.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...
Other
NextBio	20814551.

Entry information

Entry name

LIAS_DANRE

Accession

Primary (citable) accession number: Q6PHG4
Secondary accession number(s): A8WGN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program