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Q6PHG4

- LIAS_DANRE

UniProt

Q6PHG4 - LIAS_DANRE

Protein

Lipoyl synthase, mitochondrial

Gene

lias

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi128 – 1281Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi133 – 1331Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi139 – 1391Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi159 – 1591Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi163 – 1631Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi166 – 1661Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:lias
    ORF Names:zgc:66080
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 1

    Organism-specific databases

    ZFINiZDB-GENE-040426-1528. lias.

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1414MitochondrionUniRule annotationAdd
    BLAST
    Chaini15 – 399385Lipoyl synthase, mitochondrialPRO_0000398211Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiQ6PHG4.

    Interactioni

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000035286.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6PHG4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    GeneTreeiENSGT00390000006234.
    HOGENOMiHOG000235998.
    HOVERGENiHBG023328.
    InParanoidiQ6PHG4.
    KOiK03644.
    OrthoDBiEOG7P2XS7.
    PhylomeDBiQ6PHG4.
    TreeFamiTF300817.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6PHG4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALISRSCGA ASRYSSSHLF LPSKGAEAAN VYCNRLSTAA STSSSSSPSP    50
    STHNDRKKDL REDGLNLQDF ISGELSEKSK WEEYRGNLKR EKGERLRLPP 100
    WLKTEIPIGK NYNKLKNTLR ELNLHTVCEE ARCPNIGECW GGGEYATATA 150
    TIMLMGDTCT RGCRFCSVKT ARRPPPLDPD EPYNTAKAIA AWGLDYVVLT 200
    SVDRDDIPDG GAEHFAKTVS NIKERNSKIL VECLTPDFRG DLAAVEKIAL 250
    SGLDVYAHNV ETVRELQRHV RDPRANFDQS LSVLRHAKKV KSSVLTKTSI 300
    MLGLGETDAQ IQATLTELRD SGVDCLTLGQ YMQPTKRHLK VEEYVTPEKF 350
    AFWEKVGQEM GFIYTASGPL VRSSYKAGEF FLKNLLEKRK TEETTATAE 399
    Length:399
    Mass (Da):44,394
    Last modified:July 5, 2004 - v1
    Checksum:iEAC52976CBE8D63D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti50 – 512Missing in AAI54782. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC154781 mRNA. Translation: AAI54782.1.
    BC056561 mRNA. Translation: AAH56561.1.
    RefSeqiNP_001103871.1. NM_001110401.1.
    UniGeneiDr.16722.

    Genome annotation databases

    EnsembliENSDART00000036448; ENSDARP00000035286; ENSDARG00000022845.
    GeneIDi393528.
    KEGGidre:393528.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC154781 mRNA. Translation: AAI54782.1 .
    BC056561 mRNA. Translation: AAH56561.1 .
    RefSeqi NP_001103871.1. NM_001110401.1.
    UniGenei Dr.16722.

    3D structure databases

    ProteinModelPortali Q6PHG4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7955.ENSDARP00000035286.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000036448 ; ENSDARP00000035286 ; ENSDARG00000022845 .
    GeneIDi 393528.
    KEGGi dre:393528.

    Organism-specific databases

    CTDi 11019.
    ZFINi ZDB-GENE-040426-1528. lias.

    Phylogenomic databases

    eggNOGi COG0320.
    GeneTreei ENSGT00390000006234.
    HOGENOMi HOG000235998.
    HOVERGENi HBG023328.
    InParanoidi Q6PHG4.
    KOi K03644.
    OrthoDBi EOG7P2XS7.
    PhylomeDBi Q6PHG4.
    TreeFami TF300817.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Miscellaneous databases

    NextBioi 20814551.
    PROi Q6PHG4.

    Gene expression databases

    Bgeei Q6PHG4.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo and Kidney.

    Entry informationi

    Entry nameiLIAS_DANRE
    AccessioniPrimary (citable) accession number: Q6PHG4
    Secondary accession number(s): A8WGN4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3