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Protein

Glycine amidinotransferase, mitochondrial

Gene

gatm

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development (By similarity).By similarity

Catalytic activityi

L-arginine + glycine = L-ornithine + guanidinoacetate.By similarity

Pathwayi: creatine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes creatine from L-arginine and glycine.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycine amidinotransferase, mitochondrial (gatm)
  2. Guanidinoacetate N-methyltransferase (gamt)
This subpathway is part of the pathway creatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes creatine from L-arginine and glycine, the pathway creatine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei253 – 2531By similarity
Active sitei302 – 3021By similarity
Active sitei406 – 4061Amidino-cysteine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

UniPathwayiUPA00104; UER00579.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine amidinotransferase, mitochondrialBy similarity (EC:2.1.4.1)
Alternative name(s):
L-arginine:glycine amidinotransferaseBy similarity
TransamidinaseBy similarity
Gene namesi
Name:gatmImported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-021015-1. gatm.

Subcellular locationi

  • Mitochondrion inner membrane By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 422Glycine amidinotransferase, mitochondrialBy similarityPRO_0000399096
Transit peptidei1 – ?MitochondrionBy similarity

Proteomic databases

PaxDbiQ6PH19.
PRIDEiQ6PH19.

Expressioni

Tissue specificityi

Strongly expressed in neurons and glia of the brain, the lamina propria, submucosa and serosa of the small intestine, in oocytes and on the fringes of the pancreas. Not expressed in the retina, eye lens, heart or bulbus arteriosus. Expressed in the yolk syncytial layer in gastrula stage embryos, in the yolk syncytial layer and mature somites in early segmentation embryos and in the yolk syncytial layer and the liver of long-pec stage (48 h post-fertilization) embryos.2 Publications

Developmental stagei

Widely expressed during maturation of oocytes. Also expressed in embryos from gastrulation onwards in the yolk syncytial layer and somites. Expression declines in the somites, but is up-regulated in the yolk syncytial layer throughout embryonic development.2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000052640.

Structurei

3D structure databases

ProteinModelPortaliQ6PH19.
SMRiQ6PH19. Positions 63-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidinotransferase family.Sequence analysis

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IFBR. Eukaryota.
ENOG410Y45M. LUCA.
HOGENOMiHOG000231593.
HOVERGENiHBG002492.
InParanoidiQ6PH19.
KOiK00613.
PhylomeDBiQ6PH19.

Family and domain databases

InterProiIPR033195. AmidinoTrfase.
[Graphical view]
PANTHERiPTHR10488. PTHR10488. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6PH19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVRCLRGG SRGAEAAHLI GALVGRALSG RLSRASRSSS SSAAAQLPLS
60 70 80 90 100
AHEQVPEPTA EECPVCAHNE WDPLEEVIVG RAENACVPPF TVEVKANTYE
110 120 130 140 150
KYWPFYQQYG GQTFPKEHVQ KAVAEIEEMC NILQHEGVTV RRPEPVDWSL
160 170 180 190 200
EYRTPDFSST GMYAAMPRDI LMVVGNEIIE APMAWRARFF EYRAYRPLIK
210 220 230 240 250
EYFRRGARWT TAPKPTMADQ LYDQDYPIRT VEDRHKLAAQ GKFVTTEFEP
260 270 280 290 300
CFDAADFIRA GTDIFVQRSQ VTNYMGIEWM RRHLSPTYKI HIISFKDPNP
310 320 330 340 350
MHIDATFNII GPGLVLSNPD RPCRQIEMFK KAGWTVVTPP TPLIPDNHPL
360 370 380 390 400
WMSSKWLSMN VLMLDEKRVM VDANESTIQK MFESLGIKTV KVSIRHANSL
410 420
GGGFHCWTTD VRRRGTLQSY FL
Length:422
Mass (Da):48,075
Last modified:July 5, 2004 - v1
Checksum:iB453639A69E24E12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056747 mRNA. Translation: AAH56747.1.
RefSeqiNP_955825.1. NM_199531.1.
UniGeneiDr.75520.

Genome annotation databases

GeneIDi266799.
KEGGidre:266799.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056747 mRNA. Translation: AAH56747.1.
RefSeqiNP_955825.1. NM_199531.1.
UniGeneiDr.75520.

3D structure databases

ProteinModelPortaliQ6PH19.
SMRiQ6PH19. Positions 63-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000052640.

Proteomic databases

PaxDbiQ6PH19.
PRIDEiQ6PH19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi266799.
KEGGidre:266799.

Organism-specific databases

CTDi2628.
ZFINiZDB-GENE-021015-1. gatm.

Phylogenomic databases

eggNOGiENOG410IFBR. Eukaryota.
ENOG410Y45M. LUCA.
HOGENOMiHOG000231593.
HOVERGENiHBG002492.
InParanoidiQ6PH19.
KOiK00613.
PhylomeDBiQ6PH19.

Enzyme and pathway databases

UniPathwayiUPA00104; UER00579.

Miscellaneous databases

NextBioi20804835.
PROiQ6PH19.

Family and domain databases

InterProiIPR033195. AmidinoTrfase.
[Graphical view]
PANTHERiPTHR10488. PTHR10488. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: EmbryoImported.
  2. "Spatiotemporal expression of the creatine metabolism related genes agat, gamt and ct1 during zebrafish embryogenesis."
    Wang L., Zhang Y., Shao M., Zhang H.
    Int. J. Dev. Biol. 51:247-253(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  3. "Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio."
    Wang L., Chen D., Yang L., Huang S., Zhang Y., Zhang H.
    J. Fish Biol. 76:1212-1219(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiGATM_DANRE
AccessioniPrimary (citable) accession number: Q6PH19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.