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Protein

Alpha-tubulin N-acetyltransferase 1

Gene

atat1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. May be involved in neuron development. Acetylates alpha-tubulin in neurons, but not in cilia.UniRule annotation1 Publication

Catalytic activityi

Acetyl-CoA + [alpha-tubulin]-L-lysine = CoA + [alpha-tubulin]-N6-acetyl-L-lysine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei53Crucial for catalytic activityUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

  • alpha-tubulin acetylation Source: ZFIN
  • regulation of microtubule cytoskeleton organization Source: GO_Central

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-DRE-5617833 Cilium Assembly

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-tubulin N-acetyltransferase 1UniRule annotation (EC:2.3.1.108UniRule annotation)
Short name:
Alpha-TATUniRule annotation
Short name:
Alpha-TAT1UniRule annotation
Short name:
TATUniRule annotation
Alternative name(s):
Acetyltransferase mec-17 homologUniRule annotation
Gene namesi
Name:atat1UniRule annotation
Synonyms:mec17
ORF Names:si:ch211-152p11.5, zgc:65893
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 19

Organism-specific databases

ZFINiZDB-GENE-040426-2120 atat1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Coated pit, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Morpholino knockdown of the protein causes developmental defects at 48 hours post-fertilization (hpf), including cilia curved body shape, short body axis, hydrocephalus, small head and small eyes. Morphants often do not respond, or have slow startle response, when probed with a needle, consistent with neuromuscular defects.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45L → A: Reduces activity to 30%. 1 Publication1
Mutagenesisi53Q → A: Reduces activity to 1.5%. 1 Publication1
Mutagenesisi117D → A: Reduces activity to 3%. Causes the formation of a constitutive dimer in solution. 1 Publication1
Mutagenesisi126R → E: Reduces activity to 19%. 1 Publication1
Mutagenesisi131S → L: No effect. 1 Publication1
Mutagenesisi151D → A: Reduces activity to 1%. 1 Publication1
Mutagenesisi154S → A: Reduces activity to 8%. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004020671 – 297Alpha-tubulin N-acetyltransferase 1Add BLAST297

Proteomic databases

PaxDbiQ6PH17

Expressioni

Gene expression databases

BgeeiENSDARG00000004472

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

STRINGi7955.ENSDARP00000049367

Structurei

Secondary structure

1297
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 4Combined sources4
Helixi7 – 10Combined sources4
Beta strandi13 – 20Combined sources8
Helixi21 – 28Combined sources8
Helixi35 – 52Combined sources18
Helixi62 – 67Combined sources6
Beta strandi71 – 77Combined sources7
Helixi80 – 83Combined sources4
Beta strandi86 – 95Combined sources10
Beta strandi98 – 101Combined sources4
Beta strandi107 – 110Combined sources4
Beta strandi113 – 120Combined sources8
Helixi122 – 124Combined sources3
Helixi129 – 141Combined sources13
Helixi145 – 147Combined sources3
Beta strandi148 – 152Combined sources5
Helixi155 – 165Combined sources11
Beta strandi174 – 179Combined sources6
Helixi181 – 183Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4H6UX-ray2.45A/B1-188[»]
4H6ZX-ray2.70A/B1-186[»]
4HKFX-ray1.70A1-188[»]
4YRHX-ray2.86A/B2-185[»]
SMRiQ6PH17
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 184N-acetyltransferaseUniRule annotationAdd BLAST184

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 131Acetyl-CoA bindingUniRule annotation2 PublicationsAdd BLAST14
Regioni154 – 163Acetyl-CoA bindingUniRule annotation2 Publications10

Sequence similaritiesi

Belongs to the acetyltransferase ATAT1 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG4601 Eukaryota
ENOG4111Q8H LUCA
GeneTreeiENSGT00390000008276
HOGENOMiHOG000257795
HOVERGENiHBG055797
InParanoidiQ6PH17
KOiK19573
OMAiHESRQRC
OrthoDBiEOG091G0F8N
PhylomeDBiQ6PH17
TreeFamiTF315643

Family and domain databases

HAMAPiMF_03130 mec17, 1 hit
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR038746 Atat
IPR007965 GNAT_ATAT
PANTHERiPTHR12327 PTHR12327, 1 hit
PfamiView protein in Pfam
PF05301 Acetyltransf_16, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51730 GNAT_ATAT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PH17-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFPYDLNAL FPERISVLDS NLSAGRKAHG RPDPLPQVTT VIDELGKASS
60 70 80 90 100
KAQQLPAPIT SAAKLQANRH HLYLLKDGEQ NGGRGVIVGF LKVGYKKLFL
110 120 130 140 150
LDQRGAHLET EPLCVLDFYV TETLQRHGYG SELFDFMLKH KQVEPAQMAY
160 170 180 190 200
DRPSPKFLSF LEKRYDLRNS VPQVNNFVVF AGFFQSRSAV QLRKVPPRKP
210 220 230 240 250
EGEIKPYSLM EREVVREEQR VLPWPFVRPG GPPHSPPLLP SSPQSRSLSV
260 270 280 290
GSSPSRAPLR PAAATVLQQG QTPSSPLNDS CRAKRTSSLN RSRLSFH
Length:297
Mass (Da):33,202
Last modified:July 5, 2004 - v1
Checksum:i3F08861B19D635EB
GO
Isoform 2 (identifier: Q6PH17-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     189-297: AVQLRKVPPR...SLNRSRLSFH → GTPPSPLTDQ...LQGKTHQFSK

Show »
Length:305
Mass (Da):33,198
Checksum:iE6E63F28DE12E566
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040229189 – 297AVQLR…RLSFH → GTPPSPLTDQGMYGFFGPTE KSSPKKARGRDQTLFANGKR SGSGGAEGSPLAICSPRGSP PLSPSTSVISSIPFSQCGIL PQPGPASPRRGHGPPAGSDP LIPAQRQLQGKTHQFSK in isoform 2. 1 PublicationAdd BLAST109

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX511233 Genomic DNA Translation: CAM56330.1
BC056749 mRNA Translation: AAH56749.1
BC065981 mRNA Translation: AAH65981.1
RefSeqiNP_001315192.1, NM_001328263.1 [Q6PH17-1]
NP_001315193.1, NM_001328264.1
NP_998423.1, NM_213258.2 [Q6PH17-2]
UniGeneiDr.6427

Genome annotation databases

EnsembliENSDART00000049368; ENSDARP00000049367; ENSDARG00000004472 [Q6PH17-2]
ENSDART00000103922; ENSDARP00000094698; ENSDARG00000004472 [Q6PH17-1]
GeneIDi406389
KEGGidre:406389

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiATAT_DANRE
AccessioniPrimary (citable) accession number: Q6PH17
Secondary accession number(s): Q6NZT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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