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Protein

Alpha-tubulin N-acetyltransferase 1

Gene

atat1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. May be involved in neuron development. Acetylates alpha-tubulin in neurons, but not in cilia.UniRule annotation1 Publication

Catalytic activityi

Acetyl-CoA + [alpha-tubulin]-L-lysine = CoA + [alpha-tubulin]-N(6)-acetyl-L-lysine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei53Crucial for catalytic activityUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-tubulin N-acetyltransferase 1UniRule annotation (EC:2.3.1.108UniRule annotation)
Short name:
Alpha-TATUniRule annotation
Short name:
Alpha-TAT1UniRule annotation
Short name:
TATUniRule annotation
Alternative name(s):
Acetyltransferase mec-17 homologUniRule annotation
Gene namesi
Name:atat1UniRule annotation
Synonyms:mec17
ORF Names:si:ch211-152p11.5, zgc:65893
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 19

Organism-specific databases

ZFINiZDB-GENE-040426-2120. atat1.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Membraneclathrin-coated pit UniRule annotation
  • Cell junctionfocal adhesion UniRule annotation
  • Cell projectionaxon UniRule annotation
  • Cytoplasmcytoskeleton UniRule annotation
  • Cytoplasmcytoskeletonspindle UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Coated pit, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Morpholino knockdown of the protein causes developmental defects at 48 hours post-fertilization (hpf), including cilia curved body shape, short body axis, hydrocephalus, small head and small eyes. Morphants often do not respond, or have slow startle response, when probed with a needle, consistent with neuromuscular defects.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45L → A: Reduces activity to 30%. 1 Publication1
Mutagenesisi53Q → A: Reduces activity to 1.5%. 1 Publication1
Mutagenesisi117D → A: Reduces activity to 3%. Causes the formation of a constitutive dimer in solution. 1 Publication1
Mutagenesisi126R → E: Reduces activity to 19%. 1 Publication1
Mutagenesisi131S → L: No effect. 1 Publication1
Mutagenesisi151D → A: Reduces activity to 1%. 1 Publication1
Mutagenesisi154S → A: Reduces activity to 8%. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004020671 – 297Alpha-tubulin N-acetyltransferase 1Add BLAST297

Proteomic databases

PaxDbiQ6PH17.

Expressioni

Gene expression databases

BgeeiENSDARG00000004472.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

STRINGi7955.ENSDARP00000049367.

Structurei

Secondary structure

1297
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 4Combined sources4
Helixi7 – 10Combined sources4
Beta strandi13 – 20Combined sources8
Helixi21 – 28Combined sources8
Helixi35 – 52Combined sources18
Helixi62 – 67Combined sources6
Beta strandi71 – 77Combined sources7
Helixi80 – 83Combined sources4
Beta strandi86 – 95Combined sources10
Beta strandi98 – 101Combined sources4
Beta strandi107 – 110Combined sources4
Beta strandi113 – 120Combined sources8
Helixi122 – 124Combined sources3
Helixi129 – 141Combined sources13
Helixi145 – 147Combined sources3
Beta strandi148 – 152Combined sources5
Helixi155 – 165Combined sources11
Beta strandi174 – 179Combined sources6
Helixi181 – 183Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4H6UX-ray2.45A/B1-188[»]
4H6ZX-ray2.70A/B1-186[»]
4HKFX-ray1.70A1-188[»]
4YRHX-ray2.86A/B2-185[»]
SMRiQ6PH17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 184N-acetyltransferaseUniRule annotationAdd BLAST184

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 131Acetyl-CoA bindingUniRule annotation2 PublicationsAdd BLAST14
Regioni154 – 163Acetyl-CoA bindingUniRule annotation2 Publications10

Sequence similaritiesi

Belongs to the acetyltransferase ATAT1 family.UniRule annotation
Contains 1 N-acetyltransferase domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG4601. Eukaryota.
ENOG4111Q8H. LUCA.
GeneTreeiENSGT00390000008276.
HOGENOMiHOG000257795.
HOVERGENiHBG055797.
InParanoidiQ6PH17.
KOiK19573.
OMAiTEPLCVL.
OrthoDBiEOG091G0F8N.
PhylomeDBiQ6PH17.
TreeFamiTF315643.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
HAMAPiMF_03130. mec17. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR007965. GNAT_ATAT.
[Graphical view]
PfamiPF05301. Acetyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51730. GNAT_ATAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PH17-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFPYDLNAL FPERISVLDS NLSAGRKAHG RPDPLPQVTT VIDELGKASS
60 70 80 90 100
KAQQLPAPIT SAAKLQANRH HLYLLKDGEQ NGGRGVIVGF LKVGYKKLFL
110 120 130 140 150
LDQRGAHLET EPLCVLDFYV TETLQRHGYG SELFDFMLKH KQVEPAQMAY
160 170 180 190 200
DRPSPKFLSF LEKRYDLRNS VPQVNNFVVF AGFFQSRSAV QLRKVPPRKP
210 220 230 240 250
EGEIKPYSLM EREVVREEQR VLPWPFVRPG GPPHSPPLLP SSPQSRSLSV
260 270 280 290
GSSPSRAPLR PAAATVLQQG QTPSSPLNDS CRAKRTSSLN RSRLSFH
Length:297
Mass (Da):33,202
Last modified:July 5, 2004 - v1
Checksum:i3F08861B19D635EB
GO
Isoform 2 (identifier: Q6PH17-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     189-297: AVQLRKVPPR...SLNRSRLSFH → GTPPSPLTDQ...LQGKTHQFSK

Show »
Length:305
Mass (Da):33,198
Checksum:iE6E63F28DE12E566
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040229189 – 297AVQLR…RLSFH → GTPPSPLTDQGMYGFFGPTE KSSPKKARGRDQTLFANGKR SGSGGAEGSPLAICSPRGSP PLSPSTSVISSIPFSQCGIL PQPGPASPRRGHGPPAGSDP LIPAQRQLQGKTHQFSK in isoform 2. 1 PublicationAdd BLAST109

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX511233 Genomic DNA. Translation: CAM56330.1.
BC056749 mRNA. Translation: AAH56749.1.
BC065981 mRNA. Translation: AAH65981.1.
RefSeqiNP_001315192.1. NM_001328263.1. [Q6PH17-1]
NP_001315193.1. NM_001328264.1.
NP_998423.1. NM_213258.2. [Q6PH17-2]
UniGeneiDr.6427.

Genome annotation databases

EnsembliENSDART00000049368; ENSDARP00000049367; ENSDARG00000004472. [Q6PH17-2]
ENSDART00000103922; ENSDARP00000094698; ENSDARG00000004472. [Q6PH17-1]
GeneIDi406389.
KEGGidre:406389.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX511233 Genomic DNA. Translation: CAM56330.1.
BC056749 mRNA. Translation: AAH56749.1.
BC065981 mRNA. Translation: AAH65981.1.
RefSeqiNP_001315192.1. NM_001328263.1. [Q6PH17-1]
NP_001315193.1. NM_001328264.1.
NP_998423.1. NM_213258.2. [Q6PH17-2]
UniGeneiDr.6427.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4H6UX-ray2.45A/B1-188[»]
4H6ZX-ray2.70A/B1-186[»]
4HKFX-ray1.70A1-188[»]
4YRHX-ray2.86A/B2-185[»]
SMRiQ6PH17.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000049367.

Proteomic databases

PaxDbiQ6PH17.

Protocols and materials databases

DNASUi406389.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000049368; ENSDARP00000049367; ENSDARG00000004472. [Q6PH17-2]
ENSDART00000103922; ENSDARP00000094698; ENSDARG00000004472. [Q6PH17-1]
GeneIDi406389.
KEGGidre:406389.

Organism-specific databases

CTDi79969.
ZFINiZDB-GENE-040426-2120. atat1.

Phylogenomic databases

eggNOGiKOG4601. Eukaryota.
ENOG4111Q8H. LUCA.
GeneTreeiENSGT00390000008276.
HOGENOMiHOG000257795.
HOVERGENiHBG055797.
InParanoidiQ6PH17.
KOiK19573.
OMAiTEPLCVL.
OrthoDBiEOG091G0F8N.
PhylomeDBiQ6PH17.
TreeFamiTF315643.

Miscellaneous databases

PROiQ6PH17.

Gene expression databases

BgeeiENSDARG00000004472.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
HAMAPiMF_03130. mec17. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR007965. GNAT_ATAT.
[Graphical view]
PfamiPF05301. Acetyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51730. GNAT_ATAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATAT_DANRE
AccessioniPrimary (citable) accession number: Q6PH17
Secondary accession number(s): Q6NZT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.