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Q6PH17

- ATAT_DANRE

UniProt

Q6PH17 - ATAT_DANRE

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Protein
Alpha-tubulin N-acetyltransferase 1
Gene
atat1, mec17, si:ch211-152p11.5, zgc:65893
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May promote directional cell locomotion and chemotaxis By similarity. May be required for normal sperm flagellar function By similarity. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Acetylates alpha-tubulin in neurons, but not in cilia.1 Publication

Catalytic activityi

Acetyl-CoA + [alpha-tubulin]-L-lysine = CoA + [alpha-tubulin]-N(6)-acetyl-L-lysine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei53 – 531Crucial for catalytic activity

GO - Molecular functioni

  1. tubulin N-acetyltransferase activity Source: UniProtKB

GO - Biological processi

  1. alpha-tubulin acetylation Source: ZFIN
  2. neuron development Source: UniProtKB-HAMAP
  3. regulation of microtubule cytoskeleton organization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-tubulin N-acetyltransferase 1 (EC:2.3.1.108)
Short name:
Alpha-TAT
Short name:
Alpha-TAT1
Short name:
TAT
Alternative name(s):
Acetyltransferase mec-17 homolog
Gene namesi
Name:atat1
Synonyms:mec17
ORF Names:si:ch211-152p11.5, zgc:65893
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 19

Organism-specific databases

ZFINiZDB-GENE-040426-2120. atat1.

Subcellular locationi

Cytoplasm By similarity. Membraneclathrin-coated pit By similarity. Cell junctionfocal adhesion By similarity. Cell projectionaxon By similarity. Cytoplasmcytoskeleton By similarity. Cytoplasmcytoskeletonspindle By similarity UniRule annotation

GO - Cellular componenti

  1. axon Source: UniProtKB-SubCell
  2. coated pit Source: UniProtKB-SubCell
  3. cytoplasm Source: UniProtKB-SubCell
  4. focal adhesion Source: UniProtKB-SubCell
  5. spindle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Coated pit, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Morpholino knockdown of the protein causes developmental defects at 48 hours post-fertilization (hpf), including cilia curved body shape, short body axis, hydrocephalus, small head and small eyes. Morphants often do not respond, or have slow startle response, when probed with a needle, consistent with neuromuscular defects.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi45 – 451L → A: Reduces activity to 30%. 1 Publication
Mutagenesisi53 – 531Q → A: Reduces activity to 1.5%. 1 Publication
Mutagenesisi117 – 1171D → A: Reduces activity to 3%. Causes the formation of a constitutive dimer in solution. 1 Publication
Mutagenesisi126 – 1261R → E: Reduces activity to 19%. 1 Publication
Mutagenesisi131 – 1311S → L: No effect. 1 Publication
Mutagenesisi151 – 1511D → A: Reduces activity to 1%. 1 Publication
Mutagenesisi154 – 1541S → A: Reduces activity to 8%. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Alpha-tubulin N-acetyltransferase 1UniRule annotation
PRO_0000402067Add
BLAST

Expressioni

Gene expression databases

BgeeiQ6PH17.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi7955.ENSDARP00000049367.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 44
Helixi7 – 104
Beta strandi13 – 208
Helixi21 – 288
Helixi35 – 5218
Helixi62 – 676
Beta strandi71 – 777
Helixi80 – 834
Beta strandi86 – 9510
Beta strandi98 – 1014
Beta strandi107 – 1104
Beta strandi113 – 1208
Helixi122 – 1243
Helixi129 – 14113
Helixi145 – 1473
Beta strandi148 – 1525
Helixi155 – 16511
Beta strandi174 – 1796
Helixi181 – 1833

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H6UX-ray2.45A/B1-188[»]
4H6ZX-ray2.70A/B1-186[»]
4HKFX-ray1.70A1-188[»]

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 13114Acetyl-CoA bindingUniRule annotation
Add
BLAST
Regioni154 – 16310Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG249793.
GeneTreeiENSGT00390000008276.
HOGENOMiHOG000257795.
HOVERGENiHBG055797.
InParanoidiQ6NZT0.
OMAiKEENIHP.
OrthoDBiEOG76739W.
PhylomeDBiQ6PH17.
TreeFamiTF315643.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
HAMAPiMF_03130. mec17.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR007965. Alpha-TAT.
[Graphical view]
PANTHERiPTHR12327. PTHR12327. 1 hit.
PfamiPF05301. Mec-17. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6PH17-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDFPYDLNAL FPERISVLDS NLSAGRKAHG RPDPLPQVTT VIDELGKASS    50
KAQQLPAPIT SAAKLQANRH HLYLLKDGEQ NGGRGVIVGF LKVGYKKLFL 100
LDQRGAHLET EPLCVLDFYV TETLQRHGYG SELFDFMLKH KQVEPAQMAY 150
DRPSPKFLSF LEKRYDLRNS VPQVNNFVVF AGFFQSRSAV QLRKVPPRKP 200
EGEIKPYSLM EREVVREEQR VLPWPFVRPG GPPHSPPLLP SSPQSRSLSV 250
GSSPSRAPLR PAAATVLQQG QTPSSPLNDS CRAKRTSSLN RSRLSFH 297
Length:297
Mass (Da):33,202
Last modified:July 5, 2004 - v1
Checksum:i3F08861B19D635EB
GO
Isoform 2 (identifier: Q6PH17-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     189-297: AVQLRKVPPR...SLNRSRLSFH → GTPPSPLTDQ...LQGKTHQFSK

Show »
Length:305
Mass (Da):33,198
Checksum:iE6E63F28DE12E566
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei189 – 297109AVQLR…RLSFH → GTPPSPLTDQGMYGFFGPTE KSSPKKARGRDQTLFANGKR SGSGGAEGSPLAICSPRGSP PLSPSTSVISSIPFSQCGIL PQPGPASPRRGHGPPAGSDP LIPAQRQLQGKTHQFSK in isoform 2.
VSP_040229Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX511233 Genomic DNA. Translation: CAM56330.1.
BC056749 mRNA. Translation: AAH56749.1.
BC065981 mRNA. Translation: AAH65981.1.
RefSeqiNP_998423.1. NM_213258.1. [Q6PH17-2]
XP_005170079.1. XM_005170022.1. [Q6PH17-1]
UniGeneiDr.6427.

Genome annotation databases

EnsembliENSDART00000049368; ENSDARP00000049367; ENSDARG00000004472. [Q6PH17-2]
ENSDART00000103922; ENSDARP00000094698; ENSDARG00000004472. [Q6PH17-1]
GeneIDi406389.
KEGGidre:406389.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX511233 Genomic DNA. Translation: CAM56330.1 .
BC056749 mRNA. Translation: AAH56749.1 .
BC065981 mRNA. Translation: AAH65981.1 .
RefSeqi NP_998423.1. NM_213258.1. [Q6PH17-2 ]
XP_005170079.1. XM_005170022.1. [Q6PH17-1 ]
UniGenei Dr.6427.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4H6U X-ray 2.45 A/B 1-188 [» ]
4H6Z X-ray 2.70 A/B 1-186 [» ]
4HKF X-ray 1.70 A 1-188 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000049367.

Protocols and materials databases

DNASUi 406389.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000049368 ; ENSDARP00000049367 ; ENSDARG00000004472 . [Q6PH17-2 ]
ENSDART00000103922 ; ENSDARP00000094698 ; ENSDARG00000004472 . [Q6PH17-1 ]
GeneIDi 406389.
KEGGi dre:406389.

Organism-specific databases

CTDi 79969.
ZFINi ZDB-GENE-040426-2120. atat1.

Phylogenomic databases

eggNOGi NOG249793.
GeneTreei ENSGT00390000008276.
HOGENOMi HOG000257795.
HOVERGENi HBG055797.
InParanoidi Q6NZT0.
OMAi KEENIHP.
OrthoDBi EOG76739W.
PhylomeDBi Q6PH17.
TreeFami TF315643.

Miscellaneous databases

NextBioi 20817998.

Gene expression databases

Bgeei Q6PH17.

Family and domain databases

Gene3Di 3.40.630.30. 1 hit.
HAMAPi MF_03130. mec17.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR007965. Alpha-TAT.
[Graphical view ]
PANTHERi PTHR12327. PTHR12327. 1 hit.
Pfami PF05301. Mec-17. 1 hit.
[Graphical view ]
SUPFAMi SSF55729. SSF55729. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Embryo.
  3. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. "Molecular basis of the acetyltransferase activity of MEC-17 towards alpha-tubulin."
    Li W., Zhong C., Li L., Sun B., Wang W., Xu S., Zhang T., Wang C., Bao L., Ding J.
    Cell Res. 22:1707-1711(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 1-188 IN COMPLEX WITH ACETYL-COA, MUTAGENESIS OF GLN-53.
  5. "Crystal structures of tubulin acetyltransferase reveal a conserved catalytic core and the plasticity of the essential N terminus."
    Kormendi V., Szyk A., Piszczek G., Roll-Mecak A.
    J. Biol. Chem. 287:41569-41575(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 1-188 OF WILD-TYPE AND OF MUTANT ALA-117 IN COMPLEX WITH ACETYL-COA, SUBUNIT, MUTAGENESIS OF LEU-45; ASP-117; ARG-126; SER-131; ASP-151 AND SER-154.

Entry informationi

Entry nameiATAT_DANRE
AccessioniPrimary (citable) accession number: Q6PH17
Secondary accession number(s): Q6NZT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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