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Q6PGL7

- FAM21_MOUSE

UniProt

Q6PGL7 - FAM21_MOUSE

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Protein

WASH complex subunit FAM21

Gene

Fam21

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes (By similarity).By similarity

GO - Biological processi

  1. retrograde transport, endosome to Golgi Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
WASH complex subunit FAM21
Gene namesi
Name:Fam21
Synonyms:D6Wsu116e, Kiaa0592
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:106463. Fam21.

Subcellular locationi

GO - Cellular componenti

  1. early endosome Source: UniProtKB
  2. membrane Source: UniProtKB-KW
  3. WASH complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13341334WASH complex subunit FAM21PRO_0000317433Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571Phosphoserine1 Publication
Modified residuei159 – 1591Phosphoserine1 Publication
Modified residuei284 – 2841Phosphoserine1 Publication
Modified residuei388 – 3881Phosphoserine1 Publication
Modified residuei533 – 5331Phosphoserine1 Publication
Modified residuei613 – 6131Phosphoserine1 Publication
Modified residuei614 – 6141Phosphoserine2 Publications
Modified residuei723 – 7231Phosphoserine1 Publication
Modified residuei747 – 7471Phosphoserine3 Publications
Modified residuei1169 – 11691Phosphoserine1 Publication
Modified residuei1172 – 11721Phosphoserine1 Publication
Modified residuei1173 – 11731Phosphoserine1 Publication
Modified residuei1333 – 13331Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PGL7.
PaxDbiQ6PGL7.
PRIDEiQ6PGL7.

PTM databases

PhosphoSiteiQ6PGL7.

Expressioni

Gene expression databases

BgeeiQ6PGL7.
ExpressionAtlasiQ6PGL7. baseline and differential.
GenevestigatoriQ6PGL7.

Interactioni

Subunit structurei

Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH1, FAM21, KIAA1033, KIAA0196 and CCDC53. Interacts (via WHD1 region) with WASH1; the interaction is direct.By similarity

Protein-protein interaction databases

IntActiQ6PGL7. 3 interactions.
MINTiMINT-1864605.

Structurei

3D structure databases

ProteinModelPortaliQ6PGL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi221 – 2288Poly-Glu
Compositional biasi443 – 45311Poly-AspAdd
BLAST
Compositional biasi661 – 6655Poly-Asp
Compositional biasi780 – 7834Poly-Ser

Sequence similaritiesi

Belongs to the FAM21 family.Curated

Phylogenomic databases

eggNOGiNOG76301.
GeneTreeiENSGT00510000047694.
HOGENOMiHOG000112469.
HOVERGENiHBG055529.
InParanoidiQ6PGL7.
KOiK18462.
OMAiHSDNDQN.
OrthoDBiEOG7GBFWP.
PhylomeDBiQ6PGL7.
TreeFamiTF329309.

Family and domain databases

InterProiIPR027308. FAM21.
IPR029341. FAM21/CAPZIP.
[Graphical view]
PANTHERiPTHR21669:SF4. PTHR21669:SF4. 1 hit.
PfamiPF15255. CAP-ZIP_m. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6PGL7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRTSPDSER PPASEPVWER PWSVEEIRRS SQNWSLAADA GLLQFLQEFS
60 70 80 90 100
QQTISRTHEI KKQVDGLIQE TKATHCRLHN VFNDFLMLSN TQFIENRVYD
110 120 130 140 150
EEVEEQVLKA EAEKAEQEKT REQKEIDLIP KVQEAVNYGL QVLDSAFEQL
160 170 180 190 200
DIKAGNSDSE EDDANERVDL ILEPKDLYID RPLPYLIGSK LFMEQEDVGL
210 220 230 240 250
GELSSEEGSV GSDRGSIVDS EDEKEEEESD EDFASHSDND QNQHTTQISD
260 270 280 290 300
EEEDDDGDLF ADSEKEGDDI EDIEESAKSK RPTSFADELA ARIKGDISNQ
310 320 330 340 350
RKEGQTDGKP QKTVKEKKER RTPADDEEDI LFPPPTLTDE DFSPFGSRGG
360 370 380 390 400
LFSNGQGLFD DEDESDLFKE APRARPAQAP VSEELPPSPK PGKKIPAGAV
410 420 430 440 450
SVLLGHPDVS GSTSAPSLKE LQKHGQPTPG KSSHLPTPAG LFDDDDNDND
460 470 480 490 500
EDDNNFFMPS SSKPSKTDKV KSTAIIFDDD EGDLFKEKAE ALPAASVSQT
510 520 530 540 550
HESKTRADKT IALPSSKNLK LVSETKTQKG LFSDEEDSED LFSSQSSSKP
560 570 580 590 600
KSASLPSSQP PTSVSLFGDE DEEDSLFGSA AAKKQTSSLQ PQSQEKAKPS
610 620 630 640 650
EQPSKKTSAL LFSSDEEDQW NIADSHTKLA SDNKSKGELW DSGATQGQEA
660 670 680 690 700
KAVKKTNLFE DDDDDEVDLF AIAKDSQKKT QRTSLLFEDD AESGSSLFGL
710 720 730 740 750
PPTSVPSATT KKESVPKVPL LFSDEEDSEV PSGVKPEDLK VDNARVSPEV
760 770 780 790 800
GSADVASIAQ KEGLLPASDQ EAGGPSDIFS SSSPLDKGAK GRTRTVLSLF
810 820 830 840 850
DEDEDKVEDE SSTCAPQDGR EKGLKTDSRP KSTGVFQDEE LLFSHKLQKD
860 870 880 890 900
NDPDVDLFAG TKKIRSSVPS GGSLFGDDED DDLFSSAKTQ PVVPEKKGTL
910 920 930 940 950
KKDHPVSLKN QDPLDSTQGS KEKSTWKTEP AQDSSGLTPF KSREPSSRIG
960 970 980 990 1000
KIQANLAINP AALLPTVALQ IPGTKPVSSE LAFPSSEPGR SHILESVPTL
1010 1020 1030 1040 1050
PGSVEAGVSF DLPAQADTLH SANKSRVKVR GKRRPQTRAA RRLAAQESSE
1060 1070 1080 1090 1100
AEDVTVDRGP VAQLSSSPVL PNGHQPLLQP RMASGQTSSE TATAPPWEGG
1110 1120 1130 1140 1150
PVLSAADRSF FVKSRPQTGN EADLFDSGDI FPKSRGSQSV EGAGVMAGEP
1160 1170 1180 1190 1200
PSHSSGGRKE KSLAFPDLSE GSSTEDLFQS VKPRAAKNRN PFPLLEDEED
1210 1220 1230 1240 1250
LFADPRGKKN ERKPDSHQDS VSKTHDIFED DIFATEAIKP FPKKREKGRT
1260 1270 1280 1290 1300
LEPNLFDDNI DIFADLTVKP KEKSKKKVAA KSMFDDDTDD IFSSGLQAKA
1310 1320 1330
SKPKSQSAEA ASEQRSEHKV ASIFDDPLNA FGSQ
Length:1,334
Mass (Da):145,311
Last modified:July 5, 2004 - v1
Checksum:iAD35C3FFAC27C159
GO
Isoform 2 (identifier: Q6PGL7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     866-952: SSVPSGGSLF...REPSSRIGKI → V

Note: No experimental confirmation available.

Show »
Length:1,248
Mass (Da):136,082
Checksum:i410A9852587F5DB7
GO

Sequence cautioni

The sequence AAH49979.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti385 – 3851L → S in AAH49979. (PubMed:15489334)Curated
Sequence conflicti556 – 5561P → S in AAH49979. (PubMed:15489334)Curated
Sequence conflicti630 – 6301A → V in BAC65602. (PubMed:12693553)Curated
Sequence conflicti910 – 9101N → S in BAC29966. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei866 – 95287SSVPS…RIGKI → V in isoform 2. 1 PublicationVSP_030949Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK122320 Transcribed RNA. Translation: BAC65602.2.
AK163765 mRNA. Translation: BAE37485.1.
AK038318 mRNA. Translation: BAC29966.1.
BC049979 mRNA. Translation: AAH49979.1. Sequence problems.
BC056942 mRNA. Translation: AAH56942.1.
CCDSiCCDS20450.1. [Q6PGL7-1]
RefSeqiNP_080861.2. NM_026585.3. [Q6PGL7-1]
UniGeneiMm.28524.

Genome annotation databases

EnsembliENSMUST00000036759; ENSMUSP00000038983; ENSMUSG00000024104. [Q6PGL7-1]
GeneIDi28006.
KEGGimmu:28006.
UCSCiuc009djs.2. mouse. [Q6PGL7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK122320 Transcribed RNA. Translation: BAC65602.2 .
AK163765 mRNA. Translation: BAE37485.1 .
AK038318 mRNA. Translation: BAC29966.1 .
BC049979 mRNA. Translation: AAH49979.1 . Sequence problems.
BC056942 mRNA. Translation: AAH56942.1 .
CCDSi CCDS20450.1. [Q6PGL7-1 ]
RefSeqi NP_080861.2. NM_026585.3. [Q6PGL7-1 ]
UniGenei Mm.28524.

3D structure databases

ProteinModelPortali Q6PGL7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q6PGL7. 3 interactions.
MINTi MINT-1864605.

PTM databases

PhosphoSitei Q6PGL7.

Proteomic databases

MaxQBi Q6PGL7.
PaxDbi Q6PGL7.
PRIDEi Q6PGL7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000036759 ; ENSMUSP00000038983 ; ENSMUSG00000024104 . [Q6PGL7-1 ]
GeneIDi 28006.
KEGGi mmu:28006.
UCSCi uc009djs.2. mouse. [Q6PGL7-1 ]

Organism-specific databases

CTDi 28006.
MGIi MGI:106463. Fam21.
Rougei Search...

Phylogenomic databases

eggNOGi NOG76301.
GeneTreei ENSGT00510000047694.
HOGENOMi HOG000112469.
HOVERGENi HBG055529.
InParanoidi Q6PGL7.
KOi K18462.
OMAi HSDNDQN.
OrthoDBi EOG7GBFWP.
PhylomeDBi Q6PGL7.
TreeFami TF329309.

Miscellaneous databases

ChiTaRSi D6Wsu116e. mouse.
NextBioi 306510.
PROi Q6PGL7.
SOURCEi Search...

Gene expression databases

Bgeei Q6PGL7.
ExpressionAtlasi Q6PGL7. baseline and differential.
Genevestigatori Q6PGL7.

Family and domain databases

InterProi IPR027308. FAM21.
IPR029341. FAM21/CAPZIP.
[Graphical view ]
PANTHERi PTHR21669:SF4. PTHR21669:SF4. 1 hit.
Pfami PF15255. CAP-ZIP_m. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Head and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6 and FVB/N-3.
    Tissue: Brain and Mammary tumor.
  4. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-747, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157; SER-159; SER-284; SER-388; SER-533; SER-613; SER-614; SER-723; SER-747; SER-1169; SER-1172; SER-1173 AND SER-1333, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-614 AND SER-747, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiFAM21_MOUSE
AccessioniPrimary (citable) accession number: Q6PGL7
Secondary accession number(s): Q3TQ99
, Q80TW8, Q80UQ4, Q8CAP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3