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Q6PGL7

- FAM21_MOUSE

UniProt

Q6PGL7 - FAM21_MOUSE

Protein

WASH complex subunit FAM21

Gene

Fam21

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes By similarity.By similarity

    GO - Biological processi

    1. retrograde transport, endosome to Golgi Source: UniProtKB

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    WASH complex subunit FAM21
    Gene namesi
    Name:Fam21
    Synonyms:D6Wsu116e, Kiaa0592
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:106463. Fam21.

    Subcellular locationi

    GO - Cellular componenti

    1. early endosome Source: UniProtKB
    2. early endosome membrane Source: UniProtKB-SubCell
    3. WASH complex Source: UniProtKB

    Keywords - Cellular componenti

    Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13341334WASH complex subunit FAM21PRO_0000317433Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei157 – 1571Phosphoserine1 Publication
    Modified residuei159 – 1591Phosphoserine1 Publication
    Modified residuei284 – 2841Phosphoserine1 Publication
    Modified residuei388 – 3881Phosphoserine1 Publication
    Modified residuei533 – 5331Phosphoserine1 Publication
    Modified residuei613 – 6131Phosphoserine1 Publication
    Modified residuei614 – 6141Phosphoserine2 Publications
    Modified residuei723 – 7231Phosphoserine1 Publication
    Modified residuei747 – 7471Phosphoserine3 Publications
    Modified residuei1169 – 11691Phosphoserine1 Publication
    Modified residuei1172 – 11721Phosphoserine1 Publication
    Modified residuei1173 – 11731Phosphoserine1 Publication
    Modified residuei1333 – 13331Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6PGL7.
    PaxDbiQ6PGL7.
    PRIDEiQ6PGL7.

    PTM databases

    PhosphoSiteiQ6PGL7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6PGL7.
    BgeeiQ6PGL7.
    GenevestigatoriQ6PGL7.

    Interactioni

    Subunit structurei

    Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH1, FAM21, KIAA1033, KIAA0196 and CCDC53. Interacts (via WHD1 region) with WASH1; the interaction is direct.By similarity

    Protein-protein interaction databases

    IntActiQ6PGL7. 3 interactions.
    MINTiMINT-1864605.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6PGL7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi221 – 2288Poly-Glu
    Compositional biasi443 – 45311Poly-AspAdd
    BLAST
    Compositional biasi661 – 6655Poly-Asp
    Compositional biasi780 – 7834Poly-Ser

    Sequence similaritiesi

    Belongs to the FAM21 family.Curated

    Phylogenomic databases

    eggNOGiNOG76301.
    GeneTreeiENSGT00510000047694.
    HOGENOMiHOG000112469.
    HOVERGENiHBG055529.
    InParanoidiQ6PGL7.
    OMAiHSDNDQN.
    OrthoDBiEOG7GBFWP.
    PhylomeDBiQ6PGL7.
    TreeFamiTF329309.

    Family and domain databases

    InterProiIPR027308. FAM21.
    IPR029341. FAM21/CAPZIP.
    [Graphical view]
    PANTHERiPTHR21669:SF4. PTHR21669:SF4. 1 hit.
    PfamiPF15255. CAP-ZIP_m. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6PGL7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNRTSPDSER PPASEPVWER PWSVEEIRRS SQNWSLAADA GLLQFLQEFS     50
    QQTISRTHEI KKQVDGLIQE TKATHCRLHN VFNDFLMLSN TQFIENRVYD 100
    EEVEEQVLKA EAEKAEQEKT REQKEIDLIP KVQEAVNYGL QVLDSAFEQL 150
    DIKAGNSDSE EDDANERVDL ILEPKDLYID RPLPYLIGSK LFMEQEDVGL 200
    GELSSEEGSV GSDRGSIVDS EDEKEEEESD EDFASHSDND QNQHTTQISD 250
    EEEDDDGDLF ADSEKEGDDI EDIEESAKSK RPTSFADELA ARIKGDISNQ 300
    RKEGQTDGKP QKTVKEKKER RTPADDEEDI LFPPPTLTDE DFSPFGSRGG 350
    LFSNGQGLFD DEDESDLFKE APRARPAQAP VSEELPPSPK PGKKIPAGAV 400
    SVLLGHPDVS GSTSAPSLKE LQKHGQPTPG KSSHLPTPAG LFDDDDNDND 450
    EDDNNFFMPS SSKPSKTDKV KSTAIIFDDD EGDLFKEKAE ALPAASVSQT 500
    HESKTRADKT IALPSSKNLK LVSETKTQKG LFSDEEDSED LFSSQSSSKP 550
    KSASLPSSQP PTSVSLFGDE DEEDSLFGSA AAKKQTSSLQ PQSQEKAKPS 600
    EQPSKKTSAL LFSSDEEDQW NIADSHTKLA SDNKSKGELW DSGATQGQEA 650
    KAVKKTNLFE DDDDDEVDLF AIAKDSQKKT QRTSLLFEDD AESGSSLFGL 700
    PPTSVPSATT KKESVPKVPL LFSDEEDSEV PSGVKPEDLK VDNARVSPEV 750
    GSADVASIAQ KEGLLPASDQ EAGGPSDIFS SSSPLDKGAK GRTRTVLSLF 800
    DEDEDKVEDE SSTCAPQDGR EKGLKTDSRP KSTGVFQDEE LLFSHKLQKD 850
    NDPDVDLFAG TKKIRSSVPS GGSLFGDDED DDLFSSAKTQ PVVPEKKGTL 900
    KKDHPVSLKN QDPLDSTQGS KEKSTWKTEP AQDSSGLTPF KSREPSSRIG 950
    KIQANLAINP AALLPTVALQ IPGTKPVSSE LAFPSSEPGR SHILESVPTL 1000
    PGSVEAGVSF DLPAQADTLH SANKSRVKVR GKRRPQTRAA RRLAAQESSE 1050
    AEDVTVDRGP VAQLSSSPVL PNGHQPLLQP RMASGQTSSE TATAPPWEGG 1100
    PVLSAADRSF FVKSRPQTGN EADLFDSGDI FPKSRGSQSV EGAGVMAGEP 1150
    PSHSSGGRKE KSLAFPDLSE GSSTEDLFQS VKPRAAKNRN PFPLLEDEED 1200
    LFADPRGKKN ERKPDSHQDS VSKTHDIFED DIFATEAIKP FPKKREKGRT 1250
    LEPNLFDDNI DIFADLTVKP KEKSKKKVAA KSMFDDDTDD IFSSGLQAKA 1300
    SKPKSQSAEA ASEQRSEHKV ASIFDDPLNA FGSQ 1334
    Length:1,334
    Mass (Da):145,311
    Last modified:July 5, 2004 - v1
    Checksum:iAD35C3FFAC27C159
    GO
    Isoform 2 (identifier: Q6PGL7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         866-952: SSVPSGGSLF...REPSSRIGKI → V

    Note: No experimental confirmation available.

    Show »
    Length:1,248
    Mass (Da):136,082
    Checksum:i410A9852587F5DB7
    GO

    Sequence cautioni

    The sequence AAH49979.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti385 – 3851L → S in AAH49979. (PubMed:15489334)Curated
    Sequence conflicti556 – 5561P → S in AAH49979. (PubMed:15489334)Curated
    Sequence conflicti630 – 6301A → V in BAC65602. (PubMed:12693553)Curated
    Sequence conflicti910 – 9101N → S in BAC29966. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei866 – 95287SSVPS…RIGKI → V in isoform 2. 1 PublicationVSP_030949Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK122320 Transcribed RNA. Translation: BAC65602.2.
    AK163765 mRNA. Translation: BAE37485.1.
    AK038318 mRNA. Translation: BAC29966.1.
    BC049979 mRNA. Translation: AAH49979.1. Sequence problems.
    BC056942 mRNA. Translation: AAH56942.1.
    CCDSiCCDS20450.1. [Q6PGL7-1]
    RefSeqiNP_080861.2. NM_026585.3. [Q6PGL7-1]
    UniGeneiMm.28524.

    Genome annotation databases

    EnsembliENSMUST00000036759; ENSMUSP00000038983; ENSMUSG00000024104. [Q6PGL7-1]
    GeneIDi28006.
    KEGGimmu:28006.
    UCSCiuc009djs.2. mouse. [Q6PGL7-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK122320 Transcribed RNA. Translation: BAC65602.2 .
    AK163765 mRNA. Translation: BAE37485.1 .
    AK038318 mRNA. Translation: BAC29966.1 .
    BC049979 mRNA. Translation: AAH49979.1 . Sequence problems.
    BC056942 mRNA. Translation: AAH56942.1 .
    CCDSi CCDS20450.1. [Q6PGL7-1 ]
    RefSeqi NP_080861.2. NM_026585.3. [Q6PGL7-1 ]
    UniGenei Mm.28524.

    3D structure databases

    ProteinModelPortali Q6PGL7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q6PGL7. 3 interactions.
    MINTi MINT-1864605.

    PTM databases

    PhosphoSitei Q6PGL7.

    Proteomic databases

    MaxQBi Q6PGL7.
    PaxDbi Q6PGL7.
    PRIDEi Q6PGL7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000036759 ; ENSMUSP00000038983 ; ENSMUSG00000024104 . [Q6PGL7-1 ]
    GeneIDi 28006.
    KEGGi mmu:28006.
    UCSCi uc009djs.2. mouse. [Q6PGL7-1 ]

    Organism-specific databases

    CTDi 28006.
    MGIi MGI:106463. Fam21.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG76301.
    GeneTreei ENSGT00510000047694.
    HOGENOMi HOG000112469.
    HOVERGENi HBG055529.
    InParanoidi Q6PGL7.
    OMAi HSDNDQN.
    OrthoDBi EOG7GBFWP.
    PhylomeDBi Q6PGL7.
    TreeFami TF329309.

    Miscellaneous databases

    ChiTaRSi D6Wsu116e. mouse.
    NextBioi 306510.
    PROi Q6PGL7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6PGL7.
    Bgeei Q6PGL7.
    Genevestigatori Q6PGL7.

    Family and domain databases

    InterProi IPR027308. FAM21.
    IPR029341. FAM21/CAPZIP.
    [Graphical view ]
    PANTHERi PTHR21669:SF4. PTHR21669:SF4. 1 hit.
    Pfami PF15255. CAP-ZIP_m. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Head and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6 and FVB/N-3.
      Tissue: Brain and Mammary tumor.
    4. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-747, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157; SER-159; SER-284; SER-388; SER-533; SER-613; SER-614; SER-723; SER-747; SER-1169; SER-1172; SER-1173 AND SER-1333, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-614 AND SER-747, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiFAM21_MOUSE
    AccessioniPrimary (citable) accession number: Q6PGL7
    Secondary accession number(s): Q3TQ99
    , Q80TW8, Q80UQ4, Q8CAP0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3